Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 39, Issue 7, Pages 1831-1840Publisher
WILEY
DOI: 10.1002/eji.200939309
Keywords
DNA and modified vaccinia virus Ankara vaccines (MVA) Ecotropic HIV-1 mouse challenge model; HIV; HLA-B*5101; T-cell vaccine
Categories
Funding
- Japanese Foundation for AIDS Prevention
- US PHS [RAI-79702, R01DA17618]
- Royal Thai Government Scholarship
- Darwin and Clarendon D.Phil. Scholarhips
- MRC [MC_U137884179] Funding Source: UKRI
- Medical Research Council [MC_U137884179] Funding Source: researchfish
Ask authors/readers for more resources
Novel candidate HIV-1 vaccines have been constructed, which are tailor-designed for HLA-B*5101(+) patients infected with HIV-1 clade B. These vaccines employ novel immunogen HIVB-B*5101 derived from consensus HIV-1 clade B Gag p17 and p24 regions coupled to two Pol-derived B*5101-restricted epitopes, which are together with a third B*5101 epitope in Gag dominant in HIV-1-infected long-term non-progressing patients. Both plasmid DNA and modified vaccinia virus Ankara (MVA) vectors supported high expression levels of the HIVB-B*5101 immunogen in cultured cells. Heterologous DNA prime-recombinant MVA boost regimen induced efficiently HIV-1-specific CD8(+) T-cell responses in BALB/c mice. These vaccine-elicited T cells were multifunctional, killed efficiently target cells in Vim, and protected mice against challenge with ecotropic HIV-1/NL4-3 and ecotropic HIV-1/NDK chimaeric viruses with HIV-1 clade B or D backbones, respectively, and ecotropic murine leukemia virus gp80 envelope, and therefore did so in the absence of anti-HIV-1 gp120 antibodies. These results support further development of HIVB-B*5101 vaccines in combined heterologous-modality regimens. The use of allele-specific vaccines in humans is discussed in the context of other developments in the HIV-1 field.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available