Article
Biochemical Research Methods
Bo Yang, Ping Wang, Zhenqing Li, Chunxian Tao, Qingxiang You, Shinichi Sekine, Songlin Zhuang, Dawei Zhang, Yoshinori Yamaguchi
Summary: The concept of time to place conversion in microfluidics allows for faster amplification of target genes, but may result in low throughput amplicons. To address this issue, a microfluidic system based on a CF-PCR array chip was developed to simultaneously amplify multiple target genes in a more efficient manner. This work provides insights into the development of high throughput CF-PCR microfluidic systems for point-of-care testing for simultaneous detection of various pathogens.
Article
Food Science & Technology
Yuan-qing Hu, Wan-ying Wang, Turmidzi Fath, Feng-xia Li, Lin-fang Fang, Zan-hu Zhou, Dan-feng Zhang
Summary: In this study, a PMA-mPCR assay was developed for accurately screening viable vibrios in aquatic foods. The assay exhibited 100% specificity and maintained its sensitivity after PMA treatment. The results showed that the assay could detect viable V. parahaemolyticus, V. alginolyticus, and V. cholerae as low as 1.0 x 102 CFU/mL after 10 hours of enrichment. The practical application of the assay using 453 actual samples also demonstrated its accuracy in screening target vibrios in seafood.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Immunology
Wei-Hsuan Lin, Han-Cheng Chiu, Kuan-Fu Chen, Kuo-Chien Tsao, Yi-Yin Chen, Ting-Hsuan Li, Yhu-Chering Huang, Yu-Chia Hsieh
Summary: In adult patients with CAP, bacterial pathogens accounted for 50% and viruses for 36.3%. Molecular testing could detect more pathogens than traditional culture techniques in diagnosing pneumonia. Co-infections with multiple pathogens, especially K. pneumoniae and human rhinovirus, were common in this study.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Microbiology
Maria Daniela Silva, Antonio Lima, Nuno Marcal, Luis Dias, Miguel Gama, Sanna Sillankorva
Summary: Understanding the bacterial causes of otitis media is crucial for effective treatment. This study analyzed samples from 49 children with otitis media in Portugal, finding a correlation between bacteria in the middle ear fluid and nasopharynx. The most commonly identified species were Moraxella catarrhalis and Streptococcus pneumoniae, but Haemophilus influenzae was more prevalent in the middle ear fluid. The study also revealed the importance of polymicrobial infections in otitis media.
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Murari Bhandari, Irani U. Rathnayake, Lawrence Ariotti, Brett Heron, Flavia Huygens, Mitchelle Sullivan, Amy V. Jennison
Summary: Cholera is a significant public health problem in developing and underdeveloped countries, and it is still of concern in developed countries like Australia due to international travel-related or locally acquired cases. This study focused on monitoring Vibrio cholerae strains in environmental waterways in Queensland, Australia. Through PCR, MALDI-TOF, and whole-genome sequencing, toxigenic and non-toxigenic strains were detected at all sampled sites. Although no epidemic strains were found, continuous surveillance is still necessary to identify potential sources of outbreaks and implement control measures.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Immunology
Joy Chukwu-Osazuwa, Trung Cao, Ignacio Vasquez, Hajarooba Gnanagobal, Ahmed Hossain, Vimbai Irene Machimbirike, Javier Santander
Summary: This study identified common antigens of Gram-negative bacterial pathogens affecting marine finfish aquaculture using reverse vaccinology, providing a basis for the development of universal or polyvalent vaccines.
Article
Chemistry, Analytical
Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma
Summary: BNNPs have been proven to significantly improve the specificity and sensitivity of PCR and LAMP amplification reactions, expanding the application range of nucleic acid detection and increasing detection sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Review
Immunology
Ganeshamoorthy Hariharan, Kandeeparoopan Prasannath
Summary: Phytopathogenic fungal species can cause significant losses in crop yield and quality, making precise and rapid detection and identification of plant infecting fungi essential for effective disease management. DNA-based methods have become popular for accurate plant disease diagnostics, but more reliable, faster, and easier molecular diagnostic techniques are needed for practical use.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Zhenqing Li, Jiahui Liu, Ping Wang, Chunxian Tao, Lulu Zheng, Shinichi Sekine, Songlin Zhuang, Dawei Zhang, Yoshinori Yamaguchi
Summary: This study explored multi-PCR of P.g, T.d, and T.f using a CF-PCR microfluidic chip for the first time, identifying two optimal primer combinations and demonstrating the significant reduction in amplification time with multi-PCR.
