Article
Endocrinology & Metabolism
Wiwat Rodprasert, Jaakko J. Koskenniemi, Helena E. Virtanen, Sergey Sadov, Antti Perheentupa, Helena Ollila, Jakob Albrethsen, Anna Maria Andersson, Anders Juul, Niels E. Skakkebaek, Katharina M. Main, Jorma Toppari
Summary: There are differences in pubertal hormone levels between boys with a history of cryptorchidism who experienced spontaneous testicular descent or underwent orchiopexy and boys without a history of cryptorchidism.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Shih-Min Hsia, Yi-Fen Chiang, Hsin-Yuan Chen, Mohamed Ali, Paulus S. Wang, Kai-Lee Wang
Summary: The study found that a high-fructose diet can lead to decreased plasma testosterone levels in rats, affecting the responsiveness of Leydig cells to hormones and the activity of the hypothalamus-pituitary-gonadal (HPG) axis.
Article
Biochemistry & Molecular Biology
Sha Han, Jiaqiang Luo, Shuai Xu, Liangyu Zhao, Chencheng Yao, Junwei Xu, Ruhui Tian, Erlei Zhi, Yuhua Huang, Shujie Xia, Zheng Li, Peng Li
Summary: Aging negatively affects human testicular function, leading to impaired steroidogenesis and decreased testosterone levels. Low-intensity pulsed ultrasound (LIPUS) has been shown to increase testosterone levels in animal models and is a non-invasive treatment option for male erectile dysfunction. This study investigates the effect of LIPUS on human senescent Leydig cells (LCs) and finds that stimulation with LIPUS improves the senescence phenotype and enhances LCs' secretory function, increasing the expression of key pathways in testosterone synthesis. These findings suggest that LIPUS may be a novel treatment for human senescent LCs with decreased testosterone secretion levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Huitao Li, Zina Wen, Chaobo Ni, Xianwu Chen, Yong Cheng, Yuxin Liu, Xiaoheng Li, Qiqi Zhu, Ren-Shan Ge
Summary: Short-term exposure to PFDoA in adulthood delayed Leydig cell regeneration by preventing Leydig cells from stem cells through multiple mechanisms.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Review
Endocrinology & Metabolism
Indrashis Bhattacharya, Souvik Dey
Summary: Leydig cells (Lc) in the testis play a critical role in male fertility by producing testosterone in response to luteinizing hormone (LH). Recent studies have identified the diverse origins of these cells and their differential transcriptomic and steroidogenic outputs. Fetal Lc (FLc) originate from coelomic epithelium and perivascular cells, and gradually give way to adult Lc (ALc), which are considered to be derived from FLc and perivascular cells. This article summarizes the current understanding of Lc development, their cellular origins, and their functional significance in rodent and primate testes.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Environmental Sciences
Xinrui Hao, Xiaoju Guan, Xingxing Zhao, Minpeng Ji, Xin Wen, Panpan Chen, Fenfen Chen, Jianying Yang, Qingquan Lian, Leping Ye, Haolin Chen
Summary: Studies have shown that phthalates, such as MEHP, can significantly inhibit the proliferation and differentiation of stem Leydig cells (SLCs). It also affects the differentiation of SLCs into Leydig cells and adipocytes.
Article
Endocrinology & Metabolism
Dandan Luo, Xiangyu Qi, Xiaoqin Xu, Leilei Yang, Chunxiao Yu, Qingbo Guan
Summary: Age-related decline in testosterone is associated with Leydig cell aging, and obesity accelerates this process. The study found that p38 MAPK is involved in Leydig cell aging induced by age and obesity. Inhibition of p38 MAPK can delay Leydig cell aging and alleviate the decline in testosterone.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Environmental Sciences
Shijun Li, Yun Wang, Cheng Zou, Qiqi Zhu, Yiyan Wang, Haiqiong Chen, Wenjing Yang, Yuhan Tu, Haoni Yan, Xiaoheng Li, Ren-Shan Ge
Summary: This study investigated the effects of cypermethrin exposure on the development and function of Leydig cells in male rats during late puberty. The results showed that cypermethrin reduced testosterone levels and increased luteinizing hormone levels, while decreasing the number of Leydig cells. Furthermore, cypermethrin induced oxidative stress and mitochondrial damage.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jinlian Liang, Hanhao Li, Jiaxin Mei, Zhen Cao, Yan Tang, Rufei Huang, Huan Xia, Qihao Zhang, Qi Xiang, Yan Yang, Yadong Huang
Summary: The study revealed that SC-derived miR-145-5p plays a significant role in regulating the functions of Leydig cells and may serve as a diagnostic biomarker for male hypogonadism developmental abnormalities during puberty.
