Article
Biochemistry & Molecular Biology
Anett Hudak, Matthew Roach, David Pusztai, Aladar Pettko-Szandtner, Annamaria Letoha, Laszlo Szilak, Mimoun Azzouz, Tamas Letoha
Summary: AAV vectors are safe and efficient gene delivery tools, with AAV9 being capable of crossing the blood-brain barrier for gene transduction in the central nervous system. However, recent studies have revealed limitations in AAV9-mediated gene delivery. This study identified syndecan-4 as a significant contributor to AAV9's cellular internalization, highlighting its role in enhancing gene transduction. By understanding the molecular mechanisms involved, this research provides insights to improve AAV9-based gene therapy approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Juliana Pocas, Catarina Marques, Catarina Gomes, Andreia Hanada Otake, Filipe Pinto, Mariana Ferreira, Tiago Silva, Isabel Faria-Ramos, Rita Matos, Ana Raquel Ribeiro, Emanuel Senra, Bruno Cavadas, Silvia Batista, Joana Maia, Joana A. Macedo, Luis Lima, Luis Pedro Afonso, Jose Alexandre Ferreira, Lucio Lara Santos, Antonio Polonia, Hugo Osorio, Mattias Belting, Celso A. Reis, Bruno Costa-Silva, Ana Magalhaes
Summary: Gastric cancer is influenced by the expression of syndecan-4 (SDC4) which is associated with poor patient survival. SDC4 plays a crucial role in regulating the motility and invasion of gastric cancer cells. Additionally, SDC4 decorated with heparan sulfate is involved in the distribution, uptake, and functional effects of extracellular vesicles (EVs) derived from gastric cancer cells. SDC4 knockout disrupts the tropism of EVs for common gastric cancer metastatic sites. These findings provide insight into the molecular implications of SDC4 expression in gastric cancer cells and present potential therapeutic strategies targeting the glycan-EV axis to limit tumor progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
Haiyi Chen, Yu Kang, Mojie Duan, Tingjun Hou
Summary: The study revealed that the presence of heparan sulfate is crucial for the binding between the spike protein of SARS-CoV-2 and host ACE2, while the stability of the T470-F490 loop and hydrophobic interactions play key roles in the binding process.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Annamaria Letoha, Anett Hudak, Tamas Letoha
Summary: SARS-CoV-2 variants, particularly Delta and Omicron, exhibit differential cellular uptake mechanisms, with Omicron relying primarily on HSPGs for entry. Delta variant benefits from SDC1, 2, and 4 overexpression, while SDC4 has the most prominent effect on Omicron internalization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
John R. Couchman
Summary: Cell surface proteoglycans, such as syndecans, play crucial roles in regulating cell behavior, including interactions with extracellular matrix components and mediating proliferation, adhesion, and migration. In tumors, changes in the levels and distribution of syndecan-1 can have implications for prognosis, with loss of membrane staining and abnormal cytoplasmic or nuclear staining often indicating poor outcomes. Targeting syndecan-1 with antibody-toxin conjugates may hold promise for clinical applications in both myeloma and some carcinomas.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anett Hudak, Gabor Veres, Annamaria Letoha, Laszlo Szilak, Tamas Letoha
Summary: Emerging SARS-CoV-2 Delta variant poses a threat to COVID-19 vaccination campaigns. Research shows that the mutations in the Delta spike protein increase its affinity for heparan sulfate proteoglycans and its interaction with syndecan-4, which enhances its cellular entry and facilitates its transmission.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Ghadah A. Karasneh, Divya Kapoor, Navya Bellamkonda, Chandrashekhar D. Patil, Deepak Shukla
Summary: The increased levels of HPSE can reduce syncytial plaque formation and promote viral egress and release. Transiently enhanced expression of HPSE does not affect HSV-1 entry into cells but facilitates extracellular release of mature virions.
Article
Geriatrics & Gerontology
Maria De Luca, David Ronald Bryan, Gary Richard Hunter
Summary: Aging is accompanied by low-grade proinflammatory status. This study found significant associations of SDC4 with MMP-9, cardiovascular hemodynamic phenotypes, heart rate, and blood pressure, but not with proinflammatory cytokines or arterial elasticity.
