Journal
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Volume 77, Issue -, Pages 38-46Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2014.02.034
Keywords
Fragment screening; Fragment docking; Virtual screening; Ensemble docking; G protein-coupled receptors; Dopamine D3 receptor; Histamine H4 receptor
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Funding
- COST Action [CM1207]
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Prospective structure based virtual fragment screening methodologies on two GPCR targets namely the dopamine D3 and the histamine H4 receptors with a library of 12,905 fragments were evaluated. Fragments were docked to the X-ray structure and the homology model of the D3 and H4 receptors, respectively. Representative receptor conformations for ensemble docking were obtained from molecular dynamics trajectories. In vitro confirmed hit rates ranged from 16% to 32%. Hits had high ligand efficiency (LE) values in the range of 0.31-0.74 and also acceptable lipophilic efficiency. The X-ray structure, the homology model and structural ensembles were all found suitable for docking based virtual screening of fragments against these GPCRs. However, there was little overlap among different hit sets and methodologies were thus complementary to each other. (C) 2014 Elsevier Masson SAS. All rights reserved.
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