Article
Biochemistry & Molecular Biology
Vendula Horackova, Lubos Voleman, Kari D. Hagen, Marketa Petru, Martina Vinopalova, Filip Weisz, Natalia Janowicz, Lenka Markova, Alzbeta Motyckova, Vladimira Najdrova, Pavla Tumova, Scott C. Dawson, Pavel Dolezal
Summary: This study demonstrated successful genome editing of G. intestinalis using CRISPR/Cas9 technology, achieving effective gene knockout and providing important tools for further research on parasitic protists.
Article
Cell Biology
Saravanan Subramaniam, Jiandong Liu, Craig Fletcher, Ramani Ramchandran, Hartmut Weiler
Summary: In this study, the role of f3a in embryonic development was characterized using a zebrafish model. The findings revealed that f3a is crucial for yolk sac development, circulation, vascular patterning, and angiogenesis in addition to its involvement in hemostasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Zhongyu Wang, Xinli Fan, Guanqun Mu, Xiaoran Zhao, Qian Wang, Jing Wang, Xinjing Tang
Summary: This study developed CB-activatable cASOs and successfully inhibited disease-related genes in tumor cells with limited effects on normal cells. Compared to linear cASOs, CB-activatable cASOs also reduced nonspecific immunostimulation.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Review
Immunology
Suthinee Sangkanu, Alok K. K. Paul, Julalak Chuprom, Watcharapong Mitsuwan, Rachasak Boonhok, Maria de Lourdes Pereira, Sonia Marlene Rodrigues Oliveira, Polrat Wilairatana, Mohammed Rahmatullah, Christophe Wiart, Muhammad Nawaz, Chea Sin, Sunil Kayesth, Veeranoot Nissapatorn
Summary: Giardia infection can cause diarrhea and is the third most common cause of diarrhea and death in children under five. Antimicrobial drugs are commonly used to treat giardiasis, but vaccination may be a potential means of control and protection against this parasitic disease outbreak.
Article
Parasitology
Min Sun, Zhiteng Zhao, Ying Li, Lili Cao, Jianhua Li, Xichen Zhang, Xin Li, Nan Zhang, Shuqin Cheng, Xiaocen Wang, Pengtao Gong
Summary: Giardia intestinalis parasite evades host immune responses through surface proteins called VSPs. This study found that VSPAS7 protein in Giardia inhibits activation of signaling pathways and pyroptosis in host macrophages, allowing Giardia to evade host immune responses.
PARASITES & VECTORS
(2023)
Article
Public, Environmental & Occupational Health
Tanja E. Peters, Benno Kreuels, Marylyn M. Addo, Egbert Tannich, Camilla Rothe
Summary: This study analyzed 339 cases of returning travellers with confirmed giardiasis between 2007 and 2016 in Hamburg, Germany, finding a significantly higher risk of treatment failure with metronidazole for patients returning from South Asia. Second-line treatment regimens showed inconsistency and unsatisfactory results, suggesting a need for reevaluation of treatment strategies for giardiasis. Prospective trials are urgently needed to assess new first-line treatment options and improve second-line treatment strategies.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2021)
Article
Ophthalmology
Marina Rodriguez-Arrizabalaga, Ismael Hernandez-Nunez, Eva Candal, Anton Barreiro-Iglesias
Summary: Work in the catshark Scyliorhinus canicula reveals that postnatal neurogenesis in vertebrates originated earlier than previously assumed. This makes the catshark an interesting model for studying postnatal neurogenic processes and their evolution in vertebrates. However, the lack of genetic tools in sharks inhibits in-depth research on the genes involved in neurogenesis. In this study, the researchers developed a method using Vivo-Morpholinos to knock down genes in the catshark retina, specifically targeting the proliferation marker PCNA. The method was found to effectively reduce mitotic activity in the peripheral retina, providing a valuable tool for studying the role of other genes in postnatal neurogenesis in this animal model.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Developmental Biology
Anirban Bardhan, Alexander Deiters, Charles A. Ettensohn
Summary: Echinoderms are important experimental models for analyzing embryonic development, with morpholino antisense oligonucleotides (MOs) being widely used for gene knockdowns. The external development and optical transparency of echinoderm embryos make them suitable for light-based approaches. The use of caged MOs synthesized using nucleobase-caged monomers provides light-regulated control over gene expression in sea urchin embryos.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Zhiqiang Li, Dimitra Peirasmaki, Staffan Svard, Magnus Abrink
Summary: The proteoglycan serglycin plays a regulatory role in intestinal immune responses by influencing the expression levels of chemokines and cytokines, leading to delayed weight gain in young SG(-/-) mice infected with Giardia intestinalis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Malgorzata Smogula, Roland Wesolowski, Marta Pawlowska, Celestyna Mila-Kierzenkowska
Summary: Giardia intestinalis is a common food-borne protozoan, and 22 selected factors were found to have an impact on its survival assessment and detection. Microscopic method showed significant influence compared to real-time PCR. Prevention of infection is crucial, and further research and epidemiological observation are needed.
