Article
Veterinary Sciences
Yushun Wang, Mincai Nie, Huidan Deng, Siyuan Lai, Yuancheng Zhou, Xiangan Sun, Ling Zhu, Zhiwen Xu
Summary: In this study, a rapid and specific diagnostic method for porcine rotavirus type A (PoRVA) was successfully developed. The method utilized specific primers and probes designed based on the PoRVA VP6 gene, and obtained detection results that were completely consistent with the TaqMan probe-based RT-qPCR method in clinical samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Virology
Moustafa Gibory, Tone Bruun, Elmira Flem, Jennifer Lynn Dembinski, Ildri Haltbakk, Ketil Stordal, Svein Arne Nordbo, Kirsti Jakobsen, Elisebet Haarr, Truls Michael Leegaard, Susanne Gjeruldsen Dudman
Summary: After the introduction of the rotavirus vaccine in Norway, there was no clear evidence of vaccine-induced virus strain replacement, with G1P[8] being the most common type both pre and post vaccine implementation. The study revealed that 21.4% of cases detected were vaccine strains, highlighting the importance of continuous genotype surveillance for assessing vaccine program effectiveness.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Oncology
Dikshat Gopal Gupta, Neelam Varma, Ashish Kumar, Shano Naseem, Man Updesh Singh Sachdeva, Sreejesh Sreedharanunni, Jogeshwar Binota, Parveen Bose, Alka Khadwal, Pankaj Malhotra, Subhash Varma
Summary: This study employed gene expression profiling and proteomic characterization to detect and describe Philadelphia-like B-lineage acute lymphoblastic leukemia in developing countries. The results showed that the median age of Ph-like ALL patients was significantly higher than that of Ph-negative ALL patients, and abnormalities in CRLF2 and JAK2 genes were more common in Ph-like ALL.
Article
Veterinary Sciences
Yangkun Liu, Xueying Han, Xinru Zhang, Jiaxing Liu, Lunguang Yao
Summary: Group A porcine rotavirus (PoRVA) is a significant pathogen causing acute enteritis in piglets worldwide. This study developed a novel and convenient droplet digital PCR (ddPCR) using the conserved region of the VP6 gene for PoRVA detection. The ddPCR exhibited 10 times greater sensitivity than TaqMan real-time quantitative PCR (qPCR), and was highly specific for PoRVA. Clinical sample testing showed a higher positivity rate of ddPCR (5.6%) compared to qPCR (4.4%). The newly developed ddPCR assay can be widely used for clinical diagnosis of PoRVA infections.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Food Science & Technology
Chenang Lyu, Feifeng Lu, Zhentao Shi, Dapeng Wang
Summary: The study developed an ISC-RT-qPCR assay for detecting encapsulated RVA in oysters with higher sensitivity and accuracy than RT-qPCR. When applied to oysters collected from four different farms, ISC-RT-qPCR showed a higher detection rate of RVA compared to RT-qPCR, indicating its potential as a better method for disease monitoring and control.
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Infectious Diseases
Nosheen Basharat, Asma Sadiq, Muhammad Dawood, Shahid Ali, Alam Khan, Rooh Ullah, Hayat Khan, Aamir Aziz, Hamid Ali, Aamer Ali Shah, Ijaz Ali, Jadoon Khan
Summary: This study aimed to detect the prevalence of Rotavirus A (RVA) in children in Pakistan after the introduction of rotavirus vaccines. Results showed a high prevalence of RVA in children with acute gastroenteritis (AGE), with no significant differences in gender and age among RVA infections. The seasons of winter, spring, and fall/autumn were statistically significant for RVA prevalence. Recommendations include implementing rotavirus vaccines, providing adequate nutrition and clean water supply for infants, and continuous surveillance to calculate the disease burden caused by RVA.
