Article
Biochemistry & Molecular Biology
Colin G. Miller, Jean A. Kundert, Justin R. Prigge, Julie A. Amato, Allison E. Perez, Lucia Coppo, Gabrielle N. Rizzo, Michael P. Kavanaugh, David J. Orlicky, Colin T. Shearn, Edward E. Schmidt
Summary: While ascorbate supplementation did not protect against liver failure in TrxR1/Gsr-null livers, it unexpectedly increased the death rate. However, it was found that ascorbate treatment reduced basal DNA damage and oxidative stress markers despite depleting GSH in the stressed livers.
Article
Chemistry, Analytical
Fang Zhang, Fan Chen, Ruipeng Shen, Ya-xiong Chen, Zhengjia Zhao, Baoxin Zhang, Jianguo Fang
Summary: This study reports the construction of a probe library based on the naphthalimide skeleton to detect glutathione (GSH). Compound R13 was identified as a highly efficient GSH fluorescent probe and could be used to quantify GSH in cells and tissues. The probe was applied to study the alteration of GSH level in mouse livers after X-ray irradiation and Parkinson's mouse brains, providing valuable insights into the fluctuation of GSH/GSSG ratio in diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Kaili Anier, Kelli Somelar, Kulli Jaako, Margret Alttoa, Kerli Sikk, Raul Kokassaar, Kai Kisand, Anti Kalda
Summary: This study found that repeated exposure to psychostimulants decreases the enzymatic activity of TETs in PBMCs. Co-treatment with decitabine can restore TETs levels and modulate immune response after repeated cocaine exposure. These findings may contribute to further research on biomarkers and therapeutic approaches for psychostimulant use.
CLINICAL EPIGENETICS
(2022)
Article
Sport Sciences
Josh Thorley, Craig Thomas, Stephen J. Bailey, Neil R. W. Martin, Nicolette C. Bishop, Tom Clifford
Summary: This study examined the impact of eccentric exercise on NRF2 activity in PBMCs. The results showed that eccentric exercise increased NRF2/ARE binding in PBMCs compared to resting.
JOURNAL OF SPORTS SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mirva J. Saaranen, Heli Alanen, Kirsi E. H. Salo, Emmanuel Nji, Pekka Karkkainen, Constanze Schmotz, Lloyd W. Ruddock
Summary: Proteins in the thioredoxin superfamily, such as protein disulfide isomerase (PDI) and glutaredoxins, have similar structures and catalytic mechanisms. PDI uses glutathione for oxidation/reduction in vitro, while glutaredoxins have a high affinity for glutathione. Mutating the active site of PDI to a more glutaredoxin-like motif increases its reactivity with glutathione.
Article
Biochemistry & Molecular Biology
Federico Maria Rubino
Summary: Glutathionyl hemoglobin is a minor form of hemoglobin that plays a crucial role in the response of erythrocytes to oxidative stress. By measuring the redox potential of its active site, insights into the changes in erythrocytes during oxidative and recovery phases can be gained. Research indicates that hemoglobin acts as an important redox buffer in erythrocytes.
Article
Biochemistry & Molecular Biology
Shengchao Ma, Guanjun Lu, Qing Zhang, Ning Ding, Yuzhen Jie, Hui Zhang, Lingbo Xu, Lin Xie, Xiaoling Yang, Huiping Zhang, Yideng Jiang
Summary: In this study, we found that Hcy can increase the risk of oxidative stress and atherosclerosis by affecting the DNA methylation status of EC-SOD. The key enzyme in this process was identified as DNMT1.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Biochemistry & Molecular Biology
Debaprasad Koner, Niharika Nag, Parismita Kalita, Aditya K. Padhi, Timir Tripathi, Nirmalendu Saha
Summary: The presence of thioredoxin glutathione reductase (TGR) in the facultative air-breathing catfish, Clarias magur, was identified for the first time. The TGR enzyme showed both TrxR and GR activities, contributing to the maintenance of redox homeostasis and antioxidant defense under oxidative stress induced by environmental stressors. Phylogenetic analysis revealed its relationship to fish TrxRs and distant relation to platyhelminth parasites TGRs. The characterization of C. magur TGR provides insights into its unique adaptation to environmental challenges.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Dmitry S. Semenovich, Egor Yu Plotnikov, Oksana Titko, Elena P. Lukiyenko, Nina P. Kanunnikova
Summary: This study highlighted the importance of the glutathione system in brain mitochondria for maintaining redox balance and thiol-disulfide homeostasis, particularly in neurodegenerative diseases. Mitochondrial dysfunction can lead to changes in energy metabolism, free radical processes, and apoptotic signals. The research also showed that pantothenic acid derivatives like panthenol can significantly improve enzyme activity in mitochondrial energy metabolism, normalize the redox potential of the glutathione system, and reduce levels of S-glutathionylated proteins in brain mitochondria.
