Article
Endocrinology & Metabolism
Rikke Poulsen, Shireen H. Jackman, Martin Hansen, Caren C. Helbing
Summary: Anuran metamorphosis is associated with changes in thyroid hormone levels and gene expression. New technology allows for measuring TH levels and gene transcription during metamorphosis. The concentration profile of T2 during metamorphosis suggests its role in anuran development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Engineering, Environmental
Cornelia Kienle, Inge Werner, Stephan Fischer, Christina Luthi, Andrea Schifferli, Harrie Besselink, Miriam Langer, Christa S. McArdell, Etienne L. M. Vermeirssen
Summary: Ozonation can effectively eliminate micropollutants in wastewater, but it may also produce reactive and potentially toxic transformation products. Additional post-treatments are recommended to further remove these products. The study found that ozonation reduced ecotoxicological effects, and post-treatments further reduced these effects. Rainbow trout liver gene expression and algal photosynthesis were sensitive indicators for evaluating the treatment efficiency.
Article
Biochemistry & Molecular Biology
Eric L. Bolf, Noelle E. Gillis, Cole D. Davidson, Lauren M. Cozzens, Sophie Kogut, Jennifer A. Tomczak, Seth Frietze, Frances E. Carr
Summary: This study found that restoring TR beta expression in breast cancer cells resulted in more epithelial cell characteristics, reduced expression of mesenchymal cytokeratins, and decreased epithelial to mesenchymal transition pathway activity. Additionally, comparison with thyroid cancer cells revealed shared gene regulation by TR beta in pathways such as lipid metabolism and chromatin remodeling.
MOLECULAR CARCINOGENESIS
(2021)
Article
Environmental Sciences
Lauris Evariste, Antoine Mottier, Eric Pinelli, Emmanuel Flahaut, Laury Gauthier, Florence Mouchet
Summary: The interest in graphene-based nanomaterials (GBMs) is increasing globally due to their innovative applications, but concerns about their potential adverse effects on the environment and endocrine disruption potential are also growing. A study found that graphene oxide (GO) and reduced graphene oxide (rGO) have different effects on amphibian endocrine systems, with GBMs potentially increasing the bioavailability and toxicity of other pollutants through hormone adsorption. This highlights the need for further research on the environmental impact and potential risks associated with GBMs.
Article
Engineering, Environmental
Jian Li, Ying Xu, Na Li, Rui Zuo, Yuanzheng Zhai, Haiyang Chen
Summary: Through integrated in vitro, in vivo, and in silico assays, the study investigated the thyroid hormone-disrupting effects and mechanisms of 8 typical organophosphate esters (OPEs). It was found that these chemicals competitively bound to the membrane thyroid hormone receptor (mTR) and some OPEs also interacted with the nuclear thyroid hormone receptor (nTR). Molecular docking simulation supported these findings. In addition, in vivo analyses using zebrafish embryo development demonstrated the thyroid hormone-disrupting effects of OPEs and their possible association with mTR.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Review
Environmental Sciences
Jana Cahova, Jana Blahova, Jan Mares, Nikola Hodkovicova, Pavel Sauer, Hana Kocour Kroupova, Zdenka Svobodova
Summary: This study investigates the impact of the ultraviolet filter octinoxate on thyroid hormone regulation in rainbow trout. The results indicate that octinoxate can disrupt thyroid hormone regulation, leading to imbalances in the organism. Further research is needed to fully understand the regulatory mechanism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Jinbo Li, Yuanyuan Li, Min Zhu, Shilin Song, Zhanfen Qin
Summary: In this study, a rapid enzyme-linked immunosorbent assay (ELISA) was developed to screen thyroid hormone signaling disruptors. The T3 concentration was first screened, followed by testing two known disruptors and detecting several suspected disruptors. The results demonstrated that TH signaling disruptors can be rapidly screened out by evaluating the expression of the thibz gene as a sensitive endpoint.
Article
Environmental Sciences
Chao Hu, Yeqing Xu, Mingmin Wang, Shixuan Cui, Hangjun Zhang, Liping Lu
Summary: This study evaluated the disruption of thyroid endocrine by bisphenol analogues in mice, and found that these compounds can lead to thyroid dysfunction and glandular structural damage, further affecting the synthesis of thyroid hormones and physiological processes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Ichiro Yamauchi, Yoriko Sakane, Takafumi Yamashita, Takuro Hakata, Taku Sugawa, Haruka Fujita, Kentaro Okamoto, Daisuke Taura, Keisho Hirota, Yohei Ueda, Toshihito Fujii, Akihiro Yasoda, Nobuya Inagaki
Summary: This study aimed to investigate the effects of peripheral iodothyronine deiodinases (DIOs) on thyroid hormone economy. The results showed that D2 could enhance local thyroid hormone action and promote its circulation, while D3 could decrease circulating T3 and T4 levels with elevated reverse T3, leading to consumptive hypothyroidism. Additionally, the D3 mice are expected to be a novel hypothyroidism model.
