Article
Microbiology
Wai-Yin Lui, Aradhana Bharti, Nok-Hei Mickey Wong, Sonia Jangra, Michael Botelho, Kit-San Yuen, Dong-Yan Jin
Summary: This study identifies BPLF1 as a virulence factor of EBV that evades human innate immune responses by removing polyubiquitin marks on critical effector proteins. The study demonstrates the importance of the DUB activity of BPLF1 in suppressing antiviral responses and provides insights into the pathogenesis of EBV and potential antiviral strategies.
Article
Immunology
Hangye Zhang, Feihu Shen, Jiali Yu, Jieyun Ge, Yifan Sun, Haitian Fu, Yang Cheng
Summary: In this study, the role of Plasmodium proteins on collagen in human splenic fibroblasts (HSFs) was reported and verified for the first time. The protein PvTRAg23 specifically reduced the level of collagen I in HSFs and activated the NF-kappa Bp65 pathway to produce inflammatory cytokines, leading to collagen alterations. This study contributes to a better understanding of structural changes and immune responses in the host spleen after Plasmodium infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Mahmoud Darweesh, Shady Younis, Zamaneh Hajikhezri, Arwa Ali, Chuan Jin, Tanel Punga, Soham Gupta, Magnus Essand, Leif Andersson, Goran Akusjarvi
Summary: ZC3H11A protein is involved in the regulation of NF-κB signaling pathway, and its depletion leads to enhanced signaling and accumulation defects of IκBα mRNA and protein.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Huiyuan Zhu, Qianyu Li, Qiongyi Huang, Huiqiong Yang, Jiayi Zheng, Ruting Xie, Dongyan Han, Qing Wei
Summary: RIG-I plays a crucial role in promoting epithelial keratinocyte proliferation and wound repair by inducing TIMP-1 expression via the NF-κB signaling pathway. TIMP-1 directly accelerates keratinocyte proliferation and promotes wound healing, making RIG-I a potential biomarker for assessing skin injury severity and a therapeutic target for chronic wounds such as diabetic foot.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Chemistry, Analytical
Kanyu Xun, Yue Sun, Qiang Zhang, Nachuan Wen, Zhimin Wang, Liping Qiu, Weihong Tan
Summary: This study developed a new nanoplatform using streptavidin as a carrier to directly analyze and manipulate the activity of target proteins. By incorporating a photoresponsive module, the nanoplatform was able to control the nuclear translocation behavior of active proteins and regulate downstream signaling events.
ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Pooja Gupta, Ananda Guha Majumdar, Birija Sankar Patro
Summary: This study identifies a non-enzymatic role of Werner RECQL helicase in the processing of TOP1cc and subsequent NF-kappa B activation, offering a potential targeted therapy for cancer patients with WRN deficiency.
Article
Immunology
Lei Wang, Pengfei Li, Yu Zhou, Renjun Gu, Ge Lu, Chunbing Zhang
Summary: This study investigated the effects of Magnoflorine (Mag) on rheumatoid arthritis (RA) and the underlying mechanism using mouse models. The results showed that Mag could reduce arthritis severity, joint destruction, and macrophage infiltration. Further analysis revealed that Mag exerted its anti-arthritic and anti-inflammatory effects by inhibiting the activation of NF-kappa B and MAPK signaling pathways.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Review
Oncology
Run Shi, Zhe Zhang, Ankai Zhu, Xingxing Xiong, Jie Zhang, Jiang Xu, Man-Sun Sy, Chaoyang Li
Summary: Collagen, the most abundant protein in animals, plays a crucial role in tumor development. Specifically, type I collagen, which is upregulated during tumorigenesis, promotes tumor cell proliferation, epithelial-mesenchymal transition, metastasis, and affects the efficacy of tumor therapies. However, the roles of type I collagen in tumor biology are complex and depend on the tumor type. This review discusses the expression and regulation of type I collagen synthesis, its role in cancer progression, and recent approaches targeting type I collagen for cancer treatment.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Review
Endocrinology & Metabolism
Robert C. Baxter
Summary: The 6 high-affinity insulin-like growth factor binding proteins (IGFBPs) have diverse functions in cell signaling and can impact cell survival, migration, metabolism, and other cellular functions. They interact with multiple pathways and regulatory proteins, including integrins and transforming growth factor beta family receptors. IGFBPs can also regulate nuclear hormone receptors, senescence, DNA damage repair, and immune functions. Understanding the complexity of IGFBPs' interactions may provide therapeutic opportunities for modulating IGFBP-dependent signaling.
