Article
Biochemistry & Molecular Biology
Piumi B. Wickramasinghe, Shuwen Qian, Lyndsey E. Langley, Chen Liu, Lin Jia
Summary: Accumulating evidence suggests that alcohol overconsumption is associated with insulin resistance, and this study investigated the role of hepatocyte toll-like receptor 4 (TLR4) in alcohol-induced insulin resistance. The findings indicate that hepatocyte TLR4 is both required and sufficient for the development of insulin resistance caused by alcohol overconsumption.
Article
Public, Environmental & Occupational Health
Yu Zhou, Wenhuo Xie, Linyao Wang, Xinyan Zhu, Jianbin Li, Libin Liu, Shuaijun Zhu, Lijing Wang
Summary: Our study identified some potential pharmacovigilance signals between hypoglycemia and TNF-alpha inhibitors, which warrants further validation.
Article
Oncology
Jun Ying, Haiyang Zhou, Zhiguo Wang, Qing You, Junnan Chen, Hao Lu, Jian Zhang
Summary: Background: Chemotherapy resistance is a major obstacle to the efficacy of chemotherapy in colon cancer. Therefore, improving the chemotherapy sensitivity of colorectal cancer cells is crucial for enhancing the prognosis of colon cancer patients. Methods: Cell viability of colorectal cancer cell lines was assessed using the CCK-8 assay. Toll-like receptor 4 (TLR4) expression in colorectal cancer cell lines was investigated through real-time PCR and western blot. The role of TLR4 in the stemness of colorectal cancer cell lines was analyzed by infecting cells with lentivirus containing TLR4 siRNA. Results: Aspirin was found to effectively enhance chemosensitivity and inhibit the stemness of CT26 and HCT116 colorectal cancer cell lines. Aspirin inhibited clone formation, reduced the volume and number of cell spheres, and down-regulated stemness-related genes. Moreover, aspirin also down-regulated TLR4 expression in colorectal cancer cells. TLR4 positive colorectal cancer cells demonstrated higher chemotherapy resistance potential and stronger stemness compared to TLR4 negative colorectal cancer cells. Conclusion: Our study indicates that aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Ahmed K. Aladhami, Christian A. Unger, Shannon L. Ennis, Diego Altomare, Hao Ji, Marion C. Hope, Kandy T. Velazquez, Reilly T. Enos
Summary: Macrophage-derived TNF-alpha does not play a significant role in the development of obesity, but can reduce TNF-alpha gene expression and circulating levels in adipose tissue, as well as modulate specific signaling pathway gene expression.
Review
Biochemistry & Molecular Biology
Suqing Bao, Fengbo Li, Lijun Duan, Junfeng Li, Xia Jiang
Summary: TSH may aggravate inflammation by increasing macrophage infiltration and activate the TLR4-associated inflammatory signaling pathway, thus interfering with insulin signals in liver tissues. Targeting TSH may have therapeutic benefits against metabolic disorders.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Shih-Yi Lin, Ya-Yu Wang, Cheng-Yi Chang, Chih-Cheng Wu, Wen-Ying Chen, Su-Lan Liao, Chun-Jung Chen
Summary: The emerging molecule TNF-alpha is reported to have inflammatory and metabolic roles. Studies have shown that the TNF-alpha receptor inhibitor R-7050 has neuroprotective, antihyperglycemic, and anti-inflammatory effects in stroke cases. This highlights a feasible way to combat stroke disease based on an anti-TNF therapy involving anti-inflammatory and metabolic mechanisms.
Review
Biotechnology & Applied Microbiology
Danieli F. Buccini, Beatriz C. Roriz, Julia M. Rodrigues, Octavio L. Franco
Summary: Antimicrobial peptides are important molecules in the organism's defense system. They can modulate the immune system and recognize molecules present in pathogens. Overstimulation of TLR4 can lead to uncontrolled inflammatory responses, which can be counteracted by antimicrobial peptides.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nikolett Bodi, Abigel Egyed-Kolumban, Benita Onhausz, Bence Pal Barta, Afnan AL Doghmi, Janos Balazs, Zita Szalai, Maria Bagyanszki
Summary: This study investigated the expression of Toll-like receptor 4 (TLR4) in myenteric neurons of different gut regions in a type 1 diabetes model. The results showed that TLR4 expression varied between different intestinal segments, with higher levels in diabetic rats. However, insulin treatment was found to have beneficial effects on TLR4 expression.
