Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 738, Issue -, Pages 133-141Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2014.05.027
Keywords
1,8-dihydroxy-3-acetyl-6-methy1-9,10 anthraquinone; Nasopharyngeal carcinoma (NPC); iTRAQ; Radiosensitisation; Mitochondrial proteins; Bioinformatics
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Funding
- National Natural Science Foundation of China [30660047, 81060270]
- Guangxi Natural Science Foundation [0728112]
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In our preliminary study, 1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone (GXHSWAQ-1), synthesised according to the basic structure of emodin, exhibited a 1.58-fold radiosensitisation on nasopharyngeal carcinoma CNE-1 cells. This study demonstrated that its radiosensitisation activity was achieved by altering the mitochondrial structure: swollen volume, fragmented crista, and decreasing transmembrane potential (P < 0.01). Using isobaric tag for relative and absolute quantitation (iTRAQ) technology. 1396 proteins were identified, and the differentially expressed proteins were involved in metabolism, cell proliferation, angiogenesis, DNA repair process according to the biological process clustering results. Bioinformatic analysis showed that CDH1, RAC, CDC42 proteins might be mostly mitochondrial targets in the radiosensitisation process. Western blotting analyses verified the differential expression of these proteins. (C) 2014 Elsevier B.V. All rights reserved,
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