Review
Infectious Diseases
Zineb Cherak, Lotfi Loucif, Abdelhamid Moussi, Jean-Marc Rolain
Summary: Carbapenem resistance in Gram-negative bacteria is a major public health challenge, with transmission mainly occurring through water environments. Aquatic environments serve as a significant medium for antibiotic gene transfer, playing a crucial role in the spread of drug resistance in the environment and community.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Abdurezak Zakir, Belayneh Regasa Dadi, Addis Aklilu, Yisiak Oumer
Summary: This study found a high prevalence of ES beta L-producing gram-negative bacilli among neonates, with a significant association between rectal carriage of ES beta L and factors such as endotracheal intubation, specific antibiotic treatments, and length of stay in the neonatal intensive care unit. Continuous monitoring of drug resistance patterns is crucial for effective treatment and management of these bacteria.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Mariam Rima, Saoussen Oueslati, Laura Dabos, Dina Daaboul, Hassan Mallat, Elie Bou Raad, Marcel Achkar, Osman Mawlawi, Sandrine Bernabeu, Remy A. Bonnin, Delphine Girlich, Marwan Osman, Monzer Hamze, Thierry Naas
Summary: This study investigated the prevalence of carbapenem-resistant Gram-negative bacilli (CR-GNB) in three hospitals in northern Lebanon between 2015 and 2019. The findings highlighted a concerning situation of carbapenem resistance and emphasized the need for effective registration systems in healthcare centers to limit resistance spread.
Article
Infectious Diseases
Ayan Ali Ragueh, Mohamed Houmed Aboubaker, Sitani Idriss Mohamed, Jean-Marc Rolain, Seydina M. Diene
Summary: This study investigated the resistance rate to carbapenems among multidrug-resistant Gram-negative bacteria (MDR-GNB) in Djibouti and characterized their resistance mechanisms. The results showed that out of 256 isolates, 20 were resistant to carbapenems. Carbapenemase genes such as bla(OXA-48) and bla(NDM-5) were identified in the isolates.
Article
Microbiology
Kai Saito, Sayaka Mizuno, Ryuichi Nakano, Ayako Tanouchi, Tomoki Mizuno, Akiyo Nakano, Yuki Suzuki, Naoki Kakuta, Hisakazu Yano
Summary: Carbapenemase-producing Enterobacterales (CPE) are one of the most serious antimicrobial resistance threats to public health worldwide. Rapid detection of CPE infection is crucial for patient management. The NG-Test CARBA 5, an immunochromatographic assay, demonstrates high sensitivity and specificity for detecting the five most common carbapenemase producers.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Microbiology
Ines Rezzoug, Cecile Emeraud, Aimie Sauvadet, Garance Cotellon, Thierry Naas, Laurent Dortet
Summary: The MAST Carba PAcE test showed high specificity but lower sensitivity in detecting carbapenemase-producing Gram-negative bacilli. Interpretation difficulties, especially with OXA-48-like enzymes, were the main reason for the lower sensitivity of the test.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Carmen Antonia Sanches Ito, Larissa Bail, Lavinia Nery Villa Stangler Arend, Kleber Oliveira Silva, Simone Sebold Michelotto, Keite da Silva Nogueira, Felipe Francisco Tuon
Summary: The study evaluated the performance of a new MicroScan Neg multidrug-resistant MIC 1 (NMR1) panel for clinical carbapenem-nonsusceptible Gram-negative bacilli isolates, demonstrating high reliability in detecting ceftazidime-avibactam and ceftolozane-tazobactam. The panel is a viable option for clinical applications, especially for KPC-producing Enterobacterales and carbapenem-nonsusceptible P. aeruginosa.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Vincent Plassard, Philippe Gisbert, Sophie A. Granier, Yves Millemann
Summary: The study aimed to test a surveillance protocol for detecting specific bacteria in dairy farms, with limitations including missing samples and lack of specificity in detection results.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Public, Environmental & Occupational Health
Gunasekaran Rameshkumar, Ranjithkumar Dhandapani, Prajna Lalitha, Siva Ganesa Karthikeyan Rajapandian, Velmurugan Palanivel, Sathiamoorthi Thangavelu, Abdullah A. Alyousef, Thamer Albalawi, Pravej Alam, Mohammad Zubair, Fayez M. Saleh, Fuad Abdullah Alatawi, Fohad M. Husain
Summary: In this study, the prevalence, antibacterial sensitivity patterns, and molecular characterization of MβL associated resistant genes in gram-negative bacteria isolated from ocular infections were investigated. The study found a high prevalence of MβL production in Pseudomonas aeruginosa, leading to high resistance to certain antibiotics. The bla(VIM) gene was also detected at a high rate in eye infections.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Elisavet Stavropoulou, Chrysoula (Chrysa) Voidarou, Georgios Rozos, Natalia Vaou, Michael Bardanis, Theodoros Konstantinidis, Georgia Vrioni, Athanasios Tsakris
Summary: The antibacterial activity and physicochemical parameters of Greek honeys against multidrug-resistant Gram-negative pathogens were evaluated. Different honeys showed significant differences in physicochemical parameters, and the antibacterial activity depended on the type of honey, its dilution, treatment methodology, and microorganism. Some honeys exhibited higher antibacterial potency than manuka honey.
