Article
Biotechnology & Applied Microbiology
Kamran Jawed, Victor Uhunoma Irorere, Rajesh Reddy Bommareddy, Nigel P. Minton, Katalin Kovacs
Summary: The facultative chemolithoautotroph Cupriavidus necator H16 can grow aerobically with organic substrates or H-2 and CO2, and accumulate a large amount of poly (3-hydroxybutyrate) (PHA). This study investigated the ability of this organism to co-utilize volatile fatty acids (VFAs) and CO2 for carbon sources under mixotrophic growth conditions, and monitored PHA production. The results showed that PHA accumulation was significantly higher when cultures were grown mixotrophically with H-2 as an additional energy source. A two-step VFA feeding regime using a VFA mixture derived from an anaerobic digestor (AD) was found to be the most effective method for PHA accumulation.
FERMENTATION-BASEL
(2022)
Article
Agricultural Engineering
Juhi Gupta, Rashmi Rathour, Neha Maheshwari, Indu Shekhar Thakur
Summary: The study demonstrated the enhanced production capability of strain Cupriavidus sp. ISTL7 for polyhydroxyalkanoates using acetate and glucose, with a need for optimization of solvent extraction and energy consumption for sustainable production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Der-Shyan Sheu, Ji-Long Chen, Shih-Yi Sheu, Wann-Neng Jane
Summary: By deleting the wcaJ gene, this study effectively addressed the issue of mucoid-colony formation in Cupriavidus sp. L7L fermentation, increased PHA production and improved the efficiency of Lev-to-PHA conversion, making cell harvest easier.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biotechnology & Applied Microbiology
Le Zhang, Zicheng Jiang, To-Hung Tsui, Kai-Chee Loh, Yanjun Dai, Yen Wah Tong
Summary: This article reviews the latest technologies and strategies for poly(3-hydroxybutyrate) (PHB) production via Cupriavidus necator fermentation. It assesses the biosynthesis pathway mechanism of PHB production, summarizes and analyzes the efficiency of common carbon sources. The key strategies for enhancing PHB production in recent years are also summarized, with technical challenges and future prospects highlighted.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hisashi Arikawa, Shunsuke Sato
Summary: This study focuses on the functionality of specific genes in relation to 3HHx monomer supply in PHBHHx production. The results show that specific beta-ketothiolases, BktB and H16_A1528, play an important role in C6 metabolism. Additionally, overexpression of (R)-specific enoyl-CoA hydratase and PHA synthase significantly increases the proportion of 3HHx in PHBHHx produced from plant oil.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Estel Rueda, Beatriz Altamira-Algarra, Joan Garcia
Summary: The effect of four parameters on PHB production in Synechococcus sp. and Synechocystis sp. was tested and optimized. Different optimal conditions were found for the two strains.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Hannes Loewe, Marleen Beentjes, Katharina Pflueger-Grau, Andreas Kremling
Summary: In this study, it was found that the hydrogen-oxidizing bacterium C. necator is a natural producer of trehalose under stress conditions, and through genetic engineering, the trehalose production can be further enhanced. By utilizing different substrates, high trehalose yields can be achieved.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Bhawna Tyagi, Bulbul Gupta, Deepak Khatak, Ramovatar Meena, Indu Shekhar Thakur
Summary: In this study, Methylobacterium sp. ISTM1 was able to produce both EPS and PHA simultaneously in a single-step process by optimizing the culture conditions. The genomic analysis identified the genes and pathways involved in EPS and PHA production. The non-toxicity of the biopolymers was confirmed by cytotoxicity analysis. This research demonstrates the potential of utilizing agro-industrial waste for sustainable production of non-toxic biopolymers by Methylobacterium sp. ISTM1.
