Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 581, Issue 3, Pages 235-243Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2007.11.060
Keywords
mu-opioid receptors; dopamine D-1 receptors; hetero-oligomerization; cell surface expression; nuclear localization signal
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Funding
- NIDA NIH HHS [R01 DA007223] Funding Source: Medline
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Our immunohistochemistry experiments demonstrated that the mu-opioid receptor co-localized with the dopamine D-1 receptor in neurons of the cortex and caudate nucleus. On the basis of this physiological data we further investigated whether these two G protein coupled receptors formed hetero-oligomers in living cells. To demonstrate hetero-oligomerization we used a novel strategy, the method used harnessed the physiological cellular mechanism for transport of proteins to the nucleus. The nuclear translocation pathway was adapted for the visualization of mu-opioid hetero-oligomers with the dopamine D-1 receptor. The receptor hetero-oligomer complex formed resulted in a significantly enhanced surface expression of mu-opioid receptor. This hetero-oligomer formation involved the interaction of mu-opioid receptor with the dopamine D-1 receptor carboxyl tail, since a dopamine D-1 receptor substituted with the carboxyl of the dopamine D-5 receptor failed to increase surface expression of mu-opioid receptor. (C) 2007 Elsevier B.V. All rights reserved.
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