Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 43, Issue 9, Pages 2473-2483Publisher
WILEY
DOI: 10.1002/eji.201242676
Keywords
CEACAM1; Cytolysis; Interleukin-2; NK cell; NKG2D
Categories
Funding
- NIH [DK051362, DK044319, DK053056, DK088199, AI066897]
- Harvard Digestive Diseases Center (NIH) [DK034854]
- Israel-U.S. Binational Research Award
- Canadian Institutes of Health Research
- Crohn's & Colitis Foundation of America
- Deutsche Forschungsgemeinschaft [Ze 814/1-1, OL 424/1-1]
- Crohn's and Colitis Foundation of America
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Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is expressed on activated natural killer (NK) cells wherein it inhibits lysis of CEACAM1-bearing tumor cell lines. The mechanism for this is unknown. Here, we show that interleukin-2-induced expression of CEACAM1 on both mouse and primary human NK cells impairs the ability of NK gene complex group 2 member D (NKG2D) to stimulate cytolysis of CEACAM1-bearing cells. This process requires the expression of CEACAM1 on the NK cells and on the tumor cells, which is consistent with the involvement of trans-homophilic interactions between CEACAM1. Mechanistically, co-engagement of NKG2D and CEACAM1 results in a biochemical association between these two surface receptors and the recruitment of Src homology phosphatase 1 by CEACAM1 that leads to dephosphorylation of the guanine nucleotide exchange factor Vav1 and blockade of downstream signaling that is associated with the initiation of cytolysis. Thus, CEACAM1 on activated NK cells functions as an inhibitory receptor for NKG2D-mediated cytolysis, which has important implications for understanding the means by which CEACAM1 expression adversely affects tumor immunity.
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