AGING CLINICAL AND EXPERIMENTAL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Antonia Charchanti, Panagiotis Kanavaros, Efthymios Koniaris, Agapi Kataki, Georgios Glantzounis, Niki J. Agnantis, Anna C. Goussia
Summary: In patients with chronic liver diseases, the immunohistochemical overexpression of syndecan-1 was found to be positively correlated with the severity of fibrosis, indicating its potential role in hepatic fibrogenesis.
Article
Biophysics
Bruna G. Carvalho, Bianca B. M. Garcia, Antonio A. Malfatti-Gasperini, Sang W. Han, Lucimara G. de la Torre
Summary: The association between chondroitin sulfate (CS) and cationic liposomes (CLs)/lipoplex (CL-pDNA) was analyzed in this study. The results showed that the electrostatic interaction between CS and CL/CL-pDNA could reorganize the lipid bilayers and form nanoscale vesicles. These findings have implications for the development of novel carriers for drug and gene delivery applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Cell Biology
Jessica Oyie Sousa Onyeisi, Carla Cristina Lopes, Martin Goette
Summary: Aberrant expression of syndecan-4 is associated with worse prognosis in breast cancer. It is regulated by various mechanisms, including transcriptional and posttranscriptional mechanisms. Syndecan-4 plays a crucial role in breast cancer progression by interacting with different ligands and regulating cellular processes such as adhesion, migration, and invasion. Additionally, syndecan-4 expression is modulated by drugs used in breast cancer treatment.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yoshio Hattori, Masahiro Hasegawa, Takahiro Iino, Kyoko Imanaka-Yoshida, Akihiro Sudo
Summary: In this study, the researchers found that treating osteoarthritic cartilage with SDC4 can inhibit cartilage degeneration. They discovered that SDC4 upregulated the expression of TIMP-3 and downregulated the expression of MMP-13 and ADAMTS-5 in chondrocytes. Additionally, intra-articular injection of SDC4 in an OA mouse model prevented articular cartilage degeneration.
Article
Multidisciplinary Sciences
Natalie Acker, Harold Smith, Claire Devine, Sharon L. Oltjen, Sofia Tsiropoulou, Zeljka Smit-McBride, Karen Lange, Oliver E. Blacque, Joanne A. Matsubara, Andrew Gordus, Andy Golden, Bruce E. Vogel
Summary: Age-related macular degeneration is a leading cause of blindness among the elderly, with defective CFH and HS interactions potentially contributing to the progression of AMD. Studies indicate that CFH plays a crucial role in maintaining ciliary structure, which can help prevent the development of AMD.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Hematology
Linda M. Hiebert
Summary: Diabetes is a complex disease that affects millions of people worldwide, and the role of proteoglycans, particularly heparan sulfate proteoglycans (HSPGs), in its pathogenesis has recently gained attention. HSPGs play crucial roles in various physiological processes, and their modification in diabetes can lead to complications in different tissues and organs. Understanding the role of HSPGs in diabetes may help develop new treatments and preventive measures for this condition.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2021)
Article
Medical Laboratory Technology
V. I. G. N. E. S. H. MARIAPPAN, S. H. A. L. I. N. D. A. ADIKARI, L. O. K. E. S. H. SHANMUGAM, J. O. S. H. Y. M. EASOW, A. G. I. E. S. H. K. U. M. A. R. B. A. L. A. K. R. I. S. H. N. A. PILLAI
Summary: Protein and mRNA levels of ENG and SDC-1 increase significantly towards defervescence in dengue cases, with higher levels in severe dengue cases compared to controls. Support Vector Machine analysis shows that serum levels of ENG and SDC-1, along with other clinical symptoms, can predict disease severity with 100% accuracy.
TRANSLATIONAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Bettina Ughy, Ildiko Schmidthoffer, Laszlo Szilak
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Daniel Becsky, Szuzina Gyulai-Nagy, Arpad Balind, Peter Horvath, Laszlo Dux, Aniko Keller-Pinter
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Meeting Abstract
Clinical Neurology
Daniel Becsky, Kitti Szabo, Szuzina Gyulai-Nagy, Tamas Gajdos, Zsuzsa Bartos, Arpad Balind, Laszlo Dux, Peter Horvath, Miklos Erdelyi, Laszlo Homolya, Aniko Keller-Pinter
NEUROMUSCULAR DISORDERS
(2020)
Article
Cell Biology
Daniel Becsky, Kitti Szabo, Szuzina Gyulai-Nagy, Tamas Gajdos, Zsuzsa Bartos, Arpad Balind, Laszlo Dux, Peter Horvath, Miklos Erdelyi, Laszlo Homolya, Aniko Keller-Pinter
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell Biology
Csaba Matta, Rebecca Lewis, Christopher Fellows, Gyula Diszhazi, Janos Almassy, Nicolai Miosge, James Dixon, Marcos C. Uribe, Sean May, Szilard Poliska, Richard Barrett-Jolley, Janos Fodor, Peter Szentesi, Tibor Hajdu, Aniko Keller-Pinter, Erin Henslee, Fatima H. Labeed, Michael P. Hughes, Ali Mobasheri
Summary: Chondrogenic progenitor cells (CPCs) may have superior chondrogenic potential compared to mesenchymal stem cells (MSCs), with a distinct channelome characteristic, including calcium-dependent potassium channels such as BK channels. The KCNMA1 gene was found to be upregulated in CPCs and associated with BK channel activity, impacting proliferation, gene expression, migration, and calcium events in CPCs. These findings contribute to a better understanding of CPCs and their potential in cartilage regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Oncology
Aniko Keller-Pinter, Szuzina Gyulai-Nagy, Daniel Becsky, Laszlo Dux, Laszlo Rovo
Summary: Cell migration is essential for the formation of metastasis and is a key feature of malignancy. Syndecan-4 (SDC4) plays a crucial role in cell migration by regulating various signaling pathways and biological processes. Its involvement in tumor progression has been demonstrated, highlighting its significance in cell movement and metastasis.
Article
Oncology
Tibor Hajdu, Patrik Kovacs, Emese Zsigrai, Roland Takacs, Judit Vago, Sinyoung Cho, Laszlo Sasi-Szabo, Daniel Becsky, Aniko Keller-Pinter, Gabriella Emri, Kalman Racz, Dora Reglodi, Roza Zakany, Tamas Juhasz
Summary: The study confirms the expression of PACAP receptors in melanocytes and melanoma cells, demonstrating that PACAP can inhibit proliferation and migration of melanoma cells, making it a potential target in melanoma progression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kitti Szabo, Daniel Varga, Attila Gergely Vegh, Ning Liu, Xue Xiao, Lin Xu, Laszlo Dux, Miklos Erdelyi, Laszlo Rovo, Aniko Keller-Pinter
Summary: This study found that reduced expression of syndecan-4 enhances myoblast differentiation and fusion in skeletal muscle development and regeneration. It also suggests that syndecan-4 may serve as a tumor driver gene in promoting rhabdomyosarcoma tumor development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Monika Goenczi, Andrea Csemer, Laszlo Szabo, Monika Sztretye, Janos Fodor, Krisztina Pocsai, Kalman Szenthe, Aniko Keller-Pinter, Zoltan Marton Koehler, Peter Nanasi, Norbert Szentandrassy, Balazs Pal, Laszlo Csernoch
Summary: Astaxanthin, a lipid-soluble carotenoid, has significant effects on lipid metabolism, body weight, and insulin sensitivity. It increases food intake, affects protein levels and metabolism, and strengthens inhibitory inputs in neuronal populations. Both acute and chronic treatment with Astaxanthin can reduce cardiac action potentials and fatty acid biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eszter Becskehazi, Marietta Margareta Korsos, Eleonora Gal, Laszlo Tiszlavicz, Zsofia Hoyk, Maria A. Deli, Zoltan Marton Kohler, Aniko Keller-Pinter, Attila Horvath, Kata Cseko, Zsuzsanna Helyes, Peter Hegyi, Viktoria Venglovecz
Summary: The study suggests that smoking affects the function of ion transporters, playing a role in the development of smoking-induced esophageal diseases. In metaplastic cells, CSE treatment increased the activity and protein expression of NHE-1, reducing proliferation in a NHE-1-dependent manner. Contrarily, in normal cells, the expression and activity of NHE-1 decreased due to smoke exposure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Dezso P. Virok, Ferenc Tomosi, Aniko Keller-Pinter, Kitti Szabo, Anita Bogdanov, Szilard Poliska, Zsolt Razga, Bella Bruszel, Zsuzsanna Cseh, David Kokai, Dora Paroczai, Valeria Endresz, Tamas Janaky, Katalin Burian
Summary: Neutrophil gene expression was significantly altered by C. trachomatis infection, inducing 1295 genes and repressing 1510 genes. Although the tryptophan-degrading activity of IDO1 was greatly increased by the addition of IFNG, it was not able to inhibit chlamydial growth in human neutrophils. Further investigation is needed to determine whether other chlamydial growth-promoting mechanisms were induced in the infected and interferon-treated neutrophils.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mohamed M. Abdelghafour, Agota Deak, Tamas Kiss, Gabor Katona, Rita Ambrus, Aniko Keller-Pinter, Istvan Szatmari, Diana Szabo, Laszlo Rovo, Laszlo Janovak, Maria Budai-Szfics, Mint Lorinczi
Summary: A two-component injectable hydrogel was developed for the encapsulation and prolonged release of an antimuscular atrophy drug. The hydrogel exhibited controlled release characteristics and increased adhesion properties, making it a potential candidate for drug delivery systems.
