Article
Biochemistry & Molecular Biology
Aneta Ostrozka-Cieslik, Barbara Dolinska, Florian Ryszka
Summary: This study analyzed the protective effect of Cu2+ as a component of Biolasol preservative fluid in preventing nephron damage during graft storage period, and also investigated the effectiveness of copper in the presence of prolactin added to Biolasol fluid.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eileen Edgworth, Lisa Ernst, Zoltan Czigany, Turgay Saritas, Laura Sophie Zarnitz, Marc Wiartalla, Peter Boor, Eva Miriam Buhl, Rolf Rossaint, Rene H. Tolba, Benedict Doorschodt, Gregor Fabry, Christian Bleilevens
Summary: Normothermic machine perfusion (NMP) combined with optimized perfusate composition can improve the quality of kidney preservation, but the optimization of perfusates is still under research. Hemoglobin-based oxygen carriers (HBOCs), such as HBOC-301, can be an alternative to packed red blood cells (pRBC), but they can cause methemoglobin formation and induce hypoxia. The addition of ascorbic acid (VitC) as an antioxidant may have a beneficial effect. This study showed that HBOC-301 is inferior to pRBCs in a porcine kidney NMP model, regardless of the addition of VitC. Oxidative stress and fragmentation of hemoglobin polymers may contribute to these results, indicating the need for further research on cell-free oxygen carriers without these issues.
Article
Multidisciplinary Sciences
Tim C. van Smaalen, Danielle M. H. Beurskens, Jasper J. H. F. M. Kox, Rasheendra Polonia, Rein Vos, Hans Duimel, Willine J. van de Wetering, Carmen Lopez-Iglesias, Chris P. Reutelingsperger, L. W. Ernest van Heurn, Carine J. Peutz-Kootstra, Gerry A. F. Nicolaes
Summary: Extracellular histones are released in warm ischemia and the resulting damage can be prevented by blocking histone release. The release of extracellular histones in patients receiving a renal transplant is associated with worse graft outcome. The findings suggest that targeting histones may be beneficial in preventing renal injury during transplantation.
Article
Biochemistry & Molecular Biology
Hanno Maassen, Leonie H. Venema, Marc G. Weiss, Tobias M. Huijink, H. Sijbrand Hofker, Anna K. Keller, Tom E. Mollnes, Marco Eijken, Soren E. Pischke, Bente Jespersen, Harry van Goor, Henri G. D. Leuvenink
Summary: Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aimed to improve the flush-out and decrease ischemic injury by adding hydrogen sulphide (H2S) to the flush medium. The addition of H2S during flush out and static cold storage resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.
Review
Urology & Nephrology
Asel S. Arykbaeva, Dorottya K. de Vries, Jason B. Doppenberg, Marten A. Engelse, Thomas Hankemeier, Amy C. Harms, Leonie G. Wijermars, Alexander F. Schaapherder, Jaap A. Bakker, Rutger J. Ploeg, Ian P. J. Alwayn, Jan H. N. Lindeman
Summary: Normothermic machine perfusion (NMP) is a novel preservation strategy that maintains organs in a metabolically active state, providing superior organ preservation and allowing viability testing before transplantation. However, current perfusion protocols may not fully address the metabolic requirements of marginal donor grafts. Monitoring of metabolic status during NMP is important for optimal outcomes.
KIDNEY INTERNATIONAL
(2021)
Review
Medicine, General & Internal
Julie De Beule, Delphine Keppens, Hannelie Korf, Ina Jochmans
Summary: There is uncertainty about the composition of the perfusate for normothermic kidney perfusion, and the effect of different perfusate components or ischemic conditions on cytokine levels is unclear. Further research is needed to understand the role of leukocyte filters and the changes in cytokine levels during different ischemic conditions.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Laura Zarnitz, Benedict M. Doorschodt, Lisa Ernst, Aisa Hosseinnejad, Eileen Edgworth, Tamara Fechter, Alexander Theissen, Sonja Djudjaj, Peter Boor, Rolf Rossaint, Rene H. Tolba, Christian Bleilevens
Summary: Donor organ shortage has led to the use of marginal grafts, but normothermic machine perfusion (NMP) can assess and restore the viability of these grafts before transplantation. An alternative to autologous packed red blood cell (pRBC)-based perfusates is the cell- and oxygen carrier-free solution Ecosol, supplemented with taurine, which may prevent adverse effects. This study compared the effects of 6 h NMP using pRBC and Ecosol on porcine kidneys, and found that despite some tissue damage in the Ecosol group, functional parameters and oxygenation were similar to the pRBC group, and taurine improved antioxidant capacity in the Ecosol group.