BIORESOURCE TECHNOLOGY
(2022)
Article
Materials Science, Paper & Wood
Wei-Lin Tu, Hsiao-Kai Chu, Chun-Mei Huang, Chia-Hsin Chen, Chung-Mao Ou, Gia-Luen Guo
Summary: Biodegradable polymers, such as polyhydroxyalkanoates (PHAs), are promising alternatives to petroleum-based plastics because of their lower environmental impact. Research shows that using different carbon sources for PHA production in cultured bacteria can reduce costs and production time without affecting the structure of the PHA. These results provide valuable insights for future applications of PHA production with other bacteria.
Article
Agricultural Engineering
Shashi Kant Bhatia, Vishal Ahuja, Neha Chandel, Ranjit Gurav, Ravi Kant Bhatia, Muthusamy Govarthanan, Vinay Kumar Tyagi, Vinod Kumar, Arivalagan Pugazendhi, J. Rajesh Banu, Yung-Hun Yang
Summary: Urbanization and pollution are major issues in today's world due to the consumption of fossil fuels. Algae provides a renewable substitute for energy and has commercial value. Algal extracts contain bioactive compounds with various potentials such as antimicrobial, anti-oxidative, anti-inflammatory, and anti-cancer properties. Algal biomass can be processed for sugar recovery and used to produce bioenergy and biochemicals through microbial fermentation.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Alexander Kettner, Matthias Noll, Carola Griehl
Summary: Fluorescence spectroscopy combined with side scatter was used to monitor the biomass and poly(3-hydroxybutyrate) (PHB) formation in biotechnological applications. Different dyes were compared for PHB quantification, and the results showed high accuracy and transferability. This approach provides a fast and simultaneous detection method for monitoring biomass and PHB content in fermentation processes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Priyam Tyagi, Aarti Sharma
Summary: The study demonstrated that untreated paper industry effluent can be utilized as a substrate for Polyhydroxyalkanoates (PHA) production, utilizing unused carbon and developing a feasible production process. By screening indigenous PHA-producing bacterial strains, it was found that ART_41 was able to grow using different concentrations of effluent, leading to optimal PHA production after identification and analysis.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Biotechnology & Applied Microbiology
Vera Lambauer, Alexander Permann, Zdenek Petrasek, Vanja Subotic, Christoph Hochenauer, Regina Kratzer, Markus Reichhartinger
Summary: Gas fermentation is a promising technology that converts gaseous substrates into valuable products using autotrophic microorganisms. In this study, a standard benchtop bioreactor was redesigned for the growth of Cupriavidus necator on explosive mixtures of CO2, H-2, and O-2. The bioreactor was equipped with mass flow control units and gas sensors, and an automated gas dosage controller was developed. The dissolved O-2 concentrations were adjusted with high precision, resulting in the production of up to 15 g/L cell dry weight.
FERMENTATION-BASEL
(2023)
Article
Engineering, Environmental
Hue-Ngan Dai, Chin-Tsan Wang, Ba-Tam Tran, Tam-Anh Duong Nguyen, Loan-Phung My Le, Man Van Tran
Summary: This study compares the performance of bufferless microbial fuel cells (MFCs) to those that employ buffers for wastewater treatment. The findings reveal that bufferless MFCs have a higher power generation and current density, although slightly lower COD removal percentage compared to buffered MFCs.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Ivana Novackova, Vendula Hrabalova, Eva Slaninova, Petr Sedlacek, Ota Samek, Martin Koller, Vladislav Krzyzanek, Kamila Hrubanova, Katerina Mrazova, Jana Nebesarova, Stanislav Obruca
Summary: Polyhydroxyalkanoates (PHA) are microbial polyesters that serve as storage compounds for carbon and energy. In addition to their storage function, PHA also plays a role in enhancing microbial cells' resistance to various stressors. This study investigates the role of PHA in Cupriavidus necator, a model organism for PHA metabolism, in adapting to osmotic pressure and copper ions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Energy & Fuels
Kawinda Sriyod, Alissara Reungsang, Pensri Plangklang
Summary: The study investigated the saccharification of Chlorella sp. biomass using single and combined commercial carbohydrase enzymes, demonstrating that a combined enzyme treatment led to increased reducing sugar yield and hydrolysis efficiency, as well as improved hydrogen production process.
