Article
Public, Environmental & Occupational Health
Ibrahim Yildiz, Zeynep Erdem Aynur
Summary: This study determined for the first time that cattle are suitable hosts for D. fragilis, and the detected parasite belongs to genotype 1, which is the most common type in humans, suggesting a possible zoonotic character.
ZOONOSES AND PUBLIC HEALTH
(2022)
Article
Food Science & Technology
Christian Brenn, Ute Schroeder, Reinhold Hanel, Pedro Martinez Arbizu
Summary: The study presents a TaqManprobe based multiplex real-time PCR screening assay for identification of four commercially relevant crustacean species, showing high specificity and potential for rapid species identification.
Article
Infectious Diseases
Ana Paula Oliveira-Arbex, Erica Boarato David, Simone Mario Caccio, Catia Regina Branco da Fonseca, Joelma Goncalves Martin, Cilmery Suemi Kurokawa, Fabio Tosini, Jayme Augusto Souza-Neto, Semiramis Guimaraes
Summary: This study found that asymptomatic children can carry Dientamoeba in communities where environmental conditions facilitate parasite transmission, and that there is an association between Dientamoeba infection in young children and coinfection with other enteric parasites.
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO
(2021)
Article
Public, Environmental & Occupational Health
Simon Kok Jensen, Uffe Heide-Jorgensen, Soren Viborg Vestergaard, Henrik Toft Sorensen, Christian Fynbo Christiansen
Summary: Creatinine data are crucial in identifying kidney disease, and Denmark has been collecting these data regionally since the 1990s. This study provides information on the geographical coverage of the databases and characteristics of Danish individuals with creatinine tests, which is essential for conducting studies on kidney disease epidemiology.
CLINICAL EPIDEMIOLOGY
(2022)
Article
Medicine, General & Internal
Julian Herrera-Mejia, Rocio Campos-Vega, Abraham Wall-Medrano, Florinda Jimenez-Vega
Summary: Many neurological disorders, especially autism spectrum disorders (ASD), have distinct colonic microbiome signatures. Previous knowledge on this matter was obtained through high-throughput sequencing methods. This study used a sensitive two-step PCR protocol to differentiate colonic microbiome biomarkers in stool samples from ASD and neurotypical individuals.
Article
Pathology
B. S. W. Chong, K. J. Kennedy
Summary: This study compared the accuracy and turnaround time of the BioFire FilmArray Meningitis/Encephalitis multiplex PCR panel to traditional testing algorithms for the diagnosis of meningitis-encephalitis. The ME panel showed high concordance with traditional testing, streamlined the laboratory workflow, and significantly reduced turnaround time. However, the failure of the ME panel to detect Cryptococcus spp. suggests the need for additional testing methods in cases where cryptococcal meningitis is suspected. Despite molecular assays targeting common causes of CNS infection, the diagnostic yield remains suboptimal.
Article
Public, Environmental & Occupational Health
V Caramello, A. Macciotta, A. De Salve, A. Mussa, A. M. De Leo, F. Bar, D. Panno, F. Nota, C. Y. G. Ling, F. Solitro, F. Ricceri, C. Sacerdote, A. Boccuzzi
Summary: This study aimed to compare patients who initially tested negative for COVID-19 with those who tested positive in terms of clinical presentation, laboratory results, imaging findings, and prognosis. Results showed that patients with negative initial tests tended to have more comorbidities such as heart disease, immunosuppression, and diabetes, and imaging was useful in identifying them. True positive patients had a higher risk of serious complications.
Article
Microbiology
Ibne Karim M. Ali, Shantanu Roy
Summary: More than 40 species of the genus Entamoeba exist, with eight infecting humans. A newly developed tetraplex real-time PCR assay can detect and differentiate four morphologically indistinguishable Entamoeba species, offering improved sensitivity and specificity compared to previous assays. This assay could help enhance understanding of the epidemiology and pathogenicity of these species, particularly in low-resource settings.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Betsy Armenta-Leyva, Berenice Munguia-Ramirez, Ting-Yu Cheng, Fangshu Ye, Alexandra Henao-Diaz, Luis G. Gimenez-Lirola, Jeffrey Zimmerman
Summary: This study demonstrates the use of a normalization method in real-time PCR assays to control for normal variation in sampling and testing. The results show that converting sample quantification cycles to efficiency standardized cycles improves the accuracy of the assay. The method is shown to have good diagnostic capability in commercial porcine reproductive and respiratory syndrome virus testing.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Cell Biology
Marte K. Viken, Alice L. Pedersen, Menaka Andersen, Tore Jensen, Benedicte A. Lie, Line M. L. Boulland
Summary: The study established an in-house HLA-B*27 multiplex typing assay using a combination of published, designed, and commercial primers and probes for real-time PCR instruments, enabling quick and large-scale HLA-B*27 typing.