Review
Biochemistry & Molecular Biology
Natticha Sumneang, Pongpan Tanajak, Thura Tun Oo
Summary: Doxorubicin (Dox) is commonly used chemotherapeutic drug, but its cardiotoxicity reduces its therapeutic efficacy. Dox-induced cardiac inflammation is considered as one of the underlying mechanisms involved in Dox-induced cardiotoxicity. Understanding the role of the Toll-like receptor 4 (TLR4) signaling pathway in Dox-induced cardiac inflammation might be beneficial for developing potential therapeutic strategies.
Article
Chemistry, Medicinal
Yongsheng Zhang, Xinjie Liang, Xuefei Bao, Wei Xiao, Guoliang Chen
Summary: This review discusses TLR4 inhibitors that can bind directly to TLR4 or the TLR4/MD2 complex, as well as compounds that can downregulate the expression of TLR4. The involvement of specific residues in the binding of antagonistic ligands to the TLR4/MD2 complex provides useful information for structure-based TLR4 inhibitor design.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Changliang Zhu, Hong Tao, Shikuo Rong, Lifei Xiao, Xinxiao Li, Shucai Jiang, Baorui Guo, Lei Wang, Jiangwei Ding, Caibing Gao, Haigang Chang, Tao Sun, Feng Wang
Summary: Exendin-4 was effective in inhibiting cocaine-induced CPP, facilitating the extinction of cocaine-associated reward, and attenuating reinstatement of cocaine-induced behavior. These effects may be attributed to the inhibition of TLR4, TNF-alpha, and IL-1 beta expression.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pediatrics
Tianjing Liu, Haifeng Zong, Xiaoyu Chen, Sihang Li, Ziyun Liu, Xuewei Cui, Guoqiang Jia, Yongyan Shi
Summary: The study indicates that necroptosis is an essential pathological process of NEC, with TLR4 possibly acting as a stimulator. Inhibiting intestinal cell necroptosis may be a beneficial strategy in the treatment of NEC.
PEDIATRIC RESEARCH
(2022)
Review
Medicine, Research & Experimental
Jinrui Wei, Yan Zhang, Haopeng Li, Fuquan Wang, Shanglong Yao
Summary: This review focuses on the role and mechanism of TLR4 in various diseases, aiming to provide new research directions and therapeutic targets for clinical intervention.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Jie Zi, Fang Wang, Zebin Liu, Yunxia Wang, Liying Qiu, Guosheng Zhu, Henghua Li
Summary: This study aimed to determine the mechanism through which the expression of Toll-like receptor 4 (TLR4) influences the lipopolysaccharide (LPS)-induced inflammatory response, a condition that is associated with premature rupture of membranes (PROM). The results showed that suppressing TLR4 expression can mitigate inflammatory responses, thereby reducing the likelihood of premature rupture of membranes during pregnancy.
DISCOVERY MEDICINE
(2023)
Article
Endocrinology & Metabolism
Hai-Yun Guan, He-Xia Xia, Xiu-Ying Chen, Lu Wang, Zhi-Jing Tang, Wei Zhang
Summary: The activation of TLR4 was found to suppress CYP19A1 expression and E2 secretion in human granulosa cells via NF-κB signaling, which has implications for follicular development and estrogen synthesis regulation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Francisco Javier Ruiz-Ojeda, Jiefu Wang, Theresa Baecker, Martin Krueger, Samira Zamani, Simon Rosowski, Tim Gruber, Yasuhiro Onogi, Annette Feuchtinger, Tim J. Schulz, Reinhard Faessler, Timo D. Mueller, Cristina Garcia-Caceres, Matthias Meier, Matthias Blueher, Siegfried Ussar
Summary: The study shows that integrin signaling regulates white adipocyte insulin action and systemic metabolism. Loss of adipose integrin activity, similar to loss of adipose insulin receptors, leads to a lipodystrophy-like phenotype and systemic insulin resistance.
