Article
Microbiology
Yiwei Zhu, Jie Chen, Han Shen, Zhongju Chen, Qi-Wen Yang, Jin Zhu, Xi Li, Qing Yang, Feng Zhao, Jingshu Ji, Heng Cai, Yue Li, Linghong Zhang, Sebastian Leptihn, Xiaoting Hua, Yunsong Yu
Summary: Klebsiella pneumoniae carbapenemase (KPC)-producing Pseudomonas aeruginosa (KPC-PA) strains were found in 40.4% of carbapenem-resistant P. aeruginosa (CRPA) isolates, with half of them resistant to ceftazidime-avibactam (CAZ-AVI). Eight plasmid types and two mobile genetic elements mediating bla(KPC-2) transmission were identified through sequencing. Curing bla(KPC-2) plasmids in 28 strains restored CAZ-AVI susceptibility, indicating its role in resistance.
Article
Immunology
Xuefei Zhang, Leilei Wang, Dan Li, Pei Li, Lili Yuan, Fan Yang, Qinglan Guo, Minggui Wang
Summary: This study investigated the distribution and dissemination mechanism of bla (IMP-45) in clinical carbapenem-resistant Pseudomonas aeruginosa isolates in China. Seventeen IMP-45-producing strains were identified, with an outbreak of 11 strains in the ICU of a hospital. Genomic comparison revealed that bla (IMP-45)-carrying plasmids had a similar backbone structure to IncP-2 bla (IMP-9)-carrying plasmids but lacked the repA-oriV-parAB region.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Microbiology
Haoyu Ge, Jie Qiao, Jiahao Zheng, Hao Xu, Ruishan Liu, Junhui Zhao, Ruyan Chen, Chenyu Li, Xiaobing Guo, Beiwen Zheng
Summary: This study investigated the occurrence, antibiotic resistance profiles, and plasmid characteristics of Pseudomonas aeruginosa carrying bla(KPC-3) in China. Five strains carrying bla(KPC-3) were isolated from two patients without a history of travel. These strains belonged to a new sequence type (ST1076) and carried a 395-kb IncP-2 megaplasmid with a conserved genetic structure. The findings highlight the importance of continuous monitoring and control of bla(KPC-3) dissemination.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Infectious Diseases
Areti Tychala, Georgios Meletis, Paraskevi Mantzana, Angeliki Kassomenaki, Charikleia Katsanou, Aikaterini Daviti, Lydia Kouroudi, Lemonia Skoura, Efthymia Protonotariou
Summary: The prompt detection of carbapenemases among Gram-negative bacteria is vital for infection control. This study evaluated a new immunochromatographic assay for detecting carbapenemases and found it to be highly effective and faster than traditional methods. The assay detected more carbapenemases than the standard test, especially in certain types of bacteria.
Article
Biochemistry & Molecular Biology
Jovana Kabic, Gianuario Fortunato, Ivone Vaz-Moreira, Dusan Kekic, Milos Jovicevic, Jovan Pesovic, Lazar Ranin, Natasa Opavski, Celia M. Manaia, Ina Gajic
Summary: This nationwide study aimed to investigate the molecular characteristics, resistance mechanisms, genetic context, and clonal relationship of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa in Serbia. The study found that 320 out of 5334 isolates collected from 2018 to 2021 were identified as P. aeruginosa, and 43.1% of carbapenem-resistant isolates carried the bla(NDM-1) gene. The study also revealed the presence of high-risk clones ST235 and ST654, which are important vectors of MBL and ESBL and are associated with multidrug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Katja Kehl, Anja Schallenberg, Christiane Szekat, Cathrin Albert, Esther Sib, Martin Exner, Nicole Zacharias, Christiane Schreiber, Marjio Parcina, Gabriele Bierbaum
Summary: The study identified carbapenem-resistant bacteria in the wastewater of a large hospital, with high-risk clones potentially being directly disseminated into the environment. Two possible transmission pathways were identified, highlighting the need for remediation measures at both ends of the wastewater system.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Parasitology
Wei Feng, Jun Lv, Hongping Wang, Pu Yao, Lirong Xiong, Peiyuan Xia, Qian Yuan, Fengjun Sun
Summary: This study is the first to determine the complete sequence of a bla(IMP-10)-carrying plasmid and report a bla(IMP-10)-producing strain in China. The plasmid has the same backbone as the one carrying bla(IMP-1) but different accessory resistance regions.