Article
Multidisciplinary Sciences
Ying Zhang, Xuemei Xie, Pourya Naderi Yeganeh, Dian-Jang Lee, David Valle-Garcia, Karla F. Meza-Sosa, Caroline Junqueira, Jiayu Su, Hongbo R. Luo, Winston Hide, Judy Lieberman
Summary: New cancer immunotherapy strategies using EpCAM aptamer-linked small-interfering RNA chimeras (AsiCs) were effective in selectively knocking down genes in EpCAM(+) tumors to enhance immune recognition. Four of the six AsiC (Upf2, Parp1, Cd47, and Mcl1) were able to inhibit tumor growth and improve tumor-infiltrating immune cell functions. AsiC mixtures showed better efficacy than individual AsiC and could synergize with anti-PD-1 checkpoint inhibition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Wes Brown, Anirban Bardhan, Kristie Darrah, Michael Tsang, Alexander Deiters
Summary: The study reveals that light-activated circular morpholino oligonucleotides can be used to conditionally control microRNA function and study their role in zebrafish embryo development. Precise spatial and temporal control over microRNA function was achieved through irradiation, providing insights into the cell populations and developmental timepoints involved in each process.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Medicine, General & Internal
Aleksandra Lanocha, Natalia Lanocha-Arendarczyk, Dominika Wilczynska, Barbara Zdziarska, Danuta Kosik-Bogacka
Summary: This study aimed to evaluate the frequency of gastrointestinal protozoan infection in patients with hematological malignancies undergoing intensive treatment and to determine the biological factors influencing intestinal parasite infection. The results showed a higher prevalence of parasitic infections in patients with hematological malignancies undergoing treatment.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Kristyna Brozova, Milan Jirku, Zuzana Lhotska, Dana Kvetonova, Oldriska Kadlecova, Christen Rune Stensvold, Peter Samas, Klara J. Petrzelkova, Katerina Jirku
Summary: This study investigated the presence and epidemiology of Giardia in healthy volunteers in the Czech Republic and compared different diagnostic methods. The results showed that qPCR is the most sensitive method for detecting Giardia, and the prevalence of infection in asymptomatic individuals was 7%. Contact with animals was found to be the only factor significantly impacting the incidence of Giardia in healthy humans.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Biology
Abel Sana, Izadora Volpato Rossi, Bruna Sabatke, Leticia Bassani Bonato, Lia Carolina Soares Medeiros, Marcel Ivan Ramirez
Summary: This study aimed to enrich the large extracellular vesicles (LEVs) of G. intestinalis and better understand their role in the parasite's interaction with the host. The researchers modified their method and compared different centrifugation techniques to concentrate the EVs. They found that direct ultracentrifugation and longer centrifugation time were more effective in enriching the EVs.
Article
Multidisciplinary Sciences
Hannes Schroeder, Maria C. Avila-Arcos, Anna-Sapfo Malaspinas, G. David Poznik, Marcela Sandoval-Velasco, Meredith L. Carpenter, Jose Victor Moreno-Mayar, Martin Sikora, Philip L. F. Johnson, Morten Erik Allentoft, Jose Alfredo Samaniego, Jay B. Haviser, Michael W. Dee, Thomas W. Stafford, Antonio Salas, Ludovic Orlando, Eske Willerslev, Carlos D. Bustamante, M. Thomas P. Gilbert
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Ecology
Maria C. Avila-Arcos, Marcela Sandoval-Velasco, Hannes Schroeder, Meredith L. Carpenter, Anna-Sapfo Malaspinas, Nathan Wales, Fernando Penaloza, Carlos D. Bustamante, M. Thomas P. Gilbert
METHODS IN ECOLOGY AND EVOLUTION
(2015)
Article
Multidisciplinary Sciences
