Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 677, Issue 1-3, Pages 154-162Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2011.12.020
Keywords
Asthma; Neutrophil; Tr1 cell; Chemokine; Inflammation; Eosinophil
Categories
Funding
- Japan Society for the Promotion of Science [23590093]
- Grants-in-Aid for Scientific Research [23590093] Funding Source: KAKEN
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It has been suggested that interleukin (IL)-10 exerts immunosuppressive effects on allergic inflammation, including asthma, mainly through inhibition of Th2 cell-mediated eosinophilic airway inflammation. In a model of experimental asthma utilizing multiple intratracheal antigen challenges in sensitized mice, IL-10 production as well as eosinophilia and neutrophilia in the lung were induced by the multiple challenges. In this study, we set out to reveal the cellular source of endogenously produced IL-10, and the roles of IL-10 in airway leukocyte inflammation using an anti-IL-10 receptor monoclonal antibody. Balb/c mice were sensitized i.p. with ovalbumin+ Al(OH)(3), and then challenged by intratracheal administration of ovalbumin 4 times. Flow cytometric analyses revealed that the cellular source of IL-10 was CD4(+) T cells lacking the transcription factor, forkhead box P3. Treatment with anti-IL-10 receptor monoclonal antibody prior to the 4th challenge significantly augmented airway neutrophilia as well as the production of IL-1 beta, and CXC chemokines, keratinocyte-derived chemokine (KC) and macrophage inflammatory protein (MIP)-2, but not airway eosinophilia, Th2 cytokine (IL-4 and IL-5) production, or a late-phase increase in specific airway resistance. Approximately 40% of IL-10 receptor(+) cells expressed the macrophage marker F4/80, whereas only 3-4% of the IL-10 receptor(+) cells were granulocyte differentiation antigen (Gr)-1(high) cells (neutrophils). In conclusion, multiple airway antigen challenges induced the proliferation of IL-10-expressing CD4(+) T cells in regulating airway neutrophilia. Systemic blockade of IL-10 function coincided with increases in IL-1 beta and CXC chemokines. Thus, IL-1 beta and CXC chemokines may be targets for development of novel pharmacotherapy for neutrophilic asthma. (C) 2011 Elsevier B.V. All rights reserved.
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