4.4 Article

Phytoanticipins from banana (Musa acuminata cv. Grande Naine) plants, with antifungal activity against Mycosphaerella fijiensis, the causal agent of black Sigatoka

Journal

EUROPEAN JOURNAL OF PLANT PATHOLOGY
Volume 126, Issue 4, Pages 459-463

Publisher

SPRINGER
DOI: 10.1007/s10658-009-9561-9

Keywords

Black Sigatoka; Diosgenin; Musa acuminata; Mycosphaerella fijiensis; Phytoanticipins; Saponins

Funding

  1. SAGARPA-CONACyT [2004-168]
  2. Royal Society International Partnership Award [2008/R1]
  3. Biotechnology and Biological Sciences Research Council [BBS/E/J/00000614] Funding Source: researchfish

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The previously detected antifungal activity against Mycosphaerella fijiensis of aqueous infusions of healthy banana (Musa acuminata cv. Grande Naine) leaves, suggested the production of phytoprotectants by the plant. The bioassay-guided VLC-purification of the lyophilized infusion of the leaves of 4-month old healthy banana (M. acuminata cv. Grande Naine) plants, resulted in the purification of a phytoanticipin with strong antifungal activity against M. fijiensis Morelet, the causal agent of black Sigatoka, the most destructive and devastating disease of bananas and plantains in the world. The LC-MS analysis of the purified phytoanticipin suggests a steroidal saponin structure with four sugar units attached to the C-3 position of a diosgenin-like aglycone. This represents the first report of phytoanticipins occurring in M. acuminata.

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