Journal
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
Volume 32, Issue 6, Pages 781-786Publisher
SPRINGER
DOI: 10.1007/s10096-012-1808-2
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Funding
- Fundacao para a Ciencia e Tecnologia [SFRH/BPD/74480/2010, PIC/IC/82815/2007]
- European Commission [TD1004]
- Fundação para a Ciência e a Tecnologia [PIC/IC/82815/2007] Funding Source: FCT
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We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
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