Article
Chemistry, Analytical
Yujie Liu, Hui Liu, Guanliu Yu, Wenbo Sun, Muhammad Aizaz, Guiwen Yang, Lei Chen
Summary: A highly efficient single-tube detection platform based on RPA and CRISPR reaction system has been developed to rapidly and accurately detect MRSA infection. The platform demonstrates satisfactory selectivity and can achieve detection within 30 minutes. It shows outstanding performance in sensitivity, specificity, and resistance identification.
ANALYTICA CHIMICA ACTA
(2023)
Article
Infectious Diseases
Fred C. Tenover, Isabella A. Tickler
Summary: The application of molecular detection methods has greatly improved the outcomes of septic patients with MRSA infections. However, the accuracy of molecular tests may be affected by the presence of other staphylococcal species and genetic mutations. Phenotypic methods should be considered when resolving discrepancies between genotypic and phenotypic results.
Article
Infectious Diseases
Cheng-En Tsai, Chia-Jui Yang, Yu-Chung Chuang, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, Shan-Chwen Chang
Summary: This study aimed to determine the synergistic effects of combining CPT with DAP, VAN, or LNZ against different MRSA strains. The results showed that the CPT-DAP combination exhibited significant synergistic bactericidal effects, while the CPT-VAN and CPT-LNZ combinations did not show synergistic activity. Therefore, DAP may be a better choice for combination therapy with CPT in the treatment of MRSA infections.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Chemistry, Analytical
Zhenzhen Tao, Buhua Wang, Qian Cui, Peng Wang, Boris B. Dzantiev, Yi Wan, Jiajia Wu, Zhiqing Yang
Summary: Antibiotic usage in aquaculture has led to the evolution of antibiotic-resistant bacteria, posing adverse effects on aquatic products, ecosystems, and potentially human health. This study proposed a signal-off Cas14a1-based platform (SOCP) for the detection of MRSA, which showed high specificity and fair sensitivity for mecA gene and MRSA. The SOCP could serve as an alternative tool for the diagnosis of antibiotic-resistant bacteria in aquaculture, food industry, and other fields.
ANALYTICA CHIMICA ACTA
(2023)
Review
Materials Science, Multidisciplinary
Shreya Kanth, Akshatha Nagaraja, Yashoda Malgar Puttaiahgowda
Summary: The current global death rate is threatened by deadly unknown infections and the emergence of multidrug-resistant bacteria. Combatting drug-resistant bacteria, such as MRSA, has become a daunting challenge globally, with polymer synthesis being explored as a significant approach in preventing the spread of infections.
JOURNAL OF MATERIALS SCIENCE
(2021)
Article
Cell Biology
Zhimin Bai, Min Chen, Qiaofa Lin, Ying Ye, Hongmei Fan, Kaizhen Wen, Jianxing Zeng, Donghong Huang, Wenfei Mo, Ying Lei, Zhijun Liao
Summary: By extracting feature vectors from protein sequences and using classification tools, MRSA and MSSA were successfully distinguished. MRSA showed high resistance to penicillin and high pathogenic risk. Cross-infection between different types of MRSA was found in Quanzhou, and MRSA in traditional hospitals exhibited increasingly blurred molecular characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Infectious Diseases
Joshua. B. Parsons, Annette. C. Westgeest, Brian. P. B. Conlon, Vance. G. Fowler
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that can persist in the bloodstream for days despite appropriate antibiotics. Persistent MRSA bacteremia is common and associated with poor clinical outcomes. This review explores the factors related to host-pathogen interaction and discusses the clinical relevance of each element. Treatment options and diagnostic approaches for managing persistent MRSA bacteremia are also discussed.
Article
Engineering, Biomedical
Mahdi Zafari, Mahsa Adibi, Mohsen Chiani, Negin Bolourchi, Seyed Mahmoud Barzi, Mohammad Sadegh Shams Nosrati, Zeinab Bahari, Parisa Shirvani, Kambiz Akbari Noghabi, Mojgan Ebadi, Nazanin Rahimirad, Morvarid Shafiei
Summary: The study aimed to evaluate the anti-biofilm activity of cefazolin-loaded nanoparticles against MRSA isolates, showing promising results in effectively treating biofilm-mediated infections.
