Journal
EUROPEAN RADIOLOGY
Volume 20, Issue 12, Pages 2917-2924Publisher
SPRINGER
DOI: 10.1007/s00330-010-1873-9
Keywords
Dual-source CT; CoronaryCTangiography; Biological dose; DNA double-strand breaks; gamma-H2AX
Funding
- Bundesministerium fur Forschung und Bildung, Bonn, Germany [BMBF 01 EV0708]
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To compare in vivo DNA lesions induced during helical and sequential coronary computed tomography angiography (CTA) and to evaluate the effect of CT parameters on double-strand break (DSB) levels. Thirty-six patients were examined with various CT protocols and modes (helical scan, n = 27; sequential scan, n = 9) either using a 64-slice dual-source or a 128-slice CT system. Blood samples were obtained before and 30 min after CT. Lymphocytes were isolated, stained against the phosphorylated histone variant gamma-H2AX, and DSBs were visualised by using fluorescence microscopy. DSB yields 30 min after CTA ranged from 0.04 to 0.71 per cell and showed a significant correlation to DLP (rho = 0.81, p < 0.00001). Median DSB yield and median DLP were significantly lower after sequential compared to helical CT examinations (0.11 vs. 0.37 DSBs/cell and 249 vs. 958 mGy cm, p < 0.00001). Additional calcium scoring led to an increase in DLP (p = 0.15) and DSB levels (p = 0.04). DSB levels normalised to the DLP showed a significant correlation to the attenuation of the blood (rho = 0.53, p = 0.01) and a negative correlation to the body mass index of the patients (rho = -0.37, p = 0.06). gamma-H2AX immunofluorescence microscopy allows one to determine dose-related effects on x-ray-induced DSB levels and to consider individual factors which cannot be monitored by physical dose measurements.
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