Article
Plant Sciences
Shun Tamaki, Kazunari Ozasa, Toshihisa Nomura, Marumi Ishikawa, Koji Yamada, Kengo Suzuki, Keiichi Mochida
Summary: Zeaxanthin is required for the formation of functional photoreactive organelles in Euglena gracilis, highlighting the evolutionary differences between euglenophytes and chlorophytes in the photosensing mechanism. The study demonstrates the metabolic regulation of photoreactive organelle formation in Euglena gracilis and its importance in eyespot formation.
Article
Biotechnology & Applied Microbiology
Fan Bai, Yu Zhang, Jin Liu
Summary: bZIP transcription factors play important roles in lipid and pigment metabolisms in microalgae, as shown by the comparative analysis of crbzip2 mutant and wild-type strain in Chlamydomonas reinhardtii. The disruption of CrBZIP2 leads to reduced TAG accumulation, increased DGDG production, and up-regulated biosynthetic pathways of carotenoids and chlorophylls. These findings suggest that CrBZIP2 could be a potential target for genetic engineering to manipulate lipid and pigment production in microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
E. Hounslow, C. A. Evans, J. Pandhal, T. Sydney, N. Couto, T. K. Pham, D. James Gilmour, P. C. Wright
Summary: Physiological and proteomic changes between two green alga strains, C. reinhardtii and C. nivalis, under salinity stress were characterized. Results showed that C. nivalis accumulated more carbohydrates and fatty acids under salt stress compared to C. reinhardtii. The differences in protein abundance provided insights into the mechanism by which salt stress promotes fatty acid accumulation in C. nivalis.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Qiulan Luo, Xianghui Zou, Chaogang Wang, Yajun Li, Zhangli Hu
Summary: The study revealed the important roles of three cullins (CrCULs) in lipid metabolism in Chlamydomonas reinhardtii. Among them, CrCUL2 and CrCUL4 were identified as regulators for lipid accumulation. These findings provide valuable insights into lipid biosynthesis in microalgae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Emma O. Fuentes-Ramirez, Miriam Vazquez-Acevedo, Alfredo Cabrera-Orefice, Sergio Guerrero-Castillo, Diego Gonzalez-Halphen
Summary: This study provides a morphological and proteomic characterization of the colorless alga Polytomella parva, identifying several plastid proteins involved in various metabolic pathways. The results contribute to a better understanding of the metabolism and biological activities of this unicellular, free-living alga.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sibaji K. Sanyal, Komal Sharma, Diksha Bisht, Sunita Sharma, Kumari Sushmita, Suneel Kateriya, Girdhar K. Pandey
Summary: Ca2+ signaling is crucial for perceiving and transmitting information in cells. The Ca2+-mediated CBL-CIPK module is a major sensor responder network for Ca2+ signaling, and its involvement in plant adaptation from aquatic to terrestrial habitats has been suggested. Experimental analysis showed that CrCBL1 protein interacts with Phototropin and Channelrhodopsin, and its expression is regulated by light. Comparing algal sequences, differences in CBL and CIPK proteins between aquatic and terrestrial plants were identified. Additionally, Klebsormidium CBL and CIPK genes were found to respond to abiotic stress, indicating their early role in terrestrial adaptations. Structure-based prediction and Ca2+-binding assays revealed a typical Ca2+-binding pocket in Klebsormidium's KnCBL1 protein. Overall, this study suggests that stress-responsive proteins facilitate crosstalk between Ca2+ and light signaling pathways during plant adaptation from aquatic to terrestrial habitats.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Jiale Qiu, Jie Zhang, Huihui Zhao, Cuiping Wu, Caoliang Jin, Xiangdong Hu, Jian Li, Xiuling Cao, Shenkui Liu, Xuejiao Jin
Summary: Carbonate stress has significant impacts on agriculture and industry, but the role of cell wall-related genes in resistance to carbonate remains poorly understood. This study reveals the critical role of cellulose in the NaHCO3 resistance of Chlorella JB17, and identifies a NaHCO3-tolerance gene that could be used for crop breeding and genetic modification of microalgae for biofuel production.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Yutaro Oishi, Rie Otaki, Yukari Iijima, Eri Kumagai, Motohide Aoki, Mikio Tsuzuki, Shoko Fujiwara, Norihiro Sato
Summary: Oishi et al. discovered that a green alga Chlorella kessleri synthesized a betaine lipid DGTS to replace phosphatidylcholine and phosphatidylethanolamine during phosphate deprivation. This study sheds light on the phosphorus-deficiency acclimation strategies in lower green plants through lipid remodeling.