Review
Biochemistry & Molecular Biology
Li Tao, Xiaomeng Ren, Wenhui Zhai, Zheng Chen
Summary: The non-canonical NF-kappa B signaling pathway plays a crucial role in liver diseases by regulating liver homeostasis and participating in various pathological processes. Small-molecule inhibitors targeting this pathway have shown potential in the treatment of liver injuries.
Article
Microbiology
Yi-Sheng Kao, Li-Chiu Wang, Po-Chun Chang, Heng-Ming Lin, Yee-Shin Lin, Chia-Yi Yu, Chien-Chin A. Chen, Chiou-Feng Lin, Trai-Ming A. Yeh, Shu-Wen Wan, Jen-Ren A. Wang, Tzong-Shiann Ho, Chien-Chou A. Chu, Bo-Cheng Zhang, Chih-Peng A. Chang
Summary: A study reveals that integrin-linked kinase (ILK) enhances dengue virus (DENV) infection by activating the Akt-Erk-NF-kappa B pathway and inducing the expression of SOCS3, which inhibits interferon-stimulated gene expression. Inhibiting ILK reduces DENV production in cells and decreases viral loads in mouse brains, as well as mortality. ILK is, therefore, a potential therapeutic target for DENV infection.
Article
Biochemistry & Molecular Biology
Giovanna M. Bernal, Longtao Wu, David J. Voce, Ralph R. Weichselbaum, Bakhtiar Yamini
Summary: This study found that the p52 subunit increases in the nucleus of cells during senescence, and factors in the conditioned media play a role in promoting p52 nuclear translocation. Additionally, mature p52 can induce cellular senescence. These findings support the observation of increased p52 in aged tissue and suggest that p52 contributes to organismal aging.
CELL AND BIOSCIENCE
(2022)
Review
Immunology
Zhending Gan, Meiyu Zhang, Donghui Xie, Xiaoyan Wu, Changming Hong, Jian Fu, Lijuan Fan, Shengyi Wang, Sufang Han
Summary: Glycine, acting as an inhibitory neurotransmitter, not only affects the functions of macrophages but also shapes their polarization through cellular signaling pathways and microRNAs. Additionally, glycine has beneficial effects in preventing and treating macrophage-associated diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Dong Young Kang, Nipin Sp, Eun Seong Jo, Jin-Moo Lee, Kyoung-Jin Jang
Summary: The study investigated the role of iron/heme metabolism in induced inflammatory conditions, indicating that iron and heme formation and synthesis play a crucial role in enhancing inflammatory responses. It was also found that inhibiting heme synthesis or the TLR4/NF-kappa B signaling pathway can effectively suppress inflammatory responses.