Article
Food Science & Technology
Melanie Hemgesberg, Simone Stegmueller, Alexander Cartus, Selina Hemmer, Michael Puettmann, Jean-Pierre Stockis, Dieter Schrenk
Summary: The study found that the presence of AA-derived DNA adducts in human PBMC is related to body weight rather than specific dietary or lifestyle factors.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Rheumatology
Ilka Liebold, Andreas Gruetzkau, Anika Goeckeritz, Velia Gerl, Randall Lindquist, Eugen Feist, Michael Zaenker, Thomas Haeupl, Denis Poddubnyy, Jan Zernicke, Biljana Smiljanovic, Tobias Alexander, Gerd R. Burmester, Steffen Gay, Bruno Stuhlmueller
Summary: The study investigated global DNA methylation in peripheral blood mononuclear cells of RA patients and found that it was associated with disease activity and treatment response. The degree of global methylation may be a promising biomarker for monitoring therapy and predicting outcomes in inflammatory diseases.
Article
Immunology
Anna Baranska, Agnieszka Wozniak, Katarzyna Mokra, Jaromir Michalowicz
Summary: Bromophenolic flame retardants (BFRs) are synthetic substances used to reduce flammability in various materials. This study compared the impact of different BFRs on DNA damage in human peripheral blood mononuclear cells (PBMCs). The results showed that the BFRs induced DNA strand-breaks and oxidative damage, which PBMCs were partially able to repair. The extent of damage varied among the different BFRs, with TBBPA causing the most significant changes. Additionally, the tested compounds did not form adducts with DNA, suggesting that the observed damage was likely caused by indirect DNA-damaging agents.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ying Zhao, Xin Zuo, Shuang Liu, Wenjun Qian, Xuewen Tang, Jun Lu
Summary: The Trx and Grx systems are critical disulfide reductase systems in cell activities, with significant differences between prokaryotes and mammals. Fast-TRFS can be utilized to detect cellular disulfide reducibility in bacteria, where Trx system is shown to be the predominant enzyme for fast disulfide reduction.
Review
Medicine, General & Internal
Francois Sauer, Marianne Riou, Anne-Laure Charles, Alain Meyer, Emmanuel Andres, Bernard Geny, Samy Talha
Summary: Heart failure is a leading cause of hospitalization in patients aged over 65 and is associated with high mortality rates. Recent studies have focused on the mitochondrial respiration of peripheral blood circulating cells (PBMCs) in order to better understand the physiopathology of HF. These studies suggest that cardiovascular metabolic risk factors and cellular switch may contribute to decreased PBMC mitochondrial respiration, which is associated with HF severity. Further research is needed to investigate the potential diagnostic, prognostic, and therapeutic utility of monitoring PBMC mitochondrial function and mitoDAMPs in HF patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ildete L. Ferreira, Solange Costa, Bruno J. Moraes, Ana Costa, Olga Fokt, Daniela Marinho, Vera Alves, Isabel P. Baptista, A. Cristina Rego
Summary: Periodontitis (PDT) and type 2 diabetes (T2D) have a bidirectional relationship and are associated with imbalanced oxidative stress and mitochondrial dysfunction. This study investigated the mitochondrial and redox (de)regulation in peripheral blood mononuclear cells (PBMCs) of T2D-PDT, PDT, T2D patients, and control individuals. The results showed that PBMCs from T2D-PDT patients exhibited significant redox changes, while PBMCs from PDT patients showed mitochondrial deregulation and reduced expression of Nrf2-target genes.
