Article
Biochemistry & Molecular Biology
Wangjuan Dai, Xiaofei Zou, Hongyan Jia, Yawen Peng, Boya La, Zhenjie Yan, Li Gao, Lianju Qin, Feiyang Diao, Xiang Ma, Yan Meng, Yugui Cui, Jiayin Liu
Summary: This study found that Prdx4 released by cumulus cells can repair meiotic defects in mouse oocytes and improve oocyte quality. Co-culturing immature oocytes with cumulus cells decreased oxidative stress and ER stress, leading to enhanced oocyte maturation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Masahide Konno, Yuri Sakata, Taku Ozaki
Summary: The present study demonstrates that the activation of mitochondrial calpain-5 triggers the truncation of caspase-4, suggesting that mitochondrial calpain-5 regulates the downstream pathway of caspase-4, including cell death and the inflammatory cascade. These findings provide new insights into ER-stress-related diseases such as Alzheimer's disease and cancer.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Shouji Matsushima, Junichi Sadoshima
Summary: Oxidative stress plays a critical role in the pathophysiology of myocardial ischemic-reperfusion (I/R) injury. NADPH oxidase (Nox) 2 and 4, major sources of reactive oxygen species (ROS) in cardiomyocytes, are upregulated in response to I/R. Suppression of Nox-derived ROS can prevent mitochondrial dysfunction and endoplasmic reticulum (ER) stress, leading to reduced myocardial I/R injury. However, minimal levels of ROS by either Nox2 or Nox4 are required for energy metabolism during I/R in order to preserve the levels of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and peroxisome proliferator-activated receptor-alpha (PPAR alpha). Furthermore, extreme suppression of Nox activity may lead to reductive stress and paradoxical increases in ROS levels. Nox4 has different roles in organelles such as mitochondria, ER, and ER-mitochondria contact sites (MAMs). Mitochondrial Nox4 has a detrimental effect, causing ROS-induced mitochondrial dysfunction during I/R, whereas Nox4 in the ER and MAMs may potentially protect against I/R injury through regulation of autophagy and MAM function, respectively. Although Nox isoforms are potential therapeutic targets for I/R injury, it is important to optimize ROS levels and selectively inhibit Nox4 in mitochondria to maximize the effect of intervention.
Article
Pharmacology & Pharmacy
Mei Ying Xuan, Shang Guo Piao, Jun Ding, Qi Yan Nan, Mei Hua Piao, Yu Ji Jiang, Hai Lan Zheng, Ji Zhe Jin, Can Li
Summary: Dapagliflozin alleviates renal fibrosis induced by unilateral ureteral obstruction (UUO) by inhibiting RIP1-RIP3-MLKL-mediated necroinflammation through Wnt3 alpha/beta-catenin/GSK-3 beta signaling.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Xin Li, Jiang Zhu, Qian Lin, Minjie Yu, Jianjun Lu, Jie Feng, Caihong Hu
Summary: This study found that curcumin has a protective effect, improving jejunal morphology and barrier function, enhancing mitochondrial function and ultrastructure, alleviating endoplasmic reticulum stress, and preventing excessive MAM formation and disorder.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Chongshan Dai, Subhajit Das Gupta, Zhanhui Wang, Haiyang Jiang, Tony Velkov, Jianzhong Shen
Summary: In this review, the authors summarized the current knowledge and challenges related to T-2 toxin-induced cardiotoxicity, and discussed potential treatment approaches. The cardiotoxicity caused by T-2 toxin involves various cellular pathways and mechanisms, including oxidative stress, mitochondrial dysfunction, and inflammatory responses. Antioxidant supplementation, induction of autophagy, and blockade of inflammatory signaling may provide protective effects against T-2 toxin-induced cardiac toxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Chemistry, Medicinal
Shijia Su, Jiarui Wang, Jing Wang, Ruili Yu, Likang Sun, Yue Zhang, Lei Song, Weiling Pu, Yi Tang, Yingli Yu, Kun Zhou
Summary: In this study, it was found that GP-17 can prevent myocardial ischemia/reperfusion injury by inhibiting endoplasmic reticulum stress, reducing cell apoptosis, autophagy, and oxidative stress. GP-17 also regulates the balance of mitochondrial fusion and fission, protecting mitochondrial function and improving cardiac function.
