Article
Clinical Neurology
Qian Chen, Kai Ma, Bin Wang, Yongquan Chen
Summary: This study observed the effects of direct suppression of the parabrachial nucleus (PBN) on chronic neuropathic pain (CNP) and CNP-related behaviors in mice. The results showed that CNP induced anxiety-like behavior and increased PBN-induced pain in mice, but direct inhibition of PBN neuron activity alone did not improve CNP or CNP-related behavior.
JOURNAL OF CLINICAL NEUROSCIENCE
(2022)
Review
Neurosciences
Andrew E. Warfield, Jonathan F. Prather, William D. Todd
Summary: Research suggests a bidirectional relationship between circadian rhythms and chronic pain, with a focus on limited molecules or cell types rather than system-level interactions. Gender differences are key in circadian rhythms and chronic pain research.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jinhong Jiang, Lingfei Xu, Long Yang, Su Liu, Zhe Wang
Summary: MOTS-c, a mitochondrial-derived peptide, plays an important role in physiological and pathological functions through AMPK activation. This study found that MOTS-c levels were lower in mice with neuropathic pain compared to control mice. MOTS-c treatment dose-dependently reduced pain sensitivity in these mice via the activation of AMPK and inhibition of proinflammatory cytokine production and microglia activation.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Clinical Neurology
Eduardo Benarroch
Summary: The dopaminergic neurons in the ventral tegmental area (VTA) play a role in the perception and modulation of chronic pain. Recent studies in rodents using various techniques have provided insights into the dysfunction of the midbrain dopaminergic system in chronic pain. This has clinical relevance and has been indirectly demonstrated in humans using neuroimaging techniques.
Article
Neurosciences
Jing Li, Chao Tian, Shiyang Yuan, Zhenyu Yin, Liangpeng Wei, Feng Chen, Xi Dong, Aili Liu, Zhenhuan Wang, Tongrui Wu, Chunxiao Tian, Lin Niu, Lei Wang, Pu Wang, Wanyu Xie, Fujiang Cao, Hui Shen
Summary: The reorganization of the primary sensory cortex (S1) and the regulatory mechanism of the lateral parabrachial nucleus (lPBN) in neuropathic pain after spinal cord injury (SCI) were investigated. It was found that changes in neuronal activity in S1 are closely related to limb pain, and these symptoms can be regulated by lPBN.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Neurosciences
Valeria Mussetto, Aurora Moen, Lidia Trofimova, Juergen Sandkuehler, Roni Hogri
Summary: The clinical burden faced by chronic pain patients is compounded by affective comorbidities, such as depression and anxiety disorders. Emerging evidence suggests that reactive glial cells in the spinal cord dorsal horn play a key role in the chronification of pain, while supraspinal glia are important for psychological aspects of chronic pain.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Anatomy & Morphology
James W. M. Kang, David Mor, Kevin A. Keay
Summary: The amygdala is crucial for appropriate responses to emotional or stressful stimuli, and both chronic pain and acute stress have been shown to independently modulate its activity. This study investigated the effects of a neuropathic injury on amygdala activation to an acute stressor, revealing specific regulation of the amygdala to prefrontal cortex pathway by the combination of injury and stress. Chronic constriction injury resulted in increased neuronal activation in the basolateral amygdala, potentially reflecting sensitization due to nerve injury and heightened sensitivity to emotional/psychological stress.
BRAIN STRUCTURE & FUNCTION
(2021)
Article
Biochemistry & Molecular Biology
Lin Wu, Yujie Wu, Jin Liu, Jingyao Jiang, Cheng Zhou, Donghang Zhang
Summary: The elevated excitability of glutamatergic neurons in the lateral parabrachial nucleus (PBL) is found to be associated with the development of inflammatory pain. In this study, chemogenetic manipulation was used to explore the relationship between PBL glutamatergic neuron activity and pain thresholds. The results demonstrate that activation of PBL glutamatergic neurons decreases pain thresholds, while inhibition produces the opposite effect. The increased expression of C-Fos protein and NALCN in PBL glutamatergic neurons is also observed in the model of inflammatory pain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Huan-Yu Zheng, Yu-Meng Chen, Yao Xu, Cheng Cen, Yun Wang
Summary: The study found that acute inflammatory pain impairs sustained attention, but chronic inflammatory pain does not. Inhibition of the ascending pain pathway or chemogenetic inhibition of CaMKII alpha neurons in the lateral parabrachial nucleus can attenuate the acute inflammatory pain-induced performance impairment, which affects the recovery of 3CSRTT performance during chronic inflammatory pain.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Pauravi J. Gandhi, Dinesh Y. Gawande, Gajanan P. Shelkar, Sukanya G. Gakare, Takaki Kiritoshi, Guangchen Ji, Bishal Misra, Ratnamala Pavuluri, Jinxu Liu, Volker Neugebauer, Shashank M. Dravid
Summary: Chronic pain is associated with synaptic dysfunction in the amygdala due to impaired GluD1-Cbln1 signaling. Injection of Cbln1 in the central amygdala can alleviate behavioral pain parameters and normalize neuronal excitability in pain models.