Article
Biochemistry & Molecular Biology
Huei-Pin Lai, Mei-Hsiu Chen
Summary: This study reports a family with resistance to thyroid hormone (RTH) caused by a rare point mutation in the thyroid hormone receptor beta (THR beta) gene. The proband developed atrial fibrillation and life-threatening heart failure with pulmonary edema, which differs from previously reported mutations. This study contributes to a better understanding and management of patients with RTH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Carla Moran, Nadia Schoenmakers, David Halsall, Susan Oddy, Greta Lyons, Sjoerd van den Berg, Mark Gurnell, Krishna Chatterjee
Summary: This article describes a diagnostic approach to discordant thyroid function tests, focusing on factors causing elevated free thyroxine and/or free triiodothyronine measurements with nonsuppressed TSH levels. A structured approach combining clinical assessment with additional laboratory investigations can establish a correct diagnosis, potentially preventing unnecessary investigation or inappropriate therapy.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Pharmacology & Pharmacy
Lin Wang, Mei Guo, Guoli Feng, Pan Wang, Jie Xu, Jie Yu
Summary: Chronic exposure to nonylphenol at environmental concentration may have toxic effects on thyroid function and induce thyroid hyperplasia disease in male rats.
Review
Environmental Sciences
Krishnan Suresh Kumar, Subbiah Kavitha, Kalivel Parameswari, Ayyasamy Sakunthala, Palanivel Sathishkumar
Summary: Perchlorate, a strong oxidizer, is an emerging contaminant that has gained attention due to its occurrence, reactive nature, and persistence in various environments. It is used in industries such as missile fuel and fertilizers. Ingesting perchlorate through water or food can negatively affect thyroid hormone synthesis, which is crucial for metabolism and neural development. Current remediation methods involve physical-chemical techniques, but alternative treatments are needed due to waste generation. This review discusses hybrid technologies that are being researched for the treatment of perchlorate and other emerging contaminants.
Article
Biotechnology & Applied Microbiology
Asmita Gupta, Madan Kumar, Pooja Ghosh, Swati, Indu Shekhar Thakur
Summary: The present study conducted physico-chemical profiling and toxicity assessment of wastewater and sludge samples from a municipal wastewater treatment plant. The results showed that the post-treatment liquid samples met discharge standards but still contained organic contaminants, suggesting the need for improvement in testing and treatment technologies.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Endocrinology & Metabolism
Georg Sebastian Hoenes, Helena Kerp, Christoph Hoppe, Manuela Kowalczyk, Denise Zwanziger, Hideo Andreas Baba, Dagmar Fuehrer, Lars Christian Moeller
Summary: This study found that DNA binding of TR beta is required for T3-induced stimulation of hepatocyte proliferation through the Wnt/beta-catenin pathway. Instead of directly activating Wnt signaling, T3 and TR beta induce key genes of the Wnt/beta-catenin pathway, ultimately stimulating hepatocyte proliferation via CyclinD1.
Article
Environmental Sciences
Jody L. Heerema, Caren C. Helbing, Greg G. Pyle
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2018)
Article
Behavioral Sciences
Jody L. Heerema, Kevin W. Jackman, Rachel C. Miliano, Linda Li, Tristan S. M. Zaborniak, Nik Veldhoen, Graham van Aggelen, Wayne J. Parker, Gregory G. Pyle, Caren C. Helbing
HORMONES AND BEHAVIOR
(2018)
Article
Biochemical Research Methods
Anna Perzanowska, Agnieszka Fatalska, Grzegorz Wojtas, Andrzej Lewandowicz, Agata Michalak, Grzegorz Krasowski, Christoph H. Borchers, Michal Dadlez, Dominik Domanski
PROTEOMICS CLINICAL APPLICATIONS
(2018)
Article
Engineering, Environmental
Jacob J. Imbery, Craig Buday, Rachel C. Miliano, Dayue Shang, Jessica M. Round, Honoria Kwok, Graham Van Aggelen, Caren C. Helbing
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Jared Hobbs, Jessica M. Round, Michael J. Allison, Caren C. Helbing
Article
Multidisciplinary Sciences
Caren C. Helbing, S. Austin Hammond, Shireen H. Jackman, Simon Houston, Rene L. Warren, Caroline E. Cameron, Inanc Birol
SCIENTIFIC REPORTS
(2019)
Article
Oncology
Agnieszka Fatalska, Natalia Rusetska, Elwira Bakula-Zalewska, Artur Kowalik, Sebastian Zieba, Agnieszka Wroblewska, Kamil Zalewski, Krzysztof Goryca, Dominik Domanski, Magdalena Kowalewska
Summary: Our study aimed to identify protein markers of VSCC tumors for patient stratification based on progression risk. Mass spectrometry-based proteomics revealed immune response as the most over-represented category for differentially expressed proteins (DEPs) in progVSCC vs. d-fVSCC tumors, with pathway profiling indicating bacterial infections linked to aggressive VSCC phenotypes. HMGA2 and PRTN3 were identified as proteins predicting VSCC progression and show promise as markers for patient stratification. Bacterial infections triggering an inflammatory response seem to contribute to cancer progression in VSCC, suggesting that targeted therapies should consider bacterial rather than viral infection status.