Article
Biochemical Research Methods
Mojca Milavec, Jernej Pavsic, Alexandra Bogozalec Kosir, Gerwyn M. Jones, Denise M. O'Sullivan, Alison S. Devonshire, Fran Van Heuverswyn, Maria Karczmarczyk, Jannika Neeb, Annabell Plauth, Philippe Corbisier, Heinz Schimmel, Andreas Kummrow, Joerg Neukammer, Carole A. Foy, Martin Kammel, Hans-Peter Grunert, Heinz Zeichhardt, Jim F. Huggett
Summary: The study evaluated a candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) in multiple schemes, demonstrating high reproducibility and trueness of the method. The dPCR has the potential to act as a calibration independent reference measurement procedure for value assignment of hCMV calibration and support qPCR calibration.
Article
Multidisciplinary Sciences
Ashley E. Tessnow, Todd M. Gilligan, Eric Burkness, Caroline Placidi De Bortoli, Juan Luis Jurat-Fuentes, Patrick Porter, Danielle Sekula, Gregory A. Sword
Summary: The fall armyworm, Spodoptera frugiperda, is a polyphagous global pest that preferentially feeds on gramineous crops such as corn, sorghum, and pasture grasses. It consists of two genetically distinct host strains known as the corn and rice strains, complicating pest management approaches. Through molecular markers and TaqMan assays, researchers are able to differentiate between strains and interstrain hybrids, allowing for future studies on strain population dynamics and hybrids. Understanding the ecology of S. frugiperda strains is crucial for informing effective management strategies.
Article
Infectious Diseases
Arabella Touati, Cecile Laurier-Nadalie, Cecile Bebear, Olivia Peuchant, Bertille de Barbeyrac
Summary: The study evaluated the performance of four commercial real-time PCR kits for LGV diagnosis and showed that they all demonstrated very good performance for LGV detection in anorectal specimens. The clinical sensitivities and specificities varied between 91% and 100%, with some kits performing better than others. Overall agreement ranged between 91.3% and 100%.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Medicine, General & Internal
Seri Jeong, Nuri Lee, Su Kyung Lee, Eun-Jung Cho, Jungwon Hyun, Min-Jeong Park, Wonkeun Song, Hyun Soo Kim
Summary: This study evaluated the performance of two rapid RT-qPCR assays in detecting SARS-CoV-2, and found that they showed high agreement with conventional RT-qPCR assays and could detect different variants. The STANDARD M10 assay had a shorter turnaround time and random-access detection on automated devices, making it suitable for efficient testing in emergency settings.
Article
Multidisciplinary Sciences
Barbara Suchonska, Wanda Gajzlerska-Majewska, Miroslaw Wielgos
Summary: The real-time optoelectronic method, such as TruScreen, may be useful as an effective tool for cervical cancer screening in developing countries, possibly in combination with other methods (such as cytology), which may contribute to better primary screening.
Article
Infectious Diseases
Roya Salehi, Ali Rostami, Hamed Mirjalali, Christen Rune Stensvold, Ali Haghighi
Summary: The study in Iran found multiple Blastocystis subtypes in farm animals, with ST1 and ST3 present in both human and non-human hosts, and ST2, ST5, ST6, and ST10 found exclusively in specific groups.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Daniela Michlmayr, Luis Alves de Sousa, Luise Muller, Pikka Jokelainen, Steen Ethelberg, Lasse Skafte Vestergaard, Susanne Schjorring, Sarah Mikkelsen, Carl Widstrup Jensen, Lasse Dam Rasmussen, Christen Rune Stensvold
Summary: Enterocytozoon bieneusi was identified as the cause of a foodborne outbreak of diarrhea affecting >70 individuals. The outbreak was associated with consuming food from a workplace canteen, with symptoms including diarrhea, fatigue, and abdominal pain. The study highlights the importance of investigating E. bieneusi in foodborne outbreak investigations.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Morten Frisch, Jacob Simonsen
Summary: In the first national cohort study in Denmark, it was found that non-therapeutic male circumcision in infancy or childhood did not provide protection against HIV or other STIs in males up to age 36. In fact, circumcised males had a higher overall STI rate, particularly for anogenital warts and syphilis.
EUROPEAN JOURNAL OF EPIDEMIOLOGY
(2022)
Article
Microbiology
Christen Rune Stensvold, Brede Aksdal Sorland, Rebecca P. K. D. Berg, Lee O'Brien Andersen, Mark van der Giezen, Joanna L. Bowtell, Ayman A. El-Badry, Salem Belkessa, Ozgur Kurt, Henrik Vedel Nielsen
Summary: Using metabarcoding and bioinformatics analyses, differences were identified in stool microbiota diversity between Blastocystis-positive and Blastocystis-negative individuals. Blastocystis carriers showed higher alpha diversity and enrichment of Firmicutes and Bacteroidetes at the phylum level, while non-carriers showed enrichment of Proteobacteria. The presence of certain genera also differed between carriers and non-carriers. Blastocystis-positive stools appeared to have gut microbiomes associated with eubiosis, while Blastocystis-negative stools showed similarities to dysbiosis-related microbiomes.