BMC INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Paul Wollschlaeger, Daniel Todt, Nadja Gerlitz, Stephanie Pfaender, Thomas Bollinger, Andreas Sing, Alexandra Dangel, Nickolaus Ackermann, Klaus Korn, Armin Ensser, Eike Steinmann, Michael Buhl, Joerg Steinmann
Summary: The study evaluated a commercially available qRT-PCR assay for identifying SARS-CoV-2 B.1.1.7 lineage samples, showing a significant N gene dropout or Ct value shift compared to the S or RdRp gene. The assay reliably discriminated between B.1.1.7 and non-B.1.1.7 positive samples, making it a rapid tool for high-throughput diagnostics.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Agriculture, Dairy & Animal Science
Gazanfar Abass, Zunjar Baburao Dubal, Kaushal K. Rajak, Balasaheb M. Kale, Abhay Raorane, Nitin Dudhe, Bilal Ahmad Malla, Dhananjay Desai, Dharmendra K. Sinha, Obli R. Vinodh Kumar, Yashpal Singh Malik
Summary: This study investigated the genotypes of RVA in pig populations in western and northern India. The results showed the circulation of G4P[6] and G9P[13] RVA strains in pigs, indicating evidence of inter-species transmission with human RVA strains.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Medicine, General & Internal
Dominik Noerz, Moritz Grunwald, Hui Ting Tang, Flaminia Olearo, Thomas Guenther, Alexis Robitaille, Nicole Fischer, Adam Grundhoff, Martin Aepfelbacher, Susanne Pfefferle, Marc Luetgehetmann
Summary: This study aimed to develop a multiplexed Spike-gene SNP detection tool for screening SARS-CoV-2 B.1.617 lineage variants. By incorporating a variety of single nucleotide polymorphism (SNP) assays and multiplex testing, rapid screening for relevant variants was achieved.
Article
Veterinary Sciences
Yuelin Liu, Libing Liu, Jinfeng Wang, Xiaoxia Sun, Yaxin Gao, Wanzhe Yuan, Jianchang Wang, Ruiwen Li
Summary: In this study, two RT-RPA assays (real-time RT-RPA and LFS RT-RPA) were successfully developed for the rapid detection of bovine rotavirus A (BRVA) in cattle fecal samples. The assays showed high specificity, sensitivity, and diagnostic accuracy. The developed RT-RPA assays have great potential for use in under-equipped diagnostic laboratories and point-of-need diagnosis at quarantine stations and farms, which is important for controlling BRVA-associated diarrhea in cattle herds.
BMC VETERINARY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ozer Ates, Kadir Yesilbag
Summary: This study successfully isolated RVA G10P[11] in the cattle farm, and found that this genotype plays an important role in the etiology of diarrhea in newborn calves. It should be considered in the selection of vaccines applied to the dams.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Geum-Young Lee, Kyungmin Park, Young-Sun Lee, Ji Hoon Kim, Kwan Soo Byun, Jongwoo Kim, Won-Keun Kim, Jin-Won Song
Summary: This study evaluated the use of multiplex PCR-based nanopore sequencing to obtain whole-genome sequences of HAV for rapid molecular diagnosis of viral genotypes. The results showed high genome coverage rates (90.4-99.5%) of HAV within 8 hours using singleplex nanopore sequencing. TaqMan qPCR demonstrated the ability to detect and quantify multiple HAV genes. This study provides valuable insights for rapid molecular diagnosis during hepatitis A outbreaks and may enhance public health disease surveillance in the hospital and epidemiology field.