Article
Biochemistry & Molecular Biology
Monika Sztretye, Zoltan Singlar, Nyamkhuu Ganbat, Dana Al-Gaadi, Kitti Szabo, Zoltan Marton Kohler, Laszlo Dux, Aniko Keller-Pinter, Laszlo Csernoch, Peter Szentesi
Summary: The remodelling of the extracellular matrix plays a crucial role in skeletal muscle development and regeneration. The decreased expression of Syndecan-4 leads to reduced muscle performance and altered calcium homeostasis in mice. These effects are observed at both young and aged stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Bettina Ughy, Sarolta Nagyapati, Dezi B. Lajko, Tamas Letoha, Adam Prohaszka, Dima Deeb, Andras Der, Aladar Pettko-Szandtner, Laszlo Szilak
Summary: The growth of bacterial populations does not solely consist of continuous reproduction and cell death. Instead, there is a stationary phase during the growth of bacterial populations, which does not result from accumulated toxins or cell death. During the stationary phase, the phenotype of the cells changes while the total cell concentration remains constant, but the colony forming unit (CFU) decreases over time. Bacterial populations can be seen as virtual tissues formed through a specific differentiation process, where exponential-phase cells develop into stationary-phase cells and eventually become unculturable. The richness of nutrients does not affect the growth rate or stationary cell density. Generation time is not a constant value, but depends on the concentration of starter cultures. Inoculations with serial dilutions of stationary populations reveal a minimal stationary cell concentration (MSCC) point, below which cell concentrations remain constant upon dilution, which seems to be a universal phenomenon among unicellular organisms.
Article
Biochemistry & Molecular Biology
Balazs Sonkodi, Laszlo Marsovszky, Anita Csorba, Attila Balog, Bence Kopper, Aniko Keller-Pinter, Zoltan Zsolt Nagy, Miklos D. Resch
Summary: This study aimed to analyze corneal neural regeneration in ankylosing spondylitis patients and its correlation with Langerhans cell density, morphology, and dry eye parameters. The results showed that corneal nerve-fiber characteristics were lower in ankylosing spondylitis patients, indicating disrupted neural regeneration. Peripheral Langerhans cell density was negatively correlated with nerve fiber characteristics. Tear film break-up time was negatively correlated with corneal nerve fiber total branch density.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Xuezhi Zhuo, Vito Fodera, Per Larsson, Zarah Schaal, Christel A. S. Bergstrom, Korbinian Lobmann, Aleksei Kabedev
Summary: Our previous work demonstrated that beta-lactoglobulin-stabilized amorphous solid dispersion (ASD) loaded with 70 % indomethacin remains stable for over 12 months. We further investigated the stabilization mechanisms by testing five other drug molecules and using experimental techniques and molecular dynamics simulations. The results showed that steric confinement, hydrogen bonding, and the glass transition temperature of the drug molecule play important roles in stabilizing ASDs with high drug loadings.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Article
Pharmacology & Pharmacy
Sebastian Schmidt, Ulrike Holzgrabe
Summary: The binding of drugs to plasma proteins, such as human serum albumin (HSA), is crucial for determining pharmacokinetic parameters. This study investigated the enantioselective binding of S- and R-ketamine to HSA. It was found that ketamine has weak affinity to HSA, with no significant differences in binding behavior between the individual enantiomers and the racemate. The aromatic ring and N-methyl group were identified as the most strongly involved structural moieties in the binding of ketamine to HSA.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Article
Pharmacology & Pharmacy
Yuchen Zhao, Han Wang, Lin Jin, Ziwei Zhang, Lianghu Liu, Mengqi Zhou, Xianzheng Zhang, Lingling Zhang
