Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 53, Issue 5, Pages 345-350Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2013.07.007
Keywords
Bradyrhizobium japonicum; Nitrate reductase; Glutathione; Cadmium
Categories
Funding
- Secretaria de Ciencia y Tecnica de la Universidad Nacional de Rio Cuarto (SECYT-UNRC)
- CONICET-MinCyt (Cordoba)
- CONICET
Ask authors/readers for more resources
In this work, we evaluated the effects of cadmium (Cd) on the antioxidant defense system responses and the role of nitrate reductase (NR) in the redox balance maintenance in Bradyrhizobium japonicum strains. For that, B. japonicum USDA110 and its NR defective mutant strain (GRPA1) were used. Results showed that the addition of 10 mu M Cd did not modify the aerobic growth of the wild type strain while the mutant strain was strongly affected. Anaerobic growth revealed that only the parental strain was able to grow under this condition. Cd reduced drastically the NR activity in B. japonicum USDA110 and increased lipid peroxide content in both strains. Cd decreased reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio in B. japonicum USDA110 although, a significant increased was observed in the mutant GRPA1. GSH-related enzymes were induced by Cd, being more evident the increase in the mutant strain. This different behavior observed between strains suggests that NR enzyme plays an important role in the redox balance maintenance in B. japonicum USDA 110 exposed to Cd. (c) 2013 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available