Article
Environmental Sciences
Lipeng Li, Zijie Pei, Ruiting Wu, Yaling Zhang, Yaxian Pang, Huaifang Hu, Wentao Hu, Zihan Geng, Tengfei Feng, Yujie Niu, Guimin Hao, Rong Zhang
Summary: Epidemiological studies have revealed an association between chronic exposure to PM2.5 and male infertility, but the underlying mechanisms were unclear. In this study, mice models exposed to PM2.5 showed decreased sperm quality and increased testosterone levels. Ferrostatin-1 treatment effectively mitigated PM2.5-induced testicular dysfunction. Further investigations showed that PM2.5 exposure led to ferroptosis in Leydig cells, which was partially rescued by Fer-1 intervention. The upregulation of FDX1, a mediator involved in steroidogenesis, caused cellular ROS elevation and ferrous iron overload, leading to ferroptotic cell death and increased testosterone levels.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Reproductive Biology
Banu Orta Yilmaz, Yasemin Aydin
Summary: Arsenite and fluoride, commonly found in underground drinking water, have negative impacts on testicular function and fertility. In mouse Leydig cells, exposure to individual or combined arsenite and fluoride induces oxidative stress, cell apoptosis, alters antioxidant balance, and suppresses steroidogenic enzyme expression. This study highlights the detrimental effects of environmentally relevant concentrations of arsenite and fluoride on testosterone production in Leydig cells and male infertility.
Article
Environmental Sciences
Yunbing Tang, Yingfen Ying, Cheng Zou, Haoni Yan, Yiyan Wang, Huitao Li, Xiaoheng Li, Zhangye Xu, Jieqiang Lv, Ren-shan Ge
Summary: PFTeDA negatively impacts Leydig cell function by inducing oxidative stress and cell apoptosis. This results in decreased testosterone levels, increased luteinizing hormone levels, altered sperm number, and changes in cell biomarkers.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Endocrinology & Metabolism
Jingjing Shao, Jiexia Wang, Xin Wen, Jiajia Xie, Fu Huang, Xiaoju Guan, Xinrui Hao, Ping Duan, Congde Chen, Haolin Chen
Summary: Aging and testicular macrophages may affect the function of stem Leydig cells, leading to a decrease in their numbers and a reduction in their differentiation ability.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Sudeep Kumar, Hyo Jeong Kim, Chul-Ho Lee, Hueng-Sik Choi, Keesook Lee
Summary: Testosterone is essential for the development and function of the male reproductive system. DAX1-deficient mice exhibit higher levels of steroids and increased expression of steroidogenic genes in the testes, suggesting a role for DAX1 in regulating steroidogenesis. Furthermore, these mice seem to reach puberty earlier, highlighting the impact of DAX1 on testis development.
REPRODUCTIVE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Alexander Siegfried Busch, Marie Lindhardt Ljubicic, Emmie N. Upners, Margit Bistrup Fischer, Lars Lau Raket, Hanne Frederiksen, Jakob Albrethsen, Trine Holm Johannsen, Casper P. Hagen, Anders Juul
Summary: The male hypothalamic-pituitary-gonadal axis is transiently active during the first months of life, with surging serum concentrations of reproductive hormones, a period known as minipuberty. In healthy male infants, reproductive hormone concentrations show marked dynamics during the first 6 months, with correlations with testicular volume and establishment of reference curves for serum levels of reproductive hormones.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Environmental Sciences
Ahmed S. Mandour, Haney Samir, Marwa A. El-Beltagy, Mohamed M. Abdel-Daim, Watanabe Izumi, Danfu Ma, Katsuhiro Matsuura, Ryou Tanaka, Gen Watanabe
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Reproductive Biology
Haney Samir, Paul Nyametease, Mohamed Elbadawy, Kentaro Nagaoka, Kazuaki Sasaki, Gen Watanabe
Article
Reproductive Biology
Haney Samir, Mohamed A. I. El Sayed, Kentaro Nagaoka, Kazuaki Sasaki, Amal M. Abo El-Maaty, Aly Karen, Mostafa M. Abou-Ahmed, Gen Watanabe
Article
Agriculture, Dairy & Animal Science
Shaoxia Pu, Kento Usuda, Kentaro Nagaoka, Andrea Gore, David Crews, Gen Watanabe
Article
Reproductive Biology
Haney Samir, Paul Nyametease, Mohamed Elbadawy, Mohamed Fathi, Ahmed S. Mandour, Faten Radwan, Kentaro Nagaoka, Kazuaki Sasaki, Gen Watanabe
Article
Environmental Sciences
Ahmed S. Mandour, Roushdy F. Elsayed, Asmaa O. Ali, Ahmed E. Mahmoud, Haney Samir, Amina A. Dessouki, Katsuhiro Matsuura, Izumi Watanabe, Kazuaki Sasaki, Salim Al-Rejaie, Tomohiko Yoshida, Kazumi Shimada, Ryou Tanaka, Gen Watanabe
