Article
Clinical Neurology
Michael Isfort, Bryan J. McVerry, Lori Shutter, Minji Kim, David Lacomis
Summary: This study surveyed ICU providers to understand their approach to ICU-acquired weakness (ICU-AW) and the utility of electrodiagnostic studies (EDx). The results showed that while ICU providers are familiar with CIM/CIP, they lack a standardized evaluation approach for ICU-AW. Clinical experience was considered the most helpful management tool for ICU-AW.
Article
Medicine, General & Internal
Felix Klawitter, Marie-Christine Oppitz, Nicolai Goettel, Mette M. Berger, Carol Hodgson, Steffen Weber-Carstens, Stefan J. Schaller, Johannes Ehler
Summary: Intensive care unit-acquired weakness (ICU-AW) is a common neuromuscular complication in critically ill patients. A global survey found a significant heterogeneity in the diagnosis, treatment, and prevention of ICU-AW internationally. Participants recognized the need for comprehensive guidelines to manage ICU-AW.
MEDICINA-LITHUANIA
(2022)
Article
Clinical Neurology
Lotte Sahin Levison, Reimar Wernich Thomsen, Henning Andersen
Summary: The study found that within 6 months of GBS hospital admission, the mortality rate was 6.6 times higher compared to the general population of the same age. The mortality rate continued to increase for approximately 4 years following GBS, and then leveled off to a similar long-term mortality rate.
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Review
Rehabilitation
Evelyn S. Qin, Catherine L. Hough, James Andrews, Aaron E. Bunnell
Summary: ICU-acquired weakness (ICUAW) is a common complication in patients with COVID-19, with a prevalence of up to 45.5%. Risk factors for developing ICUAW include baseline health status, sepsis, mechanical ventilation, etc. Establishing postacute rehabilitation programs for COVID-19 survivors is crucial for recovery of function and prevention of long-term impairments.
Article
Infectious Diseases
Rodopi Stamatiou, Anna Vasilaki, Dimitra Tzini, Vasiliki Tsolaki, Konstantina Zacharouli, Maria Ioannou, George Fotakopoulos, Markos Sgantzos, Demosthenes Makris
Summary: Colistin is a last-resort drug for multidrug-resistant infections, but its side effects, such as muscle toxicity, need careful monitoring. Vasoconstrictive drugs are used in intensive care to improve blood flow to vital organs. The study found that colistin treatment led to muscle abnormalities in animals, but this effect was reduced when vasoconstrictive drugs were administered. Colistin's effects on muscle depend on the septic state and the presence of vasoconstriction.
Article
Rehabilitation
Renuka T. Rudra, Dan Lin, Benjamin Miller, Ping Du, Shangming Zhang
Summary: Inpatient rehabilitation has shown significant benefits for patients with ICUAW, leading to increased functional independence, longer rehabilitation length of stay, and higher rates of home discharge. However, patients with ICUAW also have a higher prevalence of medical comorbidities, requiring careful medical management.
Article
Clinical Neurology
Susana Santiago-Perez, Sandra Espinosa-Garcia, Almudena Martinez-Perez, Erika Herraez-Sanchez, Cristian Rizea, Lilia Alejandrina Ruiz-Avila
Summary: This study investigated the neurophysiological findings in critically ill COVID-19 patients with neuromuscular deficits. The results showed that myopathy was the most frequent diagnosis, followed by polyneuropathy and focal neuropathies. The common locations of nerve lesions included the fibular head and elbow level. Neurophysiological examination was found to be useful in accurately diagnosing and selecting treatment measures for neuromuscular complaints in these patients.
JOURNAL OF CLINICAL NEUROPHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Federica Marra, Paola Lunetti, Rosita Curcio, Francesco Massimo Lasorsa, Loredana Capobianco, Vito Porcelli, Vincenza Dolce, Giuseppe Fiermonte, Pasquale Scarcia
Summary: Neuromuscular diseases (NMDs) involve skeletal muscle dysfunction due to genetic mutations, leading to muscle weakness and fatigue. Mitochondrial dysfunction plays a key role in the development of NMDs, with alterations in genes and pathways related to mitochondrial function contributing to the disease progression. Diagnosis and treatment strategies for NMDs focus on identifying genetic mutations and mitochondrial pathways involved in the development of the disease.
