Article
Microbiology
Anna Nowaczek, Marta Dec, Dagmara Stepien-Pysniak, Renata Urban-Chmiel, Agnieszka Marek, Pawel Rozanski
Summary: Wild birds can be carriers of potentially dangerous E. coli strains and vectors for the spread of resistant bacteria and resistance determinants in the environment.
Article
Microbiology
Victoria Ballen, Yaiza Gabasa, Carlos Ratia, Melany Sanchez, Sara Soto
Summary: This study characterized 376 extraintestinal pathogenic E. coli strains collected from hospitals in Catalonia, Spain, between 2016 and 2017. The strains showed high resistance to several antibiotics and varied biofilm formation ability. Various virulence and antimicrobial resistance genes were detected, including the colibactin-encoding genes associated with colorectal cancer. The study also investigated the relationship between colibactin and biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Pouya Reshadi, Fatemeh Heydari, Reza Ghanbarpour, Mahboube Bagheri, Maziar Jajarmi, Mohadese Amiri, Hesam Alizade, Mahdi Askari Badouei, Shademan Sahraei, Nasrin Adib
Summary: The study determined the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid and ceftriaxone. The study also found that 26.15% of the E. coli isolates were multi-drug resistant (MDR).
BMC VETERINARY RESEARCH
(2021)
Article
Microbiology
Yongfeng Yu, Changchun Shao, Xiaowei Gong, Heng Quan, Donghui Liu, Qiwei Chen, Yuefeng Chu
Summary: This study investigated the genetic characteristics and variations of tigecycline-resistant Gram-negative isolates from herbivores in northwest China. Whole genome sequencing revealed multiple drug-resistant genes and mechanisms, as well as novel bacterial types and gene mutations. The findings have significant implications for the control and intervention strategies of antimicrobial resistance.
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Chemistry, Multidisciplinary
Zheng Chen, Hailin Wang
Summary: Researching the impact of DNA methyltransferase on antibiotic toxicity can help address antibiotic resistance issues. Our experiments showed that E. coli strains lacking DNA methyltransferase genes were more sensitive to antibiotics.
Article
Microbiology
Mahdaneh Roshani, Mohammad Taheri, Alireza Goodarzi, Rassoul Yosefimashouf, Leili Shokoohizadeh
Summary: The study evaluated antibiotic resistance patterns, virulence factors, biofilm-forming strength, and genetic linkage of Escherichia coli strains isolated from bloodstream infections of leukemia patients. The results showed that E. coli bloodstream infections were more frequent in male patients than female patients, and acute leukemia patients had a higher frequency of infections. Ampicillin and Amoxicillin-clavulanic acid showed the highest resistance, while Imipenem was identified as a suitable antibiotic. MDR phenotypes were present in 22% of the isolates, and the blaCTX-M gene was the most frequent beta-lactamase gene.
Article
Immunology
Malgorzata Andrzejewska, Katarzyna Grudlewska-Buda, Dorota Spica, Krzysztof Skowron, Malgorzata Cwiklinska-Jurkowska, Malgorzata Szady-Grad, Piotr Indykiewicz, Natalia Wiktorczyk-Kapischke, Jacek J. Klawe
Summary: This study aimed to identify the characteristics of Campylobacter isolated from wild birds and water samples, and assess their resistance to antibiotics. The results showed that Campylobacter had virulent properties and high resistance to tetracycline and fluoroquinolones. The lack of genetic relatedness between strains isolated from water and birds suggests other sources of contamination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
Summary: This study assessed the susceptibility and virulence factor gene frequency of clinical P. aeruginosa strains causing bloodstream infections. The results showed that these strains were resistant to multiple antibiotics and had diverse compositions of virulence factor genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Public, Environmental & Occupational Health
Mabel Kamweli Aworh, Jacob K. P. Kwaga, Rene S. Hendriksen, Emmanuel C. Okolocha, Siddhartha Thakur
Summary: The study conducted in Abuja, Nigeria, showed a high prevalence of MDR E. coli among poultry-workers, poultry, and the poultry farm/LBM environment. Occupational exposure was identified as a risk factor for MDR E. coli among poultry-workers. The emergence of MDR E. coli with novel sequence types indicates potential plasmid-mediated transmission, highlighting the importance of enforcing AMR regulations to prevent further spread along the food chain.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Public, Environmental & Occupational Health
Z. Naziri, J. A. Kilegolan, M. S. Moezzi, A. Derakhshandeh
Summary: The study investigated the genetic relatedness, biofilm formation ability, and biofilm-related genes in UPEC isolated from hospital-and community-acquired UTI patients. Results showed that 99% of UPEC isolates demonstrated in vitro biofilm formation ability, with 27% of isolates being moderate to strong biofilm producers, and only the presence of the sfa/focDE gene was significantly associated with moderate and strong biofilm formation. Furthermore, there were higher genetic similarities among UPEC isolates of inpatients compared to outpatients.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Agriculture, Dairy & Animal Science
Jonathan J. Lopez-Islas, Estela T. Mendez-Olvera, Daniel Martinez-Gomez, Andres M. Lopez-Perez, Libertad Orozco, Gerardo Suzan, Carlos Eslava
Summary: Emerging diseases pose a constant challenge in public health, and wild carnivores can be carriers of atypical strains of pathogenic microorganisms, highlighting the importance of natural environments in studying emerging diseases.
