Review
Biotechnology & Applied Microbiology
German A. Islan, Boris Rodenak-Kladniew, Nehuen Noacco, Nelson Duran, Guillermo R. Castro
Summary: This review summarizes the general aspects of prodigiosin (PG), a bio-dye with a wide spectrum of biological activities. The synthesis, production process, and potential delivery systems for improving its bioavailability are discussed. Additionally, novel applications to enhance its biological activity in biomedical applications are proposed.
Article
Chemistry, Applied
Arivalagan Pugazhendhi, Thangavel Mathimani, Sulaiman Ali Alharbi, Arunachalam Chinnathambi, Indira Karuppusamy, Kathirvel Brindhadevi, Kantaporn Kheawfu, Surachai Pikulkaew
Summary: ZnO NPs exhibit strong antibacterial activity through interaction with bacterial cells, and ZnO NPs synthesized using plant extract are safer and non-toxic, serving as a bactericidal solution against pathogenic bacteria.
PROGRESS IN ORGANIC COATINGS
(2022)
Review
Biochemistry & Molecular Biology
Rafael G. Araujo, Natalia Rodriguez Zavala, Carlos Castillo-Zacarias, Mario E. Barocio, Enrique Hidalgo-Vazquez, Lizeth Parra-Arroyo, Jesus Alfredo Rodriguez-Hernandez, Maria Adriana Martinez-Prado, Juan Eduardo Sosa-Hernandez, Manuel Martinez-Ruiz, Wei Ning Chen, Damia Barcelo, Hafiz M. N. Iqbal, Roberto Parra-Saldivar
Summary: Bionanocomposites based on natural bioactive entities, formulated with biological molecules with antimicrobial, antioxidant, and anticancer activities, are of great importance. This review focuses on the pyrrolic compound produced by microorganisms, particularly Serratia marcescens, and its potential as a carrier for controlled release of prodigiosin, a bioactive secondary metabolite. The review also highlights recent developments in pyrrole-based bionanocomposites and their application in the medical field.
Article
Biotechnology & Applied Microbiology
Xuewei Pan, Mi Tang, Jiajia You, Fei Liu, Changhao Sun, Tolbert Osire, Weilai Fu, Ganfeng Yi, Taowei Yang, Shang-Tian Yang, Zhiming Rao
Summary: This study identified that the transcriptional regulator RcsB negatively controlled prodigiosin biosynthesis in S. marcescens. Additionally, RcsB was found to regulate other important cellular processes, providing insight into its function in cell motility, capsular polysaccharide production, and acid resistance in S. marcescens.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jaganathan Anitha, Aathi Muthusankar, Sundram Viswanathan, Palanivel Murugesan, Nallani Chakravarthula Gayathri, Rajendran Selvakumar, Thathan Premkumar
Summary: In this study, prodigiosin produced by Serratia marcescens (SM) was isolated and optimized from different natural sources. The isolated prodigiosin showed good antimicrobial activity and higher binding efficiency for COX-2 gene. Furthermore, it was found to be non-toxic to rat islet cells and exhibited anti-inflammatory properties.
PROCESS BIOCHEMISTRY
(2023)
Article
Microbiology
Junjie Lin, Yanshuang Yu, Ke Zhao, Jie Zhao, Christopher Rensing, Jichen Chen, Xianbo Jia
Summary: This study reveals that ptrA gene regulates the synthesis of prodigiosin in S. marcescens, as well as important biological functions including motility, biofilm formation, hemolytic activity, and stress tolerance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Xianbo Jia, Ke Zhao, Fangchen Liu, Junjie Lin, Chenqiang Lin, Jichen Chen
Summary: This study reveals the regulatory mechanism of prodigiosin production by the transcription factor OmpR. OmpR positively regulates prodigiosin production by directly binding to the promoter region of the prodigiosin biosynthesis cluster. Additionally, OmpR also regulates its own expression, further affecting prodigiosin production.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Xianbo Jia, Fangchen Liu, Ke Zhao, Junjie Lin, Yu Fang, Shouping Cai, Chenqiang Lin, Hui Zhang, Longjun Chen, Jichen Chen
Summary: Prodigiosin, a promising secondary metabolite produced mainly by Serratia strains, was studied through mutagenesis library construction with EZ-Tn5 transposon, revealing 33 genes involved in prodigiosin production can be classified into transcription-regulating, membrane protein-encoding, and metabolism enzyme-encoding genes. The downregulation of pigA gene transcription in mutants with different inserted genes corresponded to decreased prodigiosin production, while functional confirmation of mutant genes in the pyrimidine nucleotide biosynthesis pathway was conducted by adding orotate and uridylate to the medium. Gene complementation confirmed the regulatory role of EnvZ/OmpR two-component regulatory system genes envZ and ompR in prodigiosin production.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wei Chen, Ruyi Chen, Jianyun Cao
Summary: A genome-editing strategy based on Red recombineering was established for S. marcescens, allowing efficient genetic modification and potential mass production of useful metabolites. Multiple types of modification and recombination strategies can be easily performed in this species.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xue Li, Xinfeng Tan, Qingshuang Chen, Xiaoling Zhu, Jing Zhang, Jie Zhang, Baolei Jia
Summary: Oral administration of prodigiosin can improve the intestinal microbiota of mice, demonstrating potential probiotic effects.