Review
Critical Care Medicine
Joo Heung Yoon, Michael R. Pinsky, Gilles Clermont
Summary: This article is one of the selected reviews from the Annual Update in Intensive Care and Emergency Medicine 2022. It presents important research findings in the field, and readers can find other selected articles and more information from the provided link.
Article
Medicine, General & Internal
Hsueh-Wen Hsueh, Wen-Chin Weng, Pi-Chuan Fan, Yin-Hsiu Chien, Feng-Jung Yang, Wang-Tso Lee, Ru-Jen Lin, Wuh-Liang Hwu, Chih-Chao Yang, Ni-Chung Lee
Summary: This single-center study utilized next-generation sequencing to diagnose hereditary neuromuscular diseases. The results revealed a great diversity in hereditary NMDs and highlighted the importance of high-quality clinical and laboratory evaluations. Positive family history had a positive impact on the diagnostic rate.
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2022)
Review
Clinical Neurology
Renato Oliveira, Filipa Ramalho Rocha, Tomas Teodoro, Miguel Oliveira Santos
Summary: The differential diagnosis of acute non-traumatic tetraparesis can be systematized based on the site of defect, including upper motor neuron, peripheral nerve, neuromuscular junction, or muscle. A thorough assessment of present illness history, along with neurological examination and ancillary studies, is crucial for accurate diagnosis and timely treatment of potentially life-threatening conditions presenting with acute tetraparesis.
JOURNAL OF CLINICAL NEUROSCIENCE
(2021)
Article
Genetics & Heredity
Naye Balzan Schneider, Erica Caetano Roos, Ana Lucia Portella Staub, Isabela Possebon Bevilacqua, Ana Carolina de Almeida, Tamie de Camargo Martins, Natalia Bergamelli Ramos, Priscilla Loze, Jonas Alex Morales Saute, Ana Paula Beck da Silva Etges, Carisi Anne Polanczyk
Summary: This study evaluates the annual costs of hospital, home care, and transportation for patients with DMD in Brazil. The results show that home care expenditures account for 92% of the total costs, followed by hospital costs (6%) and transportation costs (2%). Accurate information about costs is crucial for establishing sustainable health policies for rare diseases.
ORPHANET JOURNAL OF RARE DISEASES
(2023)
Review
Medicine, General & Internal
Robert T. Mankowski, Orlando Laitano, Thomas L. Clanton, Scott C. Brakenridge
Summary: Sepsis survivors often experience persistent myopathy characterized by skeletal muscle weakness, atrophy, and the inability to repair damaged myofibers. The origins of this myopathy are likely complex, involving interactions between impaired muscle metabolism and circulating pathogens. Physical inactivity during hospitalization may exacerbate the myopathy, highlighting the potential role of physical rehabilitation in preventing decline in physical function for septic patients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Genetics & Heredity
Magdalena Mroczek, Cheryl Longman, Maria Elena Farrugia, Solange Kapetanovic Garcia, Didem Ardicli, Haluk Topaloglu, Aurelio Hernandez-Lain, Diclehan Orhan, Mehmet Alikasifoglu, Jennifer Duff, Sabine Specht, Kristen Nowak, Gianina Ravenscroft, Katherine Chao, Zaheer Valivullah, Sandra Donkervoort, Dimah Saade, Carsten Bonnemann, Volker Straub, Grace Yoon
Summary: FXR1-related congenital myopathy is an emerging entity with significant phenotypic variability.
JOURNAL OF MEDICAL GENETICS
(2022)
Article
Clinical Neurology
Daniele Velardo, Sara Antognozzi, Martina Rimoldi, Serena Pagliarani, Filippo Cogiamanian, Sergio Barbieri, Stefania Corti, Giacomo Pietro Comi, Dario Ronchi
Summary: Exercise-induced muscle stiffness is the main symptom of Brody disease, which is a recessive genetic muscle disorder caused by ATP2A1 gene mutations. The knowledge about the disease's natural history, genotype-phenotype correlations, and treatment effects is still incomplete, leading to underdiagnosis. This study reports the clinical and genetic characteristics of two siblings with childhood-onset exercise-induced muscle stiffness and identified two ATP2A1 mutations associated with the disease.