Article
Public, Environmental & Occupational Health
Shoko Okitsu, Pattara Khamrin, Toshiyuki Hikita, Aksara Thongprachum, Ngan Thi Kim Pham, Sheikh Ariful Hoque, Satoshi Hayakawa, Niwat Maneekarn, Hiroshi Ushijima
Summary: The study in Japan showed that with the increase in rotavirus vaccination rates, the detection rates of RVA have decreased, and the genotype combinations have changed in different years.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Biochemical Research Methods
Giovanni Faggioni, Riccardo De Santis, Filippo Moramarco, Mario Di Donato, Angelo De Domenico, Filippo Molinari, Giancarlo Petralito, Claudia Fortuna, Giulietta Venturi, Giovanni Rezza, Florigio Lista
Summary: Yellow fever is a viral disease that can cause severe hemorrhage, but can be controlled through mass immunization campaigns with a safe and effective vaccine. However, the re-emergence of the yellow fever virus since the 1960s requires rapid and specific viral detection methods for timely control measures. A novel molecular assay with high sensitivity and specificity in detecting yellow fever virus strains has been developed.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Infectious Diseases
Anismrita Lahon, Ravi P. Arya, Alexander R. Kneubehl, Megan B. Vogt, Natalie J. M. Dailey Garnes, Rebecca Rico-Hesse
PLOS NEGLECTED TROPICAL DISEASES
(2016)
Article
Immunology
Jennifer L. Spencer, Anismrita Lahon, Linda L. Tran, Ravi P. Arya, Alexander R. Kneubehl, Megan B. Vogt, Daniela Xavier, David R. Rowley, Jason T. Kimata, Rebecca R. Rico-Hesse
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Multidisciplinary Sciences
Kjersti M. Aagaard, Anismrita Lahon, Melissa A. Suter, Ravi P. Arya, Maxim D. Seferovic, Megan B. Vogt, Min Hu, Fabio Stossi, Michael A. Mancini, R. Alan Harris, Maike Kahr, Catherine Eppes, Martha Rac, Michael A. Belfort, Chun Shik Park, Daniel Lacorazza, Rebecca Rico-Hesse
SCIENTIFIC REPORTS
(2017)
Article
Infectious Diseases
Megan B. Vogt, Anismrita Lahon, Ravi P. Arya, Alexander R. Kneubehl, Jennifer L. Spencer Clinton, Silke Paust, Rebecca Rico-Hesse
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Infectious Diseases
Megan B. Vogt, Anismrita Lahon, Ravi P. Arya, Jennifer L. Spencer Clinton, Rebecca Rico-Hesse
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Chemistry, Physical
Chetan Sharma, Nalin H. Maniya, Meghal A. Desai, Sanjaykumar R. Patel
Summary: Porous silicon microparticles were prepared by electrochemical etching process for the study of telmisartan loading and release kinetics. Telmisartan loaded porous silicon microparticles showed higher drug loading compared to thermally oxidized porous silicon, with controlled release observed in the former. Telmisartan-loaded porous silicon microparticles can be a viable option for controlled drug delivery of poorly water-soluble active pharmaceutical ingredients.
Article
Engineering, Electrical & Electronic
Nalin H. Maniya, Divesh N. Srivastava
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
(2020)
Article
Immunology
Ritu Mishra, Anismrita Lahon, Akhil C. Banerjea
JOURNAL OF IMMUNOLOGY
(2020)
Article
Engineering, Chemical
Nalin H. Maniya, Kirti Parashar, Laxman N. Kadam, Divesh N. Srivastava
Summary: The study focuses on developing a cost-effective label-free biosensing platform for highly sensitive and selective detection of the cancer biomarker HSP70. The results confirmed successful surface modifications and immobilization of the anti-HSP70 antibody on the PCE platform, demonstrating great promise for highly sensitive and selective detection of the HSP70 cancer biomarker.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2021)
Article
Immunology
Anismrita Lahon, Ravi P. Arya, Akhil C. Banerjea
