Journal
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Volume 39, Issue 4, Pages 589-607Publisher
SPRINGER
DOI: 10.1007/s00249-009-0547-5
Keywords
FRET; Lipid bilayer; Lipid raft; Membrane phase separation; Phase diagram
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Funding
- Fundacao para a Ciencia e Tecnologia (Portugal)
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Lateral membrane heterogeneity, in the form of lipid rafts and microdomains, is currently implicated in cell processes including signal transduction, endocytosis, and cholesterol trafficking. Various biophysical techniques have been used to detect and characterize lateral membrane domains. Among these, Forster resonance energy transfer (FRET) has the crucial advantage of being sensitive to domain sizes smaller than 50-100 nm, below the resolution of optical microscopy but, apparently, similar to those of rafts in cell membranes. In the last decade, several formalisms for the analysis of FRET in heterogeneous membrane systems have been derived and applied to the study of microdomains. They are critically described and illustrated here.
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