Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 53, Issue 2, Pages 110-117Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2013.04.005
Keywords
Compressed carbon dioxide; Pseudomonas cepacia lipase (PCL); Fourier transform-infrared spectrometry (FT-IR); Fluorescence spectra; Organic solvent tolerance; Thermal stability
Categories
Funding
- National Natural Science Foundation of PR China (NSFC) [31070089, 31170078, J1103514]
- National High Technology Research and Development Program of PR China (863 Program) [2011AA02A204]
- Innovation Foundation of HUST
Ask authors/readers for more resources
In this work, we have investigated the influences of sub- and supercritical CO2 treatment on the properties of Pseudomonas cepacia lipase (PCL), including its esterification and transesterification activities, structural changes and stability. Results demonstrated that exposure time to subcritical CO2 treatment had a negative effect on PCL transesterification activity whereas exposure time to supercritical CO2 treatment had a positive effect. But generally, most compressed treatments significantly enhanced PCL esterification activity. Conformational analysis by FT-IR and fluorescence emission spectra revealed that enhanced activities after supercritical CO2 treatment were correlated with the secondary and tertiary structural changes of PCL. Secondary structure changes also appeared to be responsible for enhancement of PCL activities by subcritical CO2 treatment. Compared to native PCL, treated PCL's esterification activity significantly decreased in hydrophilic organic media, while transesterification activity significantly increased in tert-amyl alcohol and acetone. After supercritical treatment, the thermal stability of PCL significantly decreased in esterification reactions, however, there was no significant difference in transesterification reactions. (C) 2013 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available