Article
Biology
Chun-Yuh Huang, Daniela M. Loo, Weiyong Gu
Summary: Loss of proteoglycan (PG) is a potential factor responsible for intervertebral disc (IVD) degeneration. A new mathematical model of GAG biosynthesis was developed for IVD cells, showing that glycolytic enzymes significantly enhance GAG biosynthesis, especially under low glucose supply. Increasing intracellular glutamine concentration or the activity of glutamine:fructose-6-phosphate amidotransferase in the hexamine pathway also promotes GAG biosynthesis. This study enhances our understanding of the relationship between glycolysis and PG biosynthesis in IVD cells and provides a theoretical framework for developing new preventive and treatment strategies for IVD degeneration.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Correction
Materials Science, Biomaterials
Matthew D. Harmon, Daisy M. Ramos, D. Nithyadevi, Rosalie Bordett, Swetha Rudraiah, Syam P. Nukavarapu, Isaac L. Moss, Sangamesh G. Kumbar
Summary: This article discusses the challenges, innovations, and future directions in using functional biomaterials and structures for intervertebral disc repair and regeneration.
BIOMATERIALS SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Hiroki Ohnishi, Zhongying Zhang, Takashi Yurube, Yoshiki Takeoka, Yutaro Kanda, Ryu Tsujimoto, Kunihiko Miyazaki, Tomoya Matsuo, Masao Ryu, Naotoshi Kumagai, Kohei Kuroshima, Yoshiaki Hiranaka, Ryosuke Kuroda, Kenichiro Kakutani
Summary: This study investigated the effects of AdipoRon on human intervertebral disc (IVD) nucleus pulposus (NP) cells using a three-dimensional in vitro culturing system, and also examined the effects of AdipoRon on rat tail IVD tissues using an in vivo puncture-induced IVD degeneration model. The results demonstrated that AdipoRon suppressed the gene expression of proinflammatory factors and phosphorylation of p65 via the AMPK pathway, thereby alleviating IVD degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Mohamed Kamal Mesregah, Michael Repajic, Paul Mgbam, Zoe Fresquez, Jeffrey C. Wang, Zorica Buser
Summary: This study evaluated the trends and patterns of cervical degenerative disc disease in different age groups of symptomatic patients. The results showed that the number and severity of degenerated intervertebral discs increased significantly with age.
EUROPEAN SPINE JOURNAL
(2022)
Article
Medicine, General & Internal
Turkhun Cetin, Sevket Kahraman, Volkan Kizilgoz, Sonay Aydin
Summary: The main purpose of this study was to evaluate the intervertebral disc height and degeneration between the normal group and the group with disc herniation at the L4-L5 level using MRI and the Pfirrmann grading system. The results showed that the disc height in the herniated group was lower than in the normal group, and the herniated group had higher Pfirrman scores in terms of disc degeneration compared to the normal group.
Article
Endocrinology & Metabolism
Zhiqi Wang, Tao Xue, Tongxing Zhang, Xuehui Wang, Hui Zhang, Zhongyu Gao, Qiang Zhou, Erke Gao, Tao Zhang, Zhaoyang Li
Summary: This study used Raman spectroscopy and histological methods to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP). They found that proteoglycans and collagen undergo changes in content and structure during degeneration, providing further understanding of the mechanism of cervical disc herniation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Long Zhang, Guicheng Du, Bogang Teng, Xueqing Shi, Xuemei He, Na Li, Yu Chen, Ren Xu
Summary: This study established a mouse model of intervertebral disc degeneration by needle puncture, and evaluated the morphological and molecular changes. Accurate localization of puncture positions was achieved by injecting colored perfusates, improving the accuracy and safety of the surgery.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Wentao Zhang, Ming Yang, Tianze Sun, Jing Zhang, Yantao Zhao, Jingmin Li, Zhonghai Li
Summary: Degenerative disc disease (DDD) is a significant issue associated with chronic back pain. Direct protein injection therapy has limitations due to its short efficacy. Encapsulating protein in hollow manganese dioxide (MnO2) microspheres may solve the problem of rapid drug release and have a synergistic effect on degrading pro-inflammatory factors in the DDD microenvironment, providing a new therapeutic approach.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Chang Kyu Lee, Dong Hwa Heo, Hungtae Chung, Eun Ji Roh, Anjani Darai, Jae Won Kyung, Hyemin Choi, Su Yeon Kwon, Basanta Bhujel, Inbo Han
