Uptake and elimination, and effect of estrogen-like contaminants in estuarine copepods: an experimental study

In recent years, anthropogenic chemicals which can disrupt the hormonal systems of both humans and wildlife have been raised to a major cause of concern. The aim of the present work was to determine the bioconcentration factors of the two major alkylphenols (AP) of the Seine Estuary [4-nonylphenol (4 NP) and nonylphenol acetic acid (NP1EC)] and of the synthetic estrogen, estrogen ethinylestradiol (EE2), in Eurytemora affinis after exposure in a continuous flow-through system under environmental realistic conditions. Moreover, the elimination of these compounds in copepods from the Seine Estuary has been investigated by measuring concentrations after 1 week in clean water in comparison to background levels. In this study, the dominant copepod species of the Seine Estuary, E. affinis, was exposed at environmental relevant concentrations under laboratory-controlled conditions to selected waterborn contaminants, a mixture of 4 NP/NP1EC, and a synthetic EE2. The uptake and the elimination of these contaminants by E. affinis have been studied. The results show that, at the end of the uptake period, both 4 NP and NP1EC, and also EE2 were accumulated in exposed copepods with respective concentration factors of 324, 3,020, and 5,383. A rapid elimination of these compounds was also observed in copepods placed in clean water since 54% of total NP1EC and 100% of EE2 amounts have been lost after 3 days. Pregnenolone was synthesized after exposure to EE2 and AP mixture. These results demonstrate that E. affinis has the potency to accumulate but also to eliminate endocrine disrupters which suggests a non-negligible role of this copepod species in the biogeochemical cycles of these contaminants in estuarine ecosystems. Hence, these results also suggest that a transfer of 4 NP, NP1EC, and EE2 to copepod predators and subsequently that secondary poisoning of these organisms might be possible. Estrogen-like contaminants can induce pregnenolone synthesis and affect the reproduction of E. affinis. These results suggest the important role of this copepod species in biogeochemical cycles of non-ionic surfactants as well as synthetic steroids in estuarine ecosystems. E. affinis could be a non-negligible route of exposure for juvenile fish and underline the potential for deleterious effects on copepod predators.