Article
Medicine, General & Internal
Yong Kyun Kim, In Bok Chang, Han Sung Kim, Wonkeun Song, Seung Soon Lee
Summary: The study found that OXA-48-like and Klebsiella pneumoniae are associated with prolonged carriage of CPE. Additionally, patients with CP-K. pneumoniae, positive clinical specimen of CPE, and concurrent Clostridioides difficile infection are more likely to have prolonged carriage of CPE.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Infectious Diseases
Soufiane Sanou, Abdoul Salam Ouedraogo, Salim Aberkane, Julie Vendrell, Oumar Ouchar, Nicolas Bouzimbi, Arsene Hema, Armel Poda, Jacques Zoungrana, Georges A. Ouedraogo, Christian Carriere, Helene Jean-Pierre, Rasmata Ouedraogo Traore, Sylvain Godreuil
Summary: The study confirmed the presence of carbapenemase-producing gram-negative bacilli (GNB) in samples from patients and healthy volunteers, indicating the need for more effective active surveillance activities.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Phatsarawadee Hemwaranon, Arpasiri Srisrattakarn, Aroonlug Lulitanond, Patcharaporn Tippayawat, Ratree Tavichakorntrakool, Lumyai Wonglakorn, Jureerut Daduang, Aroonwadee Chanawong
Summary: Carbapenem-resistant Enterobacterales (CRE) with carbapenemases, especially OXA-48 group, are spreading rapidly and posing a global public health concern. The current phenotypic detection method for OXA-48-like carbapenemases is not optimal, while the PCR-based methods are time-consuming. In this study, a recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip assay was developed for the rapid detection of bla(OXA-48-like) genes in Enterobacterales. The assay showed excellent performance in sensitivity and specificity and had a detection limit 100 times lower than the PCR method.
Article
Microbiology
Claudia Stein, Isabel Lange, Juergen Roedel, Mathias W. Pletz, Frank Kipp
Summary: The integrative method described in this study allows for the detection of carbapenemase-producing Gram-negative bacteria on surfaces and fomites in patient environments. By using Polywipes(TM) to sample surfaces and specific qPCR primers for detection, the spread of these bacteria within patient rooms can be effectively tracked.