Article
Energy & Fuels
Nisarg Gohil, Gargi Bhattacharjee, Ravi Kalariya, Vikrant Pandya, Khushal Khambhati, Jigresh Gohil, Khalid J. Alzahrani, Pau-Loke Show, Rupesh Maurya, Vijai Singh
Summary: The study successfully enhanced prodigiosin production by using soybean meal as the sole nutrition source and optimizing the addition of sucrose and glycine. This approach provides a cost-effective and environmentally friendly method to promote bioproduction of prodigiosin, supporting the circular economy.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Microbiology
L. Meng-xi, Huang Hui-bin, Long Jie-yun, C. A. O. Jing-xiao, Zhang Zhen-wang
Summary: After isolation and screening, an antibiotic-resistant bacterial isolate, named S. spectabilis strain L20190601, was identified. The red pigment produced by this strain was characterized, showing strong antibacterial activity against eight clinically common pathogens. These findings suggest that metacycloprodigiosin has potential as a new type of broad-spectrum antibacterial drug.
CURRENT MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Junqing Wang, Tingting Zhang, Yang Liu, Shanshan Wang, Zerun Li, Ping Sun, Hui Xu
Summary: This study found that yeast extract affects the synthesis of prodigiosin in Serratia marcescens through multiple pathways. It downregulates the expression of key enzymes in the glycolysis pathway and upregulates genes related to other carbon metabolism pathways. Additionally, the presence of metal ions and the accumulation of fermentation metabolites alter the two-component signaling system and regulate metabolism to different extents. These findings shed light on the mechanism of prodigiosin regulatory inhibition and provide new insights into the metabolic control of S. marcescens.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Microbiology
Xuezheng Zhao, Dongqing Xu, Wenxiao Xia, Menghua Hu, Xuede Peng, Xia Liu, Tingting Ran, Weiwu Wang
Summary: This study constructed a transposon mutant library in S. marcescens FS14 and identified genes involved in the regulation of prodigiosin biosynthesis. The transposon insertion led to overexpression of RpoH, which resulted in the inhibition of prodigiosin production. Increasing the expression of RpoS alleviated the inhibitory effect of RpoH on prodigiosin production. Manipulating the transcription levels of sigma factors could be applied to regulate the production of secondary metabolites.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pryce L. Haddix
Summary: This study expanded the functional analysis of prodigiosin pigment to high-density and stationary phases using chemostat culture, showing a positive correlation between prodigiosin levels and ATP levels in high-density growth, and a decline in ATP levels during late stationary phase despite high prodigiosin levels. This provides evidence for the various effects of prodigiosin on ATP production throughout the growth cycle.
CANADIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Plant Sciences
Faiza Baali, Sabah Boumerfeg, Amel Boudjelal, Marcella Denaro, Giovanna Ginestra, Abderrahmane Baghiani, Nadjat Righi, Amirouche Deghima, Faycel Benbacha, Antonella Smeriglio, Domenico Trombetta
Summary: The aim of this study was to scientifically validate the traditional use of Algerian Lavandula stoechas and Mentha pulegium. The methanol extracts of both plants showed strong antioxidant activity and exhibited antimicrobial activity against S. aureus. Additionally, ointments containing these extracts accelerated the wound healing process.