BIOENERGY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Parichat Phalanisong, Pensri Plangklang, Alissara Reungsang
Summary: Microalgae consortia were cultivated in SBPRs with altered growth phases to select consortia capable of PHA accumulation. The selected consortia showed high PHA contents when supplemented with sugar sources, with potential for biomass and PHA production. This mixed-culture non-sterile system provides valuable insights for large-scale microalgal PHA production.
Article
Biochemistry & Molecular Biology
Sarinthip Thanakkasaranee, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Pensak Jantrawut, Warintorn Ruksiriwanich, Sarana Rose Sommano, Winita Punyodom, Alissara Reungsang, Thi Minh Phuong Ngo, Parichat Thipchai, Wirongrong Tongdeesoontorn, Pornchai Rachtanapun
Summary: This study found that using chitosan powder with smaller particle sizes can significantly improve the water solubility of the CMCh powders and films. Additionally, as the particle size of chitosan decreased, the mechanical properties of the CMCh films also increased.
Article
Polymer Science
Thi Minh Phuong Ngo, Thanh Hoi Nguyen, Thi Mong Quyen Dang, Thi Van Thanh Do, Alissara Reungsang, Nareekan Chaiwong, Pornchai Rachtanapun
Summary: The study revealed that the pectin/nanochitosan coating at a low temperature (17 degrees C) had the best effect on preserving and maintaining the weight of mango fruits, thus extending their shelf-life and maintaining quality effectively.
Article
Biochemistry & Molecular Biology
Kittisak Jantanasakulwong, Sarinthip Thanakkasaranee, Phisit Seesuriyachan, Pisith Singjai, Aphisit Saenjaiban, Siriphan Photphroet, Kanticha Pratinthong, Yuthana Phimolsiripol, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Sommano, Pensak Jantrawut, Siriwadee Chomdej, Suwit Chotinan, Francisco J. Barba, Joe M. Regenstein, Alissara Reungsang, Pornchai Rachtanapun
Summary: The nano-metal-treated PET films with anti-virus and anti-fogging ability were developed using sparking nano-metal particles of Ag, Zn, and Ti wires on polyethylene terephthalate (PET) films. A highly transparent, anti-fogging, and anti-virus face shield was prepared using the Zn-PET film. Sparking was an effective technique to prepare the alternative anti-virus and anti-fogging films for medical biomaterial applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kamolwan Thepsuthammarat, Alissara Reungsang, Pensri Plangklang
Summary: Improving biomass production by using low-cost substrates is important for large-scale microalgae production. In this study, the microalga Coelastrella sp. KKU-P1 was cultivated mixotrophically using unhydrolyzed molasses as a carbon source. The optimal conditions for maximizing biomass production were determined, and a batch cultivation achieved the highest biomass production of 3.81 g/L. CO2 supplementation did not improve biomass production, indicating that ambient CO2 concentration was sufficient for mixotrophic growth. The biochemical analysis showed that the microalgal biomass has potential as a source of essential amino acids, pigments, and fatty acids.