COMMUNICATIONS BIOLOGY
(2022)
Article
Microbiology
Vanessa Brisson, Xavier Mayali, Benjamin Bowen, Amber Golini, Michael Thelen, Rhona K. Stuart, Trent R. Northen
Summary: This study analyzed nonpolar exometabolites released by diverse eukaryotic microalgal strains and identified unique metabolites that could impact algal growth when provided exogenously. The findings highlight the importance of characterizing novel microalgal exometabolites for understanding algal interactions and potential roles in allelopathy.
Article
Plant Sciences
Solene L. Y. Moulin, Audrey Beyly-Adriano, Stephan Cuine, Stephanie Blangy, Bertrand Legeret, Magali Floriani, Adrien Burlacot, Damien Sorigue, Poutoum-Palakiyem Samire, Yonghua Li-Beisson, Gilles Peltier, Fred Beisson
Summary: Fatty acid photodecarboxylase (FAP) is a photoenzyme that requires light for catalytic cycle, and recent studies have shown its conservation and role in the formation of hydrocarbons in algae such as Chlamydomonas reinhardtii. This enzyme is predominantly membrane-associated and its activity is linked to photosynthetic membranes, suggesting a potentially important role in the evolution and function of algal lineages.
Article
Plant Sciences
Yi-Ying Lee, Rudolph Park, Stephen M. Miller, Yantao Li
Summary: Genetic compensation is proposed as a mechanism to explain phenotypic differences between gene knockouts and knockdowns. This study demonstrates examples of genetic compensation in microalgae and suggests its physiological relevance in TAG biosynthesis under stress. These findings provide guidance for future genetic engineering and mutant characterization efforts.
Article
Multidisciplinary Sciences
Amnah Alzahmi, Sarah Daakour, Diana Charles El Assal, Bushra S. Dohai, Amphun Chaiboonchoe, Weiqi Fu, David R. Nelson, Alexandra Mystikou, Kourosh Salehi-Ashtiani
Summary: Metabolic models are reconstructed based on available genome annotation to study metabolic processes systematically, with gaps and unverified reactions. Phenotype microarray technology, combined with metabolic modeling, can rapidly expand and refine metabolic network models using experimental evidence. In this study, PM assays were used to expand and refine a genome-scale C. reinhardtii metabolic network model, demonstrating its potential for studying microalgae.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Electrochemistry
Adnan Sayegh, Luca A. Perego, Marc Arderiu Romero, Louis Escudero, Jerome Delacotte, Manon Guille-Collignon, Laurence Grimaud, Benjamin Bailleul, Frederic Lemaitre
Summary: This study focuses on extracting energy from oxygenic photosynthesis by rerouting electron flow using intact photosynthetic organisms to generate photocurrent. Researchers found that some quinones can interact with the embedded photosynthetic chain, but the balance between toxicity and bioelectricity production needs to be considered.
Article
Biochemistry & Molecular Biology
Amina Antonacci, Valeria Frisulli, Lucas Braganca Carvalho, Leonardo Fernandes Fraceto, Bruno Miranda, Luca De Stefano, Udo Johanningmeier, Maria Teresa Giardi, Viviana Scognamiglio
Summary: A completely green biosensor was designed using eco-friendly materials to detect herbicides encapsulated in biodegradable nanoparticles for sustainable agriculture. The nanocarriers delivered herbicides to the correct location, reducing their impact on plants and the agricultural and food industries. Comprehensive information about nanoformulated herbicides in agricultural fields is crucial to support farmers in decision-making.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Mohammad Yazdani, Michelle G. Croen, Tara L. Fish, Theodore W. Thannhauser, Beth A. Ahner
Summary: The overexpression of wild type CrORWT and mutated CrORHis in microalgae significantly increased the accumulation of multiple carotenoids and upregulated the transcription levels of related biosynthetic genes, leading to enhanced tolerance to salt and paraquat stress. Additionally, the endogenous phytohormone abscisic acid (ABA) levels were also elevated in the transgenic cell lines under both normal growth conditions and stress conditions, suggesting a potential role of OR in modulating oxidative stress tolerance.
METABOLIC ENGINEERING
(2021)