Article
Biochemistry & Molecular Biology
Lucia Caputo, Laura Cornara, Francesco Maria Raimondo, Vincenzo De Feo, Stefano Vanin, Marcella Denaro, Domenico Trombetta, Antonella Smeriglio
Summary: The study investigated the micromorphology of Mentha pulegium leaves and flowers harvested in three different Sicilian areas, as well as the phytochemical profile, phytotoxic activity, and eco-compatibility of their essential oils. The results showed that oxygenated monoterpenes were the most abundant class in all the essential oils, and soil salinity strongly affected the expression of the toxic metabolite pulegone. Phytotoxicity tests demonstrated a moderate activity of the essential oils against selected species, suggesting the potential use of the oils as eco-friendly herbicides.
Review
Food Science & Technology
Davide Barreca, Domenico Trombetta, Antonella Smeriglio, Giuseppina Mandalari, Orazio Romeo, Maria Rosa Felice, Giuseppe Gattuso, Seyed Mohammad Nabavi
Summary: Flavonols are bioactive compounds with health-promoting properties, including antioxidation, cardioprotection, antibacterial, antiviral, and anticancer activities. Incorporating flavonols into the human diet is highly recommended for their impressive health benefits, making them excellent nutraceuticals and ingredients for functional food. The metabolization process of flavonols also produces compounds with significant bioactivities, similar to their precursors.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Review
Cell Biology
Javad Sharifi-Rad, Cristina Quispe, Muhammad Imran, Abdur Rauf, Muhammad Nadeem, Tanweer Aslam Gondal, Bashir Ahmad, Muhammad Atif, Mohammad S. Mubarak, Oksana Sytar, Oxana Mihailovna Zhilina, Ekaterina Robertovna Garsiya, Antonella Smeriglio, Domenico Trombetta, Daniel Gabriel Pons, Miquel Martorell, Susana M. Cardoso, Ahmad Faizal Abdull Razis, Usman Sunusi, Ramla Muhammad Kamal, Lia Sanda Rotariu, Monica Butnariu, Anca Oana Docea, Daniela Calina
Summary: Genistein, an isoflavone found in plants, exhibits various biological activities such as antioxidant, anti-inflammatory, antibacterial, and antiviral effects. It is known to protect against cardiovascular diseases and reduce the incidence of certain cancers, especially breast cancer, by displacing estrogen from cellular receptors and blocking hormonal activity. Further research is needed to explore the efficacy, safety, and potential of nanotechnologies to enhance bioavailability and therapeutic efficacy of genistein.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Fisheries
Vincenzo Zammuto, Maria Giovanna Rizzo, Antonio Spano, Giuseppa Genovese, Marina Morabito, Damiano Spagnuolo, Fabiano Capparucci, Claudio Gervasi, Antonella Smeriglio, Domenico Trombetta, Salvatore Guglielmino, Marco Sebastiano Nicolo, Concetta Gugliandolo
Summary: The extraction of polysaccharides from algae can inhibit bacterial adhesion, thus preventing biofilm formation. Algal extracts show significant inhibitory activity against biofilm formation and have potential for environmentally friendly applications in aquaculture.
Article
Plant Sciences
Laura Cornara, Gabriele Ambu, Alex Alberto, Domenico Trombetta, Antonella Smeriglio
Summary: This study investigated the micromorphological and phytochemical features, as well as the antioxidant and anti-inflammatory properties of leaves from several plant species used in the salad of the Capuchin monks. The extracts obtained from blanched leaves showed the highest content of bioactive compounds and exhibited the most marked antioxidant and anti-inflammatory activity.
Article
Chemistry, Applied
Antonella Smeriglio, Marcella Denaro, Emilio Di Gristina, Luca Mastracci, Federica Grillo, Laura Cornara, Domenico Trombetta
Summary: This study found that Citrus lumia seeds contain abundant bioactive compounds that exhibit good antioxidant and anti-inflammatory activities. In cell and human primary cell experiments, methanol extract of the seeds showed the strongest inhibitory and protective effects.
Article
Biochemistry & Molecular Biology
Cristina Occhiuto, Gianluigi Aliberto, Mariarosaria Ingegneri, Domenico Trombetta, Clara Circosta, Antonella Smeriglio
Summary: This study evaluated the nutritional and phytochemical characteristics of cold-pressed hempseed oil and hempseed meal from two industrial hemp varieties. The oil exhibited high levels of phenolic compounds, vitamin E, and a well-balanced ratio of fatty acids, with strong antioxidant activity. The hempseed meal was rich in protein, fiber, and amino acids, with low levels of anti-nutritional factors, suggesting potential use in nutraceutical products.