Summary: Intervertebral disc degeneration is a leading cause of chronic low back pain, with current treatments including conservative and surgical approaches. However, these treatments cannot fully restore degenerated discs. Biological approaches like growth factor injection and tissue engineering show promising potential for promoting disc regeneration and are areas of ongoing research. Advancements in tissue engineering have opened up new possibilities for regenerative therapies for chronic discogenic low back pain.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Marco Brenneis, Zsuzsa Jenei-Lanzl, Johannes Kupka, Sebastian Braun, Marius Junker, Frank Zaucke, Marcus Rickert, Andrea Meurer
Summary: This study found that there is a correlation between the expression of adrenoceptors and intervertebral disc degeneration (IVDD) in human intervertebral discs, with certain receptors showing a positive correlation with Pfirrmann grade, indicating a potential role of the sympathetic nervous system in the pathogenesis of IVDD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Alexandra Alcantara Guardado, Alexander Baker, Andrew Weightman, Judith A. Hoyland, Glen Cooper
Summary: Lumbar disc herniation is a common condition that causes lower back pain and sciatica. Microdiscectomy is the most used surgical technique, but it can result in defects in the annulus fibrosus. This paper reviews commercially available products for mechanically closing the annulus and proposes a design specification for a mechanical annular closure strategy.
BIOENGINEERING-BASEL
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Fei Gao, Shui Liu, Xiaodong Zhang, Xiaoying Wang, Jue Zhang
Summary: The study proposed a computer-assisted method for grading intervertebral disc degeneration, which significantly improved the accuracy of disc grading by incorporating the push-pull regularization strategy. The application of this method in deep learning models has made the diagnosis of intervertebral disc degeneration more reliable.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2021)
Article
Biochemistry & Molecular Biology
Mengying Yang, Dingding Xiang, Song Wang, Weiqiang Liu
Summary: This study focuses on the accurate, synchronous, and noninvasive assessment of disc bulging and strains using the 3D digital image correlation (3D-DIC) system and the impact of creep on them. By applying different loads, the axial strains and lateral bulging of porcine cervical discs were measured. The results showed that load application reduced disc strains and the largest bulging occurred in the middle of the discs.
Article
Radiology, Nuclear Medicine & Medical Imaging
Naosuke Kamei, Toshio Nakamae, Kazuyoshi Nakanishi, Takayuki Tamura, Yuji Tsuchikawa, Taiki Morisako, Takahiro Harada, Toshiaki Maruyama, Nobuo Adachi
Summary: Intervertebral disc degeneration can be assessed using the signal ratio of MRI T2 values of the disc to the spinal cord T2 values, even under different imaging conditions. The signal ratio has higher equivalence in MRI assessments of disc degeneration than T2 signal intensity. The signal ratio is also significantly correlated with age and Pfirrmann grade.
EUROPEAN JOURNAL OF RADIOLOGY
(2022)
Article
Environmental Sciences
Rafal Staszkiewicz, Kamil Brys, Dorian Gladysz, Marcin Gralewski, Michal Garczarek, Marcin Gadzielinski, Jerzy Wieczorek, Wieslaw Marcol, Aleksander Ostenda, Beniamin Oskar Grabarek
Summary: This study revealed significant differences in the concentrations of potassium, copper, and iron in degenerated intervertebral discs compared to healthy discs. The concentrations of copper and iron were found to be dependent on the radiological advancement of the lesions, while the concentration of potassium was related to the degree of pain intensity.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Orthopedics
Rahul Gawri, Ryszard Bielecki, Eric W. Salter, Alena Zelinka, Toshikazu Shiba, Graham Collingridge, Andras Nagy, Rita A. Kandel
Summary: Inorganic polyphosphates (polyP) require calcium influx to regulate chondrocyte matrix production, likely through activating calcium signaling pathway. These findings help in the clinical translation of polyP into a therapeutic agent for cartilage repair.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Endocrinology & Metabolism
Jose L. Ramirez-GarciaLuna, Karla Rangel-Berridi, Ore-Oluwa Olasubulumi, Derek H. Rosenzweig, Janet E. Henderson, Rahul Gawri, Paul A. Martineau
Summary: This study investigated how immune cells influence bone repair using a mouse model. The results showed that mice exposed to subsequent fractures had significantly improved bone morphometric parameters and increased content of blood vessels, osteoclasts, and macrophages. These findings suggest that a transient increase in the inflammatory state during healing injury promotes faster bone remodelling and enhanced neo-angiogenesis.
