Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 37, Issue 3, Pages 1157-1168Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2014.04.002
Keywords
AHR2; PXR; Cytochrome P450; Sulfotransferase; UDP-glucuronosyltransferases; 17Alpha-methyltestosterone
Funding
- National Natural Science Foundation of China [31270547]
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17Alpha-methyltestosterone (MT), a synthetic androgen, is widely used in aquaculture. Aquatic organisms can receive continuous exposure to residual MT throughout their lives. Aiming to evaluate the effects of MT on genes involved in biotransformation pathway, meanwhile attempting to unravel the MT metabolic pathway at the transcriptional level in fish, here we isolated the cDNAs of previously unreported AHR2, Sult1 st1, Ugt2a1 and Ugt2b6 in rare minnow, and predominantly investigated the hepatic transcriptional patterns of AHR2, PXR and five biotransformation genes after MT exposure in both genders adult rare minnow Gobiocypris rarus. The present findings suggest that AHR2 and PXR should play important roles in regulating biotransformation enzymes related to MT catabolism, moreover, CYP1A, CYP3A, SULT1 ST4, SULT1 ST6 and UGT2A1 may play certain roles in catabolism of MT in adult G. rarus. Additionally, UGT2A1 may make greater contribution than SULT1 ST4 and SULT1 ST6 in MT catabolism in males. (C) 2014 Elsevier B.V. All rights reserved.
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