Review
Medicine, General & Internal
Susanna Campo, Antonio Lacquaniti, Domenico Trombetta, Antonella Smeriglio, Paolo Monardo
Summary: This review focuses on the pathways related to immune system dysfunction and inflammation in hemodialysis patients. Thrombogenicity and oxidative stress are considered important characteristics of biocompatibility in hemodialysis. Immune dysfunction and inflammation are closely linked with uremic toxins in hemodialysis patients, affecting clinical outcomes and mortality.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Nutrition & Dietetics
Nadia Panera, Maria Rita Braghini, Annalisa Crudele, Antonella Smeriglio, Marzia Bianchi, Angelo Giuseppe Condorelli, Rebecca Nobili, Libenzio Adrian Conti, Cristiano De Stefanis, Gessica Lioci, Fabio Gurrado, Donatella Comparcola, Antonella Mosca, Maria Rita Sartorelli, Vittorio Scoppola, Gianluca Svegliati-Baroni, Domenico Trombetta, Anna Alisi
Summary: This study investigated the potential anti-fibrotic effect of HXT + VitE combination therapy on NAFLD-related fibrosis. The results demonstrated that HXT + VitE treatment reduced proliferation, migration, and contraction of fibrosis cells, decreased expression of pro-fibrogenic genes, and antagonized the pro-fibrotic effects by interfering with transcription factor translocation/activation. In mouse and pediatric NAFLD models, HXT + VitE treatment showed a reduction in fibrosis.
Article
Food Science & Technology
Paola Malaspina, Federica Betuzzi, Mariarosaria Ingegneri, Antonella Smeriglio, Laura Cornara, Domenico Trombetta
Summary: This study conducted an in-depth evaluation of laurel and cherry laurel leaves to distinguish their distinguishing features. This multidisciplinary approach can be useful in plant identification, quality control tests, intoxications, and criminal cases.
Article
Genetics & Heredity
Paola Gualtieri, Marco Marchetti, Giulia Frank, Antonella Smeriglio, Domenico Trombetta, Carmela Colica, Rossella Cianci, Antonino De Lorenzo, Laura Di Renzo
Summary: The Mediterranean Diet is associated with beneficial effects against chronic non-communicable diseases. A study on 24 subjects showed that 2-week supplementation with mixed apple and bergamot juice had a positive impact on body composition, biochemical profile, and oxidative and inflammatory gene expression. The supplementation resulted in increased lean mass, reduced total cholesterol/HDL index, and improved gene expression related to CNCDs.
Article
Nutrition & Dietetics
Paola Gualtieri, Domenico Trombetta, Antonella Smeriglio, Giulia Frank, Angela Alibrandi, Giulia Leggeri, Marco Marchetti, Ilaria Zingale, Silvia Fanelli, Arianna Stocchi, Laura Di Renzo
Summary: Supplementation has a positive impact on vaccination response, enhancing immune defenses and reducing side effects. Our study analyzed the relationship between vaccine side effects and supplement intake among the Italian population. The results showed a significant correlation between supplement consumption and side effects at the end of the vaccination cycle, as well as associations with specific side effects such as diarrhea and nausea.
Article
Nutrition & Dietetics
Mariarosaria Ingegneri, Antonella Smeriglio, Rossana Rando, Teresa Gervasi, Maria Pia Tamburello, Giovanna Ginestra, Erminia La Camera, Rosamaria Pennisi, Maria Teresa Sciortino, Giuseppina Mandalari, Domenico Trombetta
Summary: This study investigated the nutritional and polyphenolic profile, as well as the antioxidant, antimicrobial, antiviral, and potential prebiotic effects of blanched almond skin (BS) and blanch water (BW) from three different Sicilian cultivars. The results showed that the total phenols and flavonoids contents were high in BS and BW. BS exhibited antiviral activity against herpes simplex virus 1. It also had high fiber and protein contents and low fat and sugar contents, making it nutritionally valuable. The study indicated that cultivar does not significantly affect the chemical and biological properties of BS and BW.
Article
Pharmacology & Pharmacy
Laura Di Renzo, Antonella Smeriglio, Mariarosaria Ingegneri, Paola Gualtieri, Domenico Trombetta
Summary: Current evidence supports the use of EVOO and its minor components to improve cardiovascular and metabolic health. This study investigated the pharmacokinetics of DOPET, a bioactive compound in EVOO, on 20 healthy volunteers. Results showed higher bioavailability of DOPET compared to literature, suggesting the role of pharmaceutical formulation in its bioavailability and pharmacokinetics.