Constanza de la Fuente, Mara C. Avila-Arcos, Jacqueline Galimany, Meredith L. Carpenter, Julian R. Homburger, Alejandro Blanco, Paloma Contreras, Diana Cruz Davalos, Omar Reyes, Manuel San Roman, Andres Moreno-Estrada, Paula F. Campos, Celeste Eng, Scott Huntsman, Esteban G. Burchard, Anna-Sapfo Malaspinas, Carlos D. Bustamante, Eske Willerslev, Elena Llop, Ricardo A. Verdugo, Mauricio Moraga
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Genetics & Heredity
Meredith L. Carpenter, Jason D. Buenrostro, Cristina Valdiosera, Hannes Schroeder, Morten E. Allentoft, Martin Sikora, Morten Rasmussen, Simon Gravel, Sonia Guillen, Georgi Nekhrizov, Krasimir Leshtakov, Diana Dimitrova, Nikola Theodossiev, Davide Pettener, Donata Luiselli, Karla Sandoval, Andres Moreno-Estrada, Yingrui Li, Jun Wang, M. Thomas P. Gilbert, Eske Willerslev, William J. Greenleaf, Carlos D. Bustamante
AMERICAN JOURNAL OF HUMAN GENETICS
(2013)
Article
Biotechnology & Applied Microbiology
Jeffrey M. Kidd, Thomas J. Sharpton, Dean Bobo, Paul J. Norman, Alicia R. Martin, Meredith L. Carpenter, Martin Sikora, Christopher R. Gignoux, Neda Nemat-Gorgani, Alexandra Adams, Moraima Guadalupe, Xiaosen Guo, Qiang Feng, Yingrui Li, Xiao Liu, Peter Parham, Eileen G. Hoal, Marcus W. Feldman, Katherine S. Pollard, Jeffrey D. Wall, Carlos D. Bustamante, Brenna M. Henn
Article
Biochemistry & Molecular Biology
Lillian K. Fritz-Laylin, Simon E. Prochnik, Michael L. Ginger, Joel B. Dacks, Meredith L. Carpenter, Mark C. Field, Alan Kuo, Alex Paredez, Jarrod Chapman, Jonathan Pham, Shengqiang Shu, Rochak Neupane, Michael Cipriano, Joel Mancuso, Hank Tu, Asaf Salamov, Erika Lindquist, Harris Shapiro, Susan Lucas, Igor V. Grigoriev, W. Zacheus Cande, Chandler Fulton, Daniel S. Rokhsar, Scott C. Dawson
Article
Cell Biology
Meredith L. Carpenter, Zoe June Assaf, Stephane Gourguechon, W. Zacheus Cande
JOURNAL OF CELL SCIENCE
(2012)
Article
Multidisciplinary Sciences
Mansi Srivastava, Emina Begovic, Jarrod Chapman, Nicholas H. Putnam, Uffe Hellsten, Takeshi Kawashima, Alan Kuo, Therese Mitros, Asaf Salamov, Meredith L. Carpenter, Ana Y. Signorovitch, Maria A. Moreno, Kai Kamm, Jane Grimwood, Jeremy Schmutz, Harris Shapiro, Igor V. Grigoriev, Leo W. Buss, Bernd Schierwater, Stephen L. Dellaporta, Daniel S. Rokhsar
Article
Multidisciplinary Sciences
Marianne K. Poxleitner, Meredith L. Carpenter, Joel J. Mancuso, Chung-Ju R. Wang, Scott C. Dawson, W. Zacheus Cande
Article
Genetics & Heredity
Martin Sikora, Meredith L. Carpenter, Andres Moreno-Estrada, Brenna M. Henn, Peter A. Underhill, Federico Sanchez-Quinto, Ilenia Zara, Maristella Pitzalis, Carlo Sidore, Fabio Busonero, Andrea Maschio, Andrea Angius, Chris Jones, Javier Mendoza-Revilla, Georgi Nekhrizov, Diana Dimitrova, Nikola Theodossiev, Timothy T. Harkins, Andreas Keller, Frank Maixner, Albert Zink, Goncalo Abecasis, Serena Sanna, Francesco Cucca, Carlos D. Bustamante
Article
Agriculture, Dairy & Animal Science
Danielle Bisbee, Meredith L. Carpenter, Katie Hoefs-Martin, Samantha A. Brooks, Christa Lafayette
Article
Infectious Diseases
Divya Shah, Julianne R. Brown, Jack C. D. Lee, Meredith L. Carpenter, Gavin Wall, Judith Breuer
Summary: This study evaluated the performance of the Galileo Viral Panel in detecting and quantifying DNA viruses compared to standard qPCR assays. The results showed high agreement between Galileo and qPCR in terms of qualitative and quantitative detection of viruses. Galileo also identified additional viruses that were not tested with routine qPCR, which could have an impact on clinical outcomes.
JOURNAL OF CLINICAL VIROLOGY PLUS
(2022)
Article
Agriculture, Dairy & Animal Science
H. M. Holl, J. Vanhnasy, R. E. Everts, K. Hoefs-Martin, D. Cook, S. A. Brooks, M. L. Carpenter, C. D. Bustamante, C. Lafayette