Article
Infectious Diseases
Yu Kyung Kim, Jong Ho Lee, Sae Yoon Kim, Ji Young Ahn, Kwang Hae Choi, Young Hwan Lee, Kyung Mi Jang, Yong Sauk Hau, Jae Min Lee
Summary: Multiplex polymerase chain reaction (mPCR) is increasingly used for diagnosing respiratory pathogens in pediatric inpatient facilities, with the adapted FilmArray Respiratory Panel (FA-RP) offering faster and more accurate detection of multiple viruses, leading to reduced antibiotic use and hospital stays in pediatric patients.
Article
Chemistry, Multidisciplinary
Sudheendran Mavila, Heidi R. Culver, Alex J. Anderson, Tania R. Prieto, Christopher N. Bowman
Summary: An athermal approach using self-immolative thioester linked nucleic acids (TENA) for mRNA enrichment from total RNA has shown comparable performance to a commercial kit. The method is compatible with downstream applications such as RT-qPCR for gene expression analysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Biochemistry & Molecular Biology
Li Liu, Tharmala Tharmalingam, Eva Maischberger, Simone Albrecht, Mary E. Gallagher, Raul Miranda-CasoLuengo, Wim G. Meijer, Pauline M. Rudd, Jane A. Irwin
GLYCOCONJUGATE JOURNAL
(2013)
Article
Agriculture, Dairy & Animal Science
K. Pluta, J. A. Irwin, C. Dolphin, L. Richardson, E. Fitzpatrick, M. E. Gallagher, C. J. Reid, M. A. Crowe, J. F. Roche, P. Lonergan, S. D. Carrington, A. C. O. Evans
JOURNAL OF ANIMAL SCIENCE
(2011)
Article
Education, Scientific Disciplines
Marion T. Ryan, Alan W. Baird, Clive W. Mulholland, Jane A. Irwin
JOURNAL OF VETERINARY MEDICAL EDUCATION
(2009)
Article
Cell Biology
Anna D. Sobkowicz, Mary E. Gallagher, Colm J. Reid, Daniel Crean, Stephen D. Carrington, Jane A. Irwin
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Daniela Quaglia, Jane A. Irwin, Francesca Paradisi
MOLECULAR BIOTECHNOLOGY
(2012)
Article
Cell Biology
Katarzyna Pluta, Paul A. McGettigan, Colm J. Reid, John A. Browne, Jane A. Irwin, Tharmala Tharmalingam, Anthony Corfield, Alan Baird, Brendan J. Loftus, Alexander C. O. Evans, Stephen D. Carrington
PHYSIOLOGICAL GENOMICS
(2012)
Article
Veterinary Sciences
Colm J. Reid, Laura Thompson, Samantha Donnelly, Christopher M. Deaton, David J. Marlin, Stephen D. Carrington, Jane A. Irwin
RESEARCH IN VETERINARY SCIENCE
(2009)
Article
Veterinary Sciences
Karol Fijalkowski, Danuta Czernomysy-Furowicz, Jane A. Irwin, Pawel Nawrotek, Anna Pobucewicz
RESEARCH IN VETERINARY SCIENCE
(2012)
Article
Veterinary Sciences
Eva Maischberger, Carolyn A. Cummins, Eamonn Fitzpatrick, Mary E. Gallagher, Sheila Worrall, Karine Rousseau, David J. Thornton, Wim G. Meijer, Raul Miranda-CasoLuengo, Vivienne E. Duggan, Stephen D. Carrington, Jane A. Irwin, Colm J. Reid
RESEARCH IN VETERINARY SCIENCE
(2013)
Article
Food Science & Technology
Paul E. W. Morrissey, Michael A. Folan, Karol Fijalkowski, Alan W. Baird, Jane A. Irwin
JOURNAL OF FUNCTIONAL FOODS
(2012)
Article
Biochemistry & Molecular Biology
Emma L. Kavanagh, Melinda Halasz, Paul Dowling, Jo Withers, Sinead Lindsay, Michaela J. Higgins, Jane A. Irwin, Pauline M. Rudd, Radka Saldova, Amanda McCann
Summary: Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options. Chemotherapy-induced senescent cells release more extracellular vesicles (EVs) and show significant differences in glycan profiles compared to non-senescent cells. This study suggests potential for using N-glycan moieties for monitoring treatment response and targeting senescent cancer cells.
Article
Veterinary Sciences
E. Maischberger, J. A. Irwin, S. D. Carrington, V. E. Duggan
IRISH VETERINARY JOURNAL
(2008)
Article
Immunology
R. J. Flynn, J. A. Irwin, M. Olivier, M. Sekiya, J. P. Dalton, G. Mulcah
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2007)
Article
Biochemistry & Molecular Biology
Mary Sekiya, Grace Mulcahy, Jane A. Irwin, Colin M. Stack, Sheila M. Donnelly, Weibo Xu, Peter Collins, John P. Dalton