Article
Biotechnology & Applied Microbiology
Anahita Shariat, Fatemeh Sefidkon
Summary: The successful induction of tetraploidy in Satureja khuzitanica led to an increase in morphological parameters, biomass, essential oil yield, and phenolic compounds, indicating the potential for increased secondary metabolites and drug effectiveness in the plant.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biochemistry & Molecular Biology
Xinyang Li, Xueqian Zhang, Wenna Yang, Lanping Guo, Luqi Huang, Xia Li, Wenyuan Gao
Summary: This study compared the structural and physicochemical properties of native Banxia starch and autoclaving-cooling treated Banxia starch, finding that the CTBXS showed better thermal stability and higher SDS content. Both types of starch were able to change the composition of gut microbiota and promote the production of SCFAs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Sakineh Jokari, Akhtar Shekafandeh, Abolfazl Jowkar
Summary: In this study, tetraploids were induced in two Citrus species using colchicine, resulting in plants with superior morphological traits and enhanced physiological characteristics. These tetraploid plants have the potential to be used as rootstocks and as parents for future crossing.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Horticulture
Fazilat Fakhrzad, Abolfazl Jowkar, Akhtar Shekafandeh, Maryam Jafarkhani Kermani, Ali Moghadam
Summary: This study investigated the effects of polyploidy induction on different characteristics of wallflower. The results showed that chromosome doubled plants exhibited changes in trichome number, pollen size, stomata size, and chloroplast number in stomata guard cells. The tetraploid induced wallflowers also showed improvements in growth habit, leaf size and color, flowering period, flower longevity, and anthocyanin and total soluble solids contents. UPLC-MS analysis revealed changes in phytohormone contents in the tetraploid induced plants. These findings demonstrate that polyploidization is a promising tool for breeding and introducing new genetic resources in the floriculture industry.
SCIENTIA HORTICULTURAE
(2023)
Article
Agriculture, Multidisciplinary
H. Razmi, R. Amiri Fahliani, B. Kavoosi, A. Masoumiasl
Summary: This study induced polyploidy in strawberries using colchicine and found that a concentration of 0.7% was the most effective. The tetraploid plants showed larger fruit size and lower stomata number compared to the diploid plants.
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
(2022)
Article
Horticulture
Nasrin Sabooni, Ali Gharaghani, Abolfazl Jowkar, Saeid Eshghi
Summary: This research successfully generated polyploid blackberry plants using in vitro method, and found that different species reacted differently to polyploidy induction. Morphometric analysis of leaves showed that polyploidy affected guard cells, cell size, and cell count. This in vitro procedure could be a promising tool for creating blackberry cultivars with enhanced fruit characteristics and possible resistance to environmental stress.
SCIENTIA HORTICULTURAE
(2022)
Article
Agriculture, Multidisciplinary
Zhao Na, Xu Qing Guo, Wang Xu Jun
Summary: The study investigated the effect of drought stress on yield, physiological and biochemical indexes, and ecological parameters of artificially cultivated Pinellia ternata. The results showed that drought stress reduced leaf area, chlorophyll content, and soluble protein content, while increasing antioxidant enzyme activity. Different regions exhibited interspecies differences in these aspects.
PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES
(2022)
Article
Chemistry, Organic
Ming-Ling Wang, Rong Chen, Fu-Juan Sun, Peng-Ran Cao, Xing-Ru Chen, Ming-Hua Yang
Summary: Three new indole diketopiperazine alkaloids, aspergillines A-C (1-3), and one new polyketide, aspergillone A (4), were isolated from the solid cultures of an endophytic fungus Aspergillus cristatus. Aspergilline A is the first bicyclo[2.2.2] diazaoctane indole alkaloid constructed from tryptophan and alanine. Aspergillone A exhibited moderate antibacterial activities against Bacillus subtilis and Staphylococcus aureus.
TETRAHEDRON LETTERS
(2021)
Article
Plant Sciences
Liang Gu, Fu-Juan Sun, Cui-Ping Li, Le-Tian Cui, Ming-Hua Yang, Ling-Yi Kong
Summary: A new alkaloid and a dimeric derivative of citrinin were isolated from the solid culture of an endophytic Aspergillus terreus residing in Pinellia ternata. Penicitrinol Q showed inhibitory effects against Staphylococcus aureus and Bacillus subtilis, while compound 2 exhibited enhanced susceptibility to doxorubicin in treating doxorubicin-resistant human breast cancer cells.
PHYTOCHEMISTRY LETTERS
(2021)
Article
Plant Sciences
Juneyd Alavi, Asad Maroufi, Ghader Mirzaghaderi
Summary: The study demonstrates that induction of tetraploid plants in fenugreek through embryo culture treated with trifluralin is a more efficient method compared with colchicine, as trifluralin treatment results in higher survival rates of embryos and potentially increases secondary metabolite content. The elevated transcript expression of key genes involved in diosgenin biosynthesis in tetraploid plants suggests that trifluralin-induced tetraploidization may be beneficial for improving yield and quality of secondary metabolites in fenugreek.
ACTA PHYSIOLOGIAE PLANTARUM
(2022)
Article
Plant Sciences
Huihui Wang, Jitao Hu, Linying Li, Xueying Zhang, Hao Zhang, Zongsuo Liang, Qing Sheng, Yuqing He, Gaojie Hong
Summary: The study reveals that Pi starvation induces alkaloid and benzoic acid accumulation in Pinellia ternata. PtPHR1 plays a similar role to AtPHR1 in Pi signaling and metabolic responses.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Lifen Meng, Zhi Xia, Jingsong Lv, Gaofu Liu, Ye Tan, Qianzhu Li
Summary: This paper determined the strong allelopathy of five phenolic acids in the rhizosphere soil of Pinellia ternate to Pinellia ternate. The content changes of these phenolic acids were analyzed by comparing their contents in the rhizosphere soil before, during and after planting. The results showed that the five phenolic acids have inhib-itory effects on the growth of Pinellia ternate.
JOURNAL OF RADIATION RESEARCH AND APPLIED SCIENCES
(2022)
Article
Plant Sciences
Joe Abdul Vilcherrez-Atoche, Jessica Coutinho Silva, Wellington Ronildo Clarindo, Mateus Mondin, Jean Carlos Cardoso
Summary: This study aimed to develop a protocol for polyploidizing Blc. orchids by testing different explants (seeds and protocorms), concentrations, and exposure times to colchicine. The use of protocorms treated with colchicine resulted in the highest percentage of polyploid plants (16.4%), with the best outcome observed at a colchicine concentration of 500-750 mu M for 18 hours. Natural polyploidization in vitro using protocorms was also reported (11.5%). Cytological analysis allowed for the estimation of chromosome numbers in the parents, polyploidized progeny, and non-polyploidized progeny.
Article
Biotechnology & Applied Microbiology
Ruchira Bajpai, Rakhi Chaturvedi
Summary: In this study, in vitro doubled haploid (DH) plants were established for two tea cultivars (TV21 and TV19). Haploid embryos at the androgenic globular stage were diploidized using colchicine treatment and then multiplied through axillary-bud proliferation. The homozygosity of the diploidized plants was confirmed using SSR markers.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ya Cao, Xiaomeng Yu, Bing Han, Langjian Dong, Jingjing Xu, Yuhao Dai, Genxi Li, Jing Zhao
Summary: The electrochemical phenotyping method provides a low-cost and robust way to characterize stemlike phenotype in breast cancer, offering sensitivity, accuracy, and reliability. It can monitor phenotypic transition of cancer cells, drug effects, and determine stemlike phenotype, potentially supporting precise management of breast tumors.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
