Article
Biology
David B. Halling, Ashley E. Philpo, Richard W. Aldrich, Jon T. Sack
Summary: The activation of KCa2.1-3 calcium-activated potassium channels (SK) requires the interaction of both the N-terminal domain and C-terminal domain of calmodulin. The binding affinity between the N-terminal domain and SK peptide is stronger in the presence of calcium, while the binding affinity of the C-terminal domain is calcium-independent. The calcium-dependent associations of both domains of calmodulin to the SK peptide are crucial events during the gating process.
Article
Biochemistry & Molecular Biology
Shunsuke Ando, Hiroya Mizutani, Makoto Muramatsu, Yumiko Hagihara, Hiroki Mishima, Rubii Kondo, Yoshiaki Suzuki, Yuji Imaizumi, Hisao Yamamura
Summary: This study investigates the role of SKCa and IKCa channels in regulating spontaneous Ca2+ oscillations in rat pinealocytes. The results show that SKCa channels, but not IKCa channels, contribute to the amplitude and frequency of spontaneous Ca2+ oscillations. The activity of SKCa2 channels plays a crucial role in controlling cytosolic Ca2+ signaling and melatonin production in the pineal gland.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Oncology
Chan-Chuan Liu, Cheng-Lin Wu, I-Chun Yeh, Sheng-Nan Wu, Chun- Sze, Po-Wu Gean
Summary: Continuous irradiation-induced resistance (CIIR) in GBM leads to TGF-beta secretion, activation of pro-survival Akt, downregulation of p21 independent of p53, upregulation of multi-drug resistant proteins, enhanced cell mobility and proliferation. CIIR also diminishes BK channel activity in an expression-independent manner. Cilostazol, a phosphodiesterase-3 inhibitor, can reverse CIIR-induced BK channel inactivation, potentially serving as a repurposed treatment for GBM.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Biochemical Research Methods
Emidio Beraldo-Neto, Hugo Vigerelli, Guilherme Rabelo Coelho, Daiane Laise da Silva, Ana Leonor Abrahao Nencioni, Daniel Carvalho Pimenta
Summary: Tityus bahiensis, a scorpion species found in Brazil, has been identified as one of the main causes of human accidents. However, the composition of its venom has not been described in the literature, leading to a lack of proper study on this particular species. In this study, chromatographic separation and proteomic analyses were conducted to investigate the peptides and proteins present in the scorpion venom. The results revealed a diverse range of toxins and enzymes, including potassium and sodium channel toxins, metallopeptidases, hyaluronidases, phospholipase, angiotensin-converting enzyme, cysteine-rich proteins, serine peptidase inhibitors peptides, and antimicrobial peptides. This study provides valuable insights into the diversity and distribution of venom toxins and introduces a straightforward method for venom fractionation.
JOURNAL OF PROTEOMICS
(2023)
Article
Pharmacology & Pharmacy
Saoussen Mlayah-Bellalouna, Dorra Aissaoui-Zid, Aurelie Chantome, Jed Jebali, Soumaya Souid, Emna Ayedi, Hafedh Mejdoub, Maya Belghazi, Naziha Marrakchi, Khadija Essafi-Benkhadir, Christophe Vandier, Najet Srairi-Abid
Summary: The concept of using small conductance Ca2+-activated potassium channels (SKCa) as a pharmacological target for cancer treatment has gained increasing attention in recent years. In this study, researchers isolated the P01 toxin from Androctonus australis (Aa) scorpion venom and investigated its effects on glioblastoma U87, breast MDA-MB231, and colon adenocarcinoma LS174 cancer cell lines. The results showed that P01 specifically inhibited the proliferation, adhesion, and migration of U87 glioblastoma cells, with IC50 values in the micromolar range. Furthermore, P01 was found to reduce the currents recorded in HEK293 cells expressing SK2 channels, but had no effect on cells expressing SK3 channels. This study highlights the potential of scorpion peptides in understanding the role of SKCa channels in tumorigenesis and developing targeted therapeutic molecules for glioblastoma.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Paolo Zuccolini, Loretta Ferrera, Alessia Remigante, Cristiana Picco, Raffaella Barbieri, Sara Bertelli, Oscar Moran, Paola Gavazzo, Michael Pusch