Article
Neurosciences
Hang Zhou, Miao Li, Ruohe Zhao, Linlin Sun, Guang Yang
Summary: Poor sleep is associated with the risk of developing chronic pain. In a mouse model of neuropathic pain, cholinergic neurons in the basal forebrain are increasingly active during nonrapid eye movement (NREM) sleep. These neurons directly activate vasoactive intestinal polypeptide-expressing interneurons in the primary somatosensory cortex (S1), causing pain. The hyperactivity of these neurons is caused by inputs from the parabrachial nucleus (PB) driven by the injured peripheral nerves.
NATURE NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Shijia Liu, Dong-Il Kim, Tae Gyu Oh, Gerald M. Pao, Jong-Hyun Kim, Richard D. Palmiter, Matthew R. Banghart, Kuo-Fen Lee, Ronald M. Evans, Sung Han
Summary: Neurons expressing the mu-opioid receptor within the lateral parabrachial nucleus play a crucial role in opioid-induced respiratory depression (OIRD). Chemogenetic modulation of these neurons can rescue respiratory rhythms and serve as a potential therapeutic target for treating OIRD in patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Clinical Neurology
Hannah Luise Teuchmann, Roni Hogri, Bernhard Heinke, Jurgen Sandkuehler
Summary: The lateral parabrachial nucleus (LPBN) is crucial for processing pain aversion, and opioids, cannabinoids, and benzodiazepines have different effects on LPBN synapses, offering new insights for targeted therapies in pain management.
Article
Neurosciences
Eric Brown, Ayma F. Malik, Elizabeth R. Moese, Abigail F. McElroy, Angelo C. Lepore
Summary: Neuropathic pain (NP) is a common and debilitating comorbidity of spinal cord injury (SCI). This study investigates the neural activity changes in the pain circuitry following cervical spinal cord injury. The findings reveal complex changes in neuronal activation, with increased activation in some neurons and decreased activation in inhibitory neurons.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Plant Sciences
Ananya Buapratoom, Aree Wanasuntronwong, Onrawee Khongsombat, Mayuree H. Tantisira
Summary: ECa 233 showed anti-nociceptive effects on chronic neuropathic orofacial pain in mice by modulating peripheral calcitonin gen-related peptide expression, which decreased nociceptive activity in trigeminal nucleus caudalis. This study also found a synergistic effect of ECa 233 with gabapentin, reducing mechanical hyperalgesia and allodynia in mice. Further clinical trials are needed to explore the efficacy of ECa 233 in treating neuropathic pain in humans.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Cell Biology
Adriana-Natalia Murgoci, Marie Duhamel, Antonella Raffo-Romero, Khalil Mallah, Soulaimane Aboulouard, Christophe Lefebvre, Firas Kobeissy, Isabelle Fournier, Monika Zilkova, Denisa Maderova, Milan Cizek, Dasa Cizkova, Michel Salzet
JOURNAL OF EXTRACELLULAR VESICLES
(2020)
Article
Biochemistry & Molecular Biology
Zuzana Vikartovska, Maria Kuricova, Jana Farbakova, Tomas Liptak, Dagmar Mudronova, Filip Humenik, Aladar Madari, Marcela Maloveska, Eva Sykova, Dasa Cizkova
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Critical Care Medicine
Elizabeth A. Dugan, Benjamin Schachner, Stanislava Jergova, Jacqueline Sagen
Summary: Research shows that high-intensity locomotor training can significantly reduce neuropathic pain after spinal cord injury and maintain the reduction effect for at least two years, while improving gait outcomes and prolonging lifespan for rats.
JOURNAL OF NEUROTRAUMA
(2021)
Article
Veterinary Sciences
Zuzana Vikartovska, Jana Farbakova, Tomas Smolek, Jozef Hanes, Norbert Zilka, Lubica Hornakova, Filip Humenik, Marcela Maloveska, Nikola Hudakova, Dasa Cizkova
Summary: A comprehensive approach using CADES questionnaire, biochemical analyses and blood serum biomarkers can enable early diagnosis of canine cognitive dysfunction syndrome (CCDS) in senior dogs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Dasa Cizkova, Milan Cizek, Marcela Maloveska, Marta Kmetova, Vladimir Kmet, Dobroslava Bujnakova
Summary: The findings suggest that cell-free L. casei 21L10 supernatant may have potential in attenuating LPS-induced inflammation, inhibiting proliferation, and promoting apoptosis/necrosis. While this study provides insight into using L. casei exoproducts as anti-inflammatory or anti-proliferative agents for gut diseases, further data is required to confirm their efficacy.