MOLECULAR METABOLISM
(2021)
Article
Endocrinology & Metabolism
Tomoko Tanaka, Tsutomu Wada, Kimie Uno, Saki Ogihara, Hiromi Ie, Akira Okekawa, Akari Ishikawa, Tetsuo Ito, Yuichiro Miyazawa, Azusa Sameshima, Yasuhiro Onogi, Hiroshi Tsuneki, Masakiyo Sasahara, Akitoshi Nakashima, Shigeru Saito, Toshiyasu Sasaoka
Summary: This study using a mouse model demonstrates that deletion of ER alpha in T cells results in impaired maternal adaptation of insulin secretion, changes in hepatokine profiles, and enhanced chronic inflammation in gWAT, shedding light on the pathogenesis of GDM.
Article
Endocrinology & Metabolism
Nir Goldstein, Hiroshi Tsuneki, Nikhil Bhandarkar, Eleonora Aimaretti, Yulia Haim, Kanta Kon, Kiyofumi Sato, Tsutomu Wada, Idit F. Liberty, Boris Kirshtein, Oleg Dukhno, Nitzan Maixner, Yftach Gepner, Toshiyasu Sasaoka, Assaf Rudich
Summary: This study suggests that human adipose tissue may be a direct target of the OxA-OxR1 system, with higher morning levels of Orexin being associated with improved metabolic characteristics.
Editorial Material
Gastroenterology & Hepatology
Yasuhiro Onogi, Siegfried Ussar
Article
Biochemistry & Molecular Biology
Zydrune Polianskyte-Prause, Tuomas A. Tolvanen, Sonja Lindfors, Kanta Kon, Laura C. Hautala, Hong Wang, Tsutomu Wada, Hiroshi Tsuneki, Toshiyasu Sasaoka, Sanna Lehtonen
Summary: Ebselen is a potential treatment for diabetes-related disorders. It inhibits SHIP2 to ameliorate insulin resistance and act as an antioxidant. Ebselen directly binds to and inhibits the activity of SHIP2, improving insulin sensitivity, protecting liver tissue, and improving glucose tolerance.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Takuya Okada, Naizhen Wu, Katsuki Takashima, Jungoh Ishimura, Hiroyuki Morita, Takuya Ito, Takeshi Kodama, Yuhei Yamasaki, Shin-ichi Akanuma, Yoshiyuki Kubo, Ken-ichi Hosoya, Hiroshi Tsuneki, Tsutomu Wada, Toshiyasu Sasaoka, Takahiro Shimizu, Hideki Sakai, Linda P. Dwoskin, Syed R. Hussaini, Ralph A. Saporito, Naoki Toyooka
Summary: This study achieved the total synthesis of two poison frog alkaloids, ent-cis-195A and cis-211A, in 16 and 19 steps, respectively. Interestingly, the absolute configuration of the parent decahydroquinoline nuclei of cis-211A was found to be the mirror image of that of cis-195A, despite both alkaloids being isolated from the same poison frog species.
Review
Endocrinology & Metabolism
Yasuhiro Onogi, Siegfried Ussar
Summary: Brown adipose tissue (BAT) is a tightly controlled regulator of body temperature and nutrient balance, influenced by complex hormonal and nervous inputs. This article provides an overview of key regulatory circuits, including diurnal rhythms and the interdependency of nutrient utilization in thermogenesis. It also discusses additional factors mediating sympathetic BAT activation and limitations of glucose-based BAT activity measurements.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Nutrition & Dietetics
Elena Lopez-Gonzales, Lisa Lehmann, Francisco Javier Ruiz-Ojeda, Rene Hernandez-Bautista, Irem Altun, Yasuhiro Onogi, Ahmed Elagamy Khalil, Xue Liu, Andreas Israel, Siegfried Ussar
Summary: Weight regain after fasting is a common issue in obesity treatment. Supplementing with L-serine can reduce weight regain and increase energy expenditure, which addresses a major problem in current obesity therapies.