Article
Environmental Sciences
Lorissa M. Corrie, Meaghan N. Kempe, Oxana Blajkevitch, Dayue Shang, Caren C. Helbing
Summary: The study shows that dioctyl sodium sulfosuccinate (DOSS) can disrupt thyroid hormone (TH) signaling in American bullfrogs, affecting their developmental process. DOSS disrupted the expression of TH-responsive genes in liver and tail fin tissue of tadpoles, with different patterns of effects observed at various concentrations.
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2021)
Article
Environmental Sciences
Ethan A. Abbott, Caren C. Helbing
Summary: The study found that sucralose altered the expression of fundamental thyroid hormone-response genes in premetamorphic American bullfrog tadpoles, indicating potential impacts of environmental sucralose exposure on amphibian metamorphosis. Further investigations into the cumulative effects of sucralose exposure are warranted.
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2021)
Article
Environmental Sciences
Anita A. Thambirajah, Michael G. Wade, Jonathan Verreault, Nicolas Buisine, Veronica A. Alves, Valerie S. Langlois, Caren C. Helbing
Summary: This critical review highlights the complexity of Endocrine Disrupting Chemicals interfering with Thyroid Hormone function through interactions with other hormonal axes, and emphasizes the lack of data generated in non-mammalian vertebrate classes. With the availability of more genomics-based resources, better identification and delineation of EDC effects, modes of action, and identification of effective biomarkers suitable for HPT disruption is possible. The effects of EDCs are likely to cascade into a plurality of complex physiological effects, beyond the few variables tested within research studies.
ENVIRONMENTAL RESEARCH
(2022)
Article
Infectious Diseases
Diana Lin, Darcy Sutherland, Sambina Islam Aninta, Nathan Louie, Ka Ming Nip, Chenkai Li, Anat Yanai, Lauren Coombe, Rene L. Warren, Caren C. Helbing, Linda M. N. Hoang, Inanc Birol
Summary: Antibiotic resistance is a global health crisis, and alternative antimicrobial therapeutics are urgently needed. This study presents rAMPage, a bioinformatics discovery platform, to identify AMP sequences from RNA-seq datasets. The researchers used rAMPage on 84 publicly available RNA-seq datasets, identifying 1137 possible AMPs, with 1024 deemed novel. Testing 21 selected peptide sequences, seven of them showed high antimicrobial activity.
Article
Infectious Diseases
Amelia Richter, Darcy Sutherland, Hossein Ebrahimikondori, Alana Babcock, Nathan Louie, Chenkai Li, Lauren Coombe, Diana Lin, Rene L. Warren, Anat Yanai, Monica Kotkoff, Caren C. Helbing, Fraser Hof, Linda M. N. Hoang, Inanc Birol
Summary: Antimicrobial peptides (AMPs) are promising biological molecules for developing new therapeutics against antimicrobial resistance. In this study, 51 AMP candidates were bioinformatically identified and characterized. These AMPs demonstrated activity against bacterial pathogens and low toxicity to human cells. Sequence and structural analysis also predicted their antimicrobial activity.
Article
Marine & Freshwater Biology
Anita A. Thambirajah, Rachel C. Miliano, Ethan A. Abbott, Craig Buday, Dayue Shang, Honoria Kwok, Caren C. Helbing
Summary: Oil spills in high traffic coastal environments have significant impacts on marine ecosystems and the dependent commercial and social interests. It is crucial to develop effective tools to monitor oil contamination and remediation in a timely manner due to the ongoing reliance on marine fuels. This study demonstrates the value of using the cyp1a1 transcript as a sensitive indicator for tracking exposure and recovery from oil contamination.
AQUATIC TOXICOLOGY
(2023)
Article
Fisheries
Morgan D. Hocking, Jeffrey C. MacAdams, Michael J. Allison, Lauren C. Bergman, Robert Sneiderman, Ben F. Koop, Brian M. Starzomski, Mary L. Lesperance, Caren C. Helbing
Summary: A current challenge in environmental DNA (eDNA) applications is accounting for environmental background in surveys. This study conducted two controlled experiments using a coho salmon eDNA assay, demonstrating a novel method to detect target species eDNA in the presence of background and providing a statistically robust assessment for eDNA surveys.
Article
Biology
Michael J. Allison, Jessica M. Round, Lauren C. Bergman, Ali Mirabzadeh, Heather Allen, Aron Weir, Caren C. Helbing
Summary: The study evaluated the quality of filter-immobilized eDNA desiccated with silica gel under different storage conditions for over a year using targeted quantitative real-time polymerase chain reaction (qPCR)-based assays. The results showed that silica gel outperformed ethanol preservation at preventing a decrease in eDNA sample quality at higher temperatures, and that long-term storage of eDNA filters with silica gel beads should be at -20 degrees C to maintain sample integrity.
BMC RESEARCH NOTES
(2021)