Article
Cell Biology
Jinshan Xu, Shuxiong Zeng, Jun Li, Li Gao, Wenjun Le, Xin Huang, Guandan Wang, Bingdi Chen, Zhensheng Zhang, Chuanliang Xu
Summary: The study developed a novel UCT method called Nano-cytology by mixing urine with mNPs to improve the sensitivity of bladder cancer detection. The Nano-cytology assay showed significantly higher sensitivity compared to the UCT assay, and the combination of the two approaches further increased sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Letter
Cell Biology
Lidia Castillo-Gazquez, Enni C. Vera-Diaz, Patricia Munoz-Hernandez, Jose A. Jimenez-Heffernan
Summary: This article reports the presence of a Curschmann's spiral in a urine sample, emphasizing the importance of pathologists searching for them in this unusual site.
Review
Oncology
Marine Charpentier, Charly Gutierrez, Thierry Guillaudeux, Gregory Verhoest, Remy Pedeux
Summary: Bladder cancer is a common and potentially fatal disease that requires early detection and treatment. Urine tests are becoming increasingly popular as a noninvasive and easy method for diagnosing and monitoring the progression of bladder cancer.
Article
Oncology
Monika Kujdowicz, Brygida Mech, Karolina Chrabaszcz, Piotr Chlosta, Krzysztof Okon, Kamilla Malek
Summary: This study used FTIR to analyze patients' cytology samples, revealing a correlation between normal cell types and morphology, as well as identifying protein content variation in bladder cancer cells. Spectral classification using PLS-DA showed that voided urine cytology may be helpful in bladder cancer diagnosis.
Article
Virology
Tomomi Nakagawa, Kazuyoshi Shigehara, Yuki Kato, Shohei Kawaguchi, Hiroki Nakata, Taito Nakano, Kouji Izumi, Yoshifumi Kadono, Atsushi Mizokami
Summary: Although urine and bladder washing samples are commonly used for cytological evaluation of the bladder, their suitability for investigating HPV prevalence in the urinary bladder has been unknown. This study found that the use of washing samples appears to be applicable in screening HPV infection in the urinary bladder, as HPV-DNA detected in these samples may frequently originate from the bladder.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Oncology
Paolo Piatti, Yap Ching Chew, Michiko Suwoto, Taikun Yamada, Benjamin Jara, Xi-Yu Jia, Wei Guo, Saum Ghodoussipour, Siamak Daneshmand, Hamed Ahmadi, Jeffrey Rice, Jeffrey Bhasin, Faith Holloway, Yvonne Tsai, Yoshitomo Chihara, Gangning Liang
Summary: Bladder CARE is a highly sensitive and non-invasive test for detecting bladder cancer in urine samples. By measuring methylation levels of specific biomarkers, it has great potential for early detection and routine screening of BC patients. The test has a low limit of detection, reduces unnecessary invasive testing, and improves patient monitoring.
CLINICAL EPIGENETICS
(2021)
Article
Biochemistry & Molecular Biology
Tomasz Ligor, Przemyslaw Adamczyk, Tomasz Kowalkowski, Ileana Andreea Ratiu, Anna Wenda-Piesik, Boguslaw Buszewski
Summary: In this study, headspace solid phase microextraction (SPME) and gas chromatography time of flight mass spectrometry (GCxGC TOF MS) were used to analyze the volatile organic compounds (VOCs) in urine samples of bladder cancer patients and healthy individuals. The results showed that there were different VOC profiles between bladder cancer patients and healthy individuals. Several potential biomarkers were identified.