Editorial Material
Microbiology
Sanchita Das, Karen Bush
Summary: The emergence and spread of antimicrobial resistance, especially in Gram-negative bacteria, have led to significant morbidity and increased healthcare costs. Large surveillance studies, such as the one performed by the Antibiotic Resistance Laboratory Network, are immensely valuable in understanding the extent of resistance mechanisms, particularly among carbapenemase-producing Gram-negative bacteria. However, routine laboratory detection of carbapenemases in these bacteria remains challenging and requires further optimization.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Letter
Respiratory System
Quentin Menetrey, Fabien Aujoulat, Raphael Chiron, Estelle Jumas-Bilak, Helene Marchandin, Chloe Dupont
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Biochemistry & Molecular Biology
Sonia Andre, Morgane Picard, Renaud Cezar, Florence Roux-Dalvai, Aurelie Alleaume-Butaux, Calaiselvy Soundaramourty, Andre Santa Cruz, Ana Mendes-Frias, Clarisse Gotti, Mickael Leclercq, Alexandre Nicolas, Alexandra Tauzin, Alexandre Carvalho, Carlos Capela, Jorge Pedrosa, Antonio Gil Castro, Lucy Kundura, Paul Loubet, Albert Sotto, Laurent Muller, Jean-Yves Lefrant, Claire Roger, Pierre-Geraud Claret, Sandra Duvnjak, Tu-Anh Tran, Gina Racine, Ouafa Zghidi-Abouzid, Pierre Nioche, Ricardo Silvestre, Arnaud Droit, Fabrizio Mammano, Pierre Corbeau, Jerome Estaquier
Summary: By comparing COVID-19 patients with healthy donors, the study found a strong correlation between T cell apoptosis and lymphopenia in severe cases. The plasma levels of soluble FasL (sFasL) and T cell surface expression of Fas/CD95 were positively correlated with T cell death and CD4 T cell counts. The study also identified modulations in the Bcl-2 family and demonstrated the potential of a pan-caspase inhibitor, Q-VD, to prevent T cell apoptosis and enhance Th1 transcripts.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Medicine, General & Internal
Jan Holubar, Jonathan Broner, Erik Arnaud, Olivier Halle, Thibault Mura, Benjamin Chambert, Albert Sotto, Camille Roubille, Cecile Gaujoux-Viala, Radjiv Goulabchand
Summary: Among 317 IUO patients, noninfectious inflammatory diseases were the major diagnoses, and F-18-FDG-PET/CT was found to be helpful in the diagnostic strategy. Polymyalgia rheumatica and giant cell arteritis were the top causes of noninfectious inflammatory diseases.
JOURNAL OF INTERNAL MEDICINE
(2022)
Letter
Infectious Diseases
Romaric Larcher, Paul Laffont-Lozes, Paul Loubet, Didier Laureillard, Tayma Naciri, Albert Sotto
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Review
Biochemistry & Molecular Biology
Vincent Jean-Pierre, Agathe Boudet, Pauline Sorlin, Quentin Menetrey, Raphael Chiron, Jean-Philippe Lavigne, Helene Marchandin
Summary: Staphylococcus aureus is a major human pathogen that can survive and cause diseases in various clinical settings, including Cystic Fibrosis. In Cystic Fibrosis, S. aureus is the most commonly identified opportunistic pathogen, and its colonization/infection can become chronic in about one third of patients. S. aureus's ability to form biofilm is a crucial factor contributing to its persistence and protection in the lungs of CF patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Cassandra Pouget, Alix Pantel, Catherine Dunyach-Remy, Chloe Magnan, Albert Sotto, Jean-Philippe Lavigne
Summary: Diabetic foot infections are a major public health problem. This study evaluated the in vitro activity of antibiotics against bacteria isolated from diabetic foot infections using a dynamic system. The combination of CWM and the BioFlux(TM) microfluidic system could help clinicians in managing chronic wounds.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Hematology
Jean-Christophe Gris, Florence Guillotin, Taissa Pereira dos Santos, Mathias Chea, Paul Loubet, Didier Laureillard, Albert Sotto, Laurent Muller, Saber Davide Barbar, Claire Roger, Jean-Yves Lefrant, Boris Jung, Kada Klouche, Thibault Mura, Isabelle Quere, Antonia Perez-Martin
Summary: In this study, thrombin generation assays were performed on severe COVID-19 patients, and it was found that these assays could not predict the early or late survival rates of the patients, nor were they associated with thrombotic events. However, by detecting the changes in endogenous thrombin potential (ETP), it was possible to predict whether patients would need to be transferred to an intensive care unit in the future.