PHYTOTHERAPY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Minu Sharma, Amarjit S. Naura, S. K. Singla
Summary: Hyperoxaluria induces ER stress, leading to mitochondrial dysfunction possibly through alteration in sigma-1 receptor protein in the mitochondria-associated ER membranes, resulting in apoptosis, renal injury, and calcium oxalate crystal deposition.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Meijuan Meng, Xu Zhao, Ran Huo, Xuerui Li, Guangjun Chang, Xiangzhen Shen
Summary: The supplementation of disodium fumarate reduced rumen acidosis and improved the health of mammary gland tissue in Hu sheep. This was achieved by increasing rumen pH, reducing LPS concentration, decreasing calcium content in mammary gland tissue, and alleviating ER stress, mitochondrial damage, and oxidative stress.
Article
Immunology
Qinglong Guo, Jing Bi, Honghai Wang, Xuelian Zhang
Summary: EsxA, secreted by the ESX-1 secretion system, is a major virulence factor of Mycobacterium tuberculosis. EspC, an ESX-1 substrate, triggers ER stress-mediated apoptosis in macrophages, leading to inflammation, Ca2+ release, and bacterial survival. Understanding the role of EspC in Mtb-host interactions sheds light on complex host-pathogen interactions and mechanisms underlying pathogenesis mediated by the ESX-1 system.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Biochemistry & Molecular Biology
Ming Yuan, Mengqi Gong, Jinli He, Bingxin Xie, Zhiwei Zhang, Lei Meng, Gary Tse, Yungang Zhao, Qiankun Bao, Yue Zhang, Meng Yuan, Xing Liu, Cunjin Luo, Feng Wang, Guangping Li, Tong Liu
Summary: This study explores the potential role of ER stress-mitochondrial oxidative stress in diabetic atrial remodeling and AF induction. The results suggest that the IP3R1-GRP75-VDAC1 complex mediates ER stress-mitochondrial oxidative stress and plays an important role in diabetic atrial remodeling.
Review
Medicine, Research & Experimental
Smriti Verma, Bidya Dhar Sahu, Madhav Nilakanth Mugale
Summary: Hepatocellular carcinoma (HCC) is a deadly form of primary liver cancer, and the molecular mechanisms underlying its pathogenesis are poorly understood. Long non-coding RNAs (lncRNAs) play a crucial role in HCC, promoting tumor cell autophagy, proliferation, and migration by targeting and modulating downstream genes in cancer-related signaling pathways. Understanding lncRNAs may lead to improved knowledge of HCC etiology.
Review
Biochemistry & Molecular Biology
Yan Zhou, Dharmani Devi Murugan, Haroon Khan, Yu Huang, Wai San Cheang
Summary: ER stress is closely associated with the pathogenesis of diabetes and obesity, with excess nutrients and inflammatory cytokines as triggers. It not only induces endothelial dysfunction and atherosclerosis, but also contributes to oxidative stress.
Article
Environmental Sciences
Lin Li, Rong-Jie Jin, Lu Ji
Summary: The effect of pachymic acid (PA) on pulmonary fibrosis in rats was investigated. PA was found to alleviate pulmonary edema, fibrosis, and histopathological damage. It achieved this by inhibiting ER stress and improving mitochondrial function.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Environmental Sciences
Junjun Peng, Xueyan Dai, Huiqin Fan, Chenghong Xing, Yu Zhuang, Xiaona Gao, Huabin Cao, Guoliang Hu, Fan Yang
Summary: Copper (Cu) at high levels can cause harm to the host physiology, specifically resulting in nephrotoxicity, although the exact mechanism remains unclear. This study explored the relationship between mitochondria-associated ER membranes (MAM) and Cu-induced kidney mitochondrial dysfunction and ER stress. The findings revealed that excessive Cu disrupted MAM integrity, leading to MAM dysfunction, mitochondrial dysfunction, and ER stress, contributing to Cu-induced nephrotoxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Paulo J. Oliveira
EUROPEAN HEART JOURNAL
(2022)
Editorial Material
Medicine, General & Internal
Anabela Marisa Azul, Martin Winter, Daniel Silva, Liljana Georgievska, Paulo J. Oliveira
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2022)
Review
Pharmacology & Pharmacy
Catarina Melim, Maria R. Lauro, Isabel M. Pires, Paulo J. Oliveira, Celia Cabral
Summary: The gastrointestinal tract, composed of rapidly renewing cells, is prone to cancer. Colorectal cancer, a commonly diagnosed GI cancer, lacks effective treatment. Therefore, exploring new therapeutic strategies involving biologically active phytocompounds is essential. Cruciferous vegetables, rich in various nutrients, particularly glucosinolates, have shown potential as anti-cancer agents. This article discusses the importance of proper compound formulations to enhance the stability and bioaccessibility of crucial compounds in Cruciferous vegetables for the prevention and treatment of GI cancers.
