Left ventricular enlargement in coxsackievirus-B3 induced chronic myocarditis - ongoing inflammation and an imbalance of the matrix degrading system

Enteroviruses, especially Coxsackie B3 virus (CVB-3), cause acute viral myocarditis, but the detailed mechanisms leading to chronic left ventricular dysfunction and dilatation remain elusive Myocardial tissues of CVB-3 infected and sham infected male swr/J mice were analyzed after hemodynamic evaluation on clays 4, 7, and 28 p i by RT-PCR, gelatin zymography, ELISA. immunohistochemistry, sinus red staining, and luxol fast blue staining In the early phase after infection an abnormal diastolic function was the main hemodynamic finding CVB-3 infection caused impairment of left ventricular function combined with ventricular dilatation 7 and 28 days post-infection These hemodynamic findings were associated with relevant upregulation of different cytokines (IL-1 beta, IL-6, IL-10, INF-gamma, and TNF-alpha) in the acute phase with persistent over-expression of IL-6, IL-10, and INF-gamma in the chronic phase This virus induced myocardial inflammation was linked to a significant induced MMP/TIMP system (MMP-2,-3,-8. TIMP-1, uPA, tPA-mRNA expression. and MMP-2-activity) in the acute and chronic phase leading to imbalance in the MMP/TIMP-ratio at clay 28 This imbalance in the MMP/TIMP system was significantly correlated to the development of ventricular dilatation. Viral persistence induces chronic myocardial inflammation and ail imbalance of the matrix degrading system, associated with the development of left ventricular dysfunction and dilatation in chronic murine myocarditis (C) 2010 Elsevier B.V All rights reserved