Article
Microbiology
Angela Brunetti, Kurt Heungens, Jacqueline Hubert, Renaud Ioos, Gian Luca Bianchi, Francesca De Amicis, Anne Chandelier, Sietse Van der Linde, Ana Perez-Sierra, Valeria Gualandri, Maria Rosaria Silletti, Vito Nicola Trisciuzzi, Silvia Rimondi, Tiziana Baschieri, Elio Romano, Valentina Lumia, Marta Luigi, Francesco Faggioli, Massimo Pilotti
Summary: This article describes a Real-Time PCR detection method for Ceratocystis platani, a lethal vascular disease agent of Platanus species. The method was validated in nine European laboratories and showed excellent performance parameters, making it a valuable tool for monitoring the spread of C. platani and investigating the resistance level of Platanus genotypes.
Article
Plant Sciences
Meagan Van Dyk, Christoffel F. J. Spies, Lizel Mostert, Francois Halleen
Summary: This study reveals that pruning debris serves as an inoculum source of P. globosa in established olive orchards in South Africa. It emphasizes the importance of reducing the inoculum sources of this pathogen as an additional management strategy against olive trunk diseases.
Article
Food Science & Technology
D. Cristiano, M. F. Peruzy, M. Aponte, A. Mancusi, Y. T. R. Proroga, F. Capuano, N. Murru
Summary: The study investigated the detection methods for Yersinia enterocolitica in leafy greens, finding that only ddPCR technology could detect the pathogen in leafy greens contaminated at low levels. Additionally, the incubation time and temperature were shown to significantly impact the survival and concentration of Yersinia enterocolitica in vegetables.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Saiful Arefeen Sazed, Mohammad Golam Kibria, Mohammad Shafiul Alam
Summary: The real-time PCR method established in this study efficiently detects and distinguishes five human Plasmodium species with a single protocol, improving sensitivity and specificity. This method can be considered as an alternative to existing molecular diagnosis lineup for malaria in endemic countries.
Article
Agriculture, Multidisciplinary
Vincent Blaschke, Alea Berten, Heike Sprenger, Jutta Zagon, Matthias Winkel
Summary: In this study, highly specific real-time PCR methods were developed for the comprehensive detection of all commercially relevant cephalopod species and the gastropod families Helicidae, Buccinidae, and Muricidae in food matrices. The specificity of the systems was demonstrated by cross-testing over 100 animal and plant species. The robustness and practical applicability of the protocol were confirmed through testing multiple parameters and cooking and autoclaving of samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Virology
Mattia Calzolari, Simone Russo, Katia Marzani, Gastone Dalmonte, Matteo Ricchi, Paolo Bonilauri
Summary: A specific real-time PCR method for Fermo virus has been developed and reported in this study. This method is of great importance for studying the epidemiology of Fermo virus and its potential pathogenicity in humans or animals.
Article
Thermodynamics
Lucas H. H. Pereira, Juliana Pereira, Jerusa S. S. Garcia, Marcello G. G. Trevisan
Summary: The aim of this study was to develop a method for detecting adulterants in Extra Virgin Olive Oil (EVOO) using DSC and PLS. Several binary mixtures were prepared using adulterants such as sunflower, corn, and soybean oils. The samples were analyzed using DSC with controlled cooling and PLS models were constructed based on the data. The results showed that the method was successful in quantifying the adulteration level.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2023)
Article
Multidisciplinary Sciences
Subeen Hong, Seung Mi Lee, Sohee Oh, SoYeon Kim, Young Mi Jung, Sun Min Kim, Chan-Wook Park, Jong Kwan Jun, Byoung Jae Kim, Joong Shin Park
Summary: This study evaluated the performance of a PNA probe-based real-time PCR assay for detecting common aneuploidies. The results showed that the assay had a sensitivity and specificity of 100%. Compared to conventional karyotyping, the PNA probe-based real-time PCR assay was rapid and easy to use.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
M. G. Rizzo, S. Carnazza, L. M. De Plano, D. Franco, M. S. Nicol, V Zammuto, S. Petralia, Giovanna Calabrese, C. Gugliandolo, S. Conoci, S. P. P. Guglielmino
Summary: The study developed a new method for rapid and sensitive detection of bacteria in blood using engineered M13 phages and PCR in a miniaturized silicon microchip, providing a fast and efficient diagnosis approach.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Food Science & Technology
Chang-Hun Choi, Eiseul Kim, Seung-Min Yang, Da-Som Kim, Seung-Man Suh, Ga-Young Lee, Hae-Yeong Kim
Summary: This study evaluated the applicability of a ddPCR assay for detecting lactic acid bacteria and compared its performance characteristics to those of a qPCR. The results showed that ddPCR is more sensitive than qPCR, but it has limitations in quantifying high bacterial concentrations.
Article
Food Science & Technology
Africa Sanchiz, Paulina Sanchez-Enciso, Carmen Cuadrado, Rosario Linacero
Summary: This study used chloroplast markers and real-time PCR to enhance the sensitivity of peanut detection, applying different DNA extraction protocols, selecting appropriate primers and probes for detection, and ultimately determining that the mat k chloroplast marker was the most sensitive. The method is also capable of accurately detecting peanuts in processed foods, with a wide range of potential applications.
Article
Food Science & Technology
Joana S. Amaral, Fatima Z. Raja, Joana Costa, Liliana Grazina, Caterina Villa, Zoubida Charrouf, Isabel Mafra
Summary: In this study, real-time PCR methods were developed to detect adulteration of argan oil with olive and soybean oils. The methods were able to quantify the concentrations of adulterants and showed reliable and high-throughput performance.
Article
Food Science & Technology
Vincent M. Blaschke, Thao Uyen Tran, Mohammad Naneh, Jutta Zagon, Matthias Winkel
Summary: In this study, a highly specific duplex PCR method was developed for the detection of most commercially relevant crustaceans in food. The limit of detection was 1 pg of crustacean DNA or 10 ppm of crustacean sample. Cross-testing with various plant and animal species ensured practical applicability, and a robustness test confirmed the practical use of the protocol.