CALCIFIED TISSUE INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Shannon Tansley, Sonali Uttam, Alba Urena Guzman, Moein Yaqubi, Alain Pacis, Marc Parisien, Haley Deamond, Calvin Wong, Oded Rabau, Nicole Brown, Lisbet Haglund, Jean Ouellet, Carlo Santaguida, Alfredo Ribeiro-da-Silva, Soroush Tahmasebi, Masha Prager-Khoutorsky, Jiannis Ragoussis, Ji Zhang, Michael W. Salter, Luda Diatchenko, Luke M. Healy, Jeffrey S. Mogil, Arkady Khoutorsky
Summary: In this study, the researchers used single-cell RNA sequencing to investigate the role of microglia in the development of chronic pain after peripheral nerve injury. They found that the injury induced the generation of a specific inflammatory microglia subtype in male mice, and there was increased proliferation of microglia in male as compared to female mice. They also observed time- and sex-specific transcriptional changes in different microglial subpopulations following the injury. Furthermore, they identified the apolipoprotein E (ApoE) gene as the top upregulated gene in mouse microglia at chronic time points after peripheral nerve injury, and polymorphisms in APOE gene in humans were associated with chronic pain. Analysis of human spinal cord microglia revealed a subpopulation with a disease-related transcriptional signature. These findings provide valuable insights into the role of microglia in pain and establish a link between ApoE and chronic pain.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Hosni Cherif, Matthew Mannarino, Alain Sarabia Pacis, Jiannis Ragoussis, Oded Rabau, Jean A. Ouellet, Lisbet Haglund
Summary: In this study, single-cell transcriptomic analysis was used to identify new biomarkers for nucleus pulposus (NP) and inner annulus fibrosis (iAF) cells in intervertebral discs (IVD). The analysis also provided insights into the cell populations within non-degenerating and degenerating IVD. The findings suggest potential functional differences linked to degeneration and among NP and iAF subpopulations, and also propose specific cell type and predictive IVD degeneration genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Dhanalakshmi Jeyachandran, Li Li, Rayan Fairag, Lisbet Haglund, Marta Cerruti
Summary: Scaffold porosity is crucial in bone tissue engineering, and this study proposes a simple technique to create dual-porosity scaffolds by introducing micropores into the PLGA matrix. The microporous scaffolds exhibit enhanced bioactivity, protein adsorption, and matrix deposition, indicating their potential for fast bone integration.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2022)
Article
Neurosciences
Moein Yaqubi, Julia Xiao Xuan Luo, Salma Baig, Qiao-Ling Cui, Kevin Petrecca, Haritha Desu, Catherine Larochelle, Elia Afanasiev, Jeffery A. Hall, Roy Dudley, Myriam Srour, Lisbet Haglund, Jean Ouellet, Miltiadis Georgiopoulos, Carlo Santaguida, Joshua A. Sonnen, Luke M. Healy, Jo Anne Stratton, Timothy E. Kennedy, Jack P. Antel
Summary: Morphological and molecular studies have shown that there is heterogeneity within the oligodendrocyte population in the human central nervous system. Through single-cell RNA sequencing, this study identified transcriptionally distinct subpopulations of mature oligodendrocytes in different regions and at different ages. The findings suggest that the heterogeneity of oligodendrocyte populations may be influenced by developmental and environmental factors.
