Article
Chemistry, Analytical
Meixing Li, Juan Cheng, Haitao Zheng, Jiawei Shi, Qingming Shen
Summary: A new electrochemical sensing strategy was developed for the precise detection of ultra-low abundance microRNA-21. The sensor utilized modified magnetic beads and hybridization chain reaction (HCR) to achieve signal amplification while avoiding modification fluctuation. The biosensor exhibited excellent specificity and reproducibility in complex sample analysis.
MICROCHEMICAL JOURNAL
(2022)
Article
Biophysics
Lingyi Kong, Shuzhen Lv, Zhenjie Qiao, Yongcun Yan, Jian Zhang, Sai Bi
Summary: In this study, a label-free electrochemical biosensing platform equipped with metal-organic frameworks (MOFs)-based nanoreactors has been developed for amplification detection of miRNA. The proposed biosensor exhibits excellent performance for miRNA-21 amplification detection, opening a new way for clinical analysis of miRNAs and early diagnosis of cancers.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Jairo Pinto de Oliveira, Francisco Burgos-Florez, Isabella Sampaio, Pedro Villalba, Valtencir Zucolotto
Summary: In this study, a label-free impedimetric immunosensor was developed to detect OTA in coffee. The biosensor showed high selectivity and was not affected by matrix interference in coffee samples. It has the advantages of small sample volume, short analysis time, and potential miniaturization.
Article
Chemistry, Analytical
Dongwei Wang, Jiasheng Wang
Summary: The study demonstrated the potential of functionalized polypyrrole nanowires as a platform for label-free miRNA detection using EIS, and constructed a sensitive and selective miRNA biosensor based on PPyNWs/PAMAM hybrid. The biosensor showed excellent sensing performances in monitoring DNA/miRNA hybridization, indicating the microstructure of the PPy nanowires array greatly improved its performance.
Article
Biochemistry & Molecular Biology
Li Li, Zhengbo Chen
Summary: In this study, a high specificity, flexibility, and simplicity aptasensor strategy was designed using a triblock DNA probe. It was found that the polyA fragment could assemble on the gold electrode surface via polyA instead of traditional Au-S bonds, leading to improved hybridization stability. The electrochemical aptasensor showed good repeatability, stability, and specificity.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Shuzhou Guo, Yaping Lin, Ziqi Lian, Tao Zeng, Liangliang Wang, Rongkai Ye, Benlin Hong, Qianwei Liang, Qianqing Wu, Jianqiang Hu, Aiqing Li
Summary: In this study, a label-free ultrasensitive biosensor was successfully developed for instant detection of miRNA-21, which has wide applications in early diagnosis of diseases like cancer and acute kidney injury. The biosensor showed high sensitivity, wide linear range, low limit of detection, and demonstrated specificity, stability, and reproducibility in detecting miRNA-21 in real urine samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Liang Zhu, Xiaolong Zhang, Yuanyuan Chang, Sai Xu, Ruo Yuan, Yaqin Chai
Summary: A novel co-catalytic nanocomposite Fc/HGQs/Fe3O4 was synthesized to significantly amplify the electrochemical signal of ferrocene, enabling ultrasensitive detection of miRNA-155. This approach opens a new avenue for exploring efficient signal labels for signal amplification and developing biosensors for biological detection and early clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Fangming Chen, Qian Guo, Qiaoyu Yang, Yao Meng, Mingrui Jiang, Jian Wang, De-Wen Zhang
Summary: Label-free electrochemical visualization of cancer cell apoptosis is achieved using a light-addressable electrochemical sensor. The study demonstrates that the AC photocurrent on apoptotic MCF-7 cells increases, indicating heterogeneity in apoptosis degrees. This new method provides potential applications in apoptosis-associated drug screening and efficacy evaluation.
ANALYTICAL CHEMISTRY
(2023)
Article
Polymer Science
Unchalee Kongintr, Benchaporn Lertanantawong, Chamras Promptmas
Summary: In this study, a label-free electrochemical biosensor for reliable detection of serum homocysteine (Hcy) was developed using a molecularly imprinted polymer (MIP) and nanocomposite. The Hcy-MIP biosensor showed high sensitivity, with a linear response range of 5.0 to 150 μM and a limit of detection of 1.2 μM. It demonstrated low cross-reactivity with other compounds and achieved good recoveries and repeatability. This novel biosensor offers a new and effective method for Hcy assay compared to existing techniques.
Article
Chemistry, Analytical
M. R. Ali, M. S. Bacchu, S. Das, S. Akter, M. M. Rahman, M. Aly Saad Aly, M. Z. H. Khan
Summary: A label-free electrochemical DNA biosensor for the selective detection of the foodborne bacterial pathogen Shigella flexneri has been developed. The biosensor exhibits a wide dynamic range, high specificity, and excellent recovery in detecting S. flexneri in food samples.
Article
Chemistry, Analytical
Yao Chen, Yu Zhong, Ji-Xing Ye, Yun Lei, Ai-Lin Liu
Summary: In this study, a sensitive and selective electrochemical DNA biosensor was developed for the determination of a osteosarcoma-related survivin gene. The proposed strategy used a simple and low-cost working electrode, with no need for other substances for electrode or DNA functionalization. The mode of interaction between celestine blue (CB) and DNA was studied, and it was found that the possible mode was intercalation with a binding number of 2 and a binding constant beta of 10(12.87). The label-free electrochemical DNA biosensor showed a good linear relationship towards the target gene and had a low detection limit of 0.046 nM.
Article
Chemistry, Analytical
Funa Meng, Zhongxi Jiang, Yajing Li, Peina Zhang, Haisheng Liu, Yanli Sun, Xueliang Wang
Summary: A enzyme-free and label-free electrochemical sensor was developed for detecting PNK activity. The sensor utilized the catalytic characteristic of PNK on DNA phosphorylation to achieve detection. The method showed good linear correlation and high sensitivity, making it a potential tool for PNK concentration detection in biological research and clinical diagnosis.
Article
Biophysics
Jose M. R. Flauzino, Emily P. Nguyen, Qiuyue Yang, Giulio Rosati, David Panacek, Ana G. Brito-Madurro, Joao M. Madurro, Aristides Bakandritsos, Michal Otyepka, Arben Merkoci
Summary: This study presents a label-free impedimetric genosensor for the sensitive detection of pork residues in meat, utilizing the biosensing capabilities of graphene acid. The sensor can detect pork residues in beef in less than 45 minutes, with a detection limit of 9%.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yinyin Liu, Ruhan Qiu, Zhicheng Zhang, Dawei Chen, Yushi Gao, Zongping Liu, Haidong Li, Chengyin Wang
Summary: In this study, a new electrochemical biosensor for Pb2+ detection was designed based on the interactions between nucleic acids and nanomaterials. The biosensor showed easy preparation, adsorptive tunability, and high sensitivity, making it suitable for potential applications in food safety inspection and environmental monitoring.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Kai Shi, Lei Cao, Fang Liu, Shiyi Xie, Shuo Wang, Yan Huang, Chunyang Lei, Zhou Nie
Summary: The study proposes an electrochemical biosensor for label-free and ultrasensitive detection of target protease biomarkers, utilizing PR signal amplification method, capable of sensitive detection of MMP-2 in a wide linear dynamic range and evaluation of MMP-2 activities in different cell cultures and human tissue samples, demonstrating the potential for analyzing protease biomarkers in complex clinical samples.
BIOSENSORS & BIOELECTRONICS
(2021)