Review
Biochemistry & Molecular Biology
Tom Darius, Jay Nath, Michel Mourad
Summary: The use of high-risk renal grafts for transplantation requires optimization of pretransplant preservation and assessment strategies to improve clinical outcomes and decrease organ discard rate. Oxygenation during hypothermic machine preservation is proposed as a resuscitative measure, providing a critical overview of the fundamentals and suggesting different strategies for clinical implementation.
Article
Biotechnology & Applied Microbiology
Bin Li, Jie Zhang, Chuanyan Shen, Tingting Zong, Cong Zhao, Yumin Zhao, Yunhua Lu, Siyue Sun, Hongli Zhu
Summary: This study demonstrates that within 6 hours of perfusion with pPolyHb, liver physiological and biochemical activities can be effectively maintained in vitro. The optimal addition ratio of pPolyHb was found to be 24%, which significantly reduced the apoptosis rate. This suggests that pPolyHb-based perfusate is feasible for NMP of rat livers and opens up possibilities for further research in this field.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aneta Ostrozka-Cieslik, Barbara Dolinska, Florian Ryszka
Summary: This study examined the protective effect of zinc and zinc-prolactin systems as additives to preservation solutions in preventing nephron damage caused by ischemia. Zinc at a dose of 1 μg/L showed minor effectiveness in nephron protection, while Zn2+ added with prolactin acted as a prolactin inhibitor. Addition of Zn(II) or Zn(II) with PRL to the Biolasol solution is not recommended.
Article
Surgery
Laura Mazilescu, Catherine Parmentier, Sangeetha N. Kalimuthu, Sujani Ganesh, Masataka Kawamura, Toru Goto, Yuki Noguchi, Markus Selzner, Trevor W. Reichman
Summary: A novel NESPP technique was developed and tested, showing that it can keep pancreas metabolically active and improve the outcomes of transplantation by allowing for assessment and repair of the organ.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Anatomy & Morphology
Nur Khatijah Mohd Zin, Hiroki Bochimoto, Daisuke Kondoh, Yo Ishihara, Hiroyoshi Iwata, Tatsuya Shonaka, Hiromichi Obara, Hiromi Sakai, Hiroyuki Furukawa, Naoto Matsuno
Summary: Midthermic machine perfusion with hemoglobin-based oxygen vesicles (HbV) can suppress ultrastructural damages in donor livers, especially for liver sinusoidal endothelial cells (LSECs).
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Surgery
William C. Chapman, Andrew S. Barbas, Anthony M. D'Alessandro, Rodrigo Vianna, Chandrashekhar A. Kubal, Peter Abt, Christopher Sonnenday, Rolf Barth, Josue Alvarez-Casas, Hasan Yersiz, Devin Eckhoff, Robert Cannon, Yuri Genyk, Linda Sher, Andrew Singer, Sandy Feng, Garrett Roll, Ari Cohen, Maria B. Doyle, Debra L. Sudan, David Al-Adra, Adeel Khan, Vijay Subramanian, Nader Abraham, Kim Olthoff, Akin Tekin, Lynn Berg, Constantin Coussios, Chris Morris, Lucy Randle, Peter Friend, Stuart J. Knechtle
Summary: This study compared the effects of conventional low-temperature storage and storage at physiological body temperature on liver transplantation. The results showed that normothermic machine perfusion (NMP) did not significantly reduce early posttransplant liver injury and function, but it may have greater benefits for high-risk donor livers.
Article
Gastroenterology & Hepatology
Hiroki Bochimoto, Yo Ishihara, Nur Khatijah Mohd Zin, Hiroyoshi Iwata, Daisuke Kondoh, Hiromichi Obara, Naoto Matsuno
Summary: This study investigated the ultrastructural changes in liver sinusoidal endothelial cells (LSEC) and the surrounding sinusoids after machine perfusion (MP). The results showed that midthermic MP (MMP) can alleviate ER damage in LSEC, restore the mitochondria morphology, and prevent stress-induced liver graft injury.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Medicine, General & Internal
Annemarie Weissenbacher, John P. Stone, Maria Letizia Lo Faro, James P. Hunter, Rutger J. Ploeg, Constantin C. Coussios, James E. Fildes, Peter J. Friend
Summary: This study investigated biomarkers, cells, and cytokines in the perfusate during normothermic machine preservation (NMP) of discarded human kidneys and found that perfusate and hemodynamic parameters correlated well with donor characteristics and measured biomarkers. The study suggests that these parameters can be used to assess kidney viability during NMP.