Review
Infectious Diseases
Ahmad Ayman Dabbousi, Fouad Dabboussi, Monzer Hamze, Marwan Osman, Issmat I. Kassem
Summary: This study investigated the epidemiology and antimicrobial susceptibility of multidrug-resistant Pseudomonas aeruginosa in Lebanon. The bacterium was found to be predominant in lesions of patients on mechanical ventilation, burn patients, and those with diabetic foot infections and hematological malignancies. Additionally, carbapenem-resistant P. aeruginosa was detected in animals, with a recent emergence in livestock. However, no studies evaluated the contribution of multidrug-resistant P. aeruginosa in the environment to human infections.
Article
Infectious Diseases
Miki Takemura, Mark G. Wise, Meredith A. Hackel, Daniel F. Sahm, Yoshinori Yamano
Summary: This study evaluated the in vitro antibacterial activity of siderophore cephalosporin cefiderocol against MBL-producing clinical isolates. The results showed that cefiderocol exhibited potent in vitro activity against MBL-producing strains of different bacterial species.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Immunology
Hangbin Hu, Yan Zhang, Piaopiao Zhang, Jie Wang, Qing Yuan, Weixiao Shi, Sheng Zhang, Haiting Feng, Yunbo Chen, Meihong Yu, Hongchao Chen, Yan Jiang, Qing Yang, Tingting Qu
Summary: The study found that KPC-producing CRPA belonging to Sequence Type 463 had a significant impact on bloodstream infections in East China, with a higher mortality rate and resistance to antimicrobials compared to non-ST463 strains. The ST463 CRPA clone showed a high virulence in a larvae model and harbored the bla(KPC) and exoU/exoS genes, indicating a potential new threat in clinical settings. Surveillance of this high-risk clone should be strengthened in China and globally in the future.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Kamrul Islam, Fekade B. Sime, Steven C. Wallis, Michelle J. Bauer, Brian M. Forde, Patrick Harris, Tahmina Shirin, Zakir H. Habib, Meerjady S. Flora, Jason A. Roberts
Summary: This study compared the pharmacodynamics of piperacillin/tazobactam and meropenem against ESBL-producing and non-producing E. coli. The results suggest that piperacillin/tazobactam may be a potential alternative to carbapenems in treating urosepsis caused by ESBL-producing E. coli.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Agronomy
Gaber Attia Abo-Zaid, Ahmed Salah Abdullah, Nadia Abdel-Mohsen Soliman, Ebaa Ebrahim El-Sharouny, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek, Soraya Abdel-Fattah Sabry
Summary: The secretion of siderophores by Pseudomonas aeruginosa F2 and P. fluorescens JY3 was evaluated and found to have an inhibitory effect on Fusarium oxysporum and Rhizoctonia solani. Two optimized media were used to produce siderophores as biocontrol agents, and the cell-free supernatants containing siderophores were used to prepare bio-friendly formulations. These formulations showed significant reduction in damping-off caused by F. oxysporum and R. solani, as well as promoting the growth of wheat plants.
Article
Immunology
Biying Zhang, Xun Xu, Xiaomei Song, Yicheng Wen, Zhichen Zhu, Jingnan Lv, Xiaofang Xie, Liang Chen, Yi-Wei Tang, Hong Du
Summary: The study revealed the shift of CRPA from ST697 to ST463 in East China, with ST463 showing higher drug resistance, posing greater challenges for clinical management.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Microbiology
Sai Rama Sridatta Prakki, Pei Yun Hon, Ze Qin Lim, Natascha May Thevasagayam, Song Qi Dennis Loy, Partha Pratim De, Kalisvar Marimuthu, Shawn Vasoo, Oon Tek Ng
Summary: This study identified the presence of carbapenemase genes bla(IMP) and bla(VIM) in Pseudomonas aeruginosa ST308 clone in clinical and environmental samples from a tertiary-care hospital in Singapore. Whole-genome sequencing revealed the introduction of bla(NDM-1)-Positive ST308 isolates in Singapore more than a decade ago. Genetic analysis showed close genetic relatedness and possible clonal transmission of bla(NDM-1)-positive ST308 isolates between different hospitals.
MICROBIOLOGY SPECTRUM
(2023)