Article
Cell & Tissue Engineering
Yoichi Sekita, Yuki Sugiura, Akari Matsumoto, Yuki Kawasaki, Kazuya Akasaka, Ryo Konno, Momoka Shimizu, Toshiaki Ito, Eiji Sugiyama, Terushi Yamazaki, Eriko Kanai, Toshinobu Nakamura, Makoto Suematsu, Fumitoshi Ishino, Yoshio Kodera, Takashi Kohda, Tohru Kimura
Summary: The study reveals that AKT signaling is involved in TET-mediated DNA demethylation during cellular reprogramming, enhancing glucose metabolism and increasing levels of alpha-ketoglutarate. This leads to genome-wide changes in DNA methylation and upregulation of pluripotency genes. AKT activation promotes epigenetic reprogramming by hyperactivating TET at transcriptional and catalytic levels.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Carlos A. M. Cardoso-Junior, Boris Yagound, Isobel Ronai, Emily J. Remnant, Klaus Hartfelder, Benjamin P. Oldroyd
Summary: The study found that DNA methylation is not a major factor in driving differential gene expression between tissues or behavioral types, while the presence of a queen affects the expression of all four DNA methyltransferase genes.
Article
Multidisciplinary Sciences
Yao Wang, Chuan Tong, Hanren Dai, Zhiqiang Wu, Xiao Han, Yelei Guo, Deyun Chen, Jianshu Wei, Dongdong Ti, Zongzhi Liu, Qian Mei, Xiang Li, Liang Dong, Jing Nie, Yajing Zhang, Weidong Han
Summary: The study demonstrates that pre-treatment of CAR T cells with a DNA methyltransferase inhibitor enhances anti-tumor activities, cytokine production, and proliferation, allowing for effective eradication of tumors at low doses and improved response to tumor rechallenge.
NATURE COMMUNICATIONS
(2021)
Review
Neurosciences
Lin Qin, Chong Qiao, Volney Sheen, Yu Wang, Jie Lu
Summary: The overexpression of DNMT3L in DS mainly affects global RNA expression of genes rather than DNA methylation, leading to the activation of transcription factors such as HES3, ASCL1, NEUROG2, and CDKN1A, which promotes cell cycle exit and neural differentiation. This phenomenon is independent of DNA methylation and may contribute to the abnormal phenotypes observed in Down syndrome cortex.
PROGRESS IN NEUROBIOLOGY
(2021)
Article
Cell & Tissue Engineering
Kazuki Morita, Akihiro Nakamura, Masakazu Machida, Tomoyuki Kawasaki, Rina Nakanishi, Justin Ichida, Takanori Iwata, Akihiro Umezawa, Hidenori Akutsu
Summary: Chemical inhibition of the NOTCH signaling pathway and DOT1L promotes the generation of hiPSCs from keratinocytes and significantly increases iPSC colony generation from human fibroblast cells via mRNA reprogramming. The user-friendly reprogramming approach using a single-cell sorting system under efficient conditions opens up possibilities for industrial applications in disease modeling and drug screening.
REGENERATIVE THERAPY
(2022)
Article
Cell Biology
Chih-Yi Yang, Rita Jui-Hsien Lu, Ming-Kang Lee, Felix Shih-Hsian Hsiao, Ya-Ping Yen, Chun-Chun Cheng, Pu-Sheng Hsu, Yi-Tzang Tsai, Shih-Kuo Chen, I-Hsuan Liu, Pao-Yang Chen, Shau-Ping Lin
Summary: MSCs lacking DNMT3L exhibit reduced colony forming ability and impaired osteogenesis in vitro, with differentially regulated genes associated with bone morphology-related phenotypes. The observed defects are hypothesized to result from pre-deposited epigenetic signatures from DNMT3L-expressing progenitors, rather than a direct effect of missing DNMT3L in MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Zhixiong Zhang, Guan Wang, Yuyan Li, Dongsheng Lei, Jin Xiang, Liang Ouyang, Yanyan Wang, Jinliang Yang
Summary: DNA methylation is an important epigenetic process that regulates gene expression and has become a promising target for cancer treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Si-Qi Shang, Ying Yang, Xue-Jiao Yang, Xiao-Dong Shi, Yun-Shuo Chen, Yue-Ying Wang
Summary: Metabolic reprogramming occurs in the clonal evolution of acute myeloid leukemia (AML) and affects multiple metabolic pathways, including lipid metabolism. Endothelial lipase (LIPG) may serve as a potential metabolic target for the treatment of AML with abnormal lipid metabolism.
