Article
Biology
Peter R. Strege, Luke M. Cowan, Constanza Alcaino, Amelia Mazzone, Christopher A. Ahern, Lorin S. Milescu, Gianrico Farrugia, Arthur Beyder
Summary: Shear stress and patch suction can reversibly alter the kinetic properties and maximum current of NaChBac, suggesting it is mechanosensitive. Structural analysis reveals a large displacement of the intracellular gate, supporting the proposed mechanosensitive mechanism.
Article
Neurosciences
John Ridley, Sam Manyweathers, Raymond Tang, Tom Goetze, Nadine Becker, Ilka Rinke-Weiss, Robert Kirby, Alison Obergrussberger, Marc Rogers
Summary: This study utilized hASIC1a as a case study to develop and validate low, medium, and high throughput automated patch clamp (APC) assays for screening and mechanistic profiling of new ligands. By optimizing single and stacked pipette tip applications available on each APC platform, stable pH-evoked currents during multiple ligand applications enabled cumulative EC50 and IC50 determinations with minimized receptor desensitization.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Review
Pharmacology & Pharmacy
Fritz Markwardt
Summary: Patch clamp investigations help understand the molecular function of single ion channels. The human P2X7 receptor is an ATP-gated ion channel involved in inflammatory and nociceptive reactions. Specific residues and domains play crucial roles in ion channel function.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biology
Jan L. Muench, Fabian Paul, Ralf Schmauder, Klaus Benndorf
Summary: Inferring appropriate kinetic models for ion channel gating from ensemble currents is a challenging task due to limited data. We propose a Bayesian filter approach that specifies hidden Markov models for current and fluorescence data, which shows flexibility and superior performance compared to traditional methods. The approach can handle different noise distributions and incorporate orthogonal fluorescence data, providing more accurate parameter estimates.
Article
Biochemistry & Molecular Biology
Karl Josef Foehr, Michael Rapp, Michael Fauler, Thomas Zimmer, Bettina Jungwirth, David Alexander Christian Messerer
Summary: Aripiprazole has been identified as a potent blocker of the dominant voltage-gated sodium channel of heart muscle. Its interactions with the channel differ depending on the state, with weaker affinity for the resting state and stronger affinity for the inactivated state.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Marta Martini, Giorgio Rispoli
Summary: Some sensory disorders in hearing, vestibular, and vision can be attributed to voltage-gated Ca2+ channels in sensory cells. In this study, the Na+ current flowing through L- and R-type Ca2+ channels in labyrinth hair cells was investigated. The activation and deactivation of the Na+ current in L-type channels were affected by low Ca2+ concentration, resulting in increased current amplitude. The R-type Na+ current was partially blocked by nifedipine and its current amplitude increased in low Ca2+ condition. Furthermore, the selectivity filter of R channels was less affected by external Ca2+ compared to L channels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Eva Korpos, Ferenc Papp
Summary: This study reports an unexpected expansion of the voltage-gated proton channel family. Through searching for proton channel candidates in the genome of Aplysia californica, three sequences named AcHv1, AcHv2, and AcHv3 were found. Electrophysiological experiments showed that AcHv1 and AcHv2 are voltage-gated channels, while AcHv3 constantly leaks protons.
Article
Cell & Tissue Engineering
Lingyun Li, Huan Zhao, Haohuan Xie, Tasneem Akhtar, Yichuan Yao, Yuan Cai, Kai Dong, Yonghao Gu, Jin Bao, Jutao Chen, Mei Zhang, Kai Zhong, Weiping Xu, Tian Xue
Summary: This study comprehensively characterized the physiological maturation of photoreceptor-like cells derived from hiPSCs in ROs, revealing progressive maturation of ion channels associated with photoreceptor function during differentiation. The inward-outward current ratio was identified as a sensitive indicator of photoreceptor maturity and pathological state, providing a potential biomarker for quality control during RO differentiation.
Article
Multidisciplinary Sciences
Shuo Han, Xiang-Ping Chu, Ryan Goodson, Prae Gamel, Sophia Peng, Joshua Vance, Shizhen Wang
Summary: Cholesterol inhibits the hHv1 channel by stabilizing the voltage-sensing S4 segment at resting conformations, providing a mechanism for the regulation of fertilization by the hHv1 channel.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Yu Yuan, Dawid Jaslan, Taufiq Rahman, Stephen R. Bolsover, Vikas Arige, Larry E. Wagner, Carla Abrahamian, Rachel Tang, Marco Keller, Jonas Hartmann, Anna S. Rosato, Eva-Maria Weiden, Franz Bracher, David Yule, Christian Grimm, Sandip Patel
Summary: This study reveals that TPC2 can selectively increase its permeability to calcium ions when co-activated by the endogenous ligands NAADP and PI(3,5)P-2. This finding indicates that the flux of different ions through the same channel can be independently controlled and identifies TPC2 as a potential coincidence detector that optimizes lysosomal calcium signaling.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Stefan Schwerin, Catharina Westphal, Claudia Klug, Gerhard Schneider, Matthias Kreuzer, Rainer Haseneder, Stephan Kratzer
Summary: Recent evidence suggests that dexmedetomidine might directly inhibit HCN channels, but the effects are modest at clinically relevant concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Young-Woo Nam, Meng Cui, Naglaa Salem El-Sayed, Razan Orfali, Misa Nguyen, Grace Yang, Mohammad Asikur Rahman, Judy Lee, Miao Zhang
Summary: The selectivity of CyPPA among K(Ca)2 and K(Ca)3.1 channel subtypes relies on the HA/HB helices.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell Biology
Adam Feher, Marianna Pocsi, Ferenc Papp, Tibor G. Szanto, Agota Csoti, Zsolt Fejes, Bela Nagy, Balazs Nemes, Zoltan Varga
Summary: This study identified multiple voltage-gated sodium channels in the plasma membrane of human B cells using electrophysiological and molecular biology methods. The detected sodium currents showed sensitivity between TTX-sensitive and TTX-insensitive channels, suggesting the co-existence of multiple Na(V)1 subtypes. The results also supported the functional role of these channels in shaping and maintaining the resting membrane potential.
Article
Multidisciplinary Sciences
Bingxue Li, Songling Li, Honglan Zheng, Zhiqiang Yan
Summary: Research shows that in Drosophila larvae, the MET currents in Cho neurons are mediated by two TRPV channels, NAN and IAV, with the C-terminal residues 857 through 990 of IAV regulating the kinetics of MET currents. Additionally, the NompC channel contributes to the adaptation of auditory transduction currents.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Pharmacology & Pharmacy
Shirin Ahmadi, Melisa Benard-Valle, Kim Boddum, Fernanda C. Cardoso, Glenn F. King, Andreas Hougaard Laustsen, Anne Ljungars
Summary: Ion channels are crucial for various physiological processes. Venomous creatures exploit ion channels by producing toxins that target them, leading to dysregulation which can cause severe complications in humans. However, the discovery of selective venom toxins has contributed to the development of drugs for envenoming and ion channel-related diseases.
FRONTIERS IN PHARMACOLOGY
(2023)