Article
Endocrinology & Metabolism
Jiefu Wang, Yasuhiro Onogi, Martin Krueger, Josef Oeckl, Ruth Karlina, Inderjeet Singh, Stefanie M. Hauck, Regina Feederle, Yongguo Li, Siegfried Ussar
Summary: PAT2 serves as an important sensor of extracellular amino acids and regulates lysosomal acidification in brown adipocytes. Loss or overexpression of PAT2 affects lysosomal acidification and starvation-induced S6K re-phosphorylation, as PAT2 facilitates the assembly of lysosomal vATPase by recruiting the cytoplasmic V1 subunit to the lysosome.
MOLECULAR METABOLISM
(2022)
Article
Cell Biology
Hiroshi Tsuneki, Takahiro Maeda, Shinjiro Takata, Masanori Sugiyama, Koyuki Otsuka, Hinako Ishizuka, Yasuhiro Onogi, Emi Tokai, Chiaki Koshida, Kanta Kon, Ichiro Takasaki, Takeru Hamashima, Masakiyo Sasahara, Assaf Rudich, Daisuke Koya, Takeshi Sakurai, Masashi Yanagisawa, Akihiro Yamanaka, Tsutomu Wada, Toshiyasu Sasaoka
Summary: This study found that hypothalamic orexin is a key factor in preventing NASH and associated HCC under obesity. Orexin-deficient mice fed a high-fat diet showed severe obesity and progression of NASH with fibrosis in the liver. The study also revealed that long-term HFD feeding caused hepatocellular carcinoma (HCC) in orexin-deficient mice.
Article
Nutrition & Dietetics
Tsutomu Wada, Yuichiro Miyazawa, Misa Ikurumi, Kento Fuse, Akira Okekawa, Yasuhiro Onogi, Shigeru Saito, Hiroshi Tsuneki, Toshiyasu Sasaoka
Summary: A transdermal treatment with MC903 improves metabolism and reduces obesity in mice by increasing myofiber diameter, enhancing energy metabolism, and reducing visceral fat accumulation. It also suppresses adipogenic differentiation and promotes lipolysis in adipocytes.
NUTRITION & METABOLISM
(2023)
Article
Endocrinology & Metabolism
Hiroshi Tsuneki, Masanori Sugiyama, Toshihiro Ito, Kiyofumi Sato, Hiroki Matsuda, Kengo Onishi, Koharu Yubune, Yukina Matsuoka, Sanaka Nagai, Towa Yamagishi, Takahiro Maeda, Kosuke Honda, Akira Okekawa, Shiro Watanabe, Keisuke Yaku, Daisuke Okuzaki, Ryota Otsubo, Masanori Nomoto, Kaoru Inokuchi, Takashi Nakagawa, Tsutomu Wada, Teruhito Yasui, Toshiyasu Sasaoka
Summary: Food-odor perception can increase serum free fatty acids during fasting through olfactory memory and the central melanocortin and sympathetic nervous systems. Prior stimulation with food odor enhances whole-body lipid utilization, alters the sensitivity of the central agouti-related peptide system and sympathetic activity, and induces metabolic changes in peripheral tissues. Intermittent fasting combined with food-odor stimulation improves glycemic control and prevents insulin resistance in diet-induced obese mice.
Article
Endocrinology & Metabolism
Hisae Honoki, Kunimasa Yagi, Sayaka Tsuda, Tsutomu Wada, Asako Enkaku, Maki Nakagawa-Yokoyama, Miki Kamigishi, Masataka Shikata, Akiko Takikawa-Nishiday, Shiho Fujisaka, Daisuke Chujo, Toshiyasu Sasaoka, Akitoshi Nakashima, Shigeru Saito, Kazuyuki Tobe
Summary: The study presented a case of a Brazilian woman with type 1 diabetes who successfully gave birth after multiple miscarriages following the introduction of SAP-PLGS. By reducing glycemic fluctuations, this technology helps decrease hypoglycemic events and improve the success rate of pregnancy for women with diabetes.
DIABETOLOGY INTERNATIONAL
(2021)