FRONTIERS IN MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Christine Joseph, Eva Maria Berghausen, Arnica Behringer, Bernhard Rauch, Henrik Ten Freyhaus, Leoni Luisa Gnatzy-Feik, Maximilian Krause, Dickson W. L. Wong, Peter Boor, Stephan Baldus, Marius Vantler, Stephan Rosenkranz
Summary: This study characterizes the effects of thrombin and Factor Xa on PASMCs and their involvement in pulmonary vascular remodeling through PAR-1 and PAR-2. Inhibition of PAR-1 and PAR-2 significantly reduces vascular muscularization and improves right ventricular function in PAH. These findings suggest that PARs, rather than their activators, could be potential targets for PAH treatment.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Sonja Djudjaj, Panagiotis Kavvadas, Niki Prakoura, Roman D. Buelow, Tiffany Migeon, Sandrine Placier, Christos E. Chadjichristos, Peter Boor, Christos Chatziantoniou
Summary: By overexpressing ICN3 in a transgenic mouse model, we found that Notch3 signaling promotes tubular cell proliferation, alignment of cell division, dedifferentiation and hyperplasia, leading to cystic kidney diseases and pre-neoplastic lesions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jan C. Kamp, Lavinia Neubert, Maximilian Ackermann, Helge Stark, Christopher Werlein, Jan Fuge, Axel Haverich, Alexandar Tzankov, Konrad Steinestel, Johannes Friemann, Peter Boor, Klaus Junker, Marius M. Hoeper, Tobias Welte, Florian Laenger, Mark P. Kuehnel, Danny D. Jonigk
Summary: There is an increased prevalence of pulmonary fibrosis in COVID-19 survivors, but the underlying molecular mechanisms are not well understood. This multicentric study found that there is a shift from pro-inflammatory to fibrogenic activity after the first week of hospitalization in severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pathology
Nassim Bouteldja, David Laurin Hoelscher, Barbara Mara Klinkhammer, Roman David Buelow, Johannes Lotz, Nick Weiss, Christoph Daniel, Kerstin Amann, Peter Boor
Summary: This research proposes a novel concept of stain augmentation to develop stain-independent CNNs for digital pathology. It outperforms state-of-the-art techniques and enables segmentation on immunohistochemical stainings without additional annotations, achieving performance comparable to the annotated stain. Examples of application in inflammation and fibrosis quantification are presented, indicating the effectiveness of stain augmentation in deep-learning segmentation algorithms.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Pathology
Katja Ermert, Eva M. Buhl, Barbara M. Klinkhammer, Juergen Floege, Peter Boor
Summary: In chronic kidney disease (CKD), the endothelial glycocalyx (EG) experiences structural and functional changes. The study investigated the ultrastructural alterations of the EG in different CKD models and found reduced binding and significant pathologic alterations in peritubular capillaries.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
David Schumacher, Adelina Curaj, Mareike Staudt, Sakine Simsekyilmaz, Isabella Kanzler, Peter Boor, Barbara Mara Klinkhammer, Xiaofeng Li, Octavian Bucur, Adnan Kaabi, Yichen Xu, Huabo Zheng, Pakhwan Nilcham, Alexander Schuh, Mihaela Rusu, Elisa A. Liehn
Summary: Myocardial infarction remains the leading cause of death in developed countries. Changes in the composition of the extracellular matrix, influenced by inflammatory cell recruitment, can affect collagen synthesis in myofibroblasts and alter the composition of the scar. Neutrophils sustain fibroblast proliferation, remodeling, differentiation, migration, and inflammation, while inhibiting fibrillar collagen expression. Mononuclear cells increase the synthesis of all collagen subtypes, while CCR2(-/-) scar tissue shows higher levels of collagen 13.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Urology & Nephrology
Ting Jia, Tong Xu, Bart Smeets, Eva Miriam Buhl, Marcus Johannes Moeller, Juergen Floege, Barbara Mara Klinkhammer, Peter Boor
Summary: The study suggests that PDGF-B and its receptor PDGFR-beta play a crucial role in the development and progression of FSGS by affecting the activation, proliferation, and fibrotic characteristics of PECs. The molecular crosstalk between podocytes and PECs mediated by PDGF-B and PDGFR-beta drives glomerulosclerosis and the progression of FSGS. Treatment with PDGF-B neutralizing antibody can improve kidney function and reduce the occurrence of FSGS.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Letter