Article
Endocrinology & Metabolism
Aryane Cruz Oliveira Pinho, Diana Santos, Ines Baldeiras, Ana Burgeiro, Emelindo C. Leal, Eugenia Carvalho
Summary: Thoracic perivascular adipose tissue (tPVAT) exhibits characteristics similar to brown adipose tissue and is associated with vascular dysfunction. UCP1 expression is higher in tPVAT compared to inguinal white adipose tissue (iWAT), with significant changes in oxidative stress and mitochondrial respiration observed in diabetic mice.
JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carlos Fernandes, Fernando Cagide, Jorge Simoes, Carlos Pita, Eurico Pereira, Afonso J. C. Videira, Pedro Soares, Jose F. S. Duarte, Antonio M. S. Santos, Paulo J. Oliveira, Fernanda Borges, Filomena S. G. Silva
Summary: Targeting mitochondria with antioxidants is a promising strategy to prevent cellular senescence and skin ageing. This study investigates the potential use of mitochondria-targeted antioxidants (MitoBENs) as active ingredients to prevent senescence in skin cells. Among the tested compounds, the pyrogallol derivative MB2 showed the least toxicity and had a potent antioxidant protective effect against hydrogen peroxide cytotoxicity. It also decreased transcripts related to senescence in a 3D skin cell model. These findings suggest that MB2 could be a good candidate for further development as an active ingredient in skin anti-ageing products.
Article
Chemistry, Medicinal
Sofia Benfeito, Carlos Fernandes, Daniel Chavarria, Sandra Barreiro, Fernando Cagide, Lisa Sequeira, Jose Teixeira, Renata Silva, Fernando Remiao, Paulo J. Oliveira, Eugenio Uriarte, Fernanda Borges
Summary: Although TPP+ cation is commonly used to target antioxidants to mitochondria, it has shown cytotoxicity in vitro. This study explored nitrogen-derived cationic carriers as alternative compounds to TPP. The results showed that these nitrogen-based cationic carriers were not cytotoxic and exhibited neuroprotective effects against oxidative insults.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Ermelindo C. Leal, Tatiana Emanuelli, Diana Santos, Joao Moura, Ana Catarina Rg Fonseca, Ana Burgeiro, Eugenia Carvalho
Summary: This study investigated the impact of alterations in the unfolded protein response (UPR), autophagy, and mitochondrial function on wound healing (WH) under diabetic conditions. The findings showed that the UPR and autophagy pathways were activated in the skin of control mice and non-wounded skin of diabetic mice. Additionally, high-glucose conditions induced autophagy in keratinocyte and fibroblast cell cultures. However, there were no changes in mitophagy in the skin of diabetic mice or wounded skin.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2023)
Article
Environmental Sciences
Jelena Stevanovic-Silva, Jorge Beleza, Pedro Coxito, Paulo J. Oliveira, Antonio Ascensao, Jose Magalhaes
Summary: Maternal high-caloric nutrition and gestational diabetes mellitus (GDM) increase the risk of liver metabolic alterations in mothers and offspring. Exercise is recommended in GDM treatment. This study showed that gestational exercise protected mothers from diet-induced GDM metabolic consequences and ameliorated liver mitochondrial alterations in offspring.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Ines Ramos Rego, Daniela Silverio, Maria Isabel Eufrasio, Sandra Sofia Pinhancos, Bruna Lopes da Costa, Jose Teixeira, Hugo Fernandes, Yang Kong, Yao Li, Stephen H. Tsang, Paulo J. Oliveira, Rosa Fernandes, Peter M. J. Quinn, Paulo Fernando Santos, Antonio Francisco Ambrosio, Celso Henrique Alves
Summary: Age-related macular degeneration (AMD) is a major cause of visual impairment and blindness in the elderly. The damage to retinal pigment epithelium (RPE) caused by oxidative stress is a key factor in the development of AMD, and this damage is caused by the accumulation of reactive oxygen species (ROS) primarily produced by mitochondria. The protein TRAP1, found in mitochondria, helps maintain mitochondrial integrity and reduces ROS production. This study found that TRAP1 is present in human RPE cells and its levels decrease when exposed to hydrogen peroxide. Silencing TRAP1 leads to increased ROS production and decreased mitochondrial respiratory capacity in RPE cells.