Summary: The study found that copper deficiency leads to significant pathological changes in the hearts of goats, including myocardial injury and cardiac dilatation, as well as affecting various hemato-biochemical parameters. ECG and echocardiography can potentially detect the cardiac changes induced by CuD.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Marine & Freshwater Biology
Shohei Kobayashi, Takuya Fukuoka, Chihiro Kinoshita, Katsufumi Sato, Katsuhiko Arai, Gen Watanabe
Article
Zoology
Shohei Kobayashi, Daisuke Endo, Satomi Kondo, Chiyo Kitayama, Ryuta Ogawa, Katsuhiko Arai, Gen Watanabe, Maiko Kawaguchi
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Watcharee Sovijit, Watcharin Sovijit, Yuriko Ishii, Jun Kambe, Tomoyuki Fujita, Gen Watanabe, Hirohito Yamaguchi, Kentaro Nagaoka
Summary: CPEB1 is an estrogen-responsive gene in breast cancer, its expression is regulated by estrogen activity, promoting breast cancer cell migration. Cell experiments show that estrogen treatment or CPEB1 knockdown leads to enhanced migration capacity in breast cancer cells, indicating that increasing CPEB1 expression level may help control breast cancer behavior.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Reproductive Biology
Haney Samir, Faten Radwan, Gen Watanabe
Summary: The potential applications of Doppler imaging technology in farm animals are mostly limited to females, particularly in assessing testicular blood perfusion. Color Doppler ultrasonography plays a crucial role in evaluating blood flow to the testis in various animal species. Despite the significant studies highlighting the importance of TBP assessment, there are still limitations in routine on-farm application for breeding soundness assessments.
Article
Developmental Biology
Haney Samir, Ahmed S. Mandour, Faten Radwan, Ayman A. Swelum, Tomohiko Yoshida, Ryou Tanaka, Kentaro Nagaoka, Gen Watanabe
Summary: This study examines the effect of diurnal variations on testicular blood flow, testis volume, testicular echogenicity, and reproductive hormones in goats over a 24-hour period. The results indicate that diurnal rhythm induces significant changes in testicular blood flow, testicular echotexture, and circulating reproductive hormones. It is advisable to monitor testicular blood flow at a fixed time each day to avoid the influence of circadian rhythm.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Agriculture, Dairy & Animal Science
Haney Samir, Ahmed S. Mandour, Faten Radwan, Ahmed Ezzat Ahmed, Maha Abdullah Momenah, Nouf Arkan Aldawood, Tomihiko Yoshida, Gen Watanabe, Hossam R. El-Sherbiny
Summary: The study found that exogenous intravenous injection of melatonin can increase testicular artery blood flow and induce changes in most studied hormones. These data are valuable for improving animal productivity under heat-stress environments, as there are close correlations between testicular artery blood flow and potential animal fertility. In addition, peripheral metabolomic analysis revealed important metabolites that can be used for further research on underlying mechanisms.
Article
Medical Informatics
Ayako Maeda-Minami, Tetsuhiro Yoshino, Tetsuro Yumoto, Kayoko Sato, Atsunobu Sagara, Kenjiro Inaba, Hidenori Kominato, Takao Kimura, Tetsuya Takishita, Gen Watanabe, Tomonori Nakamura, Yasunari Mano, Yuko Horiba, Kenji Watanabe, Junzo Kamei
Summary: This study developed a natural language classification system using IBM Watson to provide and judge information about Kampo medicines in Japan. The system classifies questions based on natural language input and outputs preset answers. The system has been validated to give appropriate answers to most questions.
BMC MEDICAL INFORMATICS AND DECISION MAKING
(2023)
Article
Oncology
Daisuke Yamai, Yoshifumi Shimada, Masato Nakano, Hikaru Ozeki, Akio Matsumoto, Kaoru Abe, Yosuke Tajima, Mae Nakano, Hiroshi Ichikawa, Jun Sakata, Takahiro Nagai, Yiwei Ling, Shujiro Okuda, Gen Watanabe, Hitoshi Nogami, Satoshi Maruyama, Yasumasa Takii, Toshifumi Wakai
Summary: This study aimed to clarify the clinical significance of tumor deposits (TDs) in rectal cancer, particularly in the lateral pelvic lymph node (LPLN) area. The number of metastatic foci, including lymph node metastases (LNMs) and TDs, in the LPLN area is useful for risk stratification of patients with low rectal cancer.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Miyu Kojima, Hong Liu, Satoshi Takemoto, Yoshihito Suda, Ryo Inoue, Gen Watanabe, Wanzhu Jin, Kentaro Nagaoka
Summary: The initial colonization and early development of the intestinal microbiome are important for the growth of calves. This study investigated temporal changes in the fecal microbiota, aiming to identify bacteria and blood metabolites associated with calf diarrhea.
FRONTIERS IN ANIMAL SCIENCE
(2022)