Critical Care Medicine
Daniel Gagiannis, Carsten Hackenbroch, Wilhelm Bloch, Fabian Zech, Frank Kirchhoff, Sonja Djudjaj, Saskia von Stillfried, Roman Buelow, Peter Boor, Konrad Steinestel
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Han Wang, Antonia Margarethe Stehr, Jeeshan Singh, Leticija Zlatar, Arndt Hartmann, Katja Evert, Elisabeth Naschberger, Saskia von Stillfried, Peter Boor, Luis E. E. Munoz, Jasmin Knopf, Michael Stuerzl, Martin Herrmann
Summary: During inflammatory responses, neutrophils utilize various defense mechanisms, such as phagocytosis, degranulation, recruitment of immune cells, secretion of anti-microbials, and release of neutrophil extracellular traps (NETs). Detection of NETs in tissue sections can be challenging due to the high fluorescence signals emitted from condensed nuclear DNA. However, using anti-DNA-IgM antibodies can effectively detect the extended DNA patches of NETs, providing a new perspective for studying neutrophil-associated immune reactions in diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marcelo A. S. de Toledo, Xuhuang Fu, Tiago Maie, Eva M. Buhl, Katrin Goetz, Susanne Schmitz, Anne Kaiser, Peter Boor, Till Braunschweig, Nicolas Chatain, Ivan G. Costa, Tim H. Bruemmendorf, Steffen Koschmieder, Martin Zenke
Summary: In this study, human induced pluripotent stem cells were differentiated into mast cells, which exhibited characteristics of systemic mastocytosis disease. These cells can be used for disease modeling and drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Zarnitz, Benedict M. Doorschodt, Lisa Ernst, Aisa Hosseinnejad, Eileen Edgworth, Tamara Fechter, Alexander Theissen, Sonja Djudjaj, Peter Boor, Rolf Rossaint, Rene H. Tolba, Christian Bleilevens
Summary: Donor organ shortage has led to the use of marginal grafts, but normothermic machine perfusion (NMP) can assess and restore the viability of these grafts before transplantation. An alternative to autologous packed red blood cell (pRBC)-based perfusates is the cell- and oxygen carrier-free solution Ecosol, supplemented with taurine, which may prevent adverse effects. This study compared the effects of 6 h NMP using pRBC and Ecosol on porcine kidneys, and found that despite some tissue damage in the Ecosol group, functional parameters and oxygenation were similar to the pRBC group, and taurine improved antioxidant capacity in the Ecosol group.
Article
Computer Science, Interdisciplinary Applications
Isaac Shiri, Behrooz Razeghi, Alireza Vafaei Sadr, Mehdi Amini, Yazdan Salimi, Sohrab Ferdowsi, Peter Boor, Deniz Guenduez, Slava Voloshynovskiy, Habib Zaidi
Summary: A federated learning (FL) framework was developed for multi-institutional PET/CT image segmentation. The FL algorithms showed comparable performance to centralized learning methods and outperformed the single center-based baseline. The results have promising implications for HN tumor segmentation from PET/CT images.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2023)
Article
Mathematical & Computational Biology
Roman D. Buelow, David L. Hoelscher, Ivan G. Costa, Peter Boor
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2023)
Article
Gastroenterology & Hepatology
Mohamed Ramadan Mohamed, Johannes Haybaeck, Hanghang Wu, Huan Su, Matthias Bartneck, Cheng Lin, Mark V. Boekschoten, Peter Boor, Benjamin Goeppert, Christian Rupp, Pavel Strnad, Roger J. Davis, Francisco Javier, Christian Trautwein
Summary: This study investigated the role of JNK1/2 during cholestasis and its hepatocyte-specific function. The results showed that Jnk1 and Jnk2 work together to protect hepatocytes from cholestatic liver disease by controlling Apelin signalling. Blocking Apelin signalling attenuated liver injury and fibrosis induced by cholestasis. Targeting Jnk1/2 in hepatocytes may be a new molecular strategy for treating cholestatic liver disease.
Meeting Abstract
Transplantation
Ting Jia, Tong Xu, Bart Smeets, Marcus Johannes Moeller, Juergen Floege, Barbara Mara Klinkhammer, Peter Boor
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)