Article
Biotechnology & Applied Microbiology
Gede Adi Wiguna Sudiartha, Tsuyoshi Imai, Chonticha Mamimin, Alissara Reungsang
Summary: Temperature plays a significant role in anaerobic digestion, affecting microbial communities and process efficiency. This study tested two temperature shift scenarios and found that both led to a decrease in biogas production, with the upshifted scenario experiencing a larger decrease. 16S rRNA sequencing revealed that the dominant methanogens differed between the two scenarios, indicating that temperature shifts affect microbial communities and biogas production.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Sureewan Sittijunda, Sulfan Baka, Rattana Jariyaboon, Alissara Reungsang, Tsuyoshi Imai, Prawit Kongjan
Summary: This study aimed to enhance dark fermentative hydrogen production from co-digestion of distillery wastewater and glycerol waste. The optimal ratio of DW and GW for hydrogen production was found to be 99:1, resulting in a hydrogen yield of 149.5 mL-H-2/g - VSadded. The dark fermentation effluent was then used for methane production, with a maximum methane yield of 115.1 mL-CH4/g - VSadded. Microbial community analysis revealed the presence of methanogenic archaea in the microbial electrolysis cell reactor.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Marika Ngamsirisomsakul, Mallika Boonmee Kongkeitkajorn, Sittipong Amnuaypanich, Alissara Reungsang
Summary: Sugarcane bagasse can be used as a raw material for microbial lipid production, and incorporating glycerol into unconcentrated bagasse hydrolysate helps improve growth and lipid production in Rhodotorula glutinis TISTR 5159. When mixed with 10% v/v glycerol, the hydrolysate with yeast extract as the sole nitrogen source achieved the highest lipid yield.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Khamanitjaree Saripan, Chonticha Mamimin, Tsuyoshi Imai, Sureewan Sittijunda, Alissara Reungsang
Summary: This study aims to create a q-PCR methodology for monitoring microbial communities in hydrogen-producing mixed cultures and develop specific primers for quantitative analysis of hydrogen-producing bacteria. The dominant bacteria in the enriched thermophilic mixed cultures were identified as Clostridium sp. and Thermoanaerobacterium sp., and their 16S rRNA gene copy number increased with time during hydrogen production.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Apik Khautsart Miftah, Sureewan Sittijunda, Tsuyoshi Imai, Apilak Salakkam, Alissara Reungsang
Summary: This study determined the optimal conditions for the pretreatment of sugarcane leaves with deep eutectic solvent (DES), and found the best fermentation mode for hydrogen and methane production from DES-pretreated sugarcane leaves. The results showed that the simultaneous saccharification and fermentation (SSF) process was more efficient than the separate hydrolysis and fermentation (SHF) process in both two-stage hydrogen and methane production process and one-stage methane production process.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Sreyden Hor, Mallika Boonmee Kongkeitkajorn, Alissara Reungsang
Summary: This study investigates the biorefinery of sugarcane bagasse into ethanol and xylitol. The results show that ethanol fermentation of sugarcane bagasse hydrolysate can be carried out without supplementing a nitrogen source, and approximately 50 g/L of bioethanol can be produced after 36 hours of fermentation. The vinasse containing xylose can be successfully used to produce xylitol, and supplementing the vinasse with yeast extract improves xylitol production. Controlled dissolved oxygen and the addition of low fraction of molasses do not positively affect xylitol production.
FERMENTATION-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Pornchai Rachtanapun, Sarinthip Thanakkasaranee, Rafael A. Auras, Nareekan Chaiwong, Kittisak Jantanasakulwong, Pensak Jantrawut, Yuthana Phimolsiripol, Phisit Seesuriyachan, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Somman, Warintorn Ruksiriwanich, Warinporn Klunklin, Alissara Reungsang, Thi Minh Phuong Ngo
Summary: The effect of sodium hydroxide concentrations on the morphology, mechanical properties, and water barrier properties of carboxymethyl rice starch films was investigated. Increasing NaOH concentrations resulted in higher degree of substitution of CMSr powders, leading to changes in morphology. The water solubility, elongation at break, and water vapor permeability of the films increased as NaOH concentrations increased, while the water contact angle, melting temperature, and tensile strength decreased.
Article
Biotechnology & Applied Microbiology
Sureewan Sittijunda, Napapat Sitthikitpanya, Pensri Plangklang, Alissara Reungsang
Summary: Optimization of factors affecting biohydrogen production through anaerobic digestion of crude glycerol and microalgal biomass showed that using the two substrates together had an antagonistic effect on biohydrogen production but a synergistic effect on methane fermentation. This two-stage process provided a more efficient solution compared to a one-stage process, with a total energy of 1.27 kJ/g-VSadded.
FERMENTATION-BASEL
(2021)