Article
Immunology
Bo Wu, Lihua Qiang, Yong Zhang, Yesheng Fu, Mengyuan Zhao, Zehui Lei, Zhe Lu, Yan-Ge Wei, Hongmiao Dai, Yingwei Ge, Mingqiu Liu, Xuemei Zhou, Zhiqiang Peng, Hongchang Li, Chun-Ping Cui, Jing Wang, Hui Zheng, Cui Hua Liu, Lingqiang Zhang
Summary: The E3 ubiquitin ligase (E3)-mediated ubiquitination and deubiquitinase (DUB)-mediated deubiquitination play crucial roles in colonic inflammation. Ovarian tumor deubiquitinase 1 (OTUD1) inhibits RIPK1-mediated NF-kappa B activation to alleviate inflammation, with lower expression in UC patients. OTUD1 is a potential target for treating IBD due to its regulatory role in intestinal inflammation.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Adam Kazberuk, Magda Chalecka, Jerzy Palka, Arkadiusz Surazynski
Summary: Nonsteroidal anti-inflammatory drugs (NSAIDs) may induce apoptosis through the activation of PPAR gamma and the proline metabolism enzyme PRODH/POX in breast cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Adam Kazberuk, Magda Chalecka, Jerzy Palka, Katarzyna Bielawska, Arkadiusz Surazynski
Summary: This study found that non-steroidal anti-inflammatory drugs (NSAIDs) induce apoptosis in breast cancer cells through the activation of peroxisome proliferator-activated receptor-gamma (PPAR gamma) and the generation of reactive oxygen species (ROS) via proline dehydrogenase/proline oxidase (PRODH/POX). The effect of NSAIDs on cell apoptosis is independent of cyclooxygenase-2 (COX-2) inhibition. Additionally, the study showed that the role of PRODH/POX in NSAID-induced apoptosis is more pronounced in breast cancer cells with PRODH/POX knockout. The findings suggest a potential therapeutic strategy for cancer treatment using NSAIDs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Olga Klaudia Szewczyk, Piotr Roszczenko, Robert Czarnomysy, Anna Bielawska, Krzysztof Bielawski
Summary: Nanotechnology shows great potential in advanced cancer therapies, allowing for simultaneous drug administration and immune system engagement. Nanoparticles can locate metastases and deliver medications, effectively reducing tumors with minimal toxicity to healthy tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Karolina Lendzion, Agnieszka Gornowicz, Jakub W. Strawa, Katarzyna Bielawska, Robert Czarnomysy, Bozena Poplawska, Krzysztof Bielawski, Michal Tomczyk, Wojciech Miltyk, Anna Bielawska
Summary: This study evaluated the composition and biological activities of oil, extracts, and fractions obtained from S. hispanica seeds, suggesting their potential use for breast cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sara Janowska, Dmytro Khylyuk, Agnieszka Gornowicz, Anna Bielawska, Michal Janowski, Robert Czarnomysy, Krzysztof Bielawski, Monika Wujec
Summary: Based on previous work, we designed and synthesized 1,3,4-thiadiazole derivatives and evaluated their cytotoxic activity against breast cancer cells. The compounds displayed weak anticancer activity, with SCT-4 showing the most significant effect on MCF-7 breast cancer cells. Further investigation suggested a possible multitarget mode of action for the synthesized compounds, with a potential involvement of caspase 8.
Article
Biochemistry & Molecular Biology
Iryna Ivasechko, Ihor Yushyn, Piotr Roszczenko, Julia Senkiv, Nataliya Finiuk, Danylo Lesyk, Serhii Holota, Robert Czarnomysy, Olga Klyuchivska, Dmytro Khyluk, Nataliya Kashchak, Andrzej Gzella, Krzysztof Bielawski, Anna Bielawska, Rostyslav Stoika, Roman Lesyk
Summary: Novel pyridine-thiazole hybrid molecules showed high antiproliferative activity against various cancer cell lines, suggesting their potential as anticancer agents. The selective cytotoxicity of these compounds prompted further investigation into their mechanisms of action, which may be related to inducing genetic instability in tumor cells.
Article
Oncology
Dominika Radomska, Robert Czarnomysy, Anna Szymanowska, Dominik Radomski, Enrique Dominguez-Alvarez, Anna Bielawska, Krzysztof Bielawski
Summary: This study evaluated the anticancer activity of novel selenoesters in breast cancer cells, and the results showed that these compounds exhibited high cytotoxicity, induced apoptosis and autophagy, and hindered the cell cycle. These findings suggest that selenium-containing compounds have significant potential as candidates for anticancer agents.
