Journal
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 48, Issue 1-2, Pages 202-210Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejps.2012.10.018
Keywords
Drug targeting; mTHPC; High content analysis; Encapsulation; Cytotoxicity
Categories
Funding
- Health Research Board [2007 TRA/2007/11]
- Science Foundation Ireland [SFI P.I. 09/IN.1/B2650, 07/SRC/ B1154]
- Fundacao da Ciencia e Tecnologia [SFRH/BD/62364/2009]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/62364/2009] Funding Source: FCT
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Photodynamic therapy (PDT) is based on the delivery of photocytotoxic agents to a target tissue, followed by irradiation. In order to increase the efficiency of PDT in oesophageal cancer therapy, polyethylene glycol (PEG)-grafted, transferrin (TO-conjugated liposome formulations of 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (Foscan), a second-generation photosensitiser, were prepared. Expression of transferrin receptors (CD71) in the oesophageal cancer cell line, OE21, was confirmed by immunoblot and confocal laser scanning microscopy. The anti-proliferative effect of Foscan liposomes was evaluated and compared with plain formulations (i.e., without If) as well as with free drug. In addition, the intracellular accumulation was studied using high content analysis. Surprisingly, delivering Foscan by transferrin-conjugated PEG-liposomes to oesophageal cancer cells did not improve the photocytotoxicity or the intracellular accumulation of Foscan when compared to unmodified liposomes or indeed free photosensitiser. Tf-targeted drugs and drug delivery systems have shown improved the therapy of many cancers. Our study, however, did not corroborate these findings. If this is due to the tumour type, the choice of in vitro model or the delivery systems remains to be confirmed. (C) 2012 Elsevier B.V. All rights reserved.
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