Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 44, Issue 2, Pages 500-510Publisher
WILEY
DOI: 10.1002/eji.201343820
Keywords
DCs; GM-CSF; IFN- receptor; IL-4; Spleen
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Funding
- Deutsche Forschungsgemeinschaft [SFB 704]
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The DC-derived chemokine CCL17, a ligand of CCR4, has been shown to promote various inflammatory diseases such as atopic dermatitis, atherosclerosis, and inflammatory bowel disease. Under steady-state conditions, and even after systemic stimulation with LPS, CCL17 is not expressed in resident splenic DCs as opposed to CD8(-)CD11b(+) LN DCs, which produce large amounts of CCL17 in particular after maturation. Upon systemic NKT cell activation through -galactosylceramide stimulation however, CCL17 can be upregulated in both CD8(-) and CD8(+) splenic DC subsets and enhances cross-presentation of exogenous antigens. Based on genome-wide expression profiling, we now show that splenic CD11b(+) DCs are susceptible to IFN--mediated suppression of CCL17, whereas LN CD11b(+)CCL17(+) DCs downregulate the IFN-R and are much less responsive to IFN-. Under inflammatory conditions, particularly in the absence of IFN- signaling in IFN-RKO mice, CCL17 expression is strongly induced in a major proportion of splenic DCs by the action of GM-CSF in concert with IL-4. Our findings demonstrate that the local cytokine milieu and differential cytokine responsiveness of DC subsets regulate lymphoid organ specific immune responses at the level of chemokine expression.
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