Journal
EUPHYTICA
Volume 175, Issue 3, Pages 293-301Publisher
SPRINGER
DOI: 10.1007/s10681-010-0127-x
Keywords
Chlorophyll fluorescence; Cold acclimation; De-acclimation; Freezing tolerance; Molecular markers; Photosynthetic acclimation to cold
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Funding
- Polish Ministry of Science and Higher Education [2 P06A 002 28]
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Associations of PCR markers with freezing tolerance and acclimation of photosynthetic apparatus to cold were tested on 28 winter barley cultivars and advanced breeding forms to select alleles for practical application in marker assisted selection (MAS). We found significant associations between freezing tolerance evaluated with field-laboratory method (FLM) and markers located on 5H chromosome in region of gene Fr-H2 (bin9-10: Xbmag812, Xmwg2230) and region of gene Fr-H1 (bin11: Xmwg514, HvBM5, Xmwg644). Additionally, significant associations with photochemical quenching of chlorophyll a fluorescence (q(P)) were found for PCR markers Xpsr115 and Xmwg2062. In our study variation in the promoter region of Vrn-H1 (HvBM5) was directly connected with freezing tolerance of plants partially de-acclimated in the field. The results obtained here showed that different loci of freezing tolerance may play role in variable selection pressure of winter conditions.
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