Summary: This research investigated the effect of DCPIB on pancreatic duct adenocarcinoma (PDAC) and melanoma and found that DCPIB directly targeted BK channels and increased intracellular Ca2+ levels. These findings are important for the development of DCPIB-based modulators of ion channels and membrane proteins in the future.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Jade L. Taylor, Harry A. T. Pritchard, Katy R. Walsh, Patrick Strangward, Claire White, David Hill-Eubanks, Mariam Alakrawi, Grant W. Hennig, Stuart M. Allan, Mark T. Nelson, Adam S. Greenstein
Summary: The study investigates the role of vasodilatory pathways in cerebral arteries in the context of Alzheimer's disease. The researchers found that dysfunction in potassium channels involved in vasodilation may contribute to reduced blood flow and impaired functional hyperemia, highlighting the importance of targeting these pathways for potential therapeutic interventions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Hildebrando C. Ferreira-Neto, Javier E. Stern
Summary: Glutamatergic NMDA receptors (NMDARs) and small conductance Ca2+-activated K+ (SK) channels are critical in regulating the activity of hypothalamic magnocellular neurosecretory neurons (MNNs). The functional coupling between NMDARs and SK channels forms a negative feedback loop, which is blunted or absent in MNNs of heart failure (HF) rats, contributing to exacerbated neuronal activity.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Biochemistry & Molecular Biology
Hong T. L. Phan, Hyun J. Kim, Sungwoo Jo, Woo K. Kim, Wan Namkung, Joo H. Nam
Summary: Calcium signaling in T-lymphocytes is regulated by ORAI1 and potassium channels, and Licochalcone A effectively inhibits these channels to suppress T-cell activation and proliferation, making it a potential treatment for inflammation-related immune diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Anatoly Soloviev, Irina Ivanova, Vadym Sydorenko, Khrystyna Sukhanova, Mariia Melnyk, Dariia Dryn, Alexander Zholos
Summary: This study investigates the interaction between gold nanoparticles and cellular structures. The results demonstrate that gold nanoparticles can modulate the activity of potassium channels through changes in intracellular calcium concentration in smooth muscle cells and neurons.
Article
Biology
Pilhwa Lee
Summary: This study investigates the calcium signaling feedback through calcium-activated potassium channels in a dendritic spine using simulation methods. The results show a regulatory mechanism of ion flow permeability by chemical potential barriers, and predict an inhibitory effect on voltage-sensitive calcium channels by calcium-activated potassium channels. The model suggests a potential mechanism of differential calcium handling by channel distributions, providing insights for future computer simulation studies of dendritic spine motility and structural plasticity.
BULLETIN OF MATHEMATICAL BIOLOGY
(2021)
Article
Immunology
Kanika Verma, Rahul Shukla, Jaya Dwivedi, Sarvesh Paliwal, Swapnil Sharma
Summary: Simvastatin has vasorelaxant activity, which involves NO/cGMP pathways, AT(2)R receptors, Ca2+ channels, and K+ channels.
INFLAMMOPHARMACOLOGY
(2023)
Article
Clinical Neurology
Anqi Wang, Yujia Zhou, Huangying Chen, Jiawei Jin, Yingqi Mao, Shuiliang Tao, Tao Qiu
Summary: This study investigated the impact of small-conductance calcium-activated potassium channels (SK channels) on the dopaminergic neuron pathways in the ventral tegmental area (VTA) during post-stroke depression (PSD) and found that inhibiting the SK channels can improve depression symptoms. The results of this study provide new insights into the pathogenesis of PSD and the possibility of developing new strategies to prevent PSD by targeting SK channels.