Article
Biotechnology & Applied Microbiology
Indranil Mondal, Debjyoti Bhakat, Goutam Chowdhury, Asis Manna, Sandip Samanta, Alok Kumar Deb, Asish Kumar Mukhopadhyay, Nabendu Sekhar Chatterjee
Summary: ETEC strains with CS6 and elt+est toxin genes co-occurred in 51% of isolates. CS21 was found in most strains with est genes (43%). The presence of EatA was associated with the presence of ST alone or with LT, while CS6-harboring strains showed resistance to Ciprofloxacin. Identifying prevalent virulence determinants in Kolkata, India can aid in developing region-specific ETEC vaccines and understanding antibiotic resistance patterns for appropriate treatment.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Dennis J. Woerde, Krystle L. Reagan, Barbara A. Byrne, Bart C. Weimer, Steven E. Epstein, Cory Schlesener, Bihua C. Huang, Jane E. Sykes
Summary: This study examined ESBL-producing bacterial infections in dogs and cats presenting to a veterinary teaching hospital from 2011-2021. Escherichia coli was the most commonly identified bacterial species, with urinary tract infection being the most common clinical presentation. Multi-drug resistance was present in 90% of ESBL-producing bacterial infections. Based on susceptibility patterns, antimicrobials such as piperacillin-tazobactam, amikacin, and cefoxitin may be alternative antibiotics to the current recommended regimen. Whole genome sequencing of bacteria revealed bla(CTX-M-15) as the most common ESBL gene.
VETERINARY SCIENCES
(2023)
Article
Environmental Sciences
Didrik H. Grevskott, Fatemeh Z. Ghavidel, Cecilie S. Svanevik, Nachiket P. Marathe
Summary: This study analyzed antibiotic resistance profiles and diversity of beta-lactamases in Escherichia coli from city sewage surveillance, identifying clinically important antibiotic resistance genes, various sequence types, and isolates carrying multiple virulence factors. The findings suggest potential health concerns and highlight the need for population-based surveillance of antibiotic resistance.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Microbiology
Rajib Majumder, Brodie Sutcliffe, Phillip W. Taylor, Toni A. Chapman
Article
Microbiology
Tiziana Zingali, Toni A. Chapman, John Webster, Piklu Roy Chowdhury, Steven P. Djordjevic
Article
Microbiology
Tiziana Zingali, Cameron J. Reid, Toni A. Chapman, Daniela Gaio, Michael Liu, Aaron E. Darling, Steven P. Djordjevic
Article
Multidisciplinary Sciences
Rajib Majumder, Brodie Sutcliffe, Phillip W. Taylor, Toni A. Chapman
SCIENTIFIC REPORTS
(2020)
Review
Plant Sciences
P. Dadd-Daigle, K. Kirkby, P. Roy Chowdhury, M. Labbate, Toni A. Chapman
Summary: Verticillium dahliae is a major soil-borne phytopathogen causing Verticillium wilt in many important crops globally, including cotton. In Australia, the billion-dollar cotton industry is increasingly impacted by the disease. Different strains of V. dahliae exhibit varying levels of damage to crops in Australian fields, highlighting the need for ongoing research and management strategies to control the disease.
AUSTRALASIAN PLANT PATHOLOGY
(2021)
Article
Biology
Daniela Gaio, Matthew Z. DeMaere, Kay Anantanawat, Graeme J. Eamens, Michael Liu, Tiziana Zingali, Linda Falconer, Toni A. Chapman, Steven P. Djordjevic, Aaron E. Darling
Summary: This study provides a comprehensive analysis of the pig gut microbiota, focusing on the effects of probiotics and intramuscular antibiotic treatment. The dataset generated can be utilized to explore various ecological questions related to antimicrobial resistance, host-associated microbial communities, and phage dynamics.