Editorial Material
Immunology
Kristin N. Harper
Article
Cardiac & Cardiovascular Systems
Shafiul Alam, Chowdhury S. Abdullah, Richa Aishwarya, Sumitra Miriyala, Manikandan Panchatcharam, Jonette M. Peretik, A. Wayne Orr, Jeanne James, Jeffrey Robbins, Md. Shenuarin Bhuiyan
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2018)
Article
Cardiac & Cardiovascular Systems
Chowdhury S. Abdullah, Shafiul Alam, Richa Aishwarya, Sumitra Miriyala, Manikandan Panchatcharam, Mohammad Alfrad Nobel Bhuiyan, Jonette M. Peretik, A. Wayne Orr, Jeanne James, Hanna Osinska, Jeffrey Robbins, John N. Lorenz, Md. Shenuarin Bhuiyan
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2018)
Editorial Material
Immunology
Kirsten Nicole Harper
Editorial Material
Immunology
Kirsten Nicole Harper
Editorial Material
Immunology
Kirsten Nicole Harper
Article
Multidisciplinary Sciences
Chowdhury S. Abdullah, Shafiul Alam, Richa Aishwarya, Sumitra Miriyala, Mohammad Alfrad Nobel Bhuiyan, Manikandan Panchatcharam, Christopher B. Pattillo, A. Wayne Orr, Junichi Sadoshima, Joseph A. Hill, Md. Shenuarin Bhuiyan
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Lenka Mikalova, Klara Janeckova, Marketa Novakova, Michal Strouhal, Darina Cejkova, Kristin N. Harper, David Smajs
Article
Biochemistry & Molecular Biology
Richa Aishwarya, Shafiul Alam, Chowdhury S. Abdullah, Mahboob Morshed, Sadia S. Nitu, Manikandan Panchatcharam, Sumitra Miriyala, Christopher G. Kevil, Md Shenuarin Bhuiyan
Article
Cardiac & Cardiovascular Systems
Shafiul Alam, Chowdhury S. Abdullah, Richa Aishwarya, Mahboob Morshed, Sadia S. Nitu, Sumitra Miriyala, Manikandan Panchatcharam, Christopher G. Kevil, A. Wayne Orr, Md Shenuarin Bhuiyan
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2020)
Article
Biology
Chowdhury S. Abdullah, Richa Aishwarya, Shafiul Alam, Mahboob Morshed, Naznin Sultana Remex, Sadia Nitu, Gopi K. Kolluru, James Traylor, Sumitra Miriyala, Manikandan Panchatcharam, Brandon Hartman, Judy King, Mohammad Alfrad Nobel Bhuiyan, Sunitha Chandran, Matthew D. Woolard, Xiuping Yu, Nicholas E. Goeders, Paari Dominic, Connie L. Arnold, Karen Stokes, Christopher G. Kevil, A. Wayne Orr, Md. Shenuarin Bhuiyan
COMMUNICATIONS BIOLOGY
(2020)
Article
Biology
Alison F. Feder, Kristin N. Harper, Chanson J. Brumme, Pleuni S. Pennings
Summary: Triple-drug therapies have transformed HIV from a fatal condition to a chronic one, significantly reducing the risk of drug resistance evolution, but not eliminating it entirely.
Article
Pathology
Richa Aishwarya, Chowdhury S. Abdullah, Naznin S. Remex, Shaful Alain, Mahboob Morshed, Sadia Nitu, Brandon Hartman, Judy King, Mohammad Alfrad Nobel Bhuiyan, A. Wayne Orr, Christopher G. Kevil, Shenuarin Bhuiyan
Summary: Sigma 1 receptor (Sigmar1) is a multifunctional molecular chaperone protein with mutations linked to skeletal muscle dysfunction. Studies on Sigmar1-/- mice revealed abnormalities in skeletal muscle structure and function, including altered myofiber size distribution, abnormal mitochondria, and muscle pathology.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Cell Biology
Chowdhury S. Abdullah, Richa Aishwarya, Shafiul Alam, Naznin Sultana Remex, Mahboob Morshed, Sadia Nitu, Sumitra Miriyala, Manikandan Panchatcharam, Brandon Hartman, Judy King, Mohammad Alfrad Nobel G. Bhuiyan, James Traylor, Christopher Kevil, A. Wayne Orr, Shenuarin Bhuiyan
Summary: This study reveals the localization of Sigmar1 on mitochondrial membranes in cardiomyocytes and its essential role in maintaining mitochondrial respiratory homeostasis.
Article
Linguistics
Jacob L. Mey
REVUE ROUMAINE DE LINGUISTIQUE-ROMANIAN REVIEW OF LINGUISTICS
(2018)