Summary: This study investigates the impact of DENV infection on megakaryocyte development and maturation, revealing a crucial role of the PI3K/AKT/mTOR pathway in this process. The findings suggest that DENV impairs this pathway, affecting the development and maturation of megakaryocytes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sneh Lata, Ritu Mishra, Ravi P. Arya, Pooja Arora, Anismrita Lahon, Akhil C. Banerjea, Vikas Sood
Summary: COVID-19, caused by SARS-CoV-2, has resulted in significant social, economic, and human losses worldwide. Understanding the host factors involved in the replication of the virus can aid in the development of novel antiviral therapies and control the viral infection.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sonu Kumar, Nalin Maniya, Ceming Wang, Satyajyoti Senapati, Hsueh-Chia Chang
Summary: This study proposes using a highly atheroprotective subset of HDL (PON1-HDL) as a biomarker for cardiovascular risk assessment. To overcome the complexity caused by interference from HDL-associated substances in existing methods, the researchers developed a new sensor platform (NGEMS) to quantitate PON1-HDL in plasma quickly and accurately within 60 minutes. Experimental results demonstrate the high accuracy of PON1-HDL in diagnosing cardiovascular diseases.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Bogdan Mateescu, Jennifer C. Jones, Roger P. Alexander, Eric Alsop, Ji Yeong An, Mohammad Asghari, Alex Boomgarden, Laura Bouchareychas, Alfonso Cayota, Hsueh-Chia Chang, Al Charest, Daniel T. Chiu, Robert J. Coffey, Saumya Das, Peter De Hoff, Andrew de Mello, Crislyn D'Souza-Schorey, David Elashoff, Kiarash R. Eliato, Jeffrey L. Franklin, David J. Galas, Mark B. Gerstein, Ionita H. Ghiran, David B. Go, Stephen Gould, Tristan R. Grogan, James N. Higginbotham, Florian Hladik, Tony Jun Huang, Xiaoye Huo, Elizabeth Hutchins, Dennis K. Jeppesen, Tijana Jovanovic-Talisman, Betty Y. S. Kim, Sung Kim, Kyoung-Mee Kim, Yong Kim, Robert R. Kitchen, Vaughan Knouse, Emily L. LaPlante, Carlito B. Lebrilla, L. James Lee, Kathleen M. Lennon, Guoping Li, Feng Li, Tieyi Li, Tao Liu, Zirui Liu, Adam L. Maddox, Kyle McCarthy, Bessie Meechoovet, Nalin Maniya, Yingchao Meng, Aleksandar Milosavljevic, Byoung-Hoon Min, Amber Morey, Martin Ng, John Nolan, Getulio P. De Oliveira Junior, Michael E. Paulaitis, Tuan Anh Phu, Robert L. Raffai, Matthew E. Roth, David A. Routenberg, Joel Rozowsky, Joseph Rufo, Satyajyoti Senapati, Sigal Shachar, Himani Sharma, Anil K. Sood, Stavros Stavrakis, Alessandra Sturchler, Muneesh Tewari, Juan P. Tosar, Alexander K. Tucker-Schwartz, Andrey Turchinovich, Nedyalka Valkov, Kendall Van Keuren-Jensen, Kasey C. Vickers, Lucia Vojtech, Wyatt N. Vreeland, Ceming Wang, Kai Wang, ZeYu Wang, Joshua A. Welsh, Kenneth W. Witwer, David T. W. Wong, Jianping Xia, Ya-Hong Xie, Kaichun Yang, Mikolaj P. Zaborowski, Chenguang Zhang, Qin Zhang, Angela M. Zivkovic, Louise C. Laurent
Summary: The extracellular RNA communication consortium (ERCC) is a NIH-funded program that aims to advance the development of new technologies, resources, and knowledge about exRNAs and their carriers. Through its second phase (ERCC2), the program focuses on addressing important gaps in knowledge and technology, as well as promoting collaboration between ERCC2 members and the scientific community. The ultimate goal is to enhance our understanding of exRNA biology and improve the development of accurate and efficient exRNA-based diagnostic methods.
Proceedings Paper
Energy & Fuels
Nalin H. Maniya, Jibinlal Ashokan, Divesh N. Srivastava
INTERNATIONAL CONFERENCE ON NANOMATERIALS FOR ENERGY CONVERSION AND STORAGE APPLICATIONS (NECSA 2018)
(2018)
Article
Nanoscience & Nanotechnology
Nalin H. Maniya
REVIEWS ON ADVANCED MATERIALS SCIENCE
(2018)