Article
Plant Sciences
Shreya Chowdhury, Ananya Mukherjee, Shrabani Basak, Rohit Das, Arunava Mandal, Pallob Kundu
Summary: The study found that cytosine methylation suppresses TRN1 transcription related to vascular development in fully developed leaves of tomato, and ToLCNDV infection disrupts methylation machinery and reactivates TRN1 expression, likely causing abnormal leaf growth pattern. This finding provides important clues for understanding the pathogenesis and treatment of tomato leaf curl disease.
Article
Biochemistry & Molecular Biology
Jiangtao Ren, Xuhua Zhang, Zhenhui Zhang, Jiafeng Pan, Zhexue Hao, Jin Li, Jun Liu
Summary: The complex and costly manufacturing process of CAR-T cell therapy has hindered its widespread adoption. Induced pluripotent stem cells (iPSCs) have potential as an alternative cellular immunotherapy due to their unlimited self-renewal potential and ability to differentiate into functional immune cells. Careful selection of the original cell for iPSC seed preparation is crucial due to the presence of epigenetic imprint. Our research shows that lymphocyte-origin cells are superior to embryonic stem cells in differentiating into functional immune cells. Blocking Fas-FasL induced apoptosis in T cells significantly improves iPSC generation without affecting their differentiation potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Svetlana Sharifulina, Valentina Dzreyan, Valeria Guzenko, Svetlana Demyanenko
Summary: The study found that the levels and intracellular localization of DNMT1 and G9a proteins changed in penumbra neurons and astrocytes in the rat cerebral cortex after stroke. Inhibition of DNMT1 and G9a protected penumbra cells from apoptosis and reduced the volume of cerebral infarction induced by stroke.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Xin-Bin Zhao, Yun-Ping Chen, Min Tan, Lan Zhao, Yuan-Yuan Zhai, Yan-Ling Sun, Yan Gong, Xi-Qiao Feng, Jing Du, Yu-Bo Fan
Summary: ECM stiffness affects DNA methylation, with a global hypermethylation observed under stiff ECM conditions, particularly in the 5' promoter regions of pluripotent genes. Enhanced DNA methylation is functionally required for the loss of pluripotent gene expression in mESCs grown on stiff ECM. The nuclear transport of DNMT3L is promoted by stiff ECM in a PKC alpha-dependent manner, and DNMT3L binds to Nanog promoter regions during cell-ECM interactions. These findings reveal DNA methylation as a novel target for the mechanical sensing mechanism of ECM stiffness, providing a conserved mechanism for gene expression regulation during cell-ECM interactions.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Oncology
Yue-Xia Chen, Lu-Lu He, Xue-Ping Xiang, Jing Shen, Hong-Yan Qi
Summary: The aim of this study was to investigate the molecular regulatory mechanism of MGMT in NOCs-induced gastric cell malignant transformation and tumorigenesis. The results found that the upregulation of MGMT was induced through DNA promoter hypomethylation. The highly expressed MGMT prevented the NOCs-induced cell malignant transformation and tumorigenesis.
WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY
(2022)
Article
Computer Science, Interdisciplinary Applications
Slo-Li Chu, Kazuhiro Sudo, Hideo Yokota, Kuniya Abe, Yukio Nakamura, Ming -Dar Tsai
Summary: This study proposes a method using several deep learning models to efficiently select the reprogramming cells possibly forming hiPSCs and to predict the shapes of growing hiPSC colonies, with the aim of improving the efficiency when establishing a new hiPSC line culture.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2023)
Article
Gastroenterology & Hepatology
Marina Barcena-Varela, Hannah Paish, Laura Alvarez, Iker Uriarte, Maria U. Latasa, Eva Santamaria, Miriam Recalde, Maria Garate, Alex Claveria, Leticia Colyn, Maria Arechederra, Maria J. Iraburu, Malgorzata Milkiewicz, Piotr Milkiewicz, Bruno Sangro, Stuart M. Robinson, Jeremy French, Ana Pardo-Saganta, Julen Oyarzabal, Felipe Prosper, Krista Rombouts, Fiona Oakley, Jelena Mann, Carmen Berasain, Matias A. Avila, Maite G. Fernandez-Barrena
Summary: The study examined the efficacy of a novel dual reversible G9a/DNMT1 inhibitor CM272 in halting liver fibrogenesis, finding that it inhibited fibrogenic responses and restored normal gene expression and mitochondrial function.