Summary: Interleukins (ILs) are important for communication between immune cells and non-immune cells, but dysregulation of ILs expression is a characteristic of autoinflammatory diseases. Drugs targeting ILs have significant clinical benefits, but may also cause adverse reactions. Fusion protein technology, with its ability to enhance therapeutic efficacy, has been explored for developing anti-inflammatory drugs. This review discusses the efficacy of fusion protein drugs developed with ILs or their receptors in treating autoinflammatory diseases, highlighting the potential of this technology in future anti-inflammatory drug development.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Article
Pharmacology & Pharmacy
Serena Bertoni, Elena Simone, Stefano Sangiorgi, Beatrice Albertini, Nadia Passerini
Summary: This study investigated the correlation between the structure and release properties of solid lipid microparticles (MPs) with different liquid additives. The additives accelerated the conversion of the unstable alpha-form of tristearin to the stable beta-polymorph and caused structural modifications in the MPs. The presence of additives prolonged the drug release in water and resulted in higher release profiles in biorelevant media. The findings suggest that the release behavior can be influenced by the polymorphism and supramolecular-level structural modification of lipid formulations containing crystal modifiers.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Article
Pharmacology & Pharmacy
Juulia Jarvinen, Ahmed B. Montaser, Santosh Kumar Adla, Jukka Leppanen, Marko Lehtonen, Kati-Sisko Vellonen, Tuomo Laitinen, Aaro Jalkanen, William F. Elmquist, Juri Timonen, Kristiina M. Huttunen, Jarkko Rautio
Summary: This study attempted to alter the brain distribution pattern of Palbociclib by creating and assessing two novel prodrugs. Although the prodrug design did not significantly improve Palbociclib brain delivery, the study provides valuable insights for future prodrug development and drug delivery strategies targeting specific transporters.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Review
Pharmacology & Pharmacy
Miao Wang, Xinyu Ma, Shiyu Zong, Yaqiong Su, Rui Su, Hong Zhang, Yang Liu, Chunliu Wang, Ye Li
Summary: This article discusses the potential and limitations of nasal administration in central nervous system drug delivery. Nasal gel viscosity can alleviate the impact of nasal mucociliary clearance on drug delivery, and materials such as gellan gum, chitosan, carbomer, cellulose, and poloxamer can be used to prepare nasal gels.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Article
Pharmacology & Pharmacy
Bjarke Strom Larsen, Eric Kissi, Liebert Parreiras Nogueira, Natalja Genina, Ingunn Tho
Summary: This study investigates the influence of drug load and polymer molecular weight on the structure of 3D printed tablets. The results show that drug load and polymer molecular weight have a significant impact on the porosity and size of the tablets, while the effect of drug load on the total porosity of the tablets is minimal.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Article
Pharmacology & Pharmacy
Zhentao Qiao, Fuhang Wang, Dongjian Han, Yuansong Zhuang, Qingjiao Jiang, Yi Zhang, Miaomiao Liu, Quanxu An, Zhiwei Wang, Deliang Shen
Summary: In this study, it was demonstrated that periadventitial delivery of rapamycin-fibrin glue (RPM-FG) can inhibit intimal hyperplasia (IH) in a rat carotid artery injury model without compromising re-endothelialization. This provides a promising direction for the future development of a safe, effective, and minimally invasive perivascular drug delivery method to treat vascular disease.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)
Article
Pharmacology & Pharmacy
Neele Puhlmann, Rodrigo Vidaurre, Klaus Kuemmerer
Summary: Active pharmaceutical ingredients and their metabolites and transformation products are pollutants that can harm human and environmental health. Designing greener APIs is an effective strategy to address this issue. The drug discovery and development process can incorporate environmental parameters to achieve this design, and this process is highly flexible.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2024)