Article
Biochemistry & Molecular Biology
Przemyslaw Wielgat, Karol Rogowski, Robert Czarnomysy, Natalia Wawrusiewicz-Kurylonek, Karolina Narejko, Krzysztof Bielawski, Halina Car
Summary: This study investigated the effect of tamoxifen on the sialic acid-Siglec interplay in breast cancer and its significance in immune conversion. The results showed changes in cytokine profiles and immune phenotype switching, as well as alterations in SIGLEC5 and SIGLEC14 genes and their products in response to tamoxifen. Moreover, tamoxifen increased the binding of Siglec-5 and Siglec-14 fusion proteins to breast cancer cells, independent of estrogen dependency. These findings suggest the potential use of Siglec expression profiles in breast cancer patients for therapeutic strategies and predicting patient outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Agnieszka Gornowicz, Roman Lesyk, Robert Czarnomysy, Serhii Holota, Yulia Shepeta, Bozena Poplawska, Magdalena Podolak, Wojciech Szymanowski, Krzysztof Bielawski, Anna Bielawska
Summary: Combining chemotherapy with immunotherapy is a promising strategy in treating gastric cancer with confirmed HER2 expression. Novel 4-thiazolidinone derivatives and anti-HER2 antibodies show potential in increasing the sensitivity of gastric cancer cells to treatment. The molecular mechanism of action involves apoptosis induction and decreased inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Olga Michalak, Marcin Cybulski, Wojciech Szymanowski, Agnieszka Gornowicz, Marek Kubiszewski, Kinga Ostrowska, Piotr Krzeczynski, Krzysztof Bielawski, Bartosz Trzaskowski, Anna Bielawska
Summary: A series of new derivatives of ursolic acid (UA) were designed and synthesized by substituting various amino acids (AAs) or dipeptides (DP) at the C-3 position of the steroid skeleton. These conjugates exhibited cytotoxic activity against hormone-dependent and triple-negative breast cancer cells. Mechanistic studies revealed the activation of apoptotic pathways for some compounds and the induction of autophagy for others. Additionally, the synthesized compounds showed potential as anticancer agents based on their ADME properties and molecular docking to the estrogen receptor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Pawel Misiak, Katarzyna Niemirowicz-Laskowska, Karolina H. Markiewicz, Przemyslaw Wielgat, Izabela Kurowska, Robert Czarnomysy, Iwona Misztalewska-Turkowicz, Halina Car, Krzysztof Bielawski, Agnieszka Z. Wilczewska
Summary: The research focuses on synthesizing polymer nanoparticles (PNPs) for doxorubicin delivery. Various block and statistical copolymers with ketoester derivative, N-isopropylacrylamide, and cholesterol were prepared. The doxorubicin-loaded PNPs demonstrated high efficacy against estrogen-dependent MCF-7 breast cancer cell lines at low doses and showed good compatibility with normal cells. The study confirms the impact of PNPs on membrane degradation, accumulation of reactive oxygen species (ROS), and cell cycle arrest, all of which are closely related to cell death.
CANCER NANOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Emilia Szymanska, Joanna Potas, Marcin Baranowski, Robert Czarnomysy, Magdalena Ewa Sulewska, Anna Basa, Malgorzata Pietruska, Krzysztof Bielawski, Katarzyna Winnicka
Summary: Emulgels composed of natural polysaccharide gums (tragacanth, xanthan, and gellan) were evaluated as oromucosal platforms of delta-aminolevulinic acid (ALA) for photodynamic therapy (PDT). These emulgels demonstrated superior mucoadhesiveness and textural properties compared to commercial oromucosal gels. Furthermore, they promoted the absorption rate of ALA and enhanced photosensitizer retention in the buccal epithelium.