Article
Microbiology
Christen Rune Stensvold, Kasandra Ascuna-Durand, Amal Chihi, Salem Belkessa, Ozgur Kurt, Ayman El-Badry, Mark van der Giezen, C. Graham Clark
Summary: In this study, the genetic diversity of Entamoeba coli and Entamoeba hartmanni was further investigated, revealing the existence of previously identified subtypes as well as a potentially new subtype. The subtypes showed varying degrees of genetic homogeneity and intrasubtype diversity. Additionally, substantial variation was found in E. hartmanni, but no links were found between subtype and geographical origin.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2023)
Article
Microbiology
Isbene Sanchez, Alejandro Dashti, Pamela C. Koster, Begona Bailo, Nuria Gonzalez, Janire Allende, Christen Rune Stensvold, David Carmena, David Gonzalez-Barrio
Summary: The enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis, and Dientamoeba fragilis contribute to gastrointestinal illness in high-income countries, and a new multiplex real-time PCR assay has been developed to detect these pathogens with high sensitivity and specificity.
Article
Hematology
Christina Schjellerup Eickhardt-Dalboge, Anna Caecilia Ingham, Lee O'Brien Andersen, Henrik V. Nielsen, Kurt Fuursted, Christen Rune Stensvold, Morten Kranker Larsen, Lasse Kjaer, Sarah Friis Christensen, Trine Alma Knudsen, Vibe Skov, Christina Ellervik, Lars Ronn Olsen, Hans Carl Hasselbalch, Xiaohui Chen Nielsen, Jens Jorgen Elmer Christensen
Summary: Chronic inflammation is important in the development of PV and gut microbiota is believed to be associated with inflammation. The study found that PV patients have an abnormal gut microbiota compared to healthy controls.
Article
Medicine, General & Internal
Andreas Arnholdt Pedersen, Gitte Nyvang Hartmeyer, Christen Rune Stensvold, Raquel Martin-Iguacel
Summary: This case report presents a patient with S. stercoralis hyperinfection syndrome involving the central nervous system, highlighting the global significance of Strongyloides CNS infection in both endemic and non-endemic regions.
Article
Biochemistry & Molecular Biology
Milan Jirku, Andrea Kasparova, Zuzana Lhotska, Miroslav Obornik, Kristyna Brozova, Klara J. Petrzelkova, Peter Samas, Oldriska Kadlecova, Christen Rune Stensvold, Katerina Jirku
Summary: This study focuses on the epidemiology of Dientamoeba fragilis in humans and animals, and the analysis of samples reveals the prevalence rates and related factors of this protist in healthy individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Rikke Boedker Holmstroem, Emilie Kristine Dahl, Morten Helms, Henrik Vedel Nielsen, Janne Bayer Andersen, Jacob Tveiten Bjerrum, Inge Marie Svane, Eva Ellebaek, Jakob Benedict Seidelin
Summary: This article presents a case of a patient with metastatic melanoma who developed severe immune checkpoint inhibitor (ICI)-induced enterocolitis. The patient did not respond to multiple drugs but achieved complete response after fecal microbiota transplantation and tofacitinib treatment.
BMJ OPEN GASTROENTEROLOGY
(2022)
Article
Gastroenterology & Hepatology
Munkhtsetseg Banzragch, Kemal Sanli, Christen Rune Stensvold, Ozgur Kurt, Sule Ari
Summary: The microbiota composition of colonic cleansing fluid (CCF) collected during colonoscopy has been found to distinguish patients with inflammatory bowel disease (IBD) from healthy controls, suggesting that CCF analysis can be an alternative strategy for early diagnosis and disease progression in IBD biomarker research.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Environmental Sciences
Musamba Banza, Hilary Rutto, Tumisang Seodigeng
Summary: This study examined the kinetics of Copper (II) and Lead (II) leaching in EDTA acid. The impacts of various factors were investigated to improve leaching conditions and assess leaching kinetics. The results showed that increasing temperature, stirring speed, and acid concentration, as well as decreasing solid-liquid ratio and pH, enhanced the leaching of copper (II) and lead (II).
SOIL & SEDIMENT CONTAMINATION
(2023)
Editorial Material
Public, Environmental & Occupational Health
Tine Graakjaer Larsen, Jonas Kahler, Marianne Lebbad, Huma Aftab, Luise Mueller, Steen Ethelberg, Lihua Xiao, Christen Rune Stensvold
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Review
Microbiology
Christina Schjellerup Eickhardt-Dalboge, Anna Cacilia Ingham, Henrik V. Nielsen, Kurt Fuursted, Christen Rune Stensvold, Lee O'Brien Andersen, Morten Kranker Larsen, Lasse Kjaer, Sarah Friis Christensen, Trine Alma Knudsen, Vibe Skov, Christina Ellervik, Lars Ronn Olsen, Hans Carl Hasselbalch, Jens Jorgen Elmer Christensen, Xiaohui Chen Nielsen
Summary: This study investigates the gut microbiota in patients with essential thrombocythemia (ET) and found that they have higher richness and different bacterial composition compared to healthy controls. The relative abundance of certain bacteria within the Firmicutes phylum is lower in ET patients, including an important immunoregulative bacterium Faecalibacterium. These findings suggest a potential role of gut microbiota in immune dysregulation of ET patients.
MICROBIOLOGY SPECTRUM
(2023)