Article
Orthopedics
Shangbin Cui, Wenyue Li, Graciosa Q. Teixeira, Cornelia Neidlinger-Wilke, Hans-Joachim Wilke, Lisbet Haglund, Hongwei Ouyang, R. Geoff Richards, Sibylle Grad, Mauro Alini, Zhen Li
Summary: This study investigates the spatial and temporal changes of aggrecan and collagen neoepitopes during intervertebral disc degeneration. The results show that different degeneration types are associated with specific neoepitope expression profiles, which may serve as biomarkers for early disc degeneration and indicators of different degeneration phenotypes.
Article
Cell Biology
Li Li, Kai Sheng, Matthew Mannarino, Peter Jarzem, Hosni Cherif, Lisbet Haglund
Summary: This study found that o-Vanillin affects cell communication between human mesenchymal stem cells (hMSCs) and intervertebral disc cells (DCs), enhances hMSC differentiation, and improves DC phenotype. o-Vanillin can increase EV release and uptake, as well as reduce the number of senescent cells.
Review
Orthopedics
Shirley N. Tang, Andres F. Bonilla, Nadeen O. Chahine, Aimee C. Colbath, Jeremiah T. Easley, Sibylle Grad, Lisbet Haglund, Christine L. Le Maitre, Victor Leung, Annette M. McCoy, Devina Purmessur, Simon Y. Tang, Stephan Zeiter, Lachlan J. Smith
Summary: Intervertebral disc degeneration is a common cause of low back pain, and appropriate preclinical models are crucial for research and treatment development. This article presents contrasting arguments for in vivo animal models and ex vivo organ culture models for disc studies, aiming to reach a consensus on model selection and implementation. The authors conclude that combining multiple model types and leveraging their unique advantages can lead to high-impact research in most cases.
Article
Orthopedics
D. G. Bisson, K. Sheng, S. Kocabas, D. D. Ocay, C. E. Ferland, N. Saran, J. A. Ouellet, L. Haglund
Summary: This study evaluated the association between facet joint cartilage and subchondral bone homeostasis, perceived back pain, and 3-dimensional spinal deformity in patients with adolescent idiopathic scoliosis (AIS). The results showed that facet joint cartilage degeneration was associated with decreased subchondral bone volume and quality, and strongly correlated to intervertebral axial rotation, kyphotic intervertebral angle, and back pain intensity. These findings suggest that facet joint degeneration is important for rotational instability, disease progression, and perceived back pain in AIS.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Biochemistry & Molecular Biology
Matthew Mannarino, Oliver Wu-Martinez, Kai Sheng, Li Li, Rodrigo Navarro-Ramirez, Peter Jarzem, Jean A. Ouellet, Hosni Cherif, Lisbet Haglund
Summary: This study aimed to determine if combining o-Vanillin and RG-7112 could more efficiently remove senescent cells and reduce inflammatory factors and pain mediators in degenerating intervertebral disc (IVD) cells. The results showed that the combination of these two senolytic drugs significantly reduced the amount of senescent IVD cells, proinflammatory cytokines, and neurotrophic factors. These findings support the potential of senolytics as a promising treatment for IVD-related low back pain.
Article
Pharmacology & Pharmacy
Xuan Li, Yin Liu, Shiyu Liu, Nicole Y. K. Li-Jessen, Lisbet Haglund, Baolin Huang, Jianyu Li
Summary: Tissue loss due to injury, surgery, and disease drives the development of new biomaterials for tissue repair and regeneration. A new generation of regenerative bioadhesives, with dual functions of seamless tissue adhesion and effective tissue repair, emerges at the intersection of bioadhesives and regenerative medicine. This perspective discusses the design principles of regenerative bioadhesives, considering both mechanical and biological aspects, and presents case studies of their application in load-bearing organs such as skin, tendon, and intervertebral discs. The article concludes with immediate opportunities and future perspectives for advancing the field of regenerative bioadhesives.
ADVANCED THERAPEUTICS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Christine Le Maitre, Joseph Snuggs, L. Beckman, Hosni Cherif, Simon Partridge, Lee Breakwell, Ashley Cole, Neil Chiverton, Chris Sammon, Lisbet Haglund
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Megan Cooke, Samantha Ozere, Thierry Pauyo, Lisbet Haglund, Derek Rosenzweig
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Clinical Neurology
Seunghwan Lee, Yuya Kawarai, Jean Ouellet, Lisbet Haglund, Moshe Szyf, Laura S. Stone