Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 20, Issue 7, Pages 4551-4561Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-012-1354-6
Keywords
Antioxidant response; Ceylon spinach; Comet assay; Pb stress; Plant bioassay; Tissue ultrastructure
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Funding
- University of Hyderabad through the University Grant Commission, New Delhi
- Ministry of Environment and Forests, GOI, New Delhi (MoEF) [17/3/2010-RE]
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Hydroponic experiments were performed with Talinum triangulare (Jacq.) Willd. focusing the root cellular biochemistry with special emphasis on DNA damage, structural, and elemental analyses in Pb(NO3)(2) exposed with 0, 0.25, 0.5, 0.75, 1.0, and 1.25 mM for 7 days. Lead (Pb) increased reactive oxygen species production, lipid peroxidation, protein oxidation, cell death, and DNA damage and decreased the protein content in a dose-dependent manner. Likewise, a dose-dependent induction of antioxidative enzymes superoxide dismutase and catalase by Pb was evident. Ascorbate peroxidase on the other hand responded biphasically to Pb treatments by showing induction at low (0.25 and 0.50) and repression at high (0.75-1.25 mM) concentrations. The estimation of proline content also indicated a similar biphasic trend. Scanning electron microscope and energy-dispersive X-ray spectroscopy analysis showed that 1.25 mM Pb treatment resulted in ultrastructural modifications in roots and stem tissue that was marked by the change in the elemental profile. The findings pointed to the role of oxidative stress in the underlying Pb phytotoxicity and genotoxicity in T. triangulare.
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