Review
Biochemistry & Molecular Biology
Vincenzo Fiorentino, Cristina Pizzimenti, Mariausilia Franchina, Esther Diana Rossi, Pietro Tralongo, Angela Carlino, Luigi Maria Larocca, Maurizio Martini, Guido Fadda, Francesco Pierconti
Summary: The management of NMIBC requires monitoring and evaluation methods such as urinary cytology, cystoscopy, and radiological assessment of the upper urinary tract. Urine cytology and methylation analysis are commonly used methods with high sensitivity for detecting high-grade bladder carcinoma and carcinoma in situ. The combined use of urine cytology and methylation analysis can diagnose high-grade bladder carcinoma and predict clinical and histological recurrences in doubtful cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Karla B. Pena, Francesc Riu, Anna Hernandez, Carmen Guilarte, Joan Badia, David Parada
Summary: Urothelial bladder cancer is a heterogeneous disease with high recurrence and progression rates. This study evaluated the usefulness of a urine DNA methylation test in the follow-up of urothelial bladder cancer patients with atypical urine cytology. The study found a positive correlation between the DNA methylation test and the diagnosis of urothelial cell atypia.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Makito Miyake, Nobutaka Nishimura, Sayuri Ohnishi, Yuki Oda, Takuya Owari, Kenta Ohnishi, Yosuke Morizawa, Shunta Hori, Daisuke Gotoh, Yasushi Nakai, Kazumasa Torimoto, Tomomi Fujii, Nobumichi Tanaka, Kiyohide Fujimoto
Summary: This study investigated the potential diagnostic and prognostic values of urine Nectin-2 and Nectin-4 in bladder cancer (BCa). The results showed that urine Nectin-4 levels were significantly higher than Nectin-2 levels. Both Nectin-2 and Nectin-4 had higher sensitivity than cytology for diagnosing BCa.
Article
Medical Laboratory Technology
Nadira Narine, Durgesh Rana, David Shelton, Dina Awad, Helena O'Flynn, Eleanor R. Jones, Neil A. J. Ryan, Emma J. Crosbie
Summary: Novel diagnostics have shown potential to differentiate malignant cells of different aetiologies in urogenital biofluid samples based on distinctive morphology and immunoprofiles. This can improve diagnostic pathways in complex cases with dual urogenital primaries.
DIAGNOSTIC CYTOPATHOLOGY
(2022)
Article
Oncology
Tomaz Smrkolj, Urska Cegovnik Primozic, Teja Fabjan, Sasa Sterpin, Josko Osredkar
Summary: This study evaluated the performance of XBC and VUC in the follow-up of bladder tumors, finding that XBC has higher sensitivity and negative predictive value in predicting malignant histopathological and cystoscopic findings, suggesting it could be used to increase follow-up cystoscopy time intervals.
RADIOLOGY AND ONCOLOGY
(2021)
Review
Urology & Nephrology
Marc Sbizzera, Francoise Descotes, Theo Arber, Paul Neuville, Alain Ruffion
Summary: There is currently insufficient quality data to support cystoscopy and urinary cytology as effective tools for the diagnostic and surveillance of bladder cancer in neurogenic bladder patients. However, FISH analysis to detect chromosomal changes, and PCR for TERT and FGFR3 promoter mutation detection, associated or not with KRAS mutation detection, stand out as candidates of interest for bladder cancer detection in this specific population and should be prospectively tested.
WORLD JOURNAL OF UROLOGY
(2022)
Review
Pathology
Madelyn Lew
Summary: Urine cytology is an economical and convenient method for screening patients with urinary symptoms and monitoring those previously diagnosed with urothelial carcinoma. Familiarity with nonurothelial malignancies in urine cytology allows for optimal patient care.
ADVANCES IN ANATOMIC PATHOLOGY
(2022)
Article
Oncology
Laura Costas, Irene Onieva, Beatriz Pelegrina, Fatima Marin, Alvaro Carmona, Marta Lopez-Querol, Jon Frias-Gomez, Paula Peremiquel-Trillas, Jose Manuel Martinez, Eduard Dorca, Joan Brunet, Marta Pineda, Jordi Ponce, Xavier Matias-Guiu, Silvia de Sanjose, Francesc Xavier Bosch, Laia Alemany, Sonia Paytubi
Summary: This study evaluated the use of urine samples for the detection and molecular classification of endometrial cancer. Mutations in DNA from urine supernatant samples were identified in 100% of endometrial cancer cases, while only one control showed variants at a low frequency. The results suggest that urine samples may offer a user-friendly and reliable tool for endometrial cancer detection and classification.
CLINICAL CANCER RESEARCH
(2023)