Article
Nutrition & Dietetics
Jose P. Castelao-Baptista, Sara A. Valente, Sara Canario, David Oppolzer, Ana Barros, Carlos Venancio, Tania Martins, Luis Antunes, Vilma A. Sardao, Eduardo Rosa, Paulo J. Oliveira
Summary: Obesity, a rising concern in the Eastern world, is often accompanied by non-alcoholic fatty liver disease (NAFLD). Researchers are investigating potential natural interventions to reduce obesity complications. This study focuses on Brassica by-products (BBPs) as an example of edible plant waste and examines the effects of different extracts from broccoli by-products on an in vitro model of fatty acid-induced liver toxicity. The extracts showed a dose-dependent decrease in cell viability of HepG2 cells, but did not compromise mitochondrial function or lipid accumulation. They effectively reduced lipid accumulation induced by fatty acids in HepG2 cells. The extracts' ability to prevent decrease in catalase activity may explain the observed effects.
Article
Nutrition & Dietetics
Diana Sousa, Mariana Rocha, Andreia Amaro, Marcos Divino Ferreira-Junior, Keilah Valeria Naves Cavalcante, Tamaeh Monteiro-Alfredo, Catia Barra, Daniela Rosendo-Silva, Lucas Paulo Jacinto Saavedra, Jose Magalhaes, Armando Caseiro, Paulo Cezar de Freitas Mathias, Susana P. Pereira, Paulo J. Oliveira, Rodrigo Mello Gomes, Paulo Matafome
Summary: Obesogenic environments during the perinatal period can reprogram offspring energy balance mechanisms and increase the risk of metabolic diseases in adulthood. Different obesogenic models have varying effects on metabolic parameters and energy storage pathways in offspring.
Review
Chemistry, Medicinal
Mario P. Marques, Laura Mendonca, Beatriz G. Neves, Carla Varela, Paulo Oliveira, Celia Cabral
Summary: This review investigates the traditional applications of Lamiaceae medicinal plants used by local communities in the Iberian Peninsula for wound healing. The information from Iberian ethnobotanical surveys is summarized, and the pharmacological evidence for eight out of twenty-nine Lamiaceae species is presented. Future studies should focus on isolating and identifying the active molecules of these plants, followed by robust clinical trials to confirm their safety and effectiveness in wound healing.
Article
Medicine, Research & Experimental
Luis F. Grilo, Joao D. Martins, Mariana S. Diniz, Carolina Tocantins, Chiara H. Cavallaro, Ines Baldeiras, Teresa Cunha-Oliveira, Stephen Ford, Peter W. Nathanielsz, Paulo J. Oliveira, Susana P. Pereira
Summary: Maternal obesity may significantly affect liver function and pathophysiology, increasing the risk of pregnancy-associated liver diseases and hepatic diseases. Oxidative stress and mitochondrial dysfunction may play crucial roles in this process.
Article
Environmental Sciences
Elsa T. Rodrigues, Eduarda Pereira, Paulo J. Oliveira, Miguel A. Pardal
Summary: The present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. It also highlights the capability of this assay to discriminate influent and effluent toxic characteristics and its suitability for water quality monitoring and surveillance of treatment processes.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Medicine, General & Internal
Aryane Cruz Oliveira Pinho, Diana Santos, Paulo J. Oliveira, Ermelindo Carreira Leal, Eugenia Carvalho
Summary: This study investigated the effects of diabetes on mitochondrial respiration in wounded and non-wounded skin using high-resolution respirometry. The results showed that the oxidative phosphorylation capacity of the wounded skin was decreased at day 3 post-wounding and increased at day 10 post-wounding in diabetic mice. The relative contribution of pyruvate, malate, and glutamate oxidation to the oxidative phosphorylation capacity was higher in wounded skin, while fatty acid oxidation contributed more to the non-wounded skin. This study provides important insights into the impact of diabetes on wound healing.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)