Article
Parasitology
Nikol Reslova, Lucie Skorpikova, Iveta Angela Kyrianova, Jaroslav Vadlejch, Johan Hoglund, Philip Skuce, Martin Kasny
Summary: The study developed two multiplex real-time PCR assays for rapid and accurate identification of gastrointestinal nematode species in ruminants, as well as semi-quantitative estimation of egg numbers. This approach showed higher sensitivity and specificity compared to traditional methods, improving diagnostic accuracy and allowing for more precise evaluation of anthelmintic efficacy.
PARASITES & VECTORS
(2021)
Article
Genetics & Heredity
Sae Rom Hong, Kyoung-Jin Shin
Summary: This study introduces a multiplex quantitative real-time PCR system named BisQuE, which can simultaneously analyze three key features of BS conversion, including efficiency, recovery, and degradation levels. Six BS conversion kits were tested, with NEBNext kit showing the lowest conversion efficiency but also the lowest degradation level among them.
FRONTIERS IN GENETICS
(2021)
Article
Environmental Sciences
Kousar Banu Hoorzook, Tobias George Barnard
Summary: The aim of this study was to develop a cost-effective method to concentrate bacterial cells directly from water for DNA extraction and PCR amplification. The in-house DNA extraction method showed good DNA recovery and repeatable results compared to the commercial kits.
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Sabina Maria Bruno, Giovanna Blacona, Stefania Lo Cicero, Germana Castelli, Mariarita Virgulti, Giancarlo Testino, Silvia Pierandrei, Andrea Fuso, Giuseppe Cimino, Giampiero Ferraguti, Adriana Eramo, Marco Lucarelli
Summary: In the precision medicine era of cystic fibrosis (CF), accurate quantification of CFTR gene expression is crucial for personalized therapeutic approaches. This paper compares two methods for quantifying CFTR gene expression and discusses their advantages and limitations.
Article
Food Science & Technology
Tingyao Zhu, Xiefei Zhou, Wei Zhang, Yue Wu, Jingjing Yang, Liaoyi Xu, Ming Chen, Weiren Dong, Haisheng Xu
Summary: The study developed multiplex and real-time PCR techniques for detecting adulteration in donkey meat, providing high qualitative and quantitative accuracy, which can be applied for commercial foodstuff adulteration detection.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2021)
Article
Microbiology
Agata Anna Cisek, Iwona Bak, Bozena Cukrowska
Summary: Methanogenic archaea are important but often ignored in studies of human and animal intestinal microbiota. This research focused on improving the quantification of methanogens using qPCR, by refining the protocol and increasing the specificity and sensitivity of the assay. The results showed increased detection range and successful validation parameters, allowing for improved detection and quantification of methanogens in stool samples, such as chicken droppings in this study.
Article
Food Science & Technology
Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang Liu
Summary: This study developed a PCR system to efficiently identify sheep/goat, fox, and murine species using nuclear DNA sequences. Multiple mixed DNA samples were quantitatively analyzed using a real-time PCR method, showing good accuracy and precision. This method provides a powerful technology for combating meat adulteration and protecting consumer health.
Article
Chemistry, Applied
Stephanie C. Filep, Kristina Reid Black, Bryan R. E. Smith, Denise S. Block, Anna Kuklinska-Pijanka, Max Bermingham, Maria A. Oliver, Catherine M. Thorpe, Zachary P. Schuhmacher, Sayeh Agah, Sabina Wuenschmann, Martin D. Chapman
Summary: The aim of this study was to develop a fluorescent multiplex array for simultaneously measuring regulated food allergens. The method used specific allergen protein molecules and microspheres coupled to monoclonal antibodies for allergen detection, and showed good accuracy and sensitivity. This method has applications in risk assessment of food allergies and standardization of therapeutic products.
Article
Multidisciplinary Sciences
Paula Finamore-Araujo, Amanda Faier-Pereira, Carlos Ramon do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy de Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio Cruz Moreira
Summary: Oral transmission of T. cruzi infection has become the most relevant mechanism in Brazil, with contaminated food or beverages being a major source of Chagas disease cases. A molecular method has been developed to detect and quantify T. cruzi DNA in acai berries, with the potential for high sensitivity and fast results to aid in the epidemiology, control, and surveillance of Chagas disease outbreaks.
Article
Plant Sciences
Nicholas LeBlanc, Frank Martin, Vanina Castroagudin, Jo Anne Crouch
Summary: Impatiens downy mildew (IDM) disease is a major constraint on the production of Impatiens walleriana. A hydrolysis-probe-based PCR diagnostic assay was developed for the detection and quantification of the pathogen Plasmopara destructor. The assay specifically targets P. destructor infecting both I. walleriana and I. balsamina. This research is important for disease control and detection in the production of Impatiens walleriana.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki -Hun Jeong
Summary: This study presents a rapid and compact real-time RT-PCR system for decentralized molecular diagnostics. It utilizes plasmonic nucleic acid amplification and quantification, and features an ultrafast plasmonic thermocycler, a disposable plastic-on-metal cartridge, and a compact fluorescence microscope. The system successfully enables 10-minute diagnosis of COVID-19 with high amplification efficiency, classification accuracy, and total percent agreement for clinical diagnostic tests.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki-Hun Jeong
Summary: This study presents an ultrafast plasmonic nucleic acid amplification and real-time quantification system for decentralized molecular diagnostics. The system features ultrafast photothermal cycling, rapid heat transfer, and efficient PCR quantification, along with high-contrast fluorescence microscopic imaging. It enables decentralized point-of-care molecular diagnostic testing in primary care and developing countries.
Article
Biology
Fengyu Zhang, Yipeng Wang, Xiaomeng Wang, Hongjie Dong, Min Chen, Ning Du, Hongwei Wang, Wei Hu, Kundi Zhang, Lichuan Gu
Summary: A new method (RT-IVT) leverages fluorescence quenchers & DNA ligase in FRET and RNA-splinted DNA ligation methods to allow inexpensive, nonradioactive and selective mRNA production quantification. The RT-IVT method can detect mRNA production in unpurified systems in real-time and shows high sensitivity and selectivity. Additionally, it enables multiplexed real-time mRNA quantification.
COMMUNICATIONS BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Paul Dawson, Ahmet Buyukyavuz, Claudia Ionita, Julie Northcutt
Summary: The combination of PCR and DNA extraction techniques can be used to enumerate Campylobacter spp. from poultry gastrointestinal tract samples. The study developed a DNA standard curve for Campylobacter spp. DNA primers and a cell/genomic DNA extraction protocol for complex samples. The results showed that qPCR and standard plate count methods were highly correlated for enumerating Campylobacter spp. in the specified range.