NEUROPSYCHIATRIC DISEASE AND TREATMENT
(2023)
Article
Multidisciplinary Sciences
Andy K. M. Lam, Jan Rheinberger, Cristina Paulino, Raimund Dutzler
Summary: The study elucidated the conformational changes triggered by the binding of cytoplasmic Ca2+ to the TMEM16A channel and identified three hydrophobic residues as constituents of the channel gate. Mutations in these residues increase Ca2+ potency and result in basal activity, while an activating mutant shows a conformational change that contributes to Ca2+ binding and basal activity. Despite no physical contact, these residues functionally interact to stabilize the gate in the closed conformation, explaining the low open probability of the channel in the absence of Ca2+.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Fumeng Zheng, Haruyuki Kamiya
Summary: Modification of axonal excitability affects information transfer in the brain, but the functional significance of this modulation remains unclear. This study focused on the activity-dependent broadening of action potentials in the hippocampal mossy fibers and found that it is dependent on the inactivation of axonal K+ channels. Computer simulations showed that removing the inactivation process abolished the use-dependent broadening of action potentials. This highlights the critical role of K+ channel inactivation in regulating axonal excitability and short-term plasticity at this synapse.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Behavioral Sciences
Sabrine Aidi-Knani, Imed Regaya, Marianne Amalric, Christiane Mourre
BEHAVIOURAL PHARMACOLOGY
(2015)
Article
Pharmacology & Pharmacy
Sabrine Aidi-Knani, Laurent Pezard, Bedel Mpari, Jeannette Ben Hamida, Jean-Marc Sabatier, Christiane Mourre, Imed Regaya
EUROPEAN JOURNAL OF PHARMACOLOGY
(2015)
Article
Chemistry, Multidisciplinary
Insaf Ayadi, Sandra Mariza Monteiro, Imed Regaya, Ana Coimbra, Fontainhas Fernandes, Maria Manuel Oliveira, Francisco Peixoto, Wissem Mnif
Article
Agricultural Engineering
Kaouther Zaafouri, Manel Ziadi, Rim Ben Farah, Mokhtar Farid, Moktar Hamdi, Imed Regaya
BIOMASS & BIOENERGY
(2016)
Article
Biochemistry & Molecular Biology
Imed Regaya, Nicolas Andreotti, Eric Di Luccio, Michel De Waard, Jean-Marc Sabatier
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2008)
Article
Plant Sciences
Arbia Chalbi, Besma Sghaier-Hammami, Narjes Baazaoui, Sofiene B. M. Hammami, Hatem Ben-Jouira, Pedro Garcia-Caparros, Naceur Djebali, Imed Regaya, Ahmed Debez, Jesus Jorrin-Novo, Chedly Abdelly
Summary: The study on the responses of Cakile maritima to salt stress and fungal infestation showed that plants grown under combined stresses exhibited better growth rate and physiological performance compared to those grown under individual stresses. Salt may play a key role in improving the plant's ability to tolerate fungal attacks.
NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
(2021)
Article
Infectious Diseases
Raoua Ben Brahim, Hasna Ellouzi, Khaoula Fouzai, Nedra Asses, Mohammed Neffati, Jean Marc Sabatier, Philippe Bulet, Imed Regaya
Summary: Extraction methods depend on the chemical nature of the extracted molecule, and selecting the appropriate extraction medium is crucial. This study evaluated several solvents for extracting antimicrobial peptides from extremophile plants, finding acetic acid and sodium acetate to be the most suitable solvents for extraction.
Article
Environmental Sciences
Sofiene Tlili, Dorsaf Jemai, Samar Brinis, Imed Regaya
Article
Biochemistry & Molecular Biology
Imed Regaya, Sabrine Aidi-Knani, Youlet By, Jocelyne Condo, Victoria Gerolami, Jean-Louis Berge-Lefranc, Jeannette Ben Hamida, Jean-Marc Sabatier, Emmanuel Fenouillet, Regis Guieu, Jean Ruf
BIORESEARCH OPEN ACCESS
(2013)
Article
Clinical Neurology
Evelyne Beraud, Angele Viola, Imed Regaya, Sylviane Confort-Gouny, Philippe Siaud, Danielle Ibarrola, Yann Le Fur, Jocelyne Barbaria, Jean-Francois Pellissier, Jean-Marc Sabatier, Igor Medina, Patrick J. Cozzone
ANNALS OF NEUROLOGY
(2006)
Article
Biochemistry & Molecular Biology
I Regaya, C Beeton, G Ferrat, N Andreotti, H Darbon, M De Waard, JM Sabatier
JOURNAL OF BIOLOGICAL CHEMISTRY
(2004)
Article
Medicine, Research & Experimental
I Regaya, T Pham, N Andreotti, N Sauze, L Carrega, MF Martin-Eauclaire, B Jouirou, JC Peragut, H Vacher, H Rochat, C Devaux, JM Sabatier, R Guieu
Article
Biochemistry & Molecular Biology
E Estève, S Smida-Rezgui, S Sarkozi, C Szegedi, I Regaya, LL Chen, X Altafaj, H Rochat, P Allen, IN Pessah, I Marty, JM Sabatier, I Jona, M De Waard, M Ronjat
JOURNAL OF BIOLOGICAL CHEMISTRY
(2003)
Article
Biochemistry & Molecular Biology
S Sarray, N Srairi, M Hatmi, J Luis, H Louzir, I Regaya, H Slema, J Marvaldi, M El Ayeb, N Maffakchi
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2003)