THROMBOSIS RESEARCH
(2023)
Article
Immunology
Romaric Larcher, Paul Laffont-Lozes, Claire Roger, Regine Doncesco, Celine Groul-Viaud, Aurelie Martin, Paul Loubet, Jean-Philippe Lavigne, Alix Pantel, Albert Sotto
Summary: This study described the use of last resort beta-lactam antibiotics in hospitalized patients and evaluated the microbiological and clinical outcomes. The results showed that the use of these antibiotics in real-life settings was a safe and efficient therapeutic option for severe infections caused by Gram-negative bacteria with Difficult-to-Treat Resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Koko Barrigah-Benissan, Jerome Ory, Claire Simon, Paul Loubet, Aurelie Martin, Jean-Paul Beregi, Jean-Philippe Lavigne, Albert Sotto, Romaric Larcher
Summary: This study aimed to assess the clinical factors associated with peripherally inserted central catheter (PICC)-related bloodstream infections (PR-BSI), with a focus on clinical symptoms. The results showed that fever and chills were factors associated with the occurrence of PR-BSI.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2023)
Article
Infectious Diseases
Paul Laffont-Lozes, Didier Laureillard, Paul Loubet, Robin Stephan, Myriam Chiaruzzi, Edouard Clemmer, Aurelie Martin, Claire Roger, Laurent Muller, Pierre-Geraud Claret, Radjiv Goulabchand, Clarisse Roux, Jean-Philippe Lavigne, Albert Sotto, Romaric Larcher
Summary: We conducted a prospective cohort study to assess the factors associated with mortality in patients with SARS-CoV-2 pneumonia treated with tocilizumab. Our results showed that age, Charlson index, WHO-CPS, and early administration of tocilizumab were independently associated with mortality. There was no significant difference in mortality between patients treated with tocilizumab and standard of care alone, but better survival was observed in patients who received tocilizumab within 48 hours of hospital admission.
Article
Infectious Diseases
Tayma Naciri, Boris Monnin, Alix Pantel, Claire Roger, Jean-Marie Kinowski, Paul Loubet, Jean-Philippe Lavigne, Albert Sotto, Romaric Larcher
Summary: This study compared the outcomes of beta-lactam allergic (BLA) patients and non-beta-lactam allergic (NBLA) patients with intra-abdominal infections (IAI). The results showed that BLA patients treated without beta-lactam had the same treatment outcomes as NBLA patients treated with beta-lactam.
Article
Infectious Diseases
Jerome Ory, Quang Le Minh, Hung Phan Tien, Vinh Vu Hai, Elodie Careno, Tatiana Price, Alexandre Andrieux, Julien Crouzet, Catherine Dunyach-Remy, Didier Laureillard, Jean-Philippe Lavigne, Albert Sotto
Summary: This study examined the changes in surgical site infection rates before and after the implementation of antibiotic stewardship programs and infection control measures in a Vietnamese tertiary care hospital. The results showed a decrease in surgical site infection rates after the implementation of these programs, although the difference was not statistically significant. Comprehensive infection control education and policies are needed in Vietnamese hospitals to prevent surgical site infections.
Article
Infectious Diseases
Helene Marchandin, Cyril Anjou, Gaetan Poulen, Jane Freeman, Mark Wilcox, Helene Jean-Pierre, Frederic Barbut
Summary: This study reported a case of fidaxomicin-resistant C. difficile infection in a patient treated with fidaxomicin, characterized the genetic determinant for resistance and its effects on pathophysiological traits, and reviewed relevant literature. The observation supports the need for prospective surveillance of C. difficile susceptibility to treatment antibiotics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Aurelie Martin, Paul Loubet, Florian Salipante, Paul Laffont-Lozes, Julien Mazet, Jean-Philippe Lavigne, Nicolas Cellier, Albert Sotto, Romaric Larcher
Summary: This study describes the clinical characteristics and treatment outcomes of patients with enterococcal bone and joint infections and identifies factors associated with treatment failure. The study found that enterococcal BJIs have poor prognosis, with a treatment failure rate of 39%. Coinfection with Staphylococcus epidermidis and the presence of local signs of inflammation at the time of diagnosis were identified as risk factors for treatment failure.
Article
Urology & Nephrology
Franck Bruyere, Yoann Conan, Maxime Vallee, Christophe Gaborit, Hugo Crespin, Joseph-Alain Ruimy, Albert Sotto, Leslie Grammatico-Guillon
Summary: This study identified age and uropathy as independent predictors of abnormal ultrasound in women treated with community-onset acute pyelonephritis.
WORLD JOURNAL OF UROLOGY
(2022)