BIOMEDICAL MATERIALS
(2021)
Article
Infectious Diseases
Radek Sleha, Vera Radochova, Jiri Malis, Alexander Mikyska, Milan Houska, Karel Krofta, Katerina Bogdanova, Sylva Janovska, Jaroslav Pejchal, Milan Kolar, Pavel Cermak, Pavel Bostik
Summary: Purified beta-acids from hops showed significant antimicrobial effects against MRSA isolates in a porcine model of infected wounds, indicating potential for treating non-responsive nosocomial tissue infections by MRSA.
Article
Public, Environmental & Occupational Health
Feiteng Zhu, Hemu Zhuang, Shujuan Ji, Er Xu, Lingfang Di, Zhengan Wang, Shengnan Jiang, Haiping Wang, Lu Sun, Ping Shen, Yunsong Yu, Yan Chen
Summary: The study found that in households of CA-MRSA infected patients in China, most MRSA isolates belonged to epidemic CA-MRSA clones, while no MRSA was detected in the control group households. The research revealed that the home environment is an important reservoir for MRSA.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Microbiology
Melissa J. Karau, Suzannah M. Schmidt-Malan, Scott A. Cunningham, Jayawant N. Mandrekar, Bobbi S. Pritt, Tiffany R. Keepers, Alisa W. Serio, Surya Chitra, Robin Patel
Summary: Omadacycline, vancomycin, and rifampin, as well as rifampin combination therapies, were evaluated in an experimental rat model of MRSA osteomyelitis. The results showed that rifampin combination therapy was effective in reducing MRSA recovery without developing resistance. The combination of omadacycline and rifampin yielded negative MRSA detection, suggesting its potential as a treatment for osteomyelitis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Medicine, General & Internal
Yanan Li, Zhonglin Shi, Anzhong Hu, Junsheng Cui, Ke Yang, Yong Liu, Guoqing Deng, Cancan Zhu, Ling Zhu
Summary: In this study, a rapid and accurate platform for MRSA detection was developed by integrating recombinase polymerase amplification (RPA) with the Cas12 system. The platform allows specific MRSA detection with high sensitivity and can visualize the results within 20 minutes.
Article
Infectious Diseases
Jian Peng, Biswajit Mishra, Rajamohammed Khader, LewisOscar Felix, Eleftherios Mylonakis
Summary: In this study, a smaller antimicrobial peptide C18 derived from cecropin 4 showed high antibacterial activity against various bacteria and yeasts, with a remarkable effect on drug-resistant Staphylococcus aureus. C18 also exhibited the capability to eliminate persister cells, reduce biofilm adherence, interact with DNA, and down-regulate virulence factor genes. Moreover, C18 showed promising results in a Galleria mellonella model, highlighting its potential as a therapeutic candidate for severe MRSA infections.
Article
Food Science & Technology
Martina Florianova, Kristyna Korena, Helena Juricova
Summary: This study evaluated the prevalence of Staphylococcus aureus in dry-fermented salami and found multidrug-resistant and virulent strains. The source of contamination was identified as raw pork and human carriers. Therefore, maintaining high standards of hygiene and manufacturing practices in food processing is crucial.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Infectious Diseases
Martyna Kasela, Mateusz Ossowski, Ewelina Dzikon, Katarzyna Ignatiuk, Lukasz Wlazlo, Anna Malm
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of infectious diseases in humans and animals, resulting in economic losses in human healthcare and the farming sector. The changing epidemiology of MRSA globally affects animal welfare and public health concerns. Livestock have high rates of MRSA colonization, raising questions about reservoirs and transmission, while companion animals vary in MRSA colonization and infection rates, impacting human health. This article highlights the circulation of animal-associated MRSA (AA-MRSA) in the environment and factors influencing its transmission, as well as successful strategies to limit its spread among animals and humans.