FRONTIERS IN NEUROLOGY
(2023)
Article
Chemistry, Medicinal
Tony Froehlich, Christina Mai, Roman P. Bogautdinov, Svetlana N. Morozkina, Alexander G. Shavva, Oliver Friedrich, Daniel F. Gilbert, Svetlana B. Tsogoeva
Article
Materials Science, Biomaterials
Christina Fey, Jana Betz, Corinna Rosenbaum, Dana Kralisch, Martin Vielreicher, Oliver Friedrich, Marco Metzger, Daniela Zdzieblo
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Yang Guo, Anna-Lena Merten, Ulrike Schoeler, Ze-Yan Yu, Jasmina Cvetkovska, Diane Fatkin, Michael P. Feneley, Boris Martinac, Oliver Friedrich
Summary: Cardiac mechano-electric coupling is a complex feedback mechanism that regulates cardiac function through Ca2+ signals in acute and chronic pressure overload scenarios. While the molecular mechanisms of how mechanical forces alter cardiac function remain unclear, mechanosensitive channels are believed to play a significant role in cardiac mechano-electric coupling.
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2021)
Article
Engineering, Biomedical
Abbas Shafiee, Amanda S. Cavalcanti, Navid T. Saidy, Dominik Schneidereit, Oliver Friedrich, Akhilandeshwari Ravichandran, Elena M. De-Juan-Pardo, Dietmar W. Hutmacher
Summary: The biomimetically designed mPCL dressings, combined with human cell seeding, promote wound healing, reduce scar formation, and enhance skin regeneration.
Article
Biochemistry & Molecular Biology
Tom Zimmermann, Lisa A. Gebhardt, Lucas Kreiss, Christin Schneider, Stephanie Arndt, Sigrid Karrer, Oliver Friedrich, Michael J. M. Fischer, Anja-Katrin Bosserhoff
Summary: CAP is a partially ionized gas with reported antitumor effects, but its active components and molecular mechanisms are not fully understood. This study characterized the impact of acidified nitrite on melanoma cells, demonstrating a synergistic effect with acidification leading to strong cytotoxicity. It also highlighted the importance of RNS during CAP treatment and evaluated important molecular mechanisms involved in the cancer cell response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sarah Lemire, Oana-Maria Thoma, Lucas Kreiss, Simon Voelkl, Oliver Friedrich, Markus F. Neurath, Sebastian Schuermann, Maximilian J. Waldner
Summary: The activity of immune cells is a major factor in the progression of inflammatory bowel diseases. This study aims to understand the autofluorescence changes depending on the type and activation state of immune cells. The results show that innate immune cells have significantly increased autofluorescence signals compared to adaptive immune cells. In vitro stimulation also increases autofluorescence signals in adaptive immune cells and macrophages. Cell death leads to a significant decrease in NADH autofluorescence, while FAD signals remain unaffected. These findings demonstrate the value of autofluorescence as a tool to characterize immune cells in different functional states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Nina Simon, Cornelia Voigtlaender, Barbara Kappes, Petra Rohrbach, Oliver Friedrich
Summary: Drug resistance often arises from mutations in solute transporters, which may result in the ability of transporters to actively transport drugs away from their target sites. This study examined the transport characteristics of different strains of Plasmodium falciparum with various pfmdr1 gene mutations. The results showed that the drug-resistant strain Dd2 had the highest transport rates, followed by K1 and the drug-sensitive strain 3D7, which correlated with their gene copy numbers. This assay provides a means to evaluate the likelihood of resistance formation for newly developed drugs in the early stages of drug development.
Article
Chemistry, Multidisciplinary
Matthias Ryma, Hatice Genc, Ali Nadernezhad, Ilona Paulus, Dominik Schneidereit, Oliver Friedrich, Kristina Andelovic, Stefan Lyer, Christoph Alexiou, Iwona Cicha, Juergen Groll
Summary: This study presents a facile and flexible approach for integrating biomimetically branched microvasculature within bulk hydrogels. By creating sacrificial scaffolds using melt electrowriting and placing them in a bioreactor filled with hydrogel precursor solution, physiological-like branchings and channels can be generated. This approach allows for rapid formation of a tight and biologically functional endothelial layer.
ADVANCED MATERIALS
(2022)
Article
Oncology
Philipp Kunze, Lucas Kreiss, Vendula Novosadova, Adriana V. Roehe, Sara Steinmann, Jan Prochazka, Carol I. Geppert, Arndt Hartmann, Sebastian Schuermann, Oliver Friedrich, Regine Schneider-Stock
Summary: In this study, the ECM remodeling potential of colorectal tumor cells was investigated using multiphoton microscopy and the chorioallantoic membrane assay. The results showed that DAPK1 ko tumor cells have a high ECM remodeling potential at the tissue level.