Review
Spectroscopy
Khalil Mallah, Kazem Zibara, Coline Kerbaj, Ali Eid, Nour Khoshman, Zahraa Ousseily, Abir Kobeissy, Tristan Cardon, Dasa Cizkova, Firas Kobeissy, Isabelle Fournier, Michel Salzet
Summary: Traumatic brain injury (TBI) is a major public health concern worldwide, and the use of proteomic and lipidomic platforms has broadened our understanding of TBI-related mechanisms and neuropathological complications. This study provides an updated overview of spatially resolved microproteomics and microlipidomics approaches guided by mass spectrometry imaging in TBI studies and discusses their applications in neurotrauma research. The accurate and precise techniques of microproteomic sampling, such as laser capture microdissection, parafilm assisted microdissection, and liquid microjunction extraction, are evaluated. The potential of lipid profiling, particularly in phospholipid metabolism and proinflammatory molecules, is highlighted.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Behavioral Sciences
Stanislava Jergova, Cecilia Perez, Julita S. Imperial, Shyam Gajavelli, Aakangsha Jain, Adam Abin, Baldomero M. Olivera, Jacqueline Sagen
Summary: This study found that two venom extracts from Conus showed potential in interacting with the CB1 receptor, demonstrating effectiveness in analgesia and impact on nerve injury models. Further research revealed the presence of potential components in these venoms for the treatment of chronic pain, with mild side effects in the CB tetrad assessment.
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
(2021)
Review
Cell Biology
Eva Sykova, Dasa Cizkova, Sarka Kubinova
Summary: MSCs, as candidates in regenerative medicine, have shown significant therapeutic potential in animal experiments and clinical studies due to their immunomodulatory, paracrine, and neural stimulation effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Microbiology
Nikola Hudakova, Simona Hricikova, Amod Kulkarni, Mangesh Bhide, Eva Kontsekova, Dasa Cizkova
Summary: A year after the outbreak of the pandemic, treatments and vaccines for COVID-19 have continued to progress, with licensed vaccines being the most effective weapon against the virus. However, limitations in production and delivery of vaccines in some countries pose challenges in achieving widespread vaccination coverage.
Article
Agriculture, Dairy & Animal Science
Filip Humenik, Marcela Maloveska, Nikola Hudakova, Patricia Petrouskova, Lubica Hornakova, Michal Domaniza, Dagmar Mudronova, Simona Bodnarova, Dasa Cizkova
Summary: This study compares the differences in morphology, phenotype, multilineage potential, and proliferation capacity of canine MSCs isolated from bone marrow, adipose tissue, and amniotic tissue. The results show variations in these characteristics among the MSCs from different sources, and suggest considerations for isolation, expansion, and phenotyping of these cells prior to their application in regenerative veterinary medicine.
Article
Biotechnology & Applied Microbiology
Stanislava Jergova, Elizabeth A. Dugan, Jacqueline Sagen
Summary: This study found that chronic pain caused by spinal cord injury is related to dysfunctional GABAergic signaling and enhanced NMDA signaling. In a rat model, grafting recombinant rat GABAergic cells into the spinal cord and intensive locomotor training can reduce pain sensitivity. These treatments can attenuate tactile, heat, and cold hypersensitivity and reduce levels of proinflammatory cytokines. The analgesic effects of cell therapy and locomotor training persist even after the training is discontinued. Therefore, intermittent training combined with cell therapy may be an effective approach to manage chronic pain in spinal cord injury patients.
BIOENGINEERING-BASEL
(2023)
Correction
Cell Biology
Alice Capuz, Sylvain Osien, Tristan Cardon, Melodie Anne Karnoub, Soulaimane Aboulouard, Antonella Raffo-Romero, Marie Duhamel, Dasa Cizkova, Marco Trerotola, David Devos, Firas Kobeissy, F. Vanden Abeele, Amelie Bonnefond, Isabelle Fournier, Franck Rodet, Michel Salzet
CELL DEATH & DISEASE
(2023)
Article
Agriculture, Dairy & Animal Science
Michal Domaniza, Marian Hluchy, Dasa Cizkova, Filip Humenik, Lucia Slovinska, Nikola Hudakova, Lubica Hornakova, Juraj Vozar, Alexandra Trbolova
Summary: This study aimed to investigate the potential of cell-based regenerative therapy for elbow joints affected by osteoarthritis. The results showed that two injections of amnion-derived mesenchymal stem cells improved joint range of motion and had an impact on synovial fluid biomarkers. Concentrations of IFN-γ, IL-6, and TNF-α decreased while IL-10 increased between day 0 and day 28 after treatment.
Article
Neurosciences
Stanislava Jergova, Melissa Hernandez, Jacqueline Sagen
Summary: This study demonstrates the feasibility of using transplantable recombinant GABAergic cells releasing conopeptide MVIIA to treat chronic pain, with beneficial effects observed in reducing hypersensitivity and pain-related cytokine levels.
Article
Clinical Neurology
Stanislava Jergova, Heidy Martinez, Melissa Hernandez, Benjamin Schachner, Suzanne Gross, Jacqueline Sagen
Summary: This study used Sprague-Dawley male rats to mimic neuropathic and inflammatory pain scenarios in a pre-amputation setting, finding that injuries prior to axotomy resulted in more severe PLP behavior directed towards the pretreatment insult origin. The study also observed changes in biomarkers related to the severity of PLP outcomes.
FRONTIERS IN PAIN RESEARCH
(2021)