Article
Genetics & Heredity
Daniela Gaio, Matthew Z. DeMaere, Kay Anantanawat, Toni A. Chapman, Steven P. Djordjevic, Aaron E. Darling
Summary: By analyzing time-series samples of the pig gut microbiome, it was found that a highly structured developmental program exists in piglet gut microbial communities following weaning, which is robust to interventions. Specific taxonomic 'signatures' and the carbohydrate repertoire of organisms resident in the porcine gut were identified, providing insights for the design of probiotics and prebiotic interventions to modify the piglet gut microbiome.
MICROBIAL GENOMICS
(2021)
Article
Pharmacology & Pharmacy
Svetlina S. Vasileva, Jack Tucker, Dan Siskind, Darryl Eyles
Summary: The gut microbiome may mediate antipsychotic-induced side effects in patients with schizophrenia. Microbiome-focused treatments should be used in combination with standard therapy to improve debilitating drug side effects, enhance quality of life, and potentially improve psychotic symptoms.
EXPERT OPINION ON DRUG SAFETY
(2022)
Article
Microbiology
Rajib Majumder, Phillip W. Taylor, Toni A. Chapman
Summary: The domestication process strongly affects the microbiome of Queensland fruit flies (Qfly). The changes in the microbiome have implications for the functional relationship between Qfly and its microbiome, as well as the performance of domesticated and mass-reared insects for sterile insect technique (SIT) programs.
Article
Plant Sciences
Pearl Dadd-Daigle, Damian Collins, Karen Kirkby, Sharlene Roser, Peter Lonergan, Piklu Roy Chowdhury, Maurizio Labbate, Toni A. Chapman
Summary: This study confirms the existence of VCG2A and VCG1A isolates of Verticillium dahliae in Australia that can cause significant damage to cotton plants. The use of ISSR analysis helps to distinguish these isolates from other population and is important for disease diagnosis and confirmation.
AUSTRALASIAN PLANT PATHOLOGY
(2022)
Editorial Material
Plant Sciences
Mohammad Arif, Robert Czajkowski, Toni A. Chapman
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Microbiology
Kirk Broders, Andrew Aspin, Jordan Bailey, Toni Chapman, Perrine Portier, Bevan S. Weir
Summary: Biological collections play a crucial role in preserving our past and protecting our future. This article focuses on the significance of plant bacterial culture collections in global biosecurity and their role in monitoring plant pathogenic bacteria. It presents five case studies to illustrate the importance of culture collections in different situations and discusses the best practices for microbial preservation and accessioning rates. The article emphasizes the need for plant bacterial culture collections to increase their deposits to be prepared for future emerging pathogens and highlights the importance of preserving valuable bacterial strains for global scientific success and biosecurity.
Article
Microbiology
John Webster, Monica A. Kehoe, Elisse Nogarotto, Linda Falconer, Nerida Jane Donovan, Toni A. Chapman
Summary: Swift and accurate diagnostics are essential for responding to pathogen incursions. Current assays for citrus bacterial canker lack specificity when testing Australian samples, prompting the development of a more specific detection tool. Genomic comparisons and validation against isolates were utilized to develop an improved diagnostic method with 100% corroboration, enhancing the ability for effective identification of citrus bacterial canker.
Article
Plant Sciences
K. Kirkby, J. Webster, B. B. Landa, C. Olivares, S. Roser, L. Falconer, D. Gopurenko, T. A. Chapman
Summary: This study reports for the first time the presence of V. dahliae VCG6 isolates infecting X. occidentale in Australia. Morphological and molecular analysis, as well as fulfilment of Koch's postulates, confirmed V. dahliae as the causal pathogen.
AUSTRALASIAN PLANT DISEASE NOTES
(2022)
Article
Multidisciplinary Sciences
Daniela Gaio, Matthew Z. DeMaere, Kay Anantanawat, Graeme J. Eamens, Linda Falconer, Toni A. Chapman, Steven Djordjevic, Aaron E. Darling
Summary: Currently, little is known about the effects of intramuscular antibiotic treatment on the gut microbiome. This study investigated the effects of two probiotic- and one intramuscularly administered antibiotic treatment on the developing gut microbiome of post-weaning piglets. The results showed that individual host differences, such as breed, litter, and age, were important contributors to variation in the community composition, with host age being the dominant factor in shaping the gut microbiota of piglets after weaning.