Article
Biochemistry & Molecular Biology
Kai Peter Sommer, Adrian Krolinski, Mohammad Mirkhalaf, Hala Zreiqat, Oliver Friedrich, Martin Vielreicher
Summary: The goal of bone tissue engineering is to build artificial bone tissue that closely resembles human bone and supports its integration into the body. This study used two types of 3D-printed scaffolds to seed and differentiate osteoblastic cells, and tested different assays and imaging methods to monitor these processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Mena Michael, Larisa Kovbasyuk, Paul Ritter, Michael B. B. Reid, Oliver Friedrich, Michael Haug
Summary: An oxidizing redox state has unique effects on muscle contraction. However, our understanding of the elasticity and flexibility of muscle fibers is limited. The MyoRobot platform allows us to investigate the calcium responsiveness of muscle contraction and examine the passive stress-strain behavior. Our findings show that an oxidizing environment leads to decreased force generation and increased passive stress in muscle fibers.
Article
Multidisciplinary Sciences
Charlotte Gineste, Sonia Youhanna, Sabine U. Vorrink, Sara Henriksson, Andres Hernandez, Arthur J. Cheng, Thomas Chaillou, Andreas Buttgereit, Dominik Schneidereit, Oliver Friedrich, Kjell Hultenby, Joseph D. Bruton, Niklas Ivarsson, Linda Sandblad, Volker M. Lauschke, Hakan Westerblad
Summary: This study investigated the effects of extracellular matrix (ECM) disruption on cellular and mitochondrial morphology, gene expression, and Ca2+ handling in skeletal muscle fibers. The results showed that enzymatic dissociation of fibers resulted in myopathic phenotypes, shorter and less branched mitochondria, and prolonged mitochondrial Ca2+ accumulation during repeated contractions. However, this accumulation was concealed with enzymatic dissociation, making it an ambiguous method in studies of native intracellular Ca2+ fluxes.
Article
Biochemistry & Molecular Biology
Anna Rederer, Victoria Rose, Rene Krueger, Linda Schmittutz, Izabela Swierzy, Lena Fischer, Ingo Thievessen, Julian Bauer, Oliver Friedrich, Mario Schiffer, Janina Mueller-Deile
Summary: Signaling-pathway analyses and investigation of gene responses are usually done in 2D monocultures, but glomerular cells grow in 3D and have different interactions. We cultured glomerular cells in both 2D and 3D monocultures and co-cultures to study cell behavior, gene expression, and cell-cell interactions. Our results showed that 3D co-cultures exhibited increased expression of cell-specific markers and extracellular matrix compared to 2D co-cultures. Additionally, using 3D co-cultures confirmed intercellular communication between podocytes and glomerular endothelial cells. These findings question the reliability of 2D monocultures and suggest that glomerular 3D co-cultures are more suitable for studying cell communication, disease modeling, and drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
T. Distler, L. Kretzschmar, D. Schneidereit, S. Girardo, R. Goswami, O. Friedrich, R. Detsch, J. Guck, A. R. Boccaccini, S. Budday
Summary: This study investigates the properties of hydrogels containing cells and microgel beads through complex mechanical analyses. The results show that the stiffness of hydrogels significantly decreases when the cell and bead concentrations exceed certain values. Hydrogels with higher cell concentrations exhibit more pronounced material nonlinearity for larger strains and faster stress relaxation.
BIOMATERIALS SCIENCE
(2021)
Article
Medicine, Research & Experimental
Dominik Schneidereit, Anita Broellochs, Paul Ritter, Lucas Kreiss, Zeinab Mokhtari, Andreas Beilhack, Gerhard Kroenke, Jochen A. Ackermann, Maria Faas, Anika Grueneboom, Sebastian Schuermann, Oliver Friedrich
Summary: Structural remodeling or damage in tissues is often unevenly distributed depending on the location and extent of damaging stimuli. By optimizing different clearing protocols, aqueous solution-based protocols using 2,2'-thiodiethanol (TDE) were found to be advantageous in preserving muscle morphology. Applying TDE clearing allows for the assessment of muscle fiber integrity in 3D and quantification of muscle necrosis.