Article
Immunology
Yangyang Li, Weidong Sun, Quan Wang, Ying Yu, Ying Wan, Kai Zhou, Rong Guo, Xiangan Han, Zhaoguo Chen, Weihuan Fang, Wei Jiang
Summary: This study provides evidence that HutC protein in Vibrio parahaemolyticus plays important roles in bacterial stress response, swimming motility, biofilm formation, cytotoxicity, virulence, and gene regulation.
MICROBIAL PATHOGENESIS
(2022)
Article
Plant Sciences
Mingfa Lv, Yufan Chen, Ming Hu, Qinglin Yu, Cheng Duan, Sixuan Ye, Jinfeng Ling, Jianuan Zhou, Xiaofan Zhou, Lianhui Zhang
Summary: In this study, a transcriptional regulator OhrR was identified as the key regulator of virulence in D. zeae, involved in regulating zeamine production and other virulence traits such as extracellular cellulase production, biofilm formation, and swimming/swarming motility. OhrR was also found to directly regulate the transcription of key virulence genes and positively regulate the transcription of regulatory genes slyA and fis in D. zeae.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Marianne Grognot, Anisha Mittal, Mattia Mah'moud, Katja M. Taute
Summary: Cholera disease caused by Vibrio cholerae results in severe diarrhea and vomiting, with approximately 100,000 deaths per year worldwide. The bacterium's ability to swim aids in infection by potentially crossing the protective mucus barrier in the host environment. The bacterium exhibits asymmetric run-reverse-flick motility, with shorter backward runs and fluctuating swimming speeds, in all environments studied.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Junxiang Yan, Qian Liu, Xinke Xue, Jinghao Li, Yuehua Li, Yingying Su, Boyang Cao
Summary: This study reveals that the VC1795 gene plays a key role in the intestinal colonization and pathogenicity of Vibrio cholerae, and it regulates the expression of its downstream gene, VC1794, and the toxin-coregulated pilus (TCP) cluster. These findings provide insights into the regulatory mechanisms of VC1795 in bacterial pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Martina Woelflingseder, Sarah Tutz, Vera H. Fengler, Stefan Schild, Joachim Reidl
Summary: This study investigates the regulatory interaction between RpoS and RssB and their functions under bacterial starvation conditions. Phenotypic and expression analyses reveal that RssB influences bacterial motility, growth behavior, colonization fitness, and survival. Additionally, the study finds that RssB is a substrate for proteolysis, and a mutation may affect its interaction with RpoS.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Jovanka Tepavcevic, Kaiti Yarrington, Brittany Fung, Xijin Lin, Karen L. Visick
Summary: The study reveals that Hfq plays a crucial regulatory role in various phenotypes relevant to the symbiosis between V. fischeri and its squid host, including bioluminescence, motility, and biofilm formation. The deletion of Hfq not only increased luminescence production in V. fischeri but also affected motility and biofilm formation, independent of the known regulatory pathways. Hfq is thus identified as an important regulator of multiple facets of symbiosis in this system.
Article
Microbiology
Mei Zou, Kaiying Wang, Jiajun Zhao, Huifang Lu, Hui Yang, Meirong Huang, Lu Wang, Guangli Wang, Jian Huang, Xun Min
Summary: The DegS protease in bacteria activates the sigma(E) envelope stress response system, leading to the transcription of stress response genes. It is found to be involved in regulating the motility of V. cholerae. DegS regulates V. cholerae motility and chemotaxis via the cAMP-CRP-RpoS-FlhF pathway and influences the colonization of mouse intestines.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Yuehua Li, Junxiang Yan, Xueqian Guo, Xiaochen Wang, Fenxia Liu, Boyang Cao
Summary: Vibrio cholerae, a highly motile bacterium, exhibits enhanced motility through the regulation of flrA and fliK expression by ArcA, with cytR playing an intermediary role in this process.
Article
Biochemistry & Molecular Biology
Meng-Lun Hsieh, Niklas Kiel, Lisa M. Miller Jenkins, Wai-Leung Ng, Leslie Knipling, Christopher M. Waters, Deborah M. Hinton
Summary: The formation/maintenance of Vibrio cholerae biofilm is controlled by various factors, particularly the regulator VpsR and cyclic di-guanosine monophosphate (c-di-GMP). VpsR acts differently from other enhancer binding proteins (EBPs) and directly senses phosphate to determine biofilm formation/maintenance.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Evan O. Paull, Alvaro Aytes, Sunny J. Jones, Prem S. Subramaniam, Federico M. Giorgi, Eugene F. Douglass, Somnath Tagore, Brennan Chu, Alessandro Vasciaveo, Siyuan Zheng, Roel Verhaak, Cory Abate-Shen, Mariano J. Alvarez, Andrea Califano
Summary: Using a network-based approach, 407 master regulator proteins were identified to canalize individual cancer samples into 112 transcriptionally distinct tumor subtypes. These proteins were organized into 24 pan-cancer master regulator block modules regulating key cancer hallmarks and predictive of patient outcome. Genomic alterations were predicted to induce aberrant MR activity, providing insight into mechanisms linking tumor genetics and transcriptional identity.
Article
Multidisciplinary Sciences
Michaela Huber, Anne Lippegaus, Sahar Melamed, Malte Siemers, Benjamin R. Wucher, Mona Hoyos, Carey Nadell, Gisela Storz, Kai Papenfort
Summary: The study reveals the interaction between small regulatory RNAs (sRNAs) and the RNA-chaperone Hfq in Vibrio cholerae, a human pathogen. By using RIL-seq, the researchers identify multiple sRNA-mRNA interactions as well as RNA duplexes formed between two sRNA regulators. They also discover an RNA sponge called QrrX, which can bind and inactivate the Qrr1-4 sRNAs that modulate the quorum sensing pathway. The results indicate the importance of QrrX and QrrT in the rapid transition from individual to community behaviors in V. cholerae.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Manuel Maestre-Reyna, Wei-Cheng Huang, Wen-Jin Wu, Praveen K. Singh, Raimo Hartmann, Po-Hsun Wang, Cheng-Chung Lee, Takaaki Hikima, Masaki Yamamoto, Yoshitaka Bessho, Knut Drescher, Ming-Daw Tsai, Andrew H-J Wang
Summary: Vibrio cholerae switches from biofilm-bound state to planktonic state through an autocatalytic proteolysis process involving the major biofilm scaffolding protein RbmA, which is triggered by phosphate-dependent induced proximity activation mechanism. Mutants of RbmA that are defective in autoproteolysis lead to larger and mechanically stronger biofilms, indicating a new sensory role of this biofilm scaffolding element affecting microbial community homeostasis and rheological properties.
Article
Fisheries
Mery Ramirez, Cristobal Dominguez-Borbor, Lizeth Salazar, Alexis Debut, Karla Vizuete, Stanislaus Sonnenholzner, Frank Alexis, Jenny Rodriguez
Summary: This study found that using a probiotic consortium with colonization capacity can effectively control AHPND and improve the protection of shrimp larvae.
Article
Biotechnology & Applied Microbiology
Jant Cres Caigoy, Toshi Shimamoto, Asish Kumar Mukhopadhyay, Sumio Shinoda, Tadashi Shimamoto
Summary: We investigated the impact of hapR sequence mutations on biofilm formation in Vibrio cholerae. Our results demonstrate that HapR mutations strongly influence biofilm formation, with sequence polymorphisms disrupting DNA-binding sites or dimerization of HapR resulting in more robust biofilms in V. cholerae.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Ming Liu, Heng Wang, Ying Liu, Miao Tian, Zhao Wang, Run-Dong Shu, Meng-Yu Zhao, Wei-Di Chen, Hao Wang, Hui Wang, Yang Fu
Summary: Vibrio cholerae uses the Type VI secretion system (T6SS) to deliver anti-prokaryotic effectors and gain advantage in bacterial competition. In this study, a phospholipase effector called Tle1(Vc) was discovered, which is secreted by T6SS in V. cholerae. Tle1(Vc) shows antibacterial activity and induces bacterial motility by increasing the expression of flagellar-related genes independently of functional T6SS and the tit-for-tat (TFT) response.
Article
Biology
C. Neubauer, N. F. Dalleska, E. S. Cowley, N. J. Shikuma, C. -H. Wu, A. L. Sessions, D. K. Newman
Article
Multidisciplinary Sciences
Laura M. Sanchez, Andrew T. Cheng, Christopher J. A. Warner, Loni Townsley, Kelly C. Peach, Gabriel Navarro, Nicholas J. Shikuma, Walter M. Bray, Romina M. Riener, Fitnat H. Yildiz, Roger G. Linington
Article
Multidisciplinary Sciences
Nicholas J. Shikuma, Igor Antoshechkin, Joao M. Medeiros, Martin Pilhofer, Dianne K. Newman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biotechnology & Applied Microbiology
Nicholas J. Shikuma, Michael G. Hadfield
Review
Microbiology
Holger Sondermann, Nicholas J. Shikuma, Fitnat H. Yildiz
CURRENT OPINION IN MICROBIOLOGY
(2012)
Article
Microbiology
Nicholas J. Shikuma, Jiunn C. N. Fong, Lindsay S. Odell, Barrett S. Perchuk, Michael T. Laub, Fitnat H. Yildiz
JOURNAL OF BACTERIOLOGY
(2009)
Article
Microbiology
Nicholas J. Shikuma, Fitnat H. Yildiz
JOURNAL OF BACTERIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Kelly C. Peach, Walter M. Bray, Nicholas J. Shikuma, Nadine C. Gassner, R. Scott Lokey, Fitnat H. Yildiz, Roger G. Linington
MOLECULAR BIOSYSTEMS
(2011)
Article
Multidisciplinary Sciences
Petya V. Krasteva, Jiunn C. N. Fong, Nicholas J. Shikuma, Sinem Beyhan, Marcos V. A. S. Navarro, Fitnat H. Yildiz, Holger Sondermann
Article
Multidisciplinary Sciences
Nicholas J. Shikuma, Martin Pilhofer, Gregor L. Weiss, Michael G. Hadfield, Grant J. Jensen, Dianne K. Newman
Article
Microbiology
Nicholas J. Shikuma, Jiunn C. N. Fong, Fitnat H. Yildiz
Article
Cell Biology
Lara Rocchi, Charles F. Ericson, Kyle E. Malter, Sahar Zargar, Fabian Eisenstein, Martin Pilhofer, Sinem Beyhan, Nicholas J. Shikuma
Article
Biology
Charles F. Ericson, Fabian Eisenstein, Joao M. Medeiros, Kyle E. Malter, Giselle S. Cavalcanti, Robert W. Zeller, Dianne K. Newman, Martin